| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 667,017 | T→G | 11.8% | E15D (GAA→GAC) | holA ← | DNA polymerase III, delta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 667,017 | 0 | T | G | 11.8% | 556.3 / 9.4 | 232 | E15D (GAA→GAC) | holA | DNA polymerase III, delta subunit |
| Reads supporting (aligned to +/- strand): ref base T (91/95); new base G (33/2); total (135/97) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 235 total aligned reads displayed.
AAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGCTTGGC > CP009273/666868‑667166 | aTCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑Ca < 1:482540/150‑1 (MQ=255) aTCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑Ca < 1:482231/150‑1 (MQ=255) cGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGT‑CAtt > 2:1994462/1‑150 (MQ=255) cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGa < 2:3850434/150‑1 (MQ=255) cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGa < 2:2080183/150‑1 (MQ=255) agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATCCGCCGCGCGCCGCCCGT‑CAATGAgcggcg > 2:447698/1‑150 (MQ=255) agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTgcg > 2:2859517/1‑150 (MQ=255) agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTgcg > 1:3852435/1‑150 (MQ=255) agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGT‑CAATGAgcggcg > 1:545386/1‑150 (MQ=255) agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGT‑AAATTAGCTgcg > 1:1801801/1‑150 (MQ=255) gtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTA‑CATTTAGCTgcgc > 1:3203224/1‑150 (MQ=255) tcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCAT‑CATTGAGCTgcgcg > 1:1882403/1‑150 (MQ=255) agtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGcgcgcg > 2:3968433/1‑150 (MQ=255) agtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCAT‑CAATGAGAGGcgcgcg > 1:4382650/1‑150 (MQ=255) gtgtTGTGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCATTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCgg < 1:1247914/150‑1 (MQ=255) tgtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGa < 2:631443/150‑1 (MQ=255) tgtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCACCCCAT‑CAATGAGCTGCGCGCGGa > 1:1501465/1‑150 (MQ=255) ttGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAgt > 2:3021664/1‑150 (MQ=255) tGGGATCAATGGACAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAgtt < 2:3234747/150‑1 (MQ=255) gggATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAgttg > 1:3810161/1‑150 (MQ=255) gATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTTAGCTGCGCGCGGAgttgtt > 1:3392892/1‑150 (MQ=255) gATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAgttgtt > 1:4713486/1‑150 (MQ=255) tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGG‑CATTGAGCGGCGCGCGGAGTTGTTACGGGt > 1:5039095/1‑150 (MQ=255) aaaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACa > 2:3683703/1‑150 (MQ=255) aaaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACa > 2:2848615/1‑150 (MQ=255) aGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCg > 2:4919430/1‑150 (MQ=255) aGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGGTACCAAGTACAAGATACGCCGCGCGCCGCCCGT‑CCTTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCg > 2:5061105/1‑150 (MQ=255) tgtgGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGaa < 1:2484318/150‑1 (MQ=255) gtggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAat < 1:1328068/150‑1 (MQ=255) tggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatc > 2:4778863/1‑150 (MQ=255) ggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatca < 2:5241837/150‑1 (MQ=255) tgtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcatc > 2:2945903/1‑150 (MQ=255) cttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGtt < 1:5090311/150‑1 (MQ=255) ttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTa < 2:645159/150‑1 (MQ=255) cGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTT‑CAATGAGCGGCGCGCGGCGTTGTTCCGGGTCCAACCGAATCATCAGTTAcc > 2:3006378/1‑150 (MQ=255) gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCAT‑CAATGAGCTGCGCGCGGAGTTGTTACGGGTACAACCGAATCATCACTTAccc > 1:2316385/1‑150 (MQ=255) aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCa > 1:3016302/1‑150 (MQ=255) aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGT‑CCTTGAGCTGCGCGCGGCGTTGTTCCGGGTACAACCGACTCATCAGTTAcccc > 2:4987805/1‑149 (MQ=255) aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTG‑CATTGAGCTGCGCGCGGAGGTGGTCCGGGTACAACCGAATCATCAGTTACCCAg > 2:3138052/1‑150 (MQ=255) tCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGc > 2:5088365/1‑150 (MQ=255) cTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgt > 2:3822939/1‑150 (MQ=255) tgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtggtg < 2:1430067/150‑1 (MQ=255) cGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGgag < 2:438029/150‑1 (MQ=255) cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAc < 2:1798735/150‑1 (MQ=255) tACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGt > 2:220387/1‑150 (MQ=255) tACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGt > 2:2979183/1‑150 (MQ=255) aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTg < 1:4377866/150‑1 (MQ=255) aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTg < 1:712938/150‑1 (MQ=255) cTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCa > 1:1272364/1‑150 (MQ=255) gACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGGTGTTCCGGGGACAACCGAATCAACAGTTACCCAGCGGGGGGGAGACGCGTGCAgg > 1:2354837/1‑150 (MQ=255) gAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGGTGCGCGCGGAGTTGTTCCGGGGACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGt > 1:766329/1‑150 (MQ=255) gAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGt < 1:1659735/150‑1 (MQ=255) cAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGc > 2:5167728/1‑150 (MQ=255) aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGcc < 1:4034605/150‑1 (MQ=255) gTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCACGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGa < 2:4295446/150‑1 (MQ=255) tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTAt < 1:1038854/150‑1 (MQ=255) ggCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATc < 1:2776226/150‑1 (MQ=255) ggCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATc < 1:4630699/150‑1 (MQ=255) cTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCg < 2:16010/150‑1 (MQ=255) ccTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCAT‑CATTGAGGGGCGCGCGGAGTTGTTCCGGGTACAACCGAAACCTCAGTTACCCAGCGTGGGGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGgtgt > 2:1751190/1‑150 (MQ=255) tGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCCGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTgg > 2:374979/1‑150 (MQ=255) cAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGGTAACCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTc > 1:3028150/1‑150 (MQ=255) tAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTT‑CATTGAGCGGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGGTGTGGTCgcc > 2:415297/1‑148 (MQ=255) cAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTc < 1:3006217/150‑1 (MQ=255) agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt < 2:4703069/150‑1 (MQ=255) agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt < 1:1334979/150‑1 (MQ=255) agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt < 2:4702899/150‑1 (MQ=255) agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt > 2:597071/1‑150 (MQ=255) agGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCATT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATACGTTGTGGTAGACGTCTGt < 1:3931192/150‑1 (MQ=255) gATCGTTACCAAGTAAAAGATACGCCGCGGGCAGCCCGT‑CATTGAGCTGCGCGCGGAGTTGTTGCGGGTACAACCGGATCATCAGTTACCCCCCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATGCGTTGTGGTCGACGTCTGttc > 2:3082242/1‑150 (MQ=255) tCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttctt < 2:2212759/150‑1 (MQ=255) cGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttcttc < 1:1953078/150‑1 (MQ=255) gTTACCAAGTAAAAGATACGCCGCGCGCCCCCCTG‑CATTGAGCGGGGCGCGGAGTTGTTACGGGTACAACCGAATCATCAGGTAACCAGCGTGGTGGAGACGCGTGCAGGCGGTGCCGGAGTATCCGTTGTGGTGGACGTCTGGTGTtcg > 2:930642/1‑150 (MQ=255) tACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtc < 1:261421/150‑1 (MQ=255) tACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtc > 2:860722/1‑150 (MQ=255) tACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtc < 1:3229119/150‑1 (MQ=255) tACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtc > 2:1547888/1‑150 (MQ=255) aCCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTTAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGGTAACCAGCCTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGGTGTGGTCGACGTCTGTTCTtcgtcg > 1:2949781/1‑150 (MQ=255) aCCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAACCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtcg > 2:1170581/1‑150 (MQ=255) aaGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGaa < 1:2904246/150‑1 (MQ=255) aaGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGaa < 1:4287455/150‑1 (MQ=255) aaGTAAAAGATACGCCGCGCGCAGCCCGT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGaa > 2:428799/1‑150 (MQ=255) aGTAAAAGATACGCCGCGCGCAGGCCTT‑AATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAAAAATAAGTTAACCAGCGTGGTGGTGTCGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGGATGTTATTAGTAGGAAc > 1:1994576/1‑150 (MQ=255) aaaGATACGCCGCGCGCCGCCCTTGCATT‑AGCTGCGCGCGGAGTTGTTGCGGGTACAACCGAATCATCCGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATACGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAat > 2:2325083/1‑150 (MQ=255) aaaGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTGACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAat < 1:447666/150‑1 (MQ=255) aaGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAata < 1:4913749/150‑1 (MQ=255) aGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAatat < 1:263042/150‑1 (MQ=255) aGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCCAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTAGTCGGAACGAatat > 2:2885146/1‑150 (MQ=255) aTACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATcc < 2:3810161/150‑1 (MQ=255) aTACGCCGCGCGCAGCCCTT‑CATTGAGCGGCGCGCGGAGTTTTTACGGGTACAACCCAAAAAAAAGTTACCCACCGTGGTGGAGACGCGTGCACGCGTTTCCGGAGTATACGGTGTGGTCGACGTCTGTTATTAGTCGGAACGAAAATcc > 2:2627035/1‑150 (MQ=255) tACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCg < 2:427460/150‑1 (MQ=255) aCGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATACGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCgc > 2:1928812/1‑150 (MQ=255) cgccgcGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCgca < 1:3163239/150‑1 (MQ=255) cgcgcgCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCgcagca > 2:4347700/1‑150 (MQ=255) gcgcgcAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCAc < 2:2727361/150‑1 (MQ=255) cgcgcAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACg < 1:2859517/150‑1 (MQ=255) gcAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGAt < 1:2704475/150‑1 (MQ=255) aGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGc > 2:2117710/1‑150 (MQ=255) aGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGc > 1:1743206/1‑150 (MQ=255) ccTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTAGTGGTCGACGTCTGATCTTCGTCGGAACGAAAATCCGCAGCACGGATGCTTg > 1:255643/1‑150 (MQ=255) | AAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTT‑CATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGCTTGGC > CP009273/666868‑667166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |