BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,340	A→C	14.5%	Q12H (CAA→CAC) ‡	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,340	0	A	C	14.5%	448.9 / 14.7	223	Q12H (CAA→CAC) ‡	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (118/65); new base C (9/30); total (127/96)
Fisher's exact test for biased strand distribution p-value = 4.02e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      >  2:4176458/1‑149 (MQ=255)

  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  2:1748858/1‑149 (MQ=255)

  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    <  1:577169/149‑1 (MQ=255)

  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    <  1:2736735/149‑1 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGACCCgcg                                                                                                                                                 >  1:2234742/1‑147 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  2:2392044/149‑1 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  1:246342/149‑1 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  1:2401325/149‑1 (MQ=255)

     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGt                                                                                                                                                 >  1:75623/1‑149 (MQ=255)

         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgcg                                                                                                                                             >  1:1042166/1‑149 (MQ=255)

         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                             >  2:2735366/1‑149 (MQ=255)

           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                                                                                                                           >  2:2804857/1‑149 (MQ=255)

             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCaca                                                                                                                                         >  1:210710/1‑147 (MQ=255)

             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCATTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGa                                                                                                                                         >  2:914313/1‑149 (MQ=255)

              tttATCTCATTTAAATATTTAGATGTGGAGGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        <  2:1481476/149‑1 (MQ=255)

               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCCGaaa                                                                                                                                       >  1:2967692/1‑147 (MQ=255)

               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCCGAca                                                                                                                                       >  1:232707/1‑148 (MQ=255)

                tatataaTTTAAATATTTAGATGAGGATGTGGAAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTc                                                                                                                                      <  2:2318299/143‑1 (MQ=255)

                 aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGACAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                     >  2:3311707/1‑149 (MQ=255)

                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCCCAGACTCaa                                                                                                                                    >  2:1229830/1‑149 (MQ=255)

                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACaa                                                                                                                                    >  1:2410790/1‑149 (MQ=255)

                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGGTGCGCAGACACaa                                                                                                                                    >  1:2174108/1‑149 (MQ=255)

                   ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaa                                                                                                                                   >  2:2722303/1‑149 (MQ=255)

                     cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCCGTGCGCAGACACaaaaa                                                                                                                                 >  1:1845787/1‑148 (MQ=255)

                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  2:707999/1‑149 (MQ=255)

                        ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCCAACACaaaaaaac                                                                                                                              >  1:2717852/1‑145 (MQ=255)

                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                            >  2:2393037/1‑149 (MQ=255)

                           aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGaa                                                                                                                           >  2:2596977/1‑149 (MQ=255)

                           aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACacagaa                                                                                                                           >  1:4275680/1‑144 (MQ=255)

                            atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          >  2:2025291/1‑149 (MQ=255)

                            atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAAAACCGAAc                                                                                                                          >  1:2007509/1‑149 (MQ=255)

                              atTTTGTTGTGGTTGTGGAAAAGGTTTGCTTTTCTCTTTCGGTCTGATAAAAGGCCCGCTTTGCTACCACGGGTTTGTTTTCGAGTTCTGTTTCCACCCTCGAGTCAGAAAATGCGAAACGGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                        <  2:1165740/149‑1 (MQ=255)

                               tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                       >  2:2754842/1‑149 (MQ=255)

                               tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAATGa                                                                                                                       >  2:2301990/1‑149 (MQ=255)

                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                    >  2:467893/1‑149 (MQ=255)

                                    aTGAGGTTGTGGCAAAGGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  <  2:3512431/149‑1 (MQ=255)

                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAACAGCGAACTGATTTAcc                                                                                                                 >  2:1654480/1‑149 (MQ=255)

                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                 >  2:2107322/1‑149 (MQ=255)

                                       tggttgtgGCAAACGTTTGATTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTGTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                               <  2:346561/145‑1 (MQ=255)

                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  2:2626625/149‑1 (MQ=255)

                                         gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCAACCGGTTGCGCAGACTCAAAAAAGACAACTGAttttaaggct                                                                                                             >  1:1935620/1‑142 (MQ=255)

