Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,480 T→G 8.1% T418P (ACG→CCG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4800TG 8.1% 1128.3 / 24.0 380T418P (ACG→CCG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (131/208);  new base G (37/3);  total (169/211)
Fisher's exact test for biased strand distribution p-value = 2.82e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  CP009273/3721331‑3721629
                                                                                                                                                      |                                                                                                                                                     
     gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGt                                                                                                                                                  >  1:4389548/1‑150 (MQ=255)
     gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTAGGGt                                                                                                                                                  >  2:1562948/1‑150 (MQ=255)
      aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTa                                                                                                                                                 >  1:957632/1‑150 (MQ=255)
       tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑CCCCCCCCGTGCgggag                                                                                                                                                >  1:1739055/1‑147 (MQ=255)
        aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAt                                                                                                                                               <  1:1574713/150‑1 (MQ=255)
         aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATc                                                                                                                                              <  1:3794530/150‑1 (MQ=255)
         aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATc                                                                                                                                              <  2:3953308/150‑1 (MQ=255)
         aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTACGGTAGTc                                                                                                                                              >  1:3193531/1‑150 (MQ=255)
           cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGa                                                                                                                                            <  2:2133246/150‑1 (MQ=255)
             gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgc                                                                                                                                          <  2:2237353/150‑1 (MQ=255)
              cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgct                                                                                                                                         <  2:2523710/150‑1 (MQ=255)
               aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑GCCCCCCGGTCCGGGACTCgcgcgg                                                                                                                                        >  1:3343817/1‑148 (MQ=255)
                tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCGCCCCCGTACGGTCAGCGGgctgc                                                                                                                                       >  1:4659405/1‑150 (MQ=255)
                 aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGCCGGTAGTCGGgctggt                                                                                                                                      >  1:5151855/1‑148 (MQ=255)
                  aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGctgctg                                                                                                                                     <  2:4915062/150‑1 (MQ=255)
                   cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                                    <  1:4878206/150‑1 (MQ=255)
                     cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑GCCCCCCCGTACGGGACTGGGGCGGCTGagg                                                                                                                                  >  1:273917/1‑148 (MQ=255)
                        cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCTGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                               <  1:3386317/150‑1 (MQ=255)
                        cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                               <  1:4913156/150‑1 (MQ=255)
                                gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCCCCCCGTCGGGGAGTCGTGCTGGTGGGCGCTGctaggcg                                                                                                                       >  2:13969/1‑146 (MQ=255)
                                 tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGGATCGCGCTGCTGGCCGGTGaggtgcgc                                                                                                                      >  1:3552542/1‑143 (MQ=255)
                                    aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                   <  1:3502232/150‑1 (MQ=255)
                                    aTGCGACTGTTCTAACGGTAGTTGCGGCAACAAGTCCATGAGCGAATTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCGGACCGCTGATATCCGCCAg                                                                                                                   <  2:4237812/150‑1 (MQ=255)
                                      gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCa                                                                                                                 >  2:3398716/1‑150 (MQ=255)
                                        gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCATCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTGCGGGCATCGGGCGGCTGGCCGCGGAGATCCGCCAGCagc                                                                                                               >  2:4115235/1‑148 (MQ=255)
                                            gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGAc                                                                                                           <  2:1861211/150‑1 (MQ=255)
                                            gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAGCGAGCGGCTGCCGGCTGGTATCCGCCCGCATCGGAc                                                                                                           >  2:565648/1‑150 (MQ=255)
                                               cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGCCTCGAGCTGCGGGCCGGTGAGATCCGCCAGCATCGGGCGcc                                                                                                        >  1:1106783/1‑150 (MQ=255)
                                                tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCGCCGGACGGGAATCGGGCTGCTGGCCGCGGGGCTCCGCCGGCCGCGGACGccc                                                                                                       >  2:3678329/1‑149 (MQ=255)
                                                tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                       <  1:4806626/150‑1 (MQ=255)
                                                  aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                                                     <  1:2385909/150‑1 (MQ=255)
                                                    ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                                                   <  1:555454/150‑1 (MQ=255)
