Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,085,145 A→C 8.3% A194A (GCT→GCG)  frvB ← putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,085,1450AC 8.3% 1230.3 / 21.0 441A194A (GCT→GCG) frvBputative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (228/162);  new base C (3/47);  total (231/210)
Fisher's exact test for biased strand distribution p-value = 1.59e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 446 total aligned reads displayed.

ACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGC  >  CP009273/4084996‑4085293
                                                                                                                                                     |                                                                                                                                                    
aCGAAATAGCCAATCGCCAGCCCCAGCCCCACCGCGCCGAGGAATCCCGCCCCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATaa                                                                                                                                                      <  1:3733944/150‑1 (MQ=255)
 cGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAg                                                                                                                                                     >  1:1506366/1‑150 (MQ=255)
      tAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGccc                                                                                                                                                >  1:1820153/1‑150 (MQ=255)
        gCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCat                                                                                                                                              >  1:2135066/1‑150 (MQ=255)
          cAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCatga                                                                                                                                            >  1:435123/1‑150 (MQ=255)
          cAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCatga                                                                                                                                            >  1:286619/1‑150 (MQ=255)
          cAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCatga                                                                                                                                            >  2:1988553/1‑150 (MQ=255)
           aaTCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCatgat                                                                                                                                           >  2:4688002/1‑150 (MQ=255)
           aaTCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCatgat                                                                                                                                           >  1:1439608/1‑150 (MQ=255)
            aTCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGAACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCAAGATAGCAAACCAGAAACGCCGTAGCGAAAGCCGGTTTATCGGAAATCGACGAGGCAATATAAGCGCCCatgatg                                                                                                                                          <  2:2459797/150‑1 (MQ=255)
             tCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAAGATAAGCGCCCATGATgg                                                                                                                                         >  2:2815484/1‑150 (MQ=255)
              cGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATggg                                                                                                                                        >  2:621044/1‑150 (MQ=255)
               gCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCACTCGACGAGGCCATATCAGCGCCCAGGATgggg                                                                                                                                       >  2:4164125/1‑150 (MQ=255)
                 cAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGAt                                                                                                                                     <  2:4462483/150‑1 (MQ=255)
                 cAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGAt                                                                                                                                     >  1:309656/1‑150 (MQ=255)
                 cAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGAt                                                                                                                                     >  2:1637184/1‑150 (MQ=255)
                  aGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATc                                                                                                                                    >  1:1942911/1‑150 (MQ=255)
                    ccccAGCACCACCGCGCCGTGTAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCAt                                                                                                                                  <  2:4113013/150‑1 (MQ=255)
                      cccgCACCCCCGCGCCGAGTAACCCCGCCCCCGACTGGGTGCCGAGCAGCGATTGGGCGTTCGCCAGATAGCAAACCAGAACCGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATaa                                                                                                                                <  1:2020081/147‑1 (MQ=255)
                       cAGCACCACCGCGCCGAGGAATCCCGCCCCCGACTGGGTGCCGAGAAGCGATTTGTCTTTCGCCAGATCGCAAACCAGAAACGCCGGAGCGAATGCCGGTTTATCGGAAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATaaa                                                                                                                               <  1:1729723/150‑1 (MQ=255)
                       cAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATaaa                                                                                                                               >  1:4765139/1‑150 (MQ=255)
                        accaccccCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGATTTTTCGTTCGCCATATACAAAACCACAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAg                                                                                                                              <  2:1790224/148‑1 (MQ=255)
                        aGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAg                                                                                                                              >  1:2857231/1‑150 (MQ=255)
                          caccacCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGt                                                                                                                            <  2:1259475/150‑1 (MQ=255)
                           accaccGCGCCGTGTAATCCCGCACCCGAGTGGGTGCCGAGCAGCGGTTTGTGGTTCGCCAGATAGCAAACCCCAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTg                                                                                                                           <  1:3275070/150‑1 (MQ=255)
                           accaccGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTg                                                                                                                           >  1:2738898/1‑150 (MQ=255)
                                cgcgCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGcc                                                                                                                      >  2:4608469/1‑150 (MQ=255)
                                cgcgCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGATTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGcc                                                                                                                      >  2:4724174/1‑150 (MQ=255)
                                 gcgcCGAGGAATCCCGCACCCGACTGGGTGCCGAGCCGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCa                                                                                                                     <  2:2319462/150‑1 (MQ=255)
                                   gcCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAg                                                                                                                   >  1:3033636/1‑150 (MQ=255)
                                   gcCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAg                                                                                                                   >  2:376003/1‑150 (MQ=255)
