Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 877,776 A→C 5.5% F139V (TTT→GTT)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273877,7760AC 5.5% 649.6 / 2.8 203F139V (TTT→GTT) deoRdeoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
Reads supporting (aligned to +/- strand):  ref base A (106/80);  new base C (3/14);  total (109/94)
Fisher's exact test for biased strand distribution p-value = 2.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACC  >  CP009273/877628‑877924
                                                                                                                                                    |                                                                                                                                                    
tCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGaaa                                                                                                                                                      >  2:1819833/1‑149 (MQ=255)
   aGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAAcgc                                                                                                                                                   <  2:2382794/149‑1 (MQ=255)
   aGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAAcgc                                                                                                                                                   >  2:4146100/1‑149 (MQ=255)
     aTTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAAcgcgc                                                                                                                                                 >  1:651904/1‑149 (MQ=255)
      ttAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAACcgcgcg                                                                                                                                                >  1:1610287/1‑149 (MQ=255)
      ttAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAAcgcgcg                                                                                                                                                <  2:2113714/149‑1 (MQ=255)
        aaaaCAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGc                                                                                                                                              >  1:4321030/1‑149 (MQ=255)
          aaCAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCaa                                                                                                                                            >  1:4128429/1‑149 (MQ=255)
           aCAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAAt                                                                                                                                           <  2:3975773/149‑1 (MQ=255)
            cAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATg                                                                                                                                          >  2:1916108/1‑149 (MQ=255)
             aGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAACATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGACATTCACCACCGCAAAGACACGCGCGGCACTgg                                                                                                                                         >  2:2196425/1‑149 (MQ=255)
             aGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATgg                                                                                                                                         <  2:3592756/149‑1 (MQ=255)
             aGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATgg                                                                                                                                         >  1:328363/1‑149 (MQ=255)
              ggTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATggg                                                                                                                                        <  2:3333175/149‑1 (MQ=255)
                tAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGt                                                                                                                                      >  2:864832/1‑149 (MQ=255)
                tAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGt                                                                                                                                      <  2:797662/149‑1 (MQ=255)
                 aGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCACTGGGGtt                                                                                                                                     >  1:2651749/1‑149 (MQ=255)
                 aGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGtt                                                                                                                                     <  1:1268954/149‑1 (MQ=255)
                   cgcCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtc                                                                                                                                   <  1:2386483/149‑1 (MQ=255)
                   cgcCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtc                                                                                                                                   <  1:2386324/149‑1 (MQ=255)
                    gcCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtct                                                                                                                                  >  2:2572952/1‑149 (MQ=255)
                    gcCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtct                                                                                                                                  >  1:340586/1‑149 (MQ=255)
                     ccTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtctc                                                                                                                                 >  2:177254/1‑149 (MQ=255)
                     ccTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTtctc                                                                                                                                 <  1:1840261/149‑1 (MQ=255)
                      cTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTctct                                                                                                                                <  1:3944066/149‑1 (MQ=255)
                      cTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTctct                                                                                                                                <  2:4063349/149‑1 (MQ=255)
                       tttACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATCTCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGGTGCTGGCGTGAAATTCACCACCGCAAAGACACGCGCGGCAAGGGGGTTTCTCtt                                                                                                                               >  2:2692380/1‑149 (MQ=255)
                        ttACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCttt                                                                                                                              >  2:1949880/1‑149 (MQ=255)
                          aCTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGGTTCTCTTTCa                                                                                                                            >  1:3876889/1‑149 (MQ=255)
                            tGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAgc                                                                                                                          >  1:101983/1‑149 (MQ=255)
                             gaaaTGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTTAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAgcg                                                                                                                         >  1:3631712/4‑149 (MQ=255)
                             gACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAgcg                                                                                                                         >  2:1514164/1‑149 (MQ=255)
                              aCATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAgcgc                                                                                                                        >  1:3221413/1‑149 (MQ=255)