                                                         ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                              <  1:4441083/150‑1 (MQ=255)
                                                          tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                             <  2:3245065/150‑1 (MQ=255)
                                                            cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTT‑CCCCCCCCGTACGGTCATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGGCGCCCGTTCTCACTAcg                                                                                           >  2:1046321/1‑150 (MQ=255)
                                                            cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                                                                                           <  1:917142/150‑1 (MQ=255)
                                                                aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAGCGAGCGGCTGAGCGCTGGTATCCGCCAGCAGCTGACGCCAGTTCTCAGTCCGcgcc                                                                                       >  1:2557134/1‑150 (MQ=255)
                                                                  caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                     <  1:4202701/150‑1 (MQ=255)
                                                                  caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                     <  2:4562860/150‑1 (MQ=255)
                                                                   aaTTCAATGAGCGATTTCTCTGGATTCGCCGCTATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                                                    <  1:219744/150‑1 (MQ=255)
                                                                     ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                  <  1:779131/150‑1 (MQ=255)
                                                                      tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                                                 <  2:4378208/150‑1 (MQ=255)
                                                                         aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGGCATCGTGCTGGTGGCCGGGGTGATCCGCCCGCCTCTGACGCCCGTCCTCACTACGCGCCCCGCCCCCa                                                                              >  1:3869796/1‑150 (MQ=255)
                                                                          tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTACGGGAATCGAGCTGCTGGCCGCGGGTATCCGCCCGCCTCTGACGCCCGTACGCACTGCGCGCCCCGcccccca                                                                             >  2:5197473/1‑148 (MQ=255)
                                                                           gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                            <  1:4720566/150‑1 (MQ=255)
                                                                              cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGCCGGGAAGCGAGCTGCTGACCGCTGGTCTCCGCCCGCCTCGGTCGGCGGGGCTCACTACGCGCCCCGCCCCCAAGCac                                                                         >  2:4610031/1‑149 (MQ=255)
                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                        <  1:575084/150‑1 (MQ=255)
                                                                                 tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                      <  1:1180717/150‑1 (MQ=255)
                                                                                   tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGGAATCGCGCTGCGGGCCGCGGGGCTCCGCCCGCAGCGGACGCCAGTGCGCACTCCGCGCCCCGCCCCCAAGCCACggca                                                                    >  1:502988/1‑147 (MQ=255)
                                                                                   tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                    <  1:3106799/150‑1 (MQ=255)
                                                                                       tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                <  1:401348/150‑1 (MQ=255)
                                                                                       tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                <  1:3022914/150‑1 (MQ=255)
                                                                                       tGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                <  2:1506358/150‑1 (MQ=255)
                                                                                        ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                               <  1:2438600/150‑1 (MQ=255)
                                                                                         gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                              <  2:5112472/150‑1 (MQ=255)
                                                                                          aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                             <  1:200820/150‑1 (MQ=255)
                                                                                           ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                            <  1:100515/150‑1 (MQ=255)
                                                                                            tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                           <  2:3182581/150‑1 (MQ=255)
                                                                                            tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                           <  2:248300/150‑1 (MQ=255)
                                                                                              gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑GCCCCCCCGTTCGGTGATCGAGCTGCGGTCCGCGGAGTTCCGCCCGCATCGGTCGCCAGTACTCGCTACGCGCCCCGCCCCCACTCAACGTACCGCTTTGCGGt                                                         >  1:3470945/1‑150 (MQ=255)
                                                                                               ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCCGCATCTGACGCCCGTACTCGCTCCGCGCCCCGCCCCCAGTCAACGTCAGACGTTGCGGtt                                                        >  2:1329248/1‑150 (MQ=255)
                                                                                                cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                       <  2:2781844/150‑1 (MQ=255)
                                                                                                cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                       <  1:2520942/150‑1 (MQ=255)
                                                                                                 gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                      <  1:1450929/150‑1 (MQ=255)
                                                                                                  cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                     <  1:694135/150‑1 (MQ=255)
                                                                                                   gATCTGCGCCAGCCTTGCTGCGCCCCGGGCTGGCCCCCCCT‑CCCCCCCCGTACGGTACTCGAGCTGCAGCCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACAACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                    <  2:3359896/150‑1 (MQ=255)
                                                                                                   gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGTGCGCGGGCTGCTGGCCGCTGTTCTCCGCCAGCATCTGGCGCCGGGTCTCGCTTCGCGCCCCGCCCCCCAGCGACGGAACGCGGTGCGGGTTAAt                                                    >  2:864736/1‑150 (MQ=255)
                                                                                                   gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGGCGGTCCTCGCGCTGCTGTCCGCGGATATCCGCCCGCAGCTGCCGCCAGTCCTCCCTCCGCGCCCCGCCCCCACGCAACGTCACACGTTGCGGTGTAAt                                                    >  2:4861966/1‑150 (MQ=255)
