Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,291 T→G 13.1% C315G (TGC→GGC)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,2910TG13.1% 526.7 / 7.3 213C315G (TGC→GGC) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (76/94);  new base G (30/5);  total (114/99)
Fisher's exact test for biased strand distribution p-value = 9.96e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

ATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTA  >  CP009273/2835144‑2835439
                                                                                                                                                   |                                                                                                                                                    
aTCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTCTTTAAGCAATTAAGCCACGCGCTg                                                                                                                                                     >  1:183141/1‑149 (MQ=255)
  cGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTATTTAAGCTATTAAGCCACGCgcggcg                                                                                                                                                   >  1:2247518/1‑149 (MQ=255)
     tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACACAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCt                                                                                                                                                <  2:4204341/149‑1 (MQ=255)
     tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCt                                                                                                                                                <  1:3441490/149‑1 (MQ=255)
     tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCt                                                                                                                                                <  1:658326/149‑1 (MQ=255)
     tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTCTTTAAGCTATTACGCCACGCGCGGCGCCt                                                                                                                                                >  1:1600285/1‑149 (MQ=255)
     tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTCAGCGATTTAGCCACGCCCGGCGccc                                                                                                                                                >  1:3986688/1‑148 (MQ=255)
              gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgaga                                                                                                                                       >  2:1714512/1‑149 (MQ=255)
                ggggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATg                                                                                                                                     <  2:2463582/149‑1 (MQ=255)
                 gggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGCCGATTTTGTCTCTTTCAGCGATTACGCCTCGCGCTGCGCCTCGGCGGAGATGa                                                                                                                                    >  2:1625409/1‑149 (MQ=255)
                  ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAGATCCTGAAAAACACCGTCGATTTTTTCTCTTTAAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                   >  1:3221779/1‑149 (MQ=255)
                  ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTCTTTGAGCTATTACGCCCCGCGCGGCGCCCCCGCGGGGATgta                                                                                                                                   >  1:4142837/1‑147 (MQ=255)
                     aCGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTAAGCCGCGCGCGGCGCCACGGCGGAGATGAAAGc                                                                                                                                >  1:1036543/1‑149 (MQ=255)
                      cGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGcc                                                                                                                               <  2:3470565/149‑1 (MQ=255)
                      cGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTAAGCCTAGCGCAGCGCCGCGGCGGAGATGTACGcc                                                                                                                               >  1:3908159/1‑149 (MQ=255)
                      cGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTGTTTCAACTATTACACCCCGCCCAGCGCCCCGGCGGAGCTGaaaccc                                                                                                                               >  1:3673863/1‑145 (MQ=255)
                        tATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTATTTCAGCAATTACGCCGCGCGCTGCGCCACGGCGGAGATGAACGCcaa                                                                                                                             >  1:1376375/1‑149 (MQ=255)
                          tCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTATGTTTAAGCTATTAAGCCTCGCGCTGCGCCTCGGCGGAGATGAACCCcaaca                                                                                                                           >  1:3736336/1‑149 (MQ=255)
                            cGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTTCGCCGCGCGCTGCGCCGCGGCGGGGATGAACCCCcacaac                                                                                                                         >  1:1761872/1‑149 (MQ=255)
                              gCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcag                                                                                                                       <  1:3582825/149‑1 (MQ=255)
                                  aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTATCTTTCAGCTATTACGCCTCGCGCTGCGCCACGGCGGGGATGAACGCCAACAACAGCAGt                                                                                                                   >  1:3218393/1‑149 (MQ=255)
                                  aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTAAGCCACGCGCTGCGCCTCGGCGGAGCTGAAACCCAACAACAGCAGt                                                                                                                   >  2:2586932/1‑149 (MQ=255)
                                         ccGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTAAGCTATTAAGCCTCGCGCGGCGCCGCGGCGGAGATTAACGCCAACAACACCCCTGCGGCGa                                                                                                            >  1:4304320/1‑149 (MQ=255)
                                          cgcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGaa                                                                                                           <  1:3729099/149‑1 (MQ=255)
