Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,511 A→C 5.3% V323V (GTT→GTG)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,5110AC 5.3% 1013.0 / 4.8 355V323V (GTT→GTG) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base A (192/132);  new base C (2/29);  total (194/161)
Fisher's exact test for biased strand distribution p-value = 6.42e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

CCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGG  >  CP009273/3872362‑3872660
                                                                                                                                                     |                                                                                                                                                     
  aaCCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAAc                                                                                                                                                     <  2:1056282/150‑1 (MQ=255)
   aCCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACg                                                                                                                                                    >  1:2011327/1‑150 (MQ=255)
    ccACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGa                                                                                                                                                   >  1:4801246/1‑150 (MQ=255)
     cACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGAc                                                                                                                                                  <  2:655222/150‑1 (MQ=255)
     cACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTAATCTGCTGTTCAACCGCCGATTTCAACTCAGAAGAAAACAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGAc                                                                                                                                                  >  1:1890812/1‑150 (MQ=255)
      aCGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTTGTCTGGGAGGAGAATTTCGGGTCCGGCACATTCACGGAAACGAcc                                                                                                                                                 >  1:1357434/1‑150 (MQ=255)
            ttCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATc                                                                                                                                           >  2:1985719/1‑150 (MQ=255)
                cgtcgtTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCAACTGATGTTAAACCGCCGATTTAACCTCAGAAGAAACCAGTTTTTCTTTTGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCCCAATCatga                                                                                                                                       >  2:5127133/1‑147 (MQ=255)
                  tCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCt                                                                                                                                     >  1:1013483/1‑150 (MQ=255)
                   cGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGAGATTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCtt                                                                                                                                    <  2:1714221/150‑1 (MQ=255)
                     gTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCa                                                                                                                                  >  1:1709923/1‑150 (MQ=255)
                      ttGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCAc                                                                                                                                 >  1:970889/1‑150 (MQ=255)
                       tGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCAcg                                                                                                                                >  2:2907650/1‑150 (MQ=255)
                        gggTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCAcgc                                                                                                                               >  1:3628798/1‑150 (MQ=255)
                             ttCCCGCAGTTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGTAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATc                                                                                                                          <  2:2495166/150‑1 (MQ=255)
                             ttCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATc                                                                                                                          >  2:3232442/1‑150 (MQ=255)
                                 agctgGTATTCTGCCTGATTTTCGTTCTTCTGCTTTACAACCGCCGATTTCACCTCAGAATAAACCTTTTTGTCTTTGGTCTGGGAGTTTATTTTGGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCa                                                                                                                      <  1:742216/150‑1 (MQ=255)
                                 agcagGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCa                                                                                                                      >  2:3977490/1‑150 (MQ=255)
                                   cagtttttCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCTATTTCACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGTGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCAcc                                                                                                                    <  2:3164490/144‑1 (MQ=255)
                                     ggTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCgg                                                                                                                  >  2:5108739/1‑150 (MQ=255)
                                       ttttCTGCCAGCAGTTCTTTCATCTGCTGTACACCCGCCGATTTCACCTCAGAACAACCCAGTTTGTCTTTGGTGGGGGTGGATATTTTGGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTg                                                                                                                <  2:3878/148‑1 (MQ=255)
                                       tATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTg                                                                                                                >  1:5106987/1‑150 (MQ=255)
                                        aTTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTgg                                                                                                               >  1:2615353/1‑150 (MQ=255)
                                        aTTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTgg                                                                                                               >  1:5240990/1‑150 (MQ=255)
                                             gCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTg                                                                                                          >  2:2651044/1‑150 (MQ=255)
                                                agcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACt                                                                                                       >  1:1469844/1‑150 (MQ=255)
                                                 gcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAATAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACtt                                                                                                      <  2:2460867/150‑1 (MQ=255)
                                                 gcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACtt                                                                                                      >  1:885456/1‑150 (MQ=255)
                                                  cagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACttt                                                                                                     <  2:714175/150‑1 (MQ=255)
                                                   agTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTg                                                                                                    >  2:3357227/1‑150 (MQ=255)
                                                    gttggttcATCTGCTTTTCAACCGCCGATTTCACCTCAGAATAAACCAGTTTGTCTTTGGTCTGGGAGTATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTgg                                                                                                   <  2:1809334/150‑1 (MQ=255)
                                                    gtgcgttcAGCTGCTGTTCAACCGCCTATTTCACCTCACAAGAAACCAGTTTGTCTTTGGTCTGGTAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTgg                                                                                                   <  2:1808933/147‑1 (MQ=255)
                                                      tcgttcATCTGCTGTACAACCGCCGATTTCACCACATAAGAAACCTGTTTGTCTTTGGTCTGGGAGGATATTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCt                                                                                                 <  1:1279124/150‑1 (MQ=255)
                                                        gttcATGTGCTTTTCAACCGCCTTTTACACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGTGGATATTTTGGGGTCCGGCAATTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCttt                                                                                               <  1:1143012/150‑1 (MQ=255)
                                                        gttcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCttt                                                                                               >  2:4350735/1‑150 (MQ=255)
                                                        gttcAGCTGCTGTGCAACCGCCGATTCCACCTCAGAAGAAACCAGTGGGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTGCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCttt                                                                                               <  2:4399660/150‑1 (MQ=255)
                                                         ttcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtttt                                                                                              >  2:3763142/1‑150 (MQ=255)
                                                           cctCTGCTTTACAACCGCCGATTTCACCTAAGACCAAACCTTTTTGTATTTGGTCTGGGTGGATATTTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtttttt                                                                                            <  1:4953071/148‑1 (MQ=255)
                                                           cATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtttttt                                                                                            >  2:4540586/1‑150 (MQ=255)
                                                           cATCGGCTGTCCACCCGCCGATTACACCTCACAAGAAACCTGTTTTTTTTTGGTCGGGGTGTATATTTTGGGGTCCGCCACTTTCCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtttttt                                                                                            <  1:2219737/150‑1 (MQ=255)
                                                            aTCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTg                                                                                           >  2:1596442/1‑150 (MQ=255)
                                                             tCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGc                                                                                          >  1:3061062/1‑150 (MQ=255)
                                                              ctgctgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCt                                                                                         >  1:241209/1‑150 (MQ=255)
                                                                      cAACCGCCGATTTCACCTCCGACGAACCCAGTTGGTCTTTGGTCTGGGTGGAGATTTTCGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCctt                                                                                 <  2:3265996/150‑1 (MQ=255)
                                                                      cAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCctt                                                                                 >  1:1436111/1‑150 (MQ=255)
                                                                       aaCCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGATTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttc                                                                                <  2:1557696/150‑1 (MQ=255)
                                                                       aaCCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttc                                                                                >  2:3481127/1‑150 (MQ=255)
                                                                        cccgccgATTTCACCTCAGCAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTGGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttct                                                                               <  2:4377576/149‑1 (MQ=255)
                                                                          cgcctATTTCACCTCAGAAGAAACCCTTTTGTGTTTGTTGTGGGAGGATATTTTCGGGTCGGCCAATTTCCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                             <  1:4094121/150‑1 (MQ=255)
                                                                          cgccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                             >  2:4960610/1‑150 (MQ=255)
                                                                           gcctATTTCACCTCAGAACAAACCAGTTTGTCTTTGGTCTGGGAGCAGATTTTCGGGACCGGCACTTACCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTg                                                                            <  1:1830489/150‑1 (MQ=255)
                                                                            ccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGt                                                                           >  2:606774/1‑150 (MQ=255)
                                                                              gATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTcc                                                                         >  2:3310529/1‑150 (MQ=255)
                                                                              gATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTGTGTcc                                                                         >  2:3085723/1‑150 (MQ=255)
                                                                              gATTTCACCACAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGATAATTTCGGGTCCGGCAATTTCACGTACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTcc                                                                         <  1:4231527/150‑1 (MQ=255)
                                                                               aTTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCa                                                                        >  2:3122502/1‑150 (MQ=255)
                                                                               aTTTCACCTCAGAAGAAACCAGTTTGTCTGTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCa                                                                        >  2:3570802/1‑150 (MQ=255)
                                                                                tttCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCAt                                                                       >  1:798174/1‑150 (MQ=255)
                                                                                 ttCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATg                                                                      <  2:3335529/150‑1 (MQ=255)
                                                                                 tgcACCTCAGAAGAACCCAGTTTGTCTTTGGTCTGGGAGTATAATTTCGGGTCCGGCAATTCAACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATg                                                                      <  1:2968630/148‑1 (MQ=255)
                                                                                  tCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGt                                                                     >  1:3673749/1‑150 (MQ=255)
                                                                                   cACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                    <  2:3733769/150‑1 (MQ=255)
                                                                                   cACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                    >  1:2855450/1‑150 (MQ=255)
                                                                                   cACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                    >  1:5177259/1‑150 (MQ=255)
                                                                                   cACCTAAGAATAAACCTGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                    <  1:3840615/150‑1 (MQ=255)
                                                                                          gaagaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTc                                                             >  2:4795619/1‑150 (MQ=255)
                                                                                            agaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAg                                                           >  1:3215237/1‑150 (MQ=255)
                                                                                            agaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAg                                                           <  2:5239799/150‑1 (MQ=255)
                                                                                              aaaCCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAggg                                                         >  1:4269061/1‑150 (MQ=255)
                                                                                              aaaCCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAggg                                                         >  2:3923801/1‑150 (MQ=255)
                                                                                                 ccAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTATCCTTCTTTGTCCATGTAGGCGTTCAGGGTAc                                                      >  1:466606/1‑150 (MQ=255)
                                                                                                 ccAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTAc                                                      >  2:4704558/1‑150 (MQ=255)
                                                                                                  cAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACg                                                     >  2:2432979/1‑150 (MQ=255)
                                                                                                     tttGTATTTGGTCTGGGAGTTGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGt                                                  <  2:1342296/150‑1 (MQ=255)
                                                                                                      ttGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTc                                                 >  1:3981017/1‑150 (MQ=255)
                                                                                                            tgggTCTGGGAGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATcgcc                                           <  2:2855692/148‑1 (MQ=255)
                                                                                                             tGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAATGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATcgccg                                          >  2:2220617/1‑150 (MQ=255)
                                                                                                                     gaggagAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAg                                  >  1:674019/1‑150 (MQ=255)
                                                                                                                       ggagAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGcc                                <  2:3816353/150‑1 (MQ=255)
                                                                                                                       ggagAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGcc                                >  1:4531953/1‑150 (MQ=255)
                                                                                                                        gagaTTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCt                               <  2:4879884/150‑1 (MQ=255)
                                                                                                                        gagaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGACGCCt                               >  2:1646052/1‑150 (MQ=255)
                                                                                                                         agaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                              >  2:3054872/1‑150 (MQ=255)
                                                                                                                         agaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                              >  1:5082432/1‑150 (MQ=255)
                                                                                                                             tttCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAg                          <  1:3252824/150‑1 (MQ=255)
                                                                                                                              ttCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAgg                         <  1:2770126/150‑1 (MQ=255)
                                                                                                                                cGGGTCCGGCACTGTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTg                       <  2:3649216/150‑1 (MQ=255)
                                                                                                                                 gggTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGa                      <  2:1202804/150‑1 (MQ=255)
                                                                                                                                     ccGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAc                  >  2:1152666/1‑150 (MQ=255)
                                                                                                                                      tggCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAcc                 >  1:2301850/2‑150 (MQ=255)
                                                                                                                                      cGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAcc                 <  2:2785660/150‑1 (MQ=255)
                                                                                                                                        gCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccgc               >  1:5211361/1‑150 (MQ=255)
                                                                                                                                        gCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccgc               >  2:864613/1‑150 (MQ=255)
                                                                                                                                         cACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccgcc              >  2:2368894/1‑150 (MQ=255)
                                                                                                                                          aCTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccgccg             >  1:1578019/1‑150 (MQ=255)
                                                                                                                                            tttCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTc           <  2:1359283/150‑1 (MQ=255)
                                                                                                                                              tCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGGCAc         >  2:2203423/1‑150 (MQ=255)
                                                                                                                                               cACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACg        >  2:4168731/1‑150 (MQ=255)
                                                                                                                                                  gtaaaCGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCta     <  1:3758628/148‑2 (MQ=255)
                                                                                                                                                     aaCGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCgg  <  2:1890225/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGG  >  CP009273/3872362‑3872660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