BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,282,421	A→T	12.1%	intergenic (+439/+101)	narI → / ← rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,282,421	0	A	T	12.1%	504.4 / 12.8	193	intergenic (+439/+101)	narI/rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand): ref base A (45/116); new base T (10/14); total (60/133)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGT  >  CP009273/1282273‑1282570

tcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGAc                                                                                                                                                      >  2:3652347/1‑150 (MQ=255)

    ccTTCCCCGCTTGGGCAGACTTTTTGATTTCGGAGTCGTTAGAGTATTCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTc                                                                                                                                                  <  2:275509/150‑1 (MQ=14)

    catgccccGCCGGTGCAGACTACTTCATTTCGTATTCGTTTGAGTATTCCGGGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTc                                                                                                                                                  <  2:5044905/146‑1 (MQ=14)

      ttCCGCGGTTGGGCTGAATCTTTGATTGCGGCTTCTTTTGAGATTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGa                                                                                                                                                <  1:2028608/150‑1 (MQ=14)

      gtCCCCGATTGGGCATATTTTTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGa                                                                                                                                                <  2:376325/149‑1 (MQ=14)

         cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCTGTGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                                             <  2:3229410/150‑1 (MQ=255)

         cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                                             <  1:5031419/150‑1 (MQ=255)

          ccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                                            <  1:4513900/150‑1 (MQ=255)

            tcTTGGGCAGCATTTATGTTTGCGGATTTGTTTGAGTTTTCGGTGGCTTTTGTAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                          <  2:428001/149‑1 (MQ=14)

             cTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAAACCTCTCACGCAGACGCGCCAGCCGaaa                                                                                                                                         >  1:2559439/1‑149 (MQ=14)

             cTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAc                                                                                                                                         <  1:1994231/150‑1 (MQ=255)

                  gCAGAATATTTGATTGCGGATTCGTTTGAGTATTCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAg                                                                                                                                    <  2:4891319/150‑1 (MQ=255)

                    aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                                  <  2:3063042/150‑1 (MQ=255)

                    aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGATTGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                                  <  1:2971633/150‑1 (MQ=255)

                        tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                              <  1:2764744/150‑1 (MQ=255)

                         atTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGc                                                                                                                             <  1:3652347/150‑1 (MQ=255)

                              aTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtctc                                                                                                                        <  1:2850152/150‑1 (MQ=255)

                                  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                    <  1:991340/150‑1 (MQ=255)

                                  cGGAGTAGTTTGAGATTTCGGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                    <  2:1993543/150‑1 (MQ=14)

                                   ggtttCGTTTGAGAAGTCCGGGGCTTTTGATAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCt                                                                                                                   <  1:1783160/147‑1 (MQ=255)

                                   ggATTCGTTTGAGAATTCGTTGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCt                                                                                                                   <  2:4551272/150‑1 (MQ=255)

                                       tcgactGAGATGTCCGGGTCTTATGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                               <  2:3204563/145‑1 (MQ=14)

                                        cGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                              <  2:591046/150‑1 (MQ=255)

                                        cGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                              <  1:3986438/150‑1 (MQ=255)

                                         gTTTGAGAATTCCTGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCg                                                                                                             <  1:713335/150‑1 (MQ=255)

                                            tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                          <  2:2889145/150‑1 (MQ=255)

                                                 aTTCCGTGGCTTTTGAAAGTGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                     <  1:1464318/150‑1 (MQ=255)

                                                 aTTCCGGGGCTTTTGATTGTGTTGGTGGTTGTGGAATGTTTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                     <  1:3654535/150‑1 (MQ=14)

                                                 aTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                     <  1:4222395/150‑1 (MQ=255)

                                                 aTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCCTACCCCCCAAGCGCa                                                                                                     >  2:3849928/1‑150 (MQ=14)

                                                  ttCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                                                    >  2:1213755/1‑150 (MQ=255)

                                                  ttCCGGGGCTTTAGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                                                    <  1:408528/150‑1 (MQ=255)

                                                  gtCCGGGGCTTTAGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                                                    <  1:2086660/149‑1 (MQ=255)

                                                     gtttgattttGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGTGTCGTTGCCTGCGGCAACGATCTCTCTCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                 <  1:3755032/144‑1 (MQ=11)

                                                     gggggCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                 <  1:1580062/149‑1 (MQ=255)

                                                       ggtcTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAatat                                                                                               <  1:3403388/147‑1 (MQ=255)

                                                        ggCTTTAGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGTCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAATAtt                                                                                              <  1:4181791/150‑1 (MQ=255)

