BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,345	T→G	9.3%	V14G (GTT→GGT)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,345	0	T	G	9.3%	553.6 / 3.2	240	V14G (GTT→GGT)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base T (132/76); new base G (26/3); total (161/79)
Fisher's exact test for biased strand distribution p-value = 5.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

AAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTA  >  CP009273/3822196‑3822493

aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                      <  2:4337437/150‑1 (MQ=255)

  aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGttt                                                                                                                                                    >  1:1355694/1‑149 (MQ=255)

   aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                                                                                                                                   <  1:3219811/150‑1 (MQ=255)

   aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                                                                                                                                   <  2:4777816/150‑1 (MQ=255)

    aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgcg                                                                                                                                                  >  1:2505421/1‑150 (MQ=255)

     gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACAGGTTgcgc                                                                                                                                                 >  2:2647791/1‑150 (MQ=255)

          tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                                                                                                                            >  2:5024863/1‑150 (MQ=255)

             aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTTCGCAGAcaaaa                                                                                                                                         >  1:85696/1‑146 (MQ=255)

                tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                      >  2:3766546/1‑150 (MQ=255)

                tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTAGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCCGTGCGCACACACaaaaa                                                                                                                                      >  1:3506462/1‑149 (MQ=255)

                  aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                                    >  2:2612523/1‑150 (MQ=255)

                   tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCCATGCGCACACACaaaaaacc                                                                                                                                   >  1:4136354/1‑146 (MQ=255)

                   tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTTCGCCGACACAAAACaac                                                                                                                                   >  1:808317/1‑148 (MQ=255)

                     tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                                 >  2:3360726/1‑150 (MQ=255)

                     tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCAACCCTCGAGTCAGAAAATGGGCAACCGGTTGCGCAGACTAAAAACAgagt                                                                                                                                 >  2:4192778/1‑147 (MQ=255)

                       aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAAc                                                                                                                               >  2:1217691/1‑150 (MQ=255)

                        atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGCGTGCGCCCACACAAAAAACCGAAca                                                                                                                              >  1:5070620/1‑149 (MQ=255)

                        atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCACACACAAAAAAGCGAACt                                                                                                                              >  1:198449/1‑150 (MQ=255)

                          atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGCCTGCCCCGACTCAAAACACCGaccaga                                                                                                                            >  1:4204571/1‑145 (MQ=255)

                          atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                            >  2:5107280/1‑150 (MQ=255)

                             tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                         >  2:3150348/1‑150 (MQ=255)

                              aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                        >  2:1791167/1‑150 (MQ=255)

                              aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTa                                                                                                                        >  2:1803252/1‑150 (MQ=255)

                               gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                       >  2:1957983/1‑150 (MQ=255)

                               gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACTGATTTaa                                                                                                                       >  1:4692023/1‑149 (MQ=255)

                                aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACAAAAAACAACCAACAGAATTaac                                                                                                                      >  1:3227105/1‑148 (MQ=255)

                                aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCACACACAAAAAAACGAACAGATTTaac                                                                                                                      >  1:2383274/1‑148 (MQ=255)

                                  gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAACCAACAGATTTAccct                                                                                                                    >  1:3165132/1‑148 (MQ=255)

                                   aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                                   >  2:2606184/1‑150 (MQ=255)

                                   aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                                   >  2:2139822/1‑150 (MQ=255)

                                      aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTg                                                                                                                >  2:2267046/1‑150 (MQ=255)

                                       ggtgGCAATGTTTTGCTTTTCTCTTTCGGTCAGCTAAATTGCCCGCTTTGCTACCTGGGGTTTTTTTTCTTTGTCTGTTTCCACCCTCGCGGCATAAAATGCGCACCCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                               <  1:5202182/149‑1 (MQ=255)

                                           gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCCAACAGATTTACCGTCTTGAAGAt                                                                                                           >  2:1734812/1‑150 (MQ=255)

                                              aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                        >  2:217952/1‑150 (MQ=255)

                                               aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAACGAACAGATTTAAAGTCTTGTAGATAGta                                                                                                       >  1:1544684/1‑149 (MQ=255)

                                                  tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCAAAAAAGCGAACAGTTTTTCCCTCTTGAAGATAGTacgc                                                                                                    >  1:1785028/1‑150 (MQ=255)

                                                   ttGCTTTTCTCTTTCGGTCAGCTACAATGCCCGCTTGGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgcc                                                                                                   <  2:4861181/150‑1 (MQ=255)