                                      gAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGAAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTa                                                                                                                <  1:2112999/150‑1 (MQ=255)
                                       aGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTaa                                                                                                               >  1:3761671/1‑150 (MQ=255)
                                         taagcccGCACCCGAGTGGGTGCCTAGCTGGGTTTTGTCGTTCGCCATAAACCAACCCCCAAACGCCGGTGCTAACGCCGGTTTATCGGCAATCGACGTGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAAcc                                                                                                             <  1:4933806/146‑1 (MQ=255)
                                                   cccGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCa                                                                                                   <  1:1238836/150‑1 (MQ=255)
                                                           ggTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCa                                                                                           >  2:1494494/1‑150 (MQ=255)
                                                             tGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATTAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAgg                                                                                         <  2:3409984/150‑1 (MQ=255)
                                                              gCCGAGCAGCGTTTTGTCGTTCGCCTTATAGCAAACCAGAAACGCCGGAGCGAAAGCCGTTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGt                                                                                        <  1:5076879/150‑1 (MQ=255)
                                                                cGAGCAGCGTTTTGTCTTTCGCCATATACAAAACAACAAACGCCGGAGCTAAAGCCGTTTTATCGGAAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTaa                                                                                      <  1:4574680/150‑1 (MQ=255)
                                                                cGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCATAAACGCCGGTGCTAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTaa                                                                                      <  2:3154562/150‑1 (MQ=255)
                                                                cGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTaa                                                                                      >  1:3824031/1‑150 (MQ=255)
                                                                cGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTaa                                                                                      <  2:1671532/150‑1 (MQ=255)
                                                                cGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTaa                                                                                      <  2:2582834/150‑1 (MQ=255)
                                                                 gAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTaaa                                                                                     >  1:3213209/1‑150 (MQ=255)
                                                                  agcagcGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAac                                                                                    >  2:2176372/1‑150 (MQ=255)
                                                                  agcagcGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAac                                                                                    >  2:4803442/1‑150 (MQ=255)
                                                                     agcGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTCCCCAGGTAAacgac                                                                                 >  2:3992497/1‑150 (MQ=255)
                                                                     agcGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAacgac                                                                                 >  1:4252399/1‑150 (MQ=255)
                                                                     agcGATTGGTGGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGAAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAacgac                                                                                 <  2:2229626/150‑1 (MQ=255)
                                                                      gcgcTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAacgacg                                                                                <  2:3486183/150‑1 (MQ=255)
                                                                                  tCGCCATATACAAAACCACAAACGCCGGAGCTAATGCGTTTTTATGGGAAATCGACGAGGAAATATACGCGCCATTGTTGGGGATAATAATGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGTTAAACGACGGCGCGCCTTTCg                                                                    <  1:702743/150‑1 (MQ=255)
                                                                                  tCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCg                                                                    >  2:365139/1‑150 (MQ=255)
                                                                                   cGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGa                                                                   >  2:5178663/1‑150 (MQ=255)
                                                                                       aGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACata                                                               >  2:2011048/1‑150 (MQ=255)
                                                                                          tAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCg                                                            >  2:1562199/1‑150 (MQ=255)
                                                                                          tAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCg                                                            >  2:5146388/1‑150 (MQ=255)
                                                                                           aGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCgg                                                           >  2:1179112/1‑150 (MQ=255)
                                                                                            gCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGt                                                          >  1:4053593/1‑150 (MQ=255)
                                                                                             aaaaCCAGAACCGCCGGAGCTAACGCCGGTTTATCGGCAATCGACGAGGAAATATACGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGta                                                         <  2:3251617/149‑1 (MQ=255)
                                                                                              aaaCCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGtat                                                        >  2:2412597/1‑150 (MQ=255)
                                                                                               aaCCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGtata                                                       >  1:921207/1‑150 (MQ=255)
                                                                                                aCCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAACGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATAc                                                      <  2:3296676/150‑1 (MQ=255)
                                                                                                 ccACAACCGCGGTAGCTAACGCGGTTTTATCGGAAATCGACTCGGAAATATAAGCGCCCATGATGGGTATCATAAAGGTGAAGCCAAGGTAACCGATGTATTAAACTACCCTGTTAAACGACGGCGCGCCTTTCGACATATCGGTATACg                                                     <  1:1160334/150‑1 (MQ=255)
                                                                                                    gAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCa                                                  >  2:3786125/1‑150 (MQ=255)
                                                                                                     aaaCGCCGGAGCTAAAGCGGGTTTATCGGCAATCGACGAGGCAATATACGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCaa                                                 <  1:392680/150‑1 (MQ=255)
