BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,811,504	T→C	5.4%	intergenic (‑10/+53)	argZ ← / ← argY	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,811,504	0	T	C	5.4%	333.4 / 3.2	113	intergenic (‑10/+53)	argZ/argY	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (52/50); new base C (3/3); total (59/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTA  >  CP009273/2811356‑2811651

 gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                      <  2:2322814/149‑1 (MQ=40)

 gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                      >  2:3653051/1‑149 (MQ=40)

  tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                      >  1:600165/1‑148 (MQ=40)

  tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                      <  2:600165/148‑1 (MQ=40)

          gttacctCCGCTCCCTCTTCGAGCCCGTCATTGCTGAAGATGTCGGAGCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTTGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                                                                                                                             <  2:2324635/143‑1 (MQ=14)

          gTTAATTCCGCTACCTCTTCTATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                                                                                                                             <  1:2257699/149‑1 (MQ=255)

          gTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                                                                                                                             <  1:2257639/149‑1 (MQ=255)

            tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTActgc                                                                                                                                           >  1:2409186/1‑149 (MQ=255)

             aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgca                                                                                                                                          <  1:3125444/149‑2 (MQ=255)

             aaTACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                          <  2:1957111/149‑1 (MQ=9)

                tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                     <  1:2967428/149‑1 (MQ=255)

                 ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑ta                                                                                                                                    <  1:3011598/149‑1 (MQ=255)

                 ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑ta                                                                                                                                    <  2:3402615/149‑1 (MQ=255)

                 ccGCTACCTCTTCGATACCTTCAGTGCTGCAGATTTCGGAGAATCTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTACGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑ta                                                                                                                                    <  2:936820/149‑1 (MQ=255)

                 ccGCTACCTCATCGTTACAGACACGGCTGCAGAGTTCTTCTACTATGGCGCATCCGGGAGGATTCGCACCTCGTAGCGCTCCGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑ta                                                                                                                                    <  2:936747/149‑1 (MQ=9)

                     tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACAGTTAATTTACTGCTGA‑‑TAtttt                                                                                                                                >  1:206845/1‑147 (MQ=255)

                     tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                <  1:4128476/149‑1 (MQ=255)

                     tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                <  1:4128464/149‑1 (MQ=255)

                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                               <  1:4324032/149‑1 (MQ=255)

                      aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                               <  1:1516236/149‑1 (MQ=255)

                        ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAc                                                                                                                             <  2:2824351/149‑1 (MQ=255)

                        ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAc                                                                                                                             <  2:2824411/149‑1 (MQ=255)

                         tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGCTGCATCGGGAAATGTCTATTACTGCTGA‑‑TATTGATAcc                                                                                                                            >  2:3104990/1‑149 (MQ=255)

                         tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAGTTTACTGCAGA‑‑TATTtatagc                                                                                                                            >  2:2617733/1‑147 (MQ=255)

                          ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTACTTTActgc                                                                                                                                           >  2:1335070/1‑135 (MQ=255)

                          ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgc                                                                                                                                           <  1:1335070/135‑1 (MQ=255)

                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                       <  2:994355/149‑1 (MQ=255)

                              gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                       <  1:1072814/149‑1 (MQ=255)

                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctact                                                                                                                      >  2:211051/1‑149 (MQ=255)

                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAACGctcct                                                                                                                      >  1:3945778/1‑149 (MQ=255)

                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTAACTGCTGA‑‑TATAGATACAGctaca                                                                                                                      >  1:1437402/1‑148 (MQ=14)

                                 aCCTTCATTGCTGAAGATTTCGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAATTTACTGCAGA‑‑TATTTATACAGcgacaat                                                                                                                    >  2:22780/1‑146 (MQ=14)

                                  ccTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaaa                                                                                                                   <  2:3260395/149‑1 (MQ=255)

                                  ccTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGctacta                                                                                                                     >  1:825654/1‑149 (MQ=11)

                                       aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGCTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAACGCTACTAAAGCCat                                                                                                              >  2:3341719/1‑149 (MQ=255)

                                         tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCgtat                                                                                                            >  1:3008917/1‑149 (MQ=255)

                                          gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATc                                                                                                           <  2:2519541/149‑1 (MQ=255)

                                            tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCaa                                                                                                         >  1:1578368/1‑149 (MQ=255)

                                               aggttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                >  2:2647949/7‑123 (MQ=21)

                                               aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCAAATCAAgaa                                                                                                      >  1:1961233/1‑147 (MQ=255)

                                                  tttCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                   <  2:3160645/149‑1 (MQ=255)

                                                  gttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGTTTCGTAGACGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                   <  2:3160687/148‑1 (MQ=255)

                                                     cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                <  2:2560246/149‑1 (MQ=40)

                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                               <  1:2096604/149‑1 (MQ=40)

                                                      ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                               <  1:2422958/149‑1 (MQ=40)

                                                       gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATGTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTACGAGATgg                                                                                              >  1:771093/1‑149 (MQ=255)

                                                         gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATAACGATACTAAAGCCATATCAAGTAAGAGATGGtt                                                                                            >  1:4103032/1‑148 (MQ=255)

                                                         gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAACGCTACTAAATCAATAACAAGTAAGAGATggag                                                                                            >  1:1927889/1‑147 (MQ=255)

                                                          aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGc                                                                                           <  1:481224/149‑1 (MQ=40)