                                          ttgtgGCACTGGTTTTCTTTTCTCTGGCGGTCAGACAAATGGCCCGATTTGCTACCCCGGTATTGTTTTCGCTGTCTGTTTCCACCCTCGAGTCATAAAATGCGCACCCGGGCCCCCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                            <  1:1964029/148‑1 (MQ=255)

                                          aTGTGGCACCCGTTTGCGTCACTCTGACGGCCAGCTAAACTGCCCGCTTTGCTACCACGGGAGTGTTTGCGCTGTCTGTTTCCACCCTCGAGTCAGAAAATCCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                            <  2:2389215/149‑1 (MQ=255)

                                                cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt                                                                                                      <  2:3542788/149‑1 (MQ=255)

                                                  aaCTTTTGCTTTTCTCTTTCGGTCAGCTAAATTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    <  2:2519488/149‑1 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCCGACTCAAAAAAGCGAACAGAATTACAGTCTTGAAGAtag                                                                                                    >  1:2724420/1‑147 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCg                                                                                                    >  2:547023/1‑149 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCCGTTCGCACACACAAAAAAACCAACAGATTTAACGGCTTGAACATCg                                                                                                    >  1:2630566/1‑149 (MQ=255)

                                                         gCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGATGCGCACACACAAAACACCGAACAAATTTAACGACATTAAAATCGTcccccc                                                                                             >  1:1533701/1‑148 (MQ=255)

                                                           ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACACAAAACAGCGAACAGATTTAAAGTCATGAAGATCGTCcgccgca                                                                                           >  1:2901384/1‑148 (MQ=255)

                                                            tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCCGACTCAAAACAGCGAACAGATTTAACGTCATGAAGATAGTCcgccgcaa                                                                                          >  1:681365/1‑147 (MQ=255)

                                                            tgtcactTTCGGTCAGCTAAAATGCCCGCTTTGCTAACACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGACAATGCGCCACCGGTTGAGCAGACTCAAAACAGCGAACTGATGTCCCGTCTGGAAGTGCGTCCGCCGCtt                                                                                          >  2:3788725/6‑149 (MQ=255)

                                                                tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTc                                                                                      >  2:3623306/1‑149 (MQ=255)

                                                                  tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCCCTTCCGCaa                                                                                    >  1:1643512/1‑149 (MQ=255)

                                                                   ttCGTTCAGCTAAATTGCCCGCTTTTCTACCAGGGGATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                   <  2:567491/149‑1 (MQ=255)

                                                                      gttccgatAACATGCCGGATTTGCTACCGGGGGTTTTTTTTCGTTTTATGTTTCCCCCCTCTAGTAACAAATTGCGCAACGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                <  1:141289/142‑1 (MQ=255)

                                                                      ggTCATCTAAACTGCCCGTTTTGCTACCTGGGTTTTGTTTTGGTTTTGTTTTTCCACCCTCGTGTAATAAATTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                <  1:1985267/149‑1 (MQ=255)

                                                                         cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCGGTTGCGCAGACACAAAACATCGAACTGATTTTCCGTCTTGTTTATCGTGCGCCGCGGCCTCAAAcccggg                                                                             >  1:788109/1‑146 (MQ=255)

                                                                          acaaaaaaCGCCCGCTTTCCTACCTGGGTATTTTTTTCGTTTTCTGTTACCCCCCTCGAGTCATAAAATGCCAAACGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  1:4121543/145‑1 (MQ=255)

                                                                          aGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  2:806506/149‑1 (MQ=255)

                                                                           gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTTCCGTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           >  1:624261/1‑149 (MQ=255)

                                                                             tAAAATGTCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAATCCCTGTTTgc                                                                         >  2:286604/1‑149 (MQ=255)

                                                                             tAAAATGTCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         >  2:3038542/1‑149 (MQ=255)

                                                                             tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTgc                                                                         >  2:3847577/1‑149 (MQ=255)

                                                                              aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCATGAAGATCGTCCGCCGCGTCCACAAACCCAGTTTtcc                                                                        >  1:3958725/1‑149 (MQ=255)

                                                                              aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCAGTTTgcc                                                                        >  1:324124/1‑149 (MQ=255)

                                                                                aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCGGACAAAAAAAACCGAACAGATTTTACCTCGTGAAGATAGTCCGCCGCGTCCTCAAACCCTGTGTgacgc                                                                      >  1:116593/1‑149 (MQ=255)