                                                         gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCCACGCTCTATCGCTTTCGCTCGAGCCGCACCATATACGAAGCATAACAAACTTCCCCCCATGGGCCGAAAAttt                                                                                             >  2:4304456/1‑150 (MQ=11)

                                                          cTTTTGAAAGTGATGGTGGTGGTGGTATGATTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTg                                                                                            <  1:473184/150‑1 (MQ=11)

                                                          cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTg                                                                                            <  1:3095882/150‑1 (MQ=25)

                                                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAACTTAGTCGAAGCTTCTAATACTTCCCCGCATGGGCCGAATAATTGa                                                                                           >  2:2964407/1‑150 (MQ=11)

                                                            tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGAt                                                                                          <  2:863079/150‑1 (MQ=39)

                                                                taaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                                      <  1:3382425/149‑1 (MQ=39)

                                                                    tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAtt                                                                                  <  1:4626593/150‑1 (MQ=39)

                                                                     gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACCCGCCAGCCGAACCATTGTCGAAGCGTCTCATACATACCCCCCGGGGCCGAATAATTTATTTAGGAtta                                                                                 >  2:1480912/1‑149 (MQ=11)

                                                                     gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAAAGCTCTCTAGATGACGCTCGAGACGAACCATAGTCGAATCTTATCAACAATACCCGCATGGGCAGAAAATTTTATTTCGGAtta                                                                                 >  2:316332/1‑149 (MQ=11)

                                                                           ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCCGCTTATTTGGTTGCGGATTAGCTTga                                                                           >  2:4628430/1‑150 (MQ=14)

                                                                            gtggGGGATGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                          <  1:4279877/150‑1 (MQ=255)

                                                                              gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGagag                                                                        <  1:1107343/150‑1 (MQ=255)

                                                                               ggggAAGGATTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGt                                                                       <  1:4947394/150‑1 (MQ=255)

                                                                                 ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                                     <  1:450998/150‑1 (MQ=255)

                                                                                 ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                                     <  1:450929/150‑1 (MQ=255)

                                                                                     ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCCAAGCTTAAAATCCATACCCCCATGGGCCGAATATTTAATTGAGGATTAGGTTGGTAGTTCAggg                                                                 >  2:2059235/1‑150 (MQ=11)

                                                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                                <  1:2971049/150‑1 (MQ=255)

                                                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                                <  2:1898190/150‑1 (MQ=255)

                                                                                        ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCGGCGGCAACGCGCTCTCGCTGACGCTCGAGCCGCACCGGTGTGGAAGCGTCTAAACCGTCCCCCCCGGGGCCGAAGATTTTTTTTGGGAGTAGCTTGGGTGTTCCgggtgt                                                              >  2:4034287/1‑147 (MQ=2)

                                                                                         tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                             <  1:2352143/150‑1 (MQ=255)

                                                                                         tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGGCACGCGCTCTCGCTGGAGCGCGAGCCGAACCATAGTCCAAGCGTCTCATACATACCCCCCGGGGGCGAAAATTTGATTGCGGGTTAGCGTGGGAGTTCAggggcgt                                                             >  2:3626493/1‑146 (MQ=9)

                                                                                         tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACGCACTCTCGCTGTCGCGCGCGCCGTACCATAGGAGTAGCTTCTCAACCATCCCCCCCAGGGCCGCATATTTTATTTCGGATTCGGTGGTGAGTTCAGGGCCta                                                             >  2:2295530/1‑149 (MQ=2)

                                                                                          aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                            <  1:3029100/150‑1 (MQ=255)

                                                                                            tcttcgcTGCGCGATTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                                          <  1:4294353/146‑1 (MQ=255)

                                                                                            tccgcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                                          <  1:1660086/147‑1 (MQ=255)

                                                                                            tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCGGACGCTCGAGCCGAACCATAGTCGAAGCATCACATCCATCCCCGCAAGGGCAGCATATTTAATTGCGGCTTCGGTGGGGAGGTAAGGGGCgtttt                                                          >  2:2505447/1‑149 (MQ=11)

                                                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGa                                                         <  2:1277685/150‑1 (MQ=255)

                                                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGa                                                         <  2:816303/150‑1 (MQ=255)

                                                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGa                                                         <  2:4402878/150‑1 (MQ=255)

                                                                                                    gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAt                                                  <  1:47897/150‑1 (MQ=255)

                                                                                                    gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAt                                                  <  2:3345669/150‑1 (MQ=255)

                                                                                                       cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggt                                               <  1:2944223/150‑1 (MQ=255)