                                                          aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGc                                                                                           <  2:2734355/149‑1 (MQ=40)

                                                           atatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                          >  2:79821/1‑149 (MQ=40)

                                                            tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCAt                                                                                         >  1:3910565/1‑149 (MQ=40)

                                                                 tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCggg                                                                                    >  1:1791844/1‑149 (MQ=255)

                                                                  gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑tat                                                                                                                                   >  2:1363120/1‑101 (MQ=255)

                                                                         ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCg                                                                            >  2:2132788/1‑149 (MQ=255)

                                                                         ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCg                                                                            >  2:2132718/1‑149 (MQ=255)

                                                                         ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCAAATCAAGTAAGAGATGGTGCATCCGGGAGGATTCg                                                                            >  2:2132787/1‑149 (MQ=255)

                                                                            ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAc                                                                         <  2:1232747/149‑1 (MQ=255)

                                                                             gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAGGCATCGGGAAATTTAGTTTACTGCTGA‑‑TATTGTTACCGCTACTATAGCCATATCAATTAAGAGATGGTGAATCAGGGAGGATTAGacac                                                                        >  1:543930/1‑146 (MQ=11)

                                                                               ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                      <  1:2424194/71‑1 (MQ=32)

                                                                               ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                      >  2:2424194/1‑71 (MQ=32)

                                                                                 cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAcc                                                                                                                            >  2:3697095/1‑93 (MQ=255)

                                                                                 cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAcc                                                                                                                            <  1:3697095/93‑1 (MQ=255)

                                                                                  gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAGACAGCTGAGATACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATCCCGCTACTAAAGCCAGATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGGACCGCCGa                                                                   >  1:1626309/1‑149 (MQ=14)

                                                                                   aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAGCTCCGAc                                                                  >  2:1461797/1‑149 (MQ=255)

                                                                                      cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGc                                                               >  1:3623471/1‑149 (MQ=255)

                                                                                       tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGcta                                                                                                                        <  2:3591394/91‑1 (MQ=255)

                                                                                       tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGcta                                                                                                                        >  1:3591394/1‑91 (MQ=255)

                                                                                         cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                            <  1:1024186/149‑1 (MQ=255)

                                                                                         cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAGCGCCGAGCggagc                                                            >  2:879880/1‑145 (MQ=255)

                                                                                           aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGt                                                          <  2:3380598/149‑1 (MQ=255)

                                                                                           aCCGCGCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGATACGGATGCATCGGGATAATTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGt                                                          <  2:3380123/149‑1 (MQ=255)

                                                                                            ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                         <  2:1390439/149‑1 (MQ=255)

                                                                                            ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                         <  2:1390444/149‑1 (MQ=255)

                                                                                             cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                        >  2:1488476/1‑149 (MQ=255)

                                                                                             cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                        >  2:1488615/1‑149 (MQ=255)

                                                                                              gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTCAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                       >  1:797883/1‑149 (MQ=255)

                                                                                               cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                      >  1:1534714/1‑149 (MQ=255)

                                                                                                tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                     <  2:2497404/149‑1 (MQ=255)

                                                                                                 cGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                    >  2:2281207/1‑149 (MQ=255)

                                                                                                 cGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                    >  1:216676/1‑149 (MQ=255)

                                                                                                       gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                              <  1:933261/149‑1 (MQ=255)

                                                                                                        tAGCCGAGTACTCTATCCCGCTGCGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                             <  1:177864/149‑1 (MQ=255)

                                                                                                        atctcgtgTACTCTATCCAGCTGAGCTACGGATGCATCGGGATATTTTTTTTACTGCTGA‑‑TTTTTATACAGCTACTAAAGACATATAAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                             <  2:1054237/142‑1 (MQ=21)

                                                                                                          gCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                           >  2:412211/1‑149 (MQ=255)

                                                                                                             gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAcagat                                        >  1:1054237/1‑145 (MQ=255)

                                                                                                             gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                        >  1:2362833/1‑149 (MQ=255)

                                                                                                             gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                        >  1:3409510/1‑149 (MQ=255)

                                                                                                              aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                       >  1:2884237/1‑149 (MQ=255)

                                                                                                                  ctctATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                   >  2:567671/1‑149 (MQ=255)

                                                                                                                           gCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                          >  1:4255259/1‑149 (MQ=255)

                                                                                                                              gAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                       <  2:1578368/149‑1 (MQ=255)

                                                                                                                                gCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                     >  2:794320/1‑149 (MQ=255)

                                                                                                                                gCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCCCACGGATCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                     >  2:794319/1‑149 (MQ=255)

                                                                                                                                     ggATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                <  1:383363/149‑1 (MQ=255)

                                                                                                                                        cgCATCGGGAAATTTATTTTTCTGCTGA‑‑TATTGTAACCGCTAATACAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGa             <  2:2234984/148‑1 (MQ=21)

                                                                                                                                           aTCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAac          >  1:705719/1‑149 (MQ=255)

                                                                                                                                               ggAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac      >  1:3526414/1‑149 (MQ=255)

                                                                                                                                               ggAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac      >  1:1346445/1‑149 (MQ=255)

                                                                                                                                                   atttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttta  >  2:4036810/1‑149 (MQ=255)

AGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTA  >  CP009273/2811356‑2811651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`