                                                                                  tGCCGGTTTTGCGACCATGTGTTTTTTTTCTTTTTTTTTTACCCCCCTCTAGTCACAAATTGCGCACCCGTTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                    <  1:972813/149‑1 (MQ=255)

                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAAAAGATTTAACGTCTTGTAGATAGTACGCCGCGTCCACAAACCCTGTTTGGCGCCt                                                                    >  1:4253284/1‑149 (MQ=255)

                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAACAGCGAACAGGGTTACACTCTTGAATTTAGTCCGCCGCATCCTCAAAACCGGTTTgccgcca                                                                    >  1:1274364/1‑148 (MQ=255)

                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCATCCACAAAACCTGTTTtccgcca                                                                    >  1:4253364/1‑148 (MQ=255)

                                                                                  cgCCCGCTTTGCGACCACGGGAGTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                    <  2:1921176/148‑1 (MQ=255)

                                                                                                  aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTTCGCAGACTCAAAACAGCGAAATGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGACGCCTGTCAGCATCTTCTGGc                                                    >  1:2803321/1‑149 (MQ=255)

                                                                                                    gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACACAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGGTTGCCGCCAGTCAGCAACTGCTGGCGa                                                  >  2:3278020/1‑149 (MQ=255)

                                                                                                       aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                               <  2:2229222/149‑1 (MQ=255)

                                                                                                          gTTTTCTATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                            <  2:4092362/149‑1 (MQ=255)

                                                                                                          gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGttag                                            >  2:4295485/1‑147 (MQ=255)

                                                                                                           ttttCGTTGTGTGTTACCCCCCTCTAGACAGAAAAGGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  1:575890/149‑1 (MQ=255)

                                                                                                                gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                      <  2:3697503/149‑1 (MQ=255)

                                                                                                                gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                      >  2:213217/1‑149 (MQ=255)

                                                                                                                 aggtatGTTTCCTCCCACCAGTCAGAAAATGCGCATCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCACACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGGTGCGGt                                     <  2:1081726/144‑1 (MQ=255)

                                                                                                                        tttCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGGTTGCCGCCTGTCAGCATCTGCTTGCGATGTTCGTTGCGGTGATCACg                              >  2:4063536/1‑149 (MQ=255)

                                                                                                                        tttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTACGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATAACg                              >  2:1847064/1‑149 (MQ=255)

                                                                                                                            cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                          >  2:4282156/1‑149 (MQ=255)

                                                                                                                                cTCGAGTCATAAATGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt                      <  1:1343247/149‑1 (MQ=255)

                                                                                                                                agcgtcacAGAATATGCGCACCCGGTCCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCt                      <  1:3623019/142‑1 (MQ=255)

                                                                                                                                 tCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                     >  2:2359768/1‑149 (MQ=255)

                                                                                                                                   gAGTCAGCAAAGGCGCACCCGGTTGCCCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  2:742415/149‑1 (MQ=255)

                                                                                                                                   gAGTAATAAAATGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  1:1625689/149‑1 (MQ=255)

                                                                                                                                      taaGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  2:1264786/147‑1 (MQ=255)

                                                                                                                                      tCATAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  2:2007509/149‑1 (MQ=255)

                                                                                                                                        aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGGCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              >  2:3394464/1‑149 (MQ=255)

                                                                                                                                        aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCATTAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCCGCGCAATTAATCt              >  2:2347416/1‑149 (MQ=255)

                                                                                                                                         gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCATGAAGATCGTCCGCCGCATCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             >  2:1296785/1‑149 (MQ=255)

                                                                                                                                          aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc            <  2:706303/149‑1 (MQ=255)

                                                                                                                                           aaaTGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  1:2017878/149‑1 (MQ=255)

                                                                                                                                           aaaTGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  1:1000830/1‑149 (MQ=255)

                                                                                                                                            aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  1:1585985/149‑1 (MQ=255)

                                                                                                                                            aaTGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCCCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATAACGCCAGCGCTATTAATCTGCCa          >  1:1078019/1‑149 (MQ=255)

                                                                                                                                               gcgcACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc       <  2:1342720/149‑1 (MQ=255)

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`