Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 10.7% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG10.7% 502.4 / 9.5 210Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (69/112);  new base G (26/3);  total (95/115)
Fisher's exact test for biased strand distribution p-value = 1.24e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTC  >  CP009273/3721339‑3721633
                                                                                                                                                   |                                                                                                                                                    
aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTaa                                                                                                                                                    >  1:890033/1‑149 (MQ=255)
 aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑CCCCCCCCGTACGGTCAt                                                                                                                                                   >  2:4039889/1‑149 (MQ=255)
  gCGGAATAACGCTCGGAATGTGGTTTTTGCGACTGTTCTAACGTTAGTTGCGGAAAAAAGTCATTGAGCTATTTCTCTGTTTTCGCGGCGTTCTGCCCCAGCGTTGCTGGGCCCTTTGCTGGCCCAACAT‑CCCCCCCCGTACGGTAATc                                                                                                                                                  <  2:3869735/149‑1 (MQ=255)
    ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGa                                                                                                                                                <  1:2838086/149‑1 (MQ=255)
    ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGa                                                                                                                                                <  1:2838047/149‑1 (MQ=255)
    ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGa                                                                                                                                                <  1:3045655/149‑1 (MQ=255)
    ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGACGGTCATCgc                                                                                                                                                >  1:2834065/1‑148 (MQ=255)
          aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTCCGGGCATCGAgctgct                                                                                                                                          >  1:1413274/1‑149 (MQ=255)
          aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                          <  1:3273319/149‑1 (MQ=255)
          aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTACGGTCTTCGAgctgct                                                                                                                                          >  1:1413257/1‑149 (MQ=255)
            gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                                        <  2:1383612/149‑1 (MQ=255)
              tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCGCCGGCCGGGCCTCGAgctgcggggg                                                                                                                                      >  1:489981/1‑144 (MQ=255)
                ggcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCTATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                    <  1:2213760/148‑1 (MQ=255)
                cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                    <  2:2023055/149‑1 (MQ=255)
                     cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                               <  2:4134215/149‑1 (MQ=255)
                       ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGTAATCGCGCTGCGGACCGCTGagagc                                                                                                                             >  1:95981/1‑145 (MQ=255)
                       ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CGCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGGTATc                                                                                                                             >  2:1604200/1‑149 (MQ=255)
                        gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                            <  1:3573708/149‑1 (MQ=255)
                        gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAAGCGAGCTGCTGACCGCTGGTCTcc                                                                                                                            >  2:4247491/1‑149 (MQ=255)
                          aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGTGCTCGAGGTGCGGGCCGCTGGTAGCCGc                                                                                                                          >  1:979809/1‑149 (MQ=255)
                          aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGTCATCGTGGTGCTGACCGCGGCTATCCGc                                                                                                                          >  2:3269508/1‑149 (MQ=255)
                           gATGCGACTGTTCTAACTGTATTTGAGGCAACAATTCAATGATCGATTTCTCTGGCTTCGCCTCGATCTTCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCTAGCTGCTGAACGCTGATATCCGcc                                                                                                                         <  2:550975/149‑1 (MQ=255)
                           gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                                                         <  2:2407161/149‑1 (MQ=255)
                           gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                                                         <  1:1735272/149‑1 (MQ=255)
                           gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                                                         <  1:3331879/149‑1 (MQ=255)
                            aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGGAGCGGGGTGCTGCCCGCGGATAGCCGCCa                                                                                                                        >  1:650558/1‑149 (MQ=255)
                                 aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                   <  1:4044829/149‑1 (MQ=255)
                                 aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                   <  2:2842663/149‑1 (MQ=255)
                                 aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGGCGGTGGTCGAGGTGCGGGCCGGGGAGGTccgcccgcggc                                                                                                                   >  1:2798018/1‑146 (MQ=255)
                                  cTGGTCTAACGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                  <  2:2626373/149‑1 (MQ=255)
                                                gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCGGCTGGTATGCGGCAGCATCGGGCGGCAGTTCTCa                                                                                                    >  1:223859/1‑149 (MQ=255)
                                                gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                                                    <  1:1297708/149‑1 (MQ=255)
                                                gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                                                    <  1:1297641/149‑1 (MQ=255)
                                                      gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                                              <  1:1379450/149‑1 (MQ=255)