                                                                                                      aaCGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCaaa                                                >  2:1769420/1‑150 (MQ=255)
                                                                                                      aaCGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCaaa                                                >  2:4924242/1‑150 (MQ=255)
                                                                                                         gCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCg                                             >  1:775072/1‑150 (MQ=255)
                                                                                                          ccGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGa                                            >  1:1086774/1‑150 (MQ=255)
                                                                                                            ggAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAAc                                          >  2:725569/1‑150 (MQ=255)
                                                                                                            ggAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAAc                                          >  2:1078374/1‑150 (MQ=255)
                                                                                                            ggAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAAc                                          >  1:5052160/1‑150 (MQ=255)
                                                                                                              aGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATTATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTg                                        <  2:5010966/150‑1 (MQ=255)
                                                                                                               gCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGc                                       >  2:4125915/1‑150 (MQ=255)
                                                                                                                 gAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCAc                                     >  1:4425102/1‑150 (MQ=255)
                                                                                                                  aaaGCGGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCAcc                                    <  2:2935697/150‑1 (MQ=255)
                                                                                                                  aaaGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCAcc                                    >  2:936747/1‑150 (MQ=255)
                                                                                                                  aaaGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCAcc                                    >  1:1109161/1‑150 (MQ=255)
                                                                                                                     gCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGc                                 <  2:435123/150‑1 (MQ=255)
                                                                                                                        ggTTTATCGGAAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGAGGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATg                              <  1:2877248/150‑1 (MQ=255)
                                                                                                                          tttATCGGCAATCGACGAGGCAATATACGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGtt                            <  2:2063692/150‑1 (MQ=255)
                                                                                                                           ttATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTg                           >  2:807210/1‑150 (MQ=255)
                                                                                                                            tATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTgg                          >  1:4128027/1‑150 (MQ=255)
                                                                                                                               cGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCaa                       >  2:1136873/1‑150 (MQ=255)
                                                                                                                                  cAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAgcg                    >  2:1081247/1‑150 (MQ=255)
                                                                                                                                    aTCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGcc                  >  1:561074/1‑150 (MQ=255)
                                                                                                                                     tCGACTAGGCAATATACGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGcca                 <  2:3556965/150‑1 (MQ=255)
                                                                                                                                     tCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGcca                 >  1:2462743/1‑150 (MQ=255)
                                                                                                                                      cgactaGGAAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGccac                <  1:4195415/150‑1 (MQ=255)
                                                                                                                                      cgacgtGGCAATATACGCGCCCATGATGGGTATCTTAAAGGTGAAGCCAGTGTAACCGATGTATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGccac                <  1:3425165/150‑1 (MQ=255)
                                                                                                                                      cgacgaGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGccac                >  1:2728821/1‑150 (MQ=255)
                                                                                                                                      cgacgaGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGccac                >  1:2306268/1‑150 (MQ=255)
                                                                                                                                       gacgaGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGccacc               >  2:4177157/1‑150 (MQ=255)
                                                                                                                                            ggCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGAt          >  1:3365020/1‑150 (MQ=255)
                                                                                                                                             gCAATATACGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATa         <  2:1010379/150‑1 (MQ=255)
                                                                                                                                             gCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATa         >  1:2603462/1‑150 (MQ=255)
                                                                                                                                               aaTATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATacc       >  2:4269140/1‑150 (MQ=255)
                                                                                                                                               aaTAGAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATacc       <  2:3085821/150‑1 (MQ=255)
                                                                                                                                                  ataAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATaccacc    >  1:854665/1‑150 (MQ=255)
                                                                                                                                                   taAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCAccg   >  1:1013494/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGTTTATCGGCAATCGACGAGGCAATATAAGCGCCCATGATGGGGATCATAAAGGTGAAGCCAAGGTAACCGATGGATTCAACTACCCAGGTAAACGACGGCGCGCCTTTCGACATATCGGTATACGGCAAACCGAACTGCACCAGCATGTTGGCAAGCGCCACCAGGATACCACCGC  >  CP009273/4084996‑4085293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