                                  gCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGa                                                                                                                    >  2:874367/1‑149 (MQ=255)
                                     cgcccgccGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAg                                                                                                                 <  2:4246371/149‑1 (MQ=255)
                                     cgcccgccGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAg                                                                                                                 <  2:2511235/149‑1 (MQ=255)
                                        ccgccgCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTa                                                                                                              <  2:4257387/149‑1 (MQ=255)
                                          gccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTAtt                                                                                                            <  2:2898238/149‑1 (MQ=255)
                                          gccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTAtt                                                                                                            <  2:717674/149‑1 (MQ=255)
                                          gccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTAtt                                                                                                            >  1:2441642/1‑149 (MQ=255)
                                          gccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTAtt                                                                                                            <  1:3518400/149‑1 (MQ=255)
                                            cgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAACCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTa                                                                                                          >  1:1497001/1‑149 (MQ=255)
                                                   tAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATa                                                                                                   <  1:3908695/149‑1 (MQ=255)
                                                     aaaaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAAc                                                                                                 <  2:3456284/149‑1 (MQ=255)
                                                     aaaaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAAc                                                                                                 >  1:1945045/1‑149 (MQ=255)
                                                       aaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACaa                                                                                               >  2:1516453/1‑149 (MQ=255)
                                                         gCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAc                                                                                             >  2:819486/1‑149 (MQ=255)
                                                         gCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAc                                                                                             <  2:1525463/149‑1 (MQ=255)
                                                           gATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcgg                                                                                           >  2:2884748/1‑149 (MQ=255)
                                                             tatCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcggcg                                                                                         >  1:26353/1‑149 (MQ=255)
                                                              atCCGGGCAAAAATTATTCAGCGTTTGCTGAACATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcggcgg                                                                                        >  1:430191/1‑149 (MQ=255)
                                                                  gggCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTaaa                                                                                    >  2:128136/1‑149 (MQ=255)
                                                                   ggCAAAAATTATTCAGCGTTTGCTGACAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGACAGGTATTTAGCGAATAACAAACGGCGGTacaa                                                                                   >  2:3408701/1‑149 (MQ=255)
                                                                     aaaaaaTTTTTCAGGGTTTGCTTAAAATCTTTGGTTTTGAAATTGGCGTTGCTGGCGTTAATTTCACCACCGAAAAGAAACGCGCGGAATTGGGTTTTCTCTTTCAGCGCAATAAAGTTTTTTAGCTAATAACAAACGGCGGTAAAAgg                                                                                 <  2:3011332/148‑1 (MQ=255)
                                                                      aaaaaTTATTCAGCGTTTGCTGAAAATCGATGGGTTTG‑AAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCACTGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGa                                                                               >  1:1326185/1‑149 (MQ=255)
                                                                       aaatTTTTTCCGCGTTGGCTGAAAATCGCTGGGTTTGAAACGGGCGTTGCTGGCGTGAAATTCACCCCCGAAAAGAACCGCGCGGCATGGGGGTTTCTCTTTCCGCGCCATAATGGTATTTAGCGAATAACACACGGCGGTAAAAGGGa                                                                               <  2:413064/149‑1 (MQ=255)
                                                                         aaTTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGAtt                                                                             >  2:1421956/1‑149 (MQ=255)
                                                                              ttCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCAtt                                                                        <  2:4321030/149‑1 (MQ=255)
                                                                              ttCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCAtt                                                                        >  2:747233/1‑149 (MQ=255)
                                                                              tacAGCGTTTGCTGAAAATCGTTGGGTTTGAAAAGGGCGTTGCTGGCGTTAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGTTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCAtt                                                                        <  2:2752191/147‑1 (MQ=255)
                                                                                   cGTTTGCTTAAAATCTATGGTTTTGAAATTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGTTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCaa                                                                   <  1:1386146/149‑1 (MQ=255)
                                                                                    gTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAAt                                                                  >  2:3833508/1‑149 (MQ=255)
                                                                                        gCTGAAAATCGATGGGTTTGAAATTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCt                                                              <  2:3601456/149‑1 (MQ=255)
                                                                                         cTGAAACTCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAACGAACCGCGCGGCAAGGGGGTTTCTCTTTCAGCGCCAGAACGGTATTTCGCGAATAACACACGGCGGTAAAAGGGATTTCATTATCAATCGCtt                                                             <  2:918857/149‑1 (MQ=255)
                                                                                         cTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGGGGCATTGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCtt                                                             <  2:3036711/149‑1 (MQ=255)
                                                                                         cTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCtt                                                             <  2:3955023/149‑1 (MQ=255)
                                                                                         cTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCtt                                                             >  2:1332510/1‑149 (MQ=255)
                                                                                           gAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCa                                                           >  1:2564838/1‑149 (MQ=255)