                                           gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTCAGCTATTACGCCACGCGCTGCGCCGCGGCGGCGTTGAACGCCAACAAAAGCAGTGCGGCGAAc                                                                                                          >  1:1416799/1‑149 (MQ=255)
                                           gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTATATTTAAGAAATTAAACCGCGCGCTGCGCCACGGCGGAGATTAAAGCCAACAAAACCAGTTCGGCGAAc                                                                                                          >  1:3596905/1‑149 (MQ=255)
                                             gTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgt                                                                                                        <  2:3949518/149‑1 (MQ=255)
                                             gTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTCTTTCAGCTATTACGCCGCGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgg                                                                                                        >  2:1212199/1‑148 (MQ=255)
                                             gTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTATCGTTAAGCGATTAAGCCCCGCGCGGCGCCACGGCGGAGATGAAAGCCCACAACACCACTGCGGCGCAccg                                                                                                        >  1:3396444/1‑147 (MQ=255)
                                              tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtg                                                                                                       <  2:354405/149‑1 (MQ=255)
                                              tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGAACGCCAACAAAAGCAGTGCGGCGAACgtg                                                                                                       >  2:2368155/1‑149 (MQ=255)
                                              tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACACCAGTGCGGCGCACgtg                                                                                                       >  1:3770602/1‑149 (MQ=255)
                                               aTTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTATTTCAGCTATTAAGCCACGCGCTGCGCCTCGGCGGAGATGAACGCCCACAACACCAGTGCGGCGAACgtgg                                                                                                      >  1:1020021/1‑149 (MQ=255)
                                                ttCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtggt                                                                                                     >  2:1726750/1‑149 (MQ=255)
                                                 tCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTATCTTTAAGCTATTACGCCACGCGCGGCGCCCCCGCGGAGATTAAAGCCAAAAACAGCACTGCGGCGCACggggtg                                                                                                    >  1:611061/1‑149 (MQ=255)
                                                  ccGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCGATTTCGCCTCGCGCGGCGCCACGGCGGAGATGAACGCCCACCACAGCAGTGCGGCGACAGTGGTGa                                                                                                   >  2:2228999/1‑149 (MQ=255)
                                                   cgcgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTATCTTTCAGCGATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAAAGCCAACAACAGCAGTGCGGCGAACGTGGTGaa                                                                                                  >  2:1982550/1‑149 (MQ=255)
                                                    gcgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCAGCGATTACGCCACCCGCTGCGCCACGGCCGCGATGAAAGCCAACAACACCACTGCGGCGAACGGGGTGaaa                                                                                                 >  1:1729522/1‑149 (MQ=255)
                                                      gAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATc                                                                                               <  2:2303484/149‑1 (MQ=255)
                                                        aaaaGGGGTAACCATCAACAACGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGc                                                                                             <  1:874511/149‑1 (MQ=255)
                                                          aaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTg                                                                                           <  2:3680409/149‑1 (MQ=255)
                                                          aaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTATATTTAAGCTATTAAGCCACCCGCGGCGCCACGGCGGAGATGAACGCCAAAAACAGCAGTGCGGCGCACGGGGTGaaaaagcgg                                                                                           >  1:958162/1‑143 (MQ=255)
                                                             gggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGt                                                                                        <  1:90411/149‑1 (MQ=255)
                                                              ggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTa                                                                                       <  1:1232365/149‑1 (MQ=255)
                                                              ggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTGAGCGATTAAGCCGCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGGGCGGCGAACGTGGTGAAATCGCTGCGTa                                                                                       >  2:1483857/1‑149 (MQ=255)
                                                               gTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTaa                                                                                      <  1:2666772/149‑1 (MQ=255)
                                                                tAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAAt                                                                                     <  1:1778798/149‑1 (MQ=255)
                                                                tAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTAAGCTATTACGCCTCGCGCTGCGCCACGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGCACGGGGTGAAAACGCTGCGTaaa                                                                                     >  2:822688/1‑148 (MQ=255)
                                                                 aaCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATc                                                                                    <  2:3751024/149‑1 (MQ=255)
