Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,701,282 A→C 7.7% Q36P (CAG→CCG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,701,2820AC 7.7% 649.8 / 6.2 282Q36P (CAG→CCG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (149/98);  new base C (28/3);  total (177/105)
Fisher's exact test for biased strand distribution p-value = 6.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

GATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCT  >  CP009273/1701133‑1701431
                                                                                                                                                     |                                                                                                                                                     
gATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCa                                                                                                                                                       <  1:393346/150‑1 (MQ=255)
     gAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAc                                                                                                                                                  <  2:3666524/150‑1 (MQ=255)
      aaCACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcc                                                                                                                                                 <  1:4632336/150‑1 (MQ=255)
          ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTg                                                                                                                                             >  1:2578449/1‑150 (MQ=255)
          ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTg                                                                                                                                             <  2:3101051/150‑1 (MQ=255)
          ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTg                                                                                                                                             <  1:3821264/150‑1 (MQ=255)
             ttCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCTCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgcg                                                                                                                                          >  2:3007444/1‑150 (MQ=255)
             ttCCCGTGCGTATCAGTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgcg                                                                                                                                          <  1:4061398/150‑1 (MQ=255)
               cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCa                                                                                                                                        <  1:4966162/150‑1 (MQ=255)
                    gCGTATCATTCCCGTGGAAAACCATGCTTATTTTATTCTCTGAATTAAGAAAAAATAAAATCTGGTATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtt                                                                                                                                   <  1:597879/150‑1 (MQ=255)
                    gCGTATCATGCCCGTGGAACACCATGCTTATTTTATTCTCTGCATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtt                                                                                                                                   <  1:3221011/150‑1 (MQ=255)
                      gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCgggtt                                                                                                                                 >  1:421353/1‑146 (MQ=255)
                      gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtttt                                                                                                                                 <  2:222073/150‑1 (MQ=255)
                       tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                >  1:2100063/1‑150 (MQ=255)
                       tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                >  1:660173/1‑150 (MQ=255)
                       tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTAAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGCTACCCCAGGCGCAGcctgttt                                                                                                                                >  1:4729527/1‑144 (MQ=255)
                         tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGtt                                                                                                                              >  1:1952649/1‑150 (MQ=255)
                           aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTAt                                                                                                                            >  2:74005/1‑150 (MQ=255)
                               ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGGTTTTTTATTCCa                                                                                                                        >  1:4644155/1‑150 (MQ=255)
                               ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGGTTTTTTTTTACa                                                                                                                        >  1:4644787/1‑150 (MQ=255)
                               ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGAACACCCCTGCGCCACGGACCCCAGGAGCCACGATTTTTTATTACa                                                                                                                        >  1:1579490/1‑150 (MQ=255)
                                  tGGAACACCATGCTTAAGTTTTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                                                                     <  1:1732280/150‑1 (MQ=255)
                                   ggAACACCTTGCTTACTTTATTCCCTCCATTCACAAAAAATAAACGGGGGTCTTACAACGGCGGCATCCATCCACCGGAGATTAAAACCCAGTCCAACGGTACACCCCGGCGCCTGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGa                                                                                                                    <  1:4900700/150‑1 (MQ=255)
                                    gAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCCGCGTTGTGTTATTCCACAGaa                                                                                                                   >  2:2691569/1‑150 (MQ=255)
                                       cacCTTGCTTATTTTATCCTCTGCATTAAGAAAAAATAAAAACTGGTATTTCAACGGCGGCATCCACCCACCGGAGATTAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                                                                                <  1:558160/150‑1 (MQ=255)
                                        acCATGCTTACTTTATTCTCTCAATACACAAAAAATAAAATCTGGGATTTCCACGGCGGCATCCATCCACCGTAGATGAAAACCCAGTCCACCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                               <  1:101307/150‑1 (MQ=255)
                                        acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTAGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTGTGTTATTCCACTGAAACAg                                                                                                               >  2:1618072/1‑150 (MQ=255)
                                           aTGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGCATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCat                                                                                                            <  2:571155/150‑1 (MQ=255)
                                               ttattttATACTCTTCATTAATAAAAAAAAAAATCGGGGATTTCCACGGCGGCATCCATCCACCGTATATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                        <  1:983973/145‑1 (MQ=255)
                                               ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                        >  2:2665184/1‑150 (MQ=255)
                                                tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                       >  2:315289/1‑150 (MQ=255)
                                                tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGACCCCAGGCGCCGCGTTTTGTTATTACACAGAAACCGCATATTgg                                                                                                       >  1:263093/1‑150 (MQ=255)
                                                 aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgc                                                                                                      >  2:4293073/1‑150 (MQ=255)
                                                  aGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACAGAAACAGCAGATTGgcg                                                                                                     >  2:90914/1‑150 (MQ=255)
                                                   gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                    >  2:283279/1‑150 (MQ=255)
                                                   gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                    >  2:2138913/1‑150 (MQ=255)
                                                    ttATTCTCTGCATTCAGAAAAAATAAAATCGGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCTGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCt                                                                                                   <  2:3511348/150‑1 (MQ=255)
                                                    ttATTCTCTGCAGTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCt                                                                                                   <  2:4579676/150‑1 (MQ=255)
                                                     tATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTg                                                                                                  >  1:1233648/1‑150 (MQ=255)
                                                      aTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCAGCCATCCACCGGAGATGAAAACCCAGTCCACCGGGACCCCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGa                                                                                                 <  2:2267954/150‑1 (MQ=255)
                                                          tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                                                                             <  2:3755922/150‑1 (MQ=255)
                                                          tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                                                                             >  2:2711948/1‑150 (MQ=255)
                                                          tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTACCCCAGGCGCAGCGGTTTGTTATTACAAGGAAAAAGCATATTGGCGCTGAAGGt                                                                                             >  1:2390173/1‑150 (MQ=255)
                                                           ctGAATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGATATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                            <  1:238358/150‑1 (MQ=255)
                                                            ttcATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                                                                           <  1:1918044/148‑1 (MQ=255)
                                                            tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGa                                                                                           >  2:1193622/1‑150 (MQ=255)
                                                               aTTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt                                                                                        >  2:345833/1‑150 (MQ=255)
                                                                 tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgt                                                                                      >  2:2610827/1‑150 (MQ=255)
                                                                   aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                    >  2:4963086/1‑150 (MQ=255)
                                                                   aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                    >  2:4963048/1‑150 (MQ=255)
                                                                   aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                    >  2:1833282/1‑150 (MQ=255)
                                                                      aaaaaTAAAATCTGGCATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCTGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGtc                                                                                 <  1:4209709/150‑2 (MQ=255)
                                                                       aaaataaaatCGGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTa                                                                                <  1:3924184/150‑1 (MQ=255)
                                                                         aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGc                                                                              >  2:1451579/1‑150 (MQ=255)
                                                                         aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAACCCAGGCGCAGCGTTTTGTTATTCCACAGTAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGc                                                                              >  1:1290593/1‑150 (MQ=255)
                                                                          ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                                                                             <  1:3339322/150‑1 (MQ=255)
                                                                          ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACGGAAACAGCAGATTGGCGCGGAAGGTGAGTTGTGCGTTAGCg                                                                             >  2:1340544/1‑150 (MQ=255)
                                                                             aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGCGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGGTAGCGTCg                                                                          >  2:2523766/1‑150 (MQ=255)
                                                                              aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCCTCTTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCgg                                                                         >  2:4120670/1‑150 (MQ=255)
                                                                              aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTGGCCTGAAACAGCATGTTGGCGCTGTAGGGGAGTTGTGCGTTTGcggcgg                                                                         >  1:141594/1‑150 (MQ=255)
                                                                                tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCg                                                                       <  1:283279/150‑1 (MQ=255)
                                                                                tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCCGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGTTGTGGGTTAGCGTCGGCg                                                                       >  1:4114333/1‑150 (MQ=255)
                                                                                    ggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCCGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATaa                                                                   >  1:5129194/1‑150 (MQ=255)
                                                                                      aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGTTTTGTTATTGCCCTGAAACAGCATATTGGCGCGGAAGGTGAGTTTTGCGTTAGCGTCGGCGATAAAg                                                                 >  1:296491/1‑150 (MQ=255)
                                                                                      aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                                                 >  2:4291033/1‑150 (MQ=255)
                                                                                      aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                                                 >  2:4693401/1‑150 (MQ=255)
                                                                                      aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAAATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGCTAAAg                                                                 >  2:2325850/1‑150 (MQ=255)
                                                                                           aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGGGCCCCGTACCCCGGGCGCCGCGGGGTGTTTTTCCACTGTAACAGCCGAGTGGCGCGGCCGGGGGGGGGTGCGTTTGCGGCGGGGCTGAAGGATTg                                                            >  1:112039/1‑150 (MQ=255)
