Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 698,274 C→T 14.2% G265D (GGT→GAT)  nagB ← glucosamine‑6‑phosphate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273698,2740CT14.2% 676.4 / 84.0 233G265D (GGT→GAT) nagBglucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base C (91/109);  new base T (15/18);  total (106/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 242 total aligned reads displayed.

ATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACG  >  CP009273/698125‑698423
                                                                                                                                                     |                                                                                                                                                     
aTCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGAc                                                                                                                                                       >  2:683309/1‑150 (MQ=255)
aTCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGAc                                                                                                                                                       >  1:709482/1‑150 (MQ=255)
 tCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGAcc                                                                                                                                                      <  1:989404/150‑1 (MQ=255)
  cAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGAATTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCt                                                                                                                                                     >  2:1976461/1‑150 (MQ=255)
   aGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGCCCtt                                                                                                                                                    <  2:2717231/150‑1 (MQ=255)
   aGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCtt                                                                                                                                                    <  1:1016304/150‑1 (MQ=255)
   aGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCtt                                                                                                                                                    >  1:3311239/1‑150 (MQ=255)
   aGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCtt                                                                                                                                                    <  1:2318314/150‑1 (MQ=255)
       cATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGat                                                                                                                                                <  2:1910702/150‑1 (MQ=255)
        aTCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGata                                                                                                                                               <  1:1529990/150‑1 (MQ=255)
        aTCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGata                                                                                                                                               <  1:3679939/150‑1 (MQ=255)
           aGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATGCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACACTTACAGACCTTTGATAttt                                                                                                                                            >  2:124283/1‑150 (MQ=255)
                 aacaacCGCGTGGTCATCAAGTAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCt                                                                                                                                      <  2:912752/150‑1 (MQ=255)
                 aacaacCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCt                                                                                                                                      <  2:743479/150‑1 (MQ=255)
                  acaacCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCtt                                                                                                                                     <  1:1085814/150‑1 (MQ=255)
                    aacCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCt                                                                                                                                   >  1:2602374/1‑150 (MQ=255)
                       cgcgTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAAt                                                                                                                                <  1:406523/150‑1 (MQ=255)
                               catcaAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGaa                                                                                                                        <  1:3775801/150‑1 (MQ=255)
                                atcaAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGaaa                                                                                                                       <  2:4540552/150‑1 (MQ=255)
                                  caAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAata                                                                                                                     >  1:2489764/1‑150 (MQ=255)
                                  caAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAata                                                                                                                     <  2:1070835/150‑1 (MQ=255)
                                   aaGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAatat                                                                                                                    <  2:3789014/150‑1 (MQ=255)
                                    aGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATc                                                                                                                   >  1:4476256/1‑150 (MQ=255)
                                      aaaTTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGTAATATCtt                                                                                                                 >  2:3408750/1‑150 (MQ=255)
                                       aaTTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTa                                                                                                                <  1:3559324/150‑1 (MQ=255)
                                        aTTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTaa                                                                                                               <  2:1376013/150‑1 (MQ=255)
                                                ccGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTaa                                                                                                       <  1:2057018/150‑1 (MQ=255)
                                                 cGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAAc                                                                                                      <  2:2580098/150‑1 (MQ=255)
                                                  ggTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACt                                                                                                     >  1:189686/1‑150 (MQ=255)
                                                   gTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACtt                                                                                                    <  2:3436790/150‑1 (MQ=255)
                                                    tAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACttt                                                                                                   <  1:3286739/150‑1 (MQ=255)
                                                    tAAAGATCCGGCCCTGGGTTAATGCATACAGTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACttt                                                                                                   <  2:2443016/150‑1 (MQ=255)
                                                      aaGATCCGGCCCTGGGTTAATGCATACAGTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAAGTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCa                                                                                                 <  2:3915317/150‑1 (MQ=255)
                                                        gATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGc                                                                                               <  2:3979645/150‑1 (MQ=255)
                                                         aTCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCt                                                                                              <  1:4038507/150‑1 (MQ=255)
                                                            cGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCa                                                                                           <  2:4231379/150‑1 (MQ=255)
                                                              gCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtg                                                                                         <  2:1562966/150‑1 (MQ=255)
                                                              gCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtg                                                                                         >  1:891307/1‑150 (MQ=255)
                                                                ccTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtggt                                                                                       >  1:202225/1‑150 (MQ=255)
                                                                 cagggTTAATGCATACAGTCTGACCCCCGATTTCAAAAAATATTGCCