Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,469 A→C 8.5% D421E (GAT→GAG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4690AC 8.5% 997.5 / 8.2 376D421E (GAT→GAG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base A (117/209);  new base C (43/2);  total (165/211)
Fisher's exact test for biased strand distribution p-value = 2.06e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 384 total aligned reads displayed.

TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG  >  CP009273/3721321‑3721617
                                                                                                                                                    |                                                                                                                                                     
tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCcacag                                                                                                                                                      >  2:2959774/1‑149 (MQ=255)
 ttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c                                                                                                                                                    <  2:344010/150‑1 (MQ=255)
   ctcGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑ccc                                                                                                                                                  >  2:1834360/1‑150 (MQ=255)
   ctcGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccc                                                                                                                                                  <  2:1727116/150‑1 (MQ=255)
    tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑cccc                                                                                                                                                 >  2:3231437/1‑150 (MQ=255)
         gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCg                                                                                                                                            >  1:950093/1‑150 (MQ=255)
          tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑cccccccccg                                                                                                                                           <  2:3056144/150‑3 (MQ=255)
          tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGt                                                                                                                                           <  2:5007333/150‑1 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGt                                                                                                                                      <  1:2128711/150‑1 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGt                                                                                                                                      <  1:3042301/150‑1 (MQ=255)
                 tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTaa                                                                                                                                    <  1:3808172/150‑1 (MQ=255)
                   aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACgggggtg                                                                                                                                  >  1:3393433/1‑145 (MQ=255)
                     cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGa                                                                                                                                <  2:2133246/150‑1 (MQ=255)
                       gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgc                                                                                                                              <  2:2237353/150‑1 (MQ=255)
                        cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgct                                                                                                                             <  2:2523710/150‑1 (MQ=255)
                          tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTGAGCGAgcggc                                                                                                                           >  1:4322126/1‑150 (MQ=255)
                            aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGctgctg                                                                                                                         <  2:4915062/150‑1 (MQ=255)
                             cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                        <  1:4878182/150‑1 (MQ=255)
                             cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                        <  1:2715284/150‑1 (MQ=255)
                                        tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCCCCGGCCGGTACTCGCGCTGCGGACCGcggctctc                                                                                                             >  1:1472746/1‑143 (MQ=255)
                                         ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CGCCCCCCGGAGGGGGAGCGTGCTGCTGGGCGCTGTGATcc                                                                                                            >  2:4103400/1‑150 (MQ=255)
                                             gATGCGACTGTTCTAACGGTAGTTGTGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                        <  1:45049/150‑1 (MQ=255)
                                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                       <  1:3502066/150‑1 (MQ=255)
                                                    cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATcgg                                                                                                 >  1:3369018/1‑148 (MQ=255)
                                                     tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGTAATCGGGCTGCTGACCGCGGGTGTCCGCCAGCAGCTGa                                                                                                >  1:1318558/1‑150 (MQ=255)
                                                      gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAGCGAGCGGCTGCCGGCTGGTATCCGCCCGCATCGGAc                                                                                               >  2:565648/1‑150 (MQ=255)
                                                      gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGTCGGTAATCGTGCTGCTGACCGCTGATAGCCGCCCGCATCTGAc                                                                                               >  2:3876801/1‑150 (MQ=255)
                                                         cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGGGCTGCTGTCCGCTGATGTGCGCCAGCCTCTGACGcc                                                                                            >  1:3110998/1‑150 (MQ=255)
                                                         cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGCCTCGAGCTGCGGGCCGGTGAGATCCGCCAGCATCGGGCGcc                                                                                            >  1:1106783/1‑150 (MQ=255)
                                                          tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCGCCGGACGGGAATCGGGCTGCTGGCCGCGGGGCTCCGCCGGCCGCGGACGccc                                                                                           >  2:3678329/1‑149 (MQ=255)
                                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                      <  2:1094273/150‑1 (MQ=255)
                                                                   ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                  <  1:4441083/150‑1 (MQ=255)
                                                                    ggCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                 <  2:3247062/149‑1 (MQ=255)
                                                                     gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                <  1:5096660/150‑1 (MQ=255)
                                                                      cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTTCGGTTGTCGGGCTGCTGACCGCGGATCTCCGCCAGCGTCTGACGCCAGTACTCACTAcg                                                                               >  1:4198436/1‑150 (MQ=255)
                                                                      cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                                                                               <  1:916849/150‑1 (MQ=255)
                                                                         caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CGCCCCCCGTGCGGTGATCGGGCTGCTGGCCGCGGTTCTGCGCGCGCCGCTGGCGCCAGTACTCCCTACgcgc                                                                            >  1:2968886/1‑150 (MQ=255)
                                                                          aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                           <  2:355908/150‑1 (MQ=255)
                                                                          aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAGCGAGCGGCTGAGCGCTGGTATCCGCCAGCAGCTGACGCCAGTTCTCAGTCCGcgcc                                                                           >  1:2557134/1‑150 (MQ=255)
                                                                            caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                         <  2:280721/150‑1 (MQ=255)
                                                                            caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                         <  2:4563241/150‑1 (MQ=255)
                                                                             aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                                        <  1:3656634/150‑1 (MQ=255)
                                                                             aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGGATCGAGCTGGGGCCCGCTGGTGGCCGCCAGCCTCGGACGCCAGGGCTCTCGGCGcgccccg                                                                        >  1:759407/1‑150 (MQ=255)
                                                                             aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                                        <  1:4880292/150‑1 (MQ=255)
                                                                                   aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGAGTCGAGGTGGGGACCGCGGATATCCGCCAGCCTCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCa                                                                  >  1:3869797/1‑150 (MQ=255)
                                                                                    tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                 >  1:1689959/1‑150 (MQ=255)
                                                                                    tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTACGGGAATCGAGCTGCTGGCCGCGGGTATCCGCCCGCCTCTGACGCCCGTACGCACTGCGCGCCCCGcccccca                                                                 >  2:5197473/1‑148 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                <  1:4720566/150‑1 (MQ=255)
                                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGAGCGCTGTTCTCCGCCAGCTTCTGACGCCAGTACTCACTCCGCGCCCCGccccccgtc                                                               >  2:4168058/1‑146 (MQ=255)
                                                                                       gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                              <  1:1646583/150‑1 (MQ=255)
                                                                                       gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                              <  1:2056540/150‑1 (MQ=255)
                                                                                        cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGGCGGTACTCGCGCGGCTGACCGCGGGTATCCGCGCGCAGCTGACGCCCGTCCGCCCTGCGCGCCCCGccccccctcga                                                             >  2:4609863/1‑144 (MQ=255)
                                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTCCGGTACTCGGGGGGCTGTCCGGGGCTATCCGCCAGCCTCTGACGCCAGTACTGACTACGCGCCCCGcccccccgggac                                                            >  1:679412/1‑143 (MQ=255)
                                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                            <  1:4742558/150‑1 (MQ=255)
                                                                                         gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                            <  1:575079/150‑1 (MQ=255)
                                                                                          aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                                           <  1:2355011/150‑1 (MQ=255)
                                                                                           tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                          <  1:4981761/150‑1 (MQ=255)
                                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAAGCGAGCTGCTGACCGCTGATATCCGCCAGCGGCTGACGCCAGTACTCACTCCGCGCCCCGCCCCCCCTCAACGtc                                                         >  2:4360218/1‑149 (MQ=255)
                                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                    <  1:401348/150‑1 (MQ=255)
                                                                                                  ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                   <  1:2438600/150‑1 (MQ=255)
                                                                                                  ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                   <  2:1551119/150‑1 (MQ=255)
                                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                  <  2:5112472/150‑1 (MQ=255)
                                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                  <  1:4401863/150‑1 (MQ=255)
                                                                                                     ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                <  1:100515/150‑1 (MQ=255)
                                                                                                      tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                               <  1:4635084/150‑1 (MQ=255)
                                                                                                       cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGGCGTCGTGCTGCTGTCCGCTGAGTTCCGCCCGCATCTGACGCCGGTCCTCACTCCGCGCCCCGCCCCCCATCCACGGCACCCTGTGCgg                                              >  2:3381675/1‑150 (MQ=255)
                                                                                                         ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCCGCATCTGACGCCCGTACTCGCTCCGCGCCCCGCCCCCAGTCAACGTCAGACGTTGCGGtt                                            >  2:1329248/1‑150 (MQ=255)
                                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                           <  1:2520942/150‑1 (MQ=255)