                                                                  aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATcc                                                                                   <  2:589674/149‑1 (MQ=255)
                                                                     aTCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTa                                                                                <  2:2644739/149‑1 (MQ=255)
                                                                     aTCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCGCTTTCATCGATTACGCCTCGCGCGGCGCCGCGGCGGAGTTGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGTAATCGCTGCGTAATACGTa                                                                                >  1:2148172/1‑149 (MQ=255)
                                                                       caacaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCAGCTATTAAGCCGCGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGTAATAGCTGCGTAATCCGTATc                                                                              >  2:3364750/1‑149 (MQ=255)
                                                                        aacaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTAAGCTATTACGCCGCGCGCGGCGCCGCCGCGGCGATGAACGCCAACAACAGCAGTGCGGCGAACGTTGTGAAAAAGCTGCGTAATACGTATca                                                                             >  1:702431/1‑148 (MQ=255)
                                                                            aaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACac                                                                         >  2:3401464/1‑148 (MQ=255)
                                                                            aaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTAAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGTACGCCAACAACAGCAGTGCGGCGAACGTGGTGTAATAGCTGCGTAATCCGTATATACAg                                                                         >  2:1617348/1‑149 (MQ=255)
                                                                              gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGt                                                                       <  2:3011437/149‑1 (MQ=255)
                                                                              gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCATTCAGCTATTAACCCACCCGCGGCGCCACCGCGGAGATGTACCCCAACAACAGCAGTGCGGCGAACGTGGGGGAATCGCGGCGTAAACCGTATAGACAggg                                                                       >  1:2100944/1‑148 (MQ=255)
                                                                                aCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTCATCTATTAAGCCGCGCGCGGCGCCGCGGCGGAGTTGAAACCCAACAACAGCAGTGCGGCGTAAGTGGTGAAATAGCTGCGGAATCCGTATCTAAAGGTGa                                                                     >  1:3234493/1‑149 (MQ=255)
                                                                                aCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                     <  2:4322035/149‑1 (MQ=255)
                                                                                  cGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGc                                                                   <  1:350630/149‑1 (MQ=255)
                                                                                    ggCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGa                                                                 <  2:2897888/149‑1 (MQ=255)
                                                                                    ggCGATGATGAAATCCTGAAAAACACCGTCGATTGTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGa                                                                 <  1:374916/149‑1 (MQ=255)
                                                                                     gCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGAc                                                                <  1:2588033/149‑1 (MQ=255)
                                                                                        atgatgAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActgg                                                             <  2:4142837/149‑1 (MQ=255)
                                                                                         tgatgaAATCCTGAAAAACACCGTCGATTTTTTCTATTTGAGCGATTACGCCTCGCGCGGCGCCTCGGCGGAGATGAAAGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATAGCTGCGTAATCCGTATCTACAGGTGAGCGAcgggg                                                            >  1:3587148/1‑149 (MQ=255)
                                                                                           atgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCTGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggc                                                          <  1:283055/149‑1 (MQ=255)
                                                                                           atgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggc                                                          <  2:440677/149‑1 (MQ=255)
                                                                                                    tGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAAtt                                                 <  1:2871562/149‑1 (MQ=255)
                                                                                                         aaCACCGTCGATTTTGTCTCTTTCCGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTTAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATcc                                            <  1:2210313/149‑1 (MQ=255)
                                                                                                         aaCACCGTCGATTTTGTATCTTTAAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCCAAAACAGCAGTTCGGCGAAAGTGGTGAAATAGCTGCGTAAAACGTATATACAGGTTAGCGACTGGGGCGGGGGAAATGAtac                                            >  2:110939/1‑147 (MQ=255)
                                                                                                           cacCGTCGATTTTGTCTCTTTCAGCTATTACGCCGCGCGCGGCGCCGCCGCGGAGATGAACCCCAACAACAGCAGTGCGGCGCACGTGGTGAAATCGCTGCCTAATCCCTATAAACAGGTGAGCGACGGGGGCGGGGGCAATGATCCAc                                          >  2:2307406/1‑149 (MQ=255)
                                                                                                            acCGTCGAGTTTGTCTCTTTCAGCAATTACGCCTCGCGCAGCGCCTCGGCGGAGATGAACCCCAACAAAAGCAGTGCGGCGAACGTGGTGAAATAGCAGCGTAAACCGTAACTACACGTGAGCCACTTGGGCTGGGGAATTGATacaca                                         >  2:657425/1‑148 (MQ=255)
                                                                                                             ccGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTc                                        <  1:851927/149‑1 (MQ=255)