                                                   ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACGCAAAACAGCGAACGGATTTACCGTCTTGAAGATCGTccgcc                                                                                                   >  1:4367260/1‑150 (MQ=255)

                                                   ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCCACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGCCGCAAAACAGCCAACAGAATTAACGACATGAAAATACTccgcc                                                                                                   >  1:3110853/1‑150 (MQ=255)

                                                    tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTATCTCGTgcgccg                                                                                                  >  2:3953997/1‑150 (MQ=255)

                                                       ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTGAAGATCGTCCGCCGCtt                                                                                               >  2:874018/1‑150 (MQ=255)

                                                         ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                             >  1:3402272/1‑150 (MQ=255)

                                                            tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                          >  2:5186604/1‑150 (MQ=255)

                                                            tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                          >  2:4920514/1‑150 (MQ=255)

                                                            tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                          >  2:4921549/1‑150 (MQ=255)

                                                            tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                          >  2:4805119/1‑150 (MQ=255)

                                                               ttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCCGTCTAAAAAAAGCGAACTGCTTTACCGTCTTTTAGATCGTCCGGCGCGTCCACAAAc                                                                                       >  2:2648453/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACACAAAAAAGCGAACAGATTTACCCGCGTGAAGATCGTCCGCCCCTGCCACCAAcc                                                                                      >  1:4515236/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                      >  2:2932643/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                      >  2:2576295/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGGCCTCAAAcc                                                                                      >  2:156804/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGGCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATGTCGTCCGCCGCTGCCTCAAAcc                                                                                      >  2:3562093/1‑150 (MQ=255)

                                                                     cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                                 >  2:4533559/1‑150 (MQ=255)

                                                                         tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcc                                                                             <  2:5172783/150‑1 (MQ=255)

                                                                          aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCGACCCCGTGGGCAGACTCAAAAAAAAGAACAGATTTAACGTCTGTAAGATAGTCCGCCGCGTCCACAAACCCAGGTTtccg                                                                            >  1:1330271/1‑150 (MQ=255)

                                                                            aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcagcc                                                                          >  2:4060806/1‑150 (MQ=255)

                                                                             aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCt                                                                         >  2:5239927/1‑150 (MQ=255)

                                                                              tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTg                                                                        <  2:2539268/150‑1 (MQ=255)

                                                                               gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCCGGTGCGGAGACTCAAAAAAGCGAACAGATTTTACGTCGTTTAGATAGTCCGCCGCGTCCTCAAACCCAGTTTTGCGCCTGt                                                                       >  1:987837/1‑150 (MQ=255)

                                                                                 ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACACAAAACACCCAACAGATGTACCCGCGTGAAGAACGTCCGCCGCATCCTCCAACCCACGTTGCCGCCGGTCa                                                                     >  1:2317678/1‑150 (MQ=255)

                                                                                  ctcTTTGCTACCTCGGTTTTGTTTTGGTTTTCGGTTACCACCCTCGAGCCATAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                    <  1:2063502/148‑1 (MQ=255)

                                                                                  cGCTTTGCTCCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                    <  2:2208064/150‑1 (MQ=255)

                                                                                  cGCTTTGCTACCTGGGGTTTTTTTTGTTTGTCTTGTCCCACCCGCGCGCCATAAACTGCGCCCCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                    <  1:2455611/150‑1 (MQ=255)

                                                                                    ctgtGCTACCTGGGGTTTGTTTTCGATGTCTGTGACCCCCCTCGCGTCAGAAATGGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                                  <  1:1721006/147‑1 (MQ=255)

                                                                                       tGCTACCAGGGGTTTGTTTTGGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                               <  2:2211877/150‑1 (MQ=255)

                                                                                          tACCACGGTATTTTTTTCGTTGTCTTTTTCCTCCCTCGAGTCAGAAATTGCGCAAGCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGACAGCATctgct                                                            <  2:3931696/150‑1 (MQ=255)