                                                      gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGGTGAGATCCGCCCGCATCGGACGCCAGTACTCCCTAcgc                                                                                              >  1:2627495/1‑149 (MQ=255)
                                                        aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCCCCCCGTGCGGTGGTCGGGCTGCTGTCCGCTGGTCTCCGCCCGCGTCTGACGCCAGTACTCGCTgcgcgc                                                                                            >  1:3482026/1‑149 (MQ=255)
                                                        aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGGAATCGAGCTGCTGGCCGCTGATATCCGCCCGCCTCTGGCGCCAGTACTCACTAGgcgc                                                                                            >  2:1569314/1‑149 (MQ=255)
                                                        aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATCTCCGCCCGCAGCGGACGCCAGTACTCACTACgcgc                                                                                            >  2:3546001/1‑149 (MQ=255)
                                                        aacaaGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACgcgc                                                                                            <  2:3229284/149‑1 (MQ=255)
                                                         acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGG‑CCCCGCCCGTGCGGTAAGCGAGCTGCTGACCGGGGATTGCCGCCAGCAGCTGACGCGCGGTCTCTCTACGcgcc                                                                                           >  1:1822825/1‑149 (MQ=255)
                                                             ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGTCCTCGAGCTGCTGACCGCGGATATCCGCGAGCCTCTGTCGCGCGTACTCACTCCGcgccccgc                                                                                       >  2:3850025/1‑149 (MQ=255)
                                                                aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGGCGGTCCTCGGGCTGCGGACCGCGGGTCTCCGCCGGCTGCTGACGCCAGGGCGCGCTACGCgccccgcccc                                                                                    >  2:4203413/1‑149 (MQ=255)
                                                                 aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                   <  1:1980470/149‑1 (MQ=255)
                                                                   gAGCGATTTCTCTGGTTTCGCCGCTATCTGCGCCTGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                                 <  2:1667504/149‑1 (MQ=255)
                                                                   gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCCCCCCGGACGGTAAGCGAGCTGCTGACCGCGGTTAGCCGCCAGCATCTGACGCCCGTGCTCACTACGCGCCCCGCCCCCaa                                                                                 >  1:3777880/1‑149 (MQ=255)
                                                                      cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                                                              <  2:381949/149‑1 (MQ=255)
                                                                       gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTGAGCGCGCTGGGGACCGCTGATCTGCGCCAGCGTCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCACTccg                                                                             >  2:1870708/1‑147 (MQ=255)
                                                                        aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGGCGGTCATCGAGCTGCGGACCGCGGAGATCCGCCAGCAGCTGACGCCAGTGCTCACTACGCGCCCCGcccccccgcaac                                                                            >  1:2598927/1‑142 (MQ=255)
                                                                        aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGGGTCGAGCTGCTGGCCGCGGGTATCCGCCGGCAGCTGAGGCCGGGCCTCACTACGCGCCCCGCCCCCaagccac                                                                            >  2:4266876/1‑144 (MQ=255)
                                                                         tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                            <  2:1808801/149‑1 (MQ=255)
                                                                          ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCTCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                          <  2:4178219/149‑1 (MQ=255)
                                                                           tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                         <  1:3120218/149‑1 (MQ=255)
                                                                           tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                         <  1:2613079/149‑1 (MQ=255)
                                                                           tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                         <  1:2685627/149‑1 (MQ=255)
                                                                             tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                       <  1:1109274/149‑1 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGCCGGTAATCGTGCTGCGGTCCGCGGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCAGCAGCGGATCACTTTGc                                                                >  1:1429124/1‑149 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  1:3135788/149‑1 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  1:3114640/149‑1 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  1:2296473/149‑1 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  2:2571268/149‑1 (MQ=255)
                                                                                        cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGACTCGAGCTGCGGGCCGCTGATCTCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACGCGTTGCgggt                                                            >  2:3429001/1‑147 (MQ=255)
                                                                                        cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                            <  1:1843551/149‑1 (MQ=255)
                                                                                          cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa                                                          <  2:336256/149‑1 (MQ=255)
                                                                                           gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGAATCGGGCTGCTGTCCGCTGTTCGCCGCCAGCTGCGGGCGCCCGTGCGCCCTCCGCGCCCCGCCCCCGAGCAGCGGCACACGGTGCggggtta                                                         >  2:609870/1‑144 (MQ=255)
                                                                                           gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATGTCCGCCCGCATCTGTCGCCCGTACGCACTCCGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTag                                                         >  1:1192506/1‑148 (MQ=255)
                                                                                            aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                        >  1:4330380/1‑149 (MQ=255)
                                                                                            aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTGATCGTGCTGCTGACCGCGGTTTTCCGCCAGCATCTGACGCCAGTACGCACGTCGCGCCCCGCCCCCAATCAACGGAACACGTTGCGGTTTAAt                                                        >  2:117903/1‑149 (MQ=255)
                                                                                             tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCCCCCCGTACGGTACTCGTGCTGCTGTGCGCTGATTGCCGGCCGCATCGGCCGCCAGGGCTCTCTACGCGCCCCGCCCCCCAGGAACGGAACACTGTGCgggtgacta                                                       >  2:2668851/1‑142 (MQ=255)