                                                                                                               gTCGAGTTTGTCTCTTTCAGCTATTACGCCAAGCGCGGCGCCACGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACCTGGTGAAAACGCAGCGTAATACGTATCTACAGGTGAGCGCCAGGGGCTGGGGAATTTATACACTCgg                                      >  1:3100237/1‑149 (MQ=255)
                                                                                                                tCGATTTTGTCTCTTTCAGCTATTACGCCACGCGCTGCGCCTCGGCGGAGATGAACGCCAAAAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGt                                     >  1:3030998/1‑149 (MQ=255)
                                                                                                                    ttttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGc                                 >  2:3415269/1‑149 (MQ=255)
                                                                                                                       tGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACTGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTa                              <  1:2582536/149‑1 (MQ=255)
                                                                                                                       gttctgTTTCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATTAACGCCAACAACAGCTGTGCGGCTAACGTGGTGAAATCGCTGGGTAATCCGTATCTACTGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTa                              <  1:2581466/147‑1 (MQ=255)
                                                                                                                        gTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTAt                             <  2:2148172/149‑1 (MQ=255)
                                                                                                                         tctcTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                            >  2:1361707/1‑149 (MQ=255)
                                                                                                                         tatctTTCAGCTATTCCGCCTCGCGCGGCGCCTCGGCGTAGTTGAACGCCAACAACAGCTGTGCGGCGAACGTGTTTAAATCGCTGCTTAATCCTTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                            <  1:1110019/147‑1 (MQ=255)
                                                                                                                           tctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACCGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCAc                          <  2:2539494/149‑1 (MQ=255)
                                                                                                                           tctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCAc                          <  2:1693258/149‑1 (MQ=255)
                                                                                                                              ttCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCAt                       >  1:1890820/1‑149 (MQ=255)
                                                                                                                                cAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGa                     >  2:1059845/1‑149 (MQ=255)
                                                                                                                                  gCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                   <  1:2432935/149‑1 (MQ=255)
                                                                                                                                  gCTATTACGCCTCGCGCGGCGCCTCGGCGGAGATTAACGCCAACAACAGCAGTGCGGCTAACGTGTTTAAATCGCTGCGTAATCCGTATATACTGGTGCGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                   <  1:3973810/149‑1 (MQ=255)
                                                                                                                                    tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAatat                 >  2:271355/1‑149 (MQ=255)
                                                                                                                                    tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAatat                 >  1:4325718/1‑149 (MQ=255)
                                                                                                                                      ttACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga               <  1:2752411/149‑1 (MQ=255)
                                                                                                                                       tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgat              <  2:560936/149‑1 (MQ=255)
                                                                                                                                       tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgat              >  2:3371330/1‑149 (MQ=255)
                                                                                                                                        aCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg             >  2:3511678/1‑149 (MQ=255)
                                                                                                                                        aCGCCTCGCGCGGCGCCTCGGCGGATATTAACGCAAACAACAGCAGTGCGGCTAACGTGTTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg             <  1:2182610/149‑1 (MQ=255)
                                                                                                                                          gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa           <  2:811122/149‑1 (MQ=255)
                                                                                                                                           ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTAc          <  2:43261/149‑1 (MQ=255)
                                                                                                                                           ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTAc          <  2:69027/149‑1 (MQ=255)
                                                                                                                                               gcgcTGCGCCTCGGCGGAGATGAACGCAAAAAACAGCAGTGCGTCGTACGTTGTGAAATCGATGCGTAATCCGTATCTACAGGTGAGCGACTGGGTCTGGGTAATTGATCCACTCGGTCTGCTTATCACCATGAATATGATGTACGATc      <  2:2452293/149‑1 (MQ=255)
                                                                                                                                                  cTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt   >  2:1917403/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTA  >  CP009273/2835144‑2835439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