                                                                                           aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                            <  1:3693991/150‑1 (MQ=255)
                                                                                           aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAAACCAGGCCCAGCGTTTTTTTATTACACAGAAAAAGCATATTGGCGCTTAAGGTTTGTTGTGAGTTAGCGTCGGCGATAAAGTAttt                                                            >  1:3057072/1‑149 (MQ=255)
                                                                                            aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                                           <  2:5103529/150‑1 (MQ=255)
                                                                                                cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCgg                                                       >  2:974703/1‑150 (MQ=255)
                                                                                                   catccatcCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTTTTATTACACTGAAACAGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAATTATTGCGCGGCCa                                                    >  1:1663224/1‑150 (MQ=255)
                                                                                                        atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACGGACACGGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATCAAGTCTTGCGCGGCCAGCCGc                                               >  2:1980676/1‑150 (MQ=255)
                                                                                                          ccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCtt                                             <  1:1649971/150‑1 (MQ=255)
                                                                                                               ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGTTTTGTTTTTGCACTGAAACAGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGCTAAAGTATTTCGCGGCCAGCCGCGTAcccc                                        >  2:3076967/1‑149 (MQ=255)
                                                                                                               ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGGCGCTGTAGGTGAGTTGTTCGTTAGCGTCGGCGGTAAAGTATTTCGCGGCCAGCCGCGTAcccc                                        >  2:4827658/1‑149 (MQ=255)
                                                                                                                 agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCCCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTg                                      <  2:4209691/150‑1 (MQ=255)
                                                                                                                 agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGGTTTGTTATTCCACTGAAACAGCAAATTGGCGCTGAAGGTGTGTTGTGGGTTAGGGTCGGGGATAAAGTAATTGGCGGCCAGCCGCGTACCCGTg                                      >  1:2428483/1‑150 (MQ=255)
                                                                                                                    tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACCGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTc                                   >  2:1030041/1‑150 (MQ=255)
                                                                                                                    tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTTTTATTACACAGAAAAACCATATTTGCGCTGAAGGTGAGTTGTGCGTTATCGTCGGCGATAAAGTATTGCGCGGCCCGCCGCGTACACCTGGTc                                   >  1:3739856/1‑150 (MQ=255)
                                                                                                                     gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcg                                  >  2:3813530/1‑150 (MQ=255)
                                                                                                                       aaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcgcg                                >  2:4918034/1‑150 (MQ=255)
                                                                                                                       aaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcgcg                                <  2:4934541/150‑1 (MQ=255)
                                                                                                                       aaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCATATTGGCGCAGAAGGGGAGTTGTGCGTTCGCGTCGGCGATAAAGTCTTGCGCGGCCAGCCGCTTACCCGGGGGcgcg                                >  2:1276468/1‑150 (MQ=255)
                                                                                                                           ccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAGTGGCGCTGAAGGTGAGTTGTGCGGTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCaa                            >  2:2843396/1‑150 (MQ=255)
                                                                                                                              gTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAt                         <  1:2711948/150‑1 (MQ=255)
                                                                                                                                    cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                   <  2:2908977/150‑1 (MQ=255)
                                                                                                                                     ggTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCt                  <  2:1503483/150‑1 (MQ=255)
                                                                                                                                      gTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTg                 <  1:4875829/150‑1 (MQ=255)
                                                                                                                                          acCCCTGCGCCAGGTACCCCTGGCTCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTGAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCa             <  2:1646402/150‑1 (MQ=255)
                                                                                                                                          acCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAGTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCa             <  2:4281513/150‑1 (MQ=255)
                                                                                                                                              cTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgcg         <  2:3623687/150‑1 (MQ=255)
                                                                                                                                                 cgcCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGccc      <  2:4071789/150‑1 (MQ=255)
                                                                                                                                                  gcCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCa     >  2:3412554/1‑150 (MQ=255)
                                                                                                                                                   ccAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAc    >  1:3553476/1‑150 (MQ=255)
                                                                                                                                                   ccAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAc    >  2:5063004/1‑150 (MQ=255)
                                                                                                                                                   ccAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAc    >  2:3254337/1‑150 (MQ=255)
                                                                                                                                                     aGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCt  <  1:477231/150‑1 (MQ=255)
                                                                                                                                                     aGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCt  <  1:2241215/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCT  >  CP009273/1701133‑1701431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