                                                                                                            cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                         <  1:2874739/150‑1 (MQ=255)
                                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGTGCGCGGGCTGCTGGCCGCTGTTCTCCGCCAGCATCTGGCGCCGGGTCTCGCTTCGCGCCCCGCCCCCCAGCGACGGAACGCGGTGCGGGTTAAt                                        >  2:864736/1‑150 (MQ=255)
                                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGGCGGTCCTCGCGCTGCTGTCCGCGGATATCCGCCCGCAGCTGCCGCCAGTCCTCCCTCCGCGCCCCGCCCCCACGCAACGTCACACGTTGCGGTGTAAt                                        >  2:4861966/1‑150 (MQ=255)
                                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGGAATCGTGCTGCTGTCCGCGGATCTCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGACCCGTTGCGGTTTAATACCg                                    >  1:545620/1‑150 (MQ=255)
                                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                    <  1:2838920/150‑1 (MQ=255)
                                                                                                                    gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTCCGGGCATCGCGCTGCGGGCCGCGGTTATCCGCCAGCGTCTGCCGCCAGTGCTCACTGCGCGCCCCGCCCCCCAGCAACGGAACACGTTGCGGTGGAATACCGCAg                                 >  2:1967268/1‑150 (MQ=255)
                                                                                                                    gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTCACACGTTGCGGTTTAATACCGCAg                                 >  1:3177598/1‑150 (MQ=255)
                                                                                                                    gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                 <  1:870033/150‑1 (MQ=255)
                                                                                                                    gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                 <  2:461700/150‑1 (MQ=255)
                                                                                                                     ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                <  1:4171228/150‑1 (MQ=255)
                                                                                                                       aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGCCCGGGGATATCCGCCAGCATCTGACGCCCGGACTCACGACGCGCCCCGCCCCCCATCAACGTGACACTGTGCGGGTTAGTACCGCAGGcc                              >  2:493460/1‑149 (MQ=255)
                                                                                                                       aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTGCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTGATACCGCAGGCa                              >  1:1891063/1‑150 (MQ=255)
                                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                             <  1:1201260/150‑1 (MQ=255)
                                                                                                                          cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTAGGGTAATGGTGCTGGTGACCGCTGTTATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAGCACTGTGCGGTTTTTTACCGGGGGCGTGc                           >  2:1236755/1‑150 (MQ=255)
                                                                                                                          cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGc                           >  1:3536018/1‑150 (MQ=255)
                                                                                                                             gctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATTTCCGCCAGGATCTGACGGCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCCCg                        >  2:2481675/1‑150 (MQ=255)
                                                                                                                             gctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGGATCGAGCTGCTGTCCGCTGAGAGCCGCCCGCCTCGGACGCCCGTACGCACTACGCGCCCCGCCCCCACTCAACGGAACCCGGTGCGGTTGAATACCGCCGGCAGGCCCg                        >  2:4713568/1‑150 (MQ=255)
                                                                                                                                gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                     <  2:4416098/150‑1 (MQ=255)
                                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATTCACGACAt                    <  1:3341861/150‑1 (MQ=255)
                                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCAGCAACGTAACACTGTGCGGTTTAATACCGCAGGCATGCACGACAt                    >  2:1362952/1‑150 (MQ=255)
                                                                                                                                 cgcCCAGGGCTGGCCCCACATCCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                     <  2:655452/150‑1 (MQ=255)
                                                                                                                                  gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                   <  1:1028723/150‑1 (MQ=255)
                                                                                                                                     cAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                <  2:2765794/150‑1 (MQ=255)
                                                                                                                                     cAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTCACACGTTGCGGTGTAATACCGCAGGCATGCACGACATCCAt                >  2:1490991/1‑150 (MQ=255)
                                                                                                                                      aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg               >  2:1769796/1‑150 (MQ=255)
                                                                                                                                      aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAGGCACGACATCCATg               >  2:1000760/1‑150 (MQ=255)
                                                                                                                                          cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTGTAATACCGCAGGCCTGCACGACATCCATGccct           >  2:1684300/1‑148 (MQ=255)
                                                                                                                                          cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt           >  2:1433693/1‑150 (MQ=255)
                                                                                                                                          cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt           <  1:1362237/150‑1 (MQ=255)
                                                                                                                                                ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa     >  1:4741163/1‑150 (MQ=255)
                                                                                                                                                 cacaT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg    >  2:3825616/1‑150 (MQ=255)
                                                                                                                                                 cacaT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg    >  1:2170175/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG  >  CP009273/3721321‑3721617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