                                                                                                     tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                  <  1:2847561/150‑1 (MQ=255)
                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTCCGGGCATCGCGCTGCGGGCCGCGGTTATCCGCCAGCGTCTGCCGCCAGTGCTCACTGCGCGCCCCGCCCCCCAGCAACGGAACACGTTGCGGTGGAATACCGCAg                                             >  2:1967268/1‑150 (MQ=255)
                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                             <  2:461700/150‑1 (MQ=255)
                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCGGACGCCAGTACGCACTACGCGCCCCGCCCCCAAGCAACGTACCACGTTGCGGGTTAGTACCGCAg                                             >  1:3656951/1‑150 (MQ=255)
                                                                                                           ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                            <  1:4544466/150‑1 (MQ=255)
                                                                                                             aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGCCCGGGGATATCCGCCAGCATCTGACGCCCGGACTCACGACGCGCCCCGCCCCCCATCAACGTGACACTGTGCGGGTTAGTACCGCAGGcc                                          >  2:493460/1‑149 (MQ=255)
                                                                                                                cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTAGGGTAATGGTGCTGGTGACCGCTGTTATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAGCACTGTGCGGTTTTTTACCGGGGGCGTGc                                       >  2:1236755/1‑150 (MQ=255)
                                                                                                                  tgctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                     <  1:2959774/150‑1 (MQ=255)
                                                                                                                   gctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAGACGTTGCGGTTTAATACCGCAGGCATGCACg                                    >  2:1118703/1‑150 (MQ=255)
                                                                                                                   gctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGGATCGAGCTGCTGTCCGCTGAGAGCCGCCCGCCTCGGACGCCCGTACGCACTACGCGCCCCGCCCCCACTCAACGGAACCCGGTGCGGTTGAATACCGCCGGCAGGCCCg                                    >  2:4713568/1‑150 (MQ=255)
                                                                                                                     tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCCCGAc                                  >  2:1734770/1‑150 (MQ=255)
                                                                                                                      gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                 <  2:4416098/150‑1 (MQ=255)
                                                                                                                       cgcCCAGGGCTGGCCCCACATCCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                 <  2:655452/150‑1 (MQ=255)
                                                                                                                        gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACCCGTTGCGGTGTAATACCGCAGGCATGCCCGTCATc                               >  1:903644/1‑150 (MQ=255)
                                                                                                                        gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                               <  1:1028723/150‑1 (MQ=255)
                                                                                                                            aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                           >  2:1769796/1‑150 (MQ=255)
                                                                                                                             gTGCTGGCCCCACATCCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCGGATATCCGCCAGCAACAGACGCCAGTACACACTACGCGCCCCGCCCCCAAACAACGGAACACTTTTCGGTTTATTACCGCAGGCATGCACGACATCCATg                           >  1:1675732/1‑150 (MQ=255)
                                                                                                                             gTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                          >  2:5009109/1‑150 (MQ=255)
                                                                                                                             gTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAAGCACGACATCCATGc                          >  1:4311751/1‑150 (MQ=255)
                                                                                                                              tGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc                         >  2:3543680/1‑150 (MQ=255)
                                                                                                                              tGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc                         >  1:2153127/1‑150 (MQ=255)
                                                                                                                               gCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                        <  1:4182670/150‑1 (MQ=255)
                                                                                                                                cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACGTTGCGGTGTAATACCGCAGGCATGCACGACATCCCTGCCAt                       >  2:1433696/1‑150 (MQ=255)
                                                                                                                                  ggCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACACACGACGCGCCCCGCCCCCAATCAAAGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                     >  1:1323438/1‑150 (MQ=255)
                                                                                                                                      ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAACCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                 >  1:4741376/1‑150 (MQ=255)
                                                                                                                                       cacaT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg                >  2:3825616/1‑150 (MQ=255)
                                                                                                                                         caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg              >  2:2950981/1‑150 (MQ=255)
                                                                                                                                               ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa         >  1:1875682/1‑150 (MQ=255)
                                                                                                                                               ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa         >  1:280343/1‑150 (MQ=255)
                                                                                                                                                cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc        <  1:4115211/150‑1 (MQ=255)
                                                                                                                                                  cccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc      >  1:3151290/1‑150 (MQ=255)
                                                                                                                                                    cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAc    >  2:2712152/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  CP009273/3721331‑3721629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