                                                                                             tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCGGATATCCGCCAGCCTCTGACGCCAGTCCTCACTACGCGCCCCGCCCCCCCGCAACGGCACACGGTGCGGGTGAAtg                                                       >  1:4024510/1‑148 (MQ=255)
                                                                                             tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                       <  2:2219834/149‑1 (MQ=255)
                                                                                             tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                       <  2:2220041/149‑1 (MQ=255)
                                                                                             tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                       <  1:1487798/149‑1 (MQ=255)
                                                                                                   ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGGATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCCAGCCACGTACCACTGTGCGGGTTGATACCGccg                                                 >  1:3509673/1‑147 (MQ=255)
                                                                                                   ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCAGCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGGACCACTTTGCGGTTTACTACCGCAg                                                 >  1:3792966/1‑149 (MQ=255)
                                                                                                     aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTTATACCGCAGGc                                               >  1:3179870/1‑149 (MQ=255)
                                                                                                         ttGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCCCCGTGCGGGCAGCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTAGTCACTCCGCGCCCCGCCCCCACTCGACGGAACACTGTGCGGTTGATTCCGGCAGGccggc                                           >  2:3717055/1‑145 (MQ=255)
                                                                                                             tgcGCCCAGTGCTGGCCCCACAC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                       <  2:530888/149‑1 (MQ=255)
                                                                                                              gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc                                      <  1:2873113/149‑1 (MQ=255)
                                                                                                              gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc                                      <  1:3347720/149‑1 (MQ=255)
                                                                                                                gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTTCGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCCATCCACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGAcct                                    >  2:1004132/1‑147 (MQ=255)
                                                                                                                 cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                   <  1:1167888/149‑1 (MQ=255)
                                                                                                                  ccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACCTcc                                  >  1:2403126/1‑149 (MQ=255)
                                                                                                                   cAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                 <  1:757818/149‑1 (MQ=255)
                                                                                                                      tGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                              <  2:19720/149‑1 (MQ=255)
                                                                                                                       gCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc                             <  1:3015871/149‑1 (MQ=255)
                                                                                                                          ggCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                          >  1:2037429/1‑149 (MQ=255)
                                                                                                                          ggCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                          <  2:1106020/149‑1 (MQ=255)
                                                                                                                             cccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                       <  1:784434/149‑1 (MQ=255)
                                                                                                                              ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                      >  2:2973168/1‑149 (MQ=255)
                                                                                                                                 caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc                   <  2:271809/149‑1 (MQ=255)
                                                                                                                                 caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc                   >  2:2181761/1‑149 (MQ=255)
                                                                                                                                     ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                >  2:2892944/1‑149 (MQ=255)
                                                                                                                                     ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                >  2:1338670/1‑149 (MQ=255)
                                                                                                                                     ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                >  1:408541/1‑149 (MQ=255)
                                                                                                                                           ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGTATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc          <  2:3509673/149‑1 (MQ=255)
                                                                                                                                           ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc          <  1:3725710/149‑1 (MQ=255)
                                                                                                                                              tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg       >  2:733838/1‑149 (MQ=255)
                                                                                                                                                cGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGcc     <  2:462681/149‑1 (MQ=255)
                                                                                                                                                  gTAATCGAGCTGCTGACCGCAGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAAACTTAGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCCATCTGCCCGCGCAAAACCCACGCCtt   >  2:3244243/1‑149 (MQ=255)
                                                                                                                                                   tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc  <  2:1768833/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTC  >  CP009273/3721339‑3721633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