                                                                                          tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgct                                                            <  2:5157265/150‑1 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCCCCCGTTGGGCACACACAAAACAACCAACAGAATTAACGAAGTGAAGATACTCCGCCGCAGCCACAAACCCACGTTTCAGCCAGTCAGCAAcagctg                                                           >  1:1005327/1‑150 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTTCGCAGACGCAAAAAACACAACAGATATAACCTATTTAATATCGTCCCCCCCATCCACAAACCCAGTTTTCCGCCAGTCACCaacagcag                                                           >  1:4593644/1‑143 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAAAGCGAACAGATTTAACCTCTTGTAGATAGTCCGCCGCTTCCACAAACCCGGTTTTCCGCCGGTCAGCAActgctg                                                           >  1:196361/1‑150 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACAAAAAAAACCGAAAATATTTAACGTCTTTAAGATCGGCCGCCCCGTCCACAAAACCCGTTTTACGCCAGTCAGCATctgctg                                                           >  1:1488436/1‑150 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                           <  2:1032365/150‑1 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGAGGTCTGTTTCCACCCACGAGTCACAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                           <  2:146388/150‑1 (MQ=255)

                                                                                            ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCCCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                          >  2:1877887/1‑150 (MQ=255)

                                                                                             ccggggTTTGTTTTCGTTGTCGTTTACCACCCTCGAGCAATAAATGGCGCACCGGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                         <  1:2908501/147‑1 (MQ=255)

                                                                                             cACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCCAAAAAGCCAACAGATTTACCGTCGTGAAGATAGGCCGCCCCTGCCACAAAACCAGTTTTCCGCCAGTCAGCACCTGCGGGc                                                         >  1:1852957/1‑150 (MQ=255)

                                                                                                gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                      <  2:4626764/150‑1 (MQ=255)

                                                                                                         ttCTATGTCTGTTTCCCCCCTCGAGTCTTAAATGGCGAACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                             <  1:2177945/150‑1 (MQ=255)

                                                                                                           cGATGTCTGTTACCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                           <  1:3470370/150‑1 (MQ=255)

                                                                                                             ttGTCTGTGGCCCCCCTCGAGTCAGAAACTGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGCTTGACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                         <  2:4136354/149‑1 (MQ=255)

                                                                                                             aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGAAACAAAAAAACGAACGTATTTAACGTCATGAAGATCGTCCGCCCCGTCCTAAAAACCAGTTTTCCGCCAGTAAGCATCATCTTGCGATGTTCGTTGCGGTg                                         >  1:4923055/1‑150 (MQ=255)

                                                                                                                  tGTTACCCCCCTCGAGAAATAAAATGCGAAACGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                    <  1:1118359/150‑1 (MQ=255)

                                                                                                                        cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACTCAAACCCGGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCCgc                              >  2:3736245/1‑150 (MQ=255)

                                                                                                                        cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                              >  2:4645908/1‑150 (MQ=255)

                                                                                                                           ccTCGAGTCAGAAAATGCTCAAGCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt                           <  2:5087640/150‑1 (MQ=255)

                                                                                                                                  tCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCCAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCAGTCAGCATCTGCTGGCGCTGGTCGTTGCGGTGATCACGCCCGCGCTAATAATc                    >  2:4484954/1‑150 (MQ=255)

                                                                                                                                  tCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                    >  2:327277/1‑150 (MQ=255)

                                                                                                                                   cAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt                   <  2:2756059/150‑1 (MQ=255)

                                                                                                                                   cAGAAAATGCGCAACCGGTGGCGCAGACTCAACACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt                   <  2:131152/150‑1 (MQ=255)

                                                                                                                                    aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTTAAGATAGTCCGCCGCTTCCACAAACCCGGGTTTCCGCCAGTCAGCATATGCTGGCGATGTTAGTTTCGGTGATCACGCCAGCGCTATTAAtatt                  >  1:4772437/1‑147 (MQ=255)

                                                                                                                                    aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg                  <  2:2990739/150‑1 (MQ=255)

                                                                                                                                       aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa               >  2:831793/1‑150 (MQ=255)

                                                                                                                                         tggcgcACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg             <  1:2777810/148‑1 (MQ=255)

                                                                                                                                             gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt         >  1:743974/1‑150 (MQ=255)

                                                                                                                                             ccccccGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt         <  1:2427875/146‑1 (MQ=255)

                                                                                                                                               accgggTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg       <  1:3775362/146‑1 (MQ=255)

                                                                                                                                               acccGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg       <  1:1144614/148‑1 (MQ=255)

                                                                                                                                               aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGTGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg       >  1:3792136/1‑150 (MQ=255)

                                                                                                                                                  cGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGtt    <  2:3394936/150‑1 (MQ=255)

                                                                                                                                                   ggTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGttt   >  2:3875206/1‑150 (MQ=255)

AAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTA  >  CP009273/3822196‑3822493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`