                                                                                            aaaaTCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGACAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaa                                                          >  2:2871821/1‑149 (MQ=255)
                                                                                             aaaTCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAACCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaat                                                         <  2:3221321/149‑1 (MQ=255)
                                                                                               aTCGATGGGTTTGAAATTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGTTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaataa                                                       <  2:3468613/149‑1 (MQ=255)
                                                                                                tCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaataat                                                      >  2:1500152/1‑149 (MQ=255)
                                                                                                tCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaataat                                                      >  1:3125998/1‑149 (MQ=255)
                                                                                                 cgttGGTTTTGAAATTGGGTTTGGTGGGTTGAAATTCACCACCGCAAAGAACCGCGCGGCATGGGGTTTTCTATTTCCGCGCCATAATGGTTTTTTGCTAATAAAAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATc                                                     <  1:4078700/146‑1 (MQ=255)
                                                                                                    tGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCAc                                                  >  1:2552092/1‑149 (MQ=255)
                                                                                                           gAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtgg                                           >  1:201405/1‑149 (MQ=255)
                                                                                                            aaatTGGCGTTGCTGGCGTGAAATTCACCACCGAAAAGAAACGCGGGGCATTGGGTTTACTTTTTCCGCGCAATAATGGTTTTTAGCTAATAAAAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggt                                          <  1:1274712/149‑1 (MQ=255)
                                                                                                            aaaaTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCACTGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggt                                          >  2:2648555/1‑149 (MQ=255)
                                                                                                            aaaaTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGACAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggt                                          >  2:2415881/1‑149 (MQ=255)
                                                                                                            aaaaTGGCGTTGCTGGCGTGAAATACACCACCGAACATAAACGCGCGGCATTGGGGTTTCTCTTTCAGCGCAAGAAAGTTATTTAGCTATTAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggt                                          <  2:1963620/149‑1 (MQ=255)
                                                                                                             aaaTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggtg                                         >  2:1468153/1‑149 (MQ=255)
                                                                                                              attGGCTTTGCTGGCGTTAAATTCACCCCCGCAAAGAAACGCGCGGCATGGGGGTTTCTCTTTCAGCGCCTTAAAGTTATTTAGCTAATAAAAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggtgg                                        <  1:959034/147‑1 (MQ=255)
                                                                                                               agggCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCTGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggtggt                                       <  2:640779/147‑1 (MQ=255)
                                                                                                               aTGGGGTTGGGGGCTTTAAATTAACCACCGAAAAGAACCGCGCGGAATTGGGGTTTCTATTTCCGCGCCAGATTGTTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggtggt                                       <  1:540725/149‑1 (MQ=255)
                                                                                                                  gCGTTGGTGGCTTTAATTACACCACCTCAAAGAACCGCGCGTCATGGGGGTTTCTCTTTCAGCGCAAGAATTGTTTTTAGCTAATAAAAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGcc                                    <  1:3770808/149‑1 (MQ=255)
                                                                                                                    gTTGCTGGCGTGAAATTCACCACCGAAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAATGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCcac                                  <  1:1752256/149‑1 (MQ=255)
                                                                                                                      tGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAg                                >  2:1645076/1‑149 (MQ=255)
                                                                                                                      ggggggCGTTAAATTCACCACCCAAAATACCCGGGGGGCTTTGGGTTTTATCTTTCCGCGCAAGAATGGTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAg                                <  1:1212926/145‑1 (MQ=255)
                                                                                                                         tGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCa                             >  2:1043909/1‑149 (MQ=255)
                                                                                                                             gTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCaaaaa                         <  2:3239570/149‑1 (MQ=255)
                                                                                                                             gTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCaaaaa                         <  2:1390800/149‑1 (MQ=255)
                                                                                                                                  aTTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAATGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAgg                    >  2:2055811/1‑149 (MQ=255)
                                                                                                                                  aTTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAgg                    >  2:1369599/1‑149 (MQ=255)
                                                                                                                                     caccacCGCAAAGAACCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTTTTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTc                 <  2:328363/149‑1 (MQ=255)
                                                                                                                                     caccacCGAAAAGACCCGCGCGGCCCTGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTc                 <  1:623826/149‑1 (MQ=255)
                                                                                                                                      accaccGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCt                >  2:2369760/1‑149 (MQ=255)
                                                                                                                                       ccaccGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTg               <  2:3769301/149‑1 (MQ=255)
                                                                                                                                       ccaccGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTg               >  1:3366357/1‑149 (MQ=255)
                                                                                                                                                 gAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCt     >  1:1799574/1‑149 (MQ=255)
                                                                                                                                                   aaCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTAc   >  2:1618002/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCAAGATTAAAACAGGTAGCGCCTTTACTGACATGCACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACC  >  CP009273/877628‑877924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