                                                                                                         cTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCCCGCTCCCTGCCGCCCGAGCCGCACCCTAGTCGGCTCTTATCATACCTCCCCCCCGGGGCCCCAGATTTGTGTGCGGGTTCGCGTGAGAGGTGAGGGGCTGTTGGTAGTGAgggggg                                             >  2:2661266/1‑150 (MQ=255)

                                                                                                          ttCGCCCTTCGTGTCGTTGCGTGGGGCATCGATCTCTCGCTGTCGCTCGAGCGTTACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                                            <  1:8328/150‑1 (MQ=14)

                                                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                                            <  1:3803234/150‑1 (MQ=255)

                                                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                                            <  1:10085/150‑1 (MQ=255)

                                                                                                           tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                           <  1:911954/150‑1 (MQ=255)

                                                                                                           tCGCCCTTCGGGTCGTTGCCAGCGGCAACGCGCACTCGCTGACGCGCGCGCCGAACCTTAGTCGAAGAATATCATCCATCCCCCCATGGGCAGAATATTTTATTTGGGATTAGGGGTGGAGTTGAGGGGGATTTTTAAgtggtggtggtg                                           >  2:1727036/1‑150 (MQ=2)

                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCCAACCGTAGTAGAAGCTTATCAACCATACCCCCAAGGGGAGAATAATTTATTTCGGAATAGCTGGGTAGTTCAAGGACTTTTGAAAGTGATGGTGGTggg                                         >  2:2569534/1‑150 (MQ=2)

                                                                                                                 ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                     <  1:1307786/150‑1 (MQ=11)

                                                                                                                   cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                   <  1:3465173/150‑1 (MQ=255)

                                                                                                                      gTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                <  1:3588568/150‑1 (MQ=255)

                                                                                                                      gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                <  1:2843607/150‑1 (MQ=255)

                                                                                                                        cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTCATTGCGGCTTCGTTGGGGAGGTCAGGGACTTTTGCAAGTGCTGGTGGTGGGGGAAGGAttcg                              >  2:4042954/1‑148 (MQ=11)

                                                                                                                        cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGGAAGTGATGGTGGTGGGGGAAGGATTAc                              <  1:3186744/150‑1 (MQ=255)

                                                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACTCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                             <  2:3634354/150‑1 (MQ=255)

                                                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                             <  1:3668956/150‑1 (MQ=255)

                                                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                           <  2:4692725/150‑1 (MQ=255)

                                                                                                                            gCCTGCGGCAACGCGCTCTCGCTCACGCTCGCGCAGAACCTTAGTAGAAGCCTCTCCTCCTTCCCCGCATGGGCAGATGAAATTATTGCGGAGTCGCTTGAGTGTTCCGGGACTTATGAAAGTGATGGTGGTGGGGGAAGGATTACTCAg                          <  2:2661063/150‑1 (MQ=14)

                                                                                                                                     aaCTCTCTCTCGCTGACGCTCCAGACTTAACTTATTAGAAGCTTCTCATCATTCCCCGCATGGGAAGAATATTTGATTGCGTATTAGATTGAGTTTTCAGTGAATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                 <  2:3410573/150‑1 (MQ=14)

                                                                                                                                     aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                 >  1:1516958/1‑150 (MQ=255)

                                                                                                                                     aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                 >  1:1517362/1‑150 (MQ=255)

                                                                                                                                      aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGCTGGAGAGGTCAGGGACTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCACCGCGacgcgc                >  2:2713917/1‑150 (MQ=14)

                                                                                                                                       cGCACTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt               <  2:624232/150‑1 (MQ=255)

                                                                                                                                        gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt              <  1:1199901/150‑1 (MQ=255)

                                                                                                                                        gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt              >  2:5237605/1‑150 (MQ=255)

                                                                                                                                          tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg            >  1:262174/1‑150 (MQ=255)

                                                                                                                                           ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAAGGGCAGACTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGCCTTTTGAAAGTGATGGGGGTGGGGGGAGGATTACTCAGCGCTGCGCGCTTCGc           >  1:4393523/1‑150 (MQ=17)

                                                                                                                                             ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGccc         >  1:4872942/1‑150 (MQ=255)

                                                                                                                                               cGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGAGAGCTTCGCCCta       >  2:2847522/1‑149 (MQ=255)

                                                                                                                                                gCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc      >  1:1017407/1‑150 (MQ=255)

                                                                                                                                                  tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg    >  1:2235423/1‑150 (MQ=255)

                                                                                                                                                  tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg    >  2:3508555/1‑150 (MQ=255)

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGT  >  CP009273/1282273‑1282570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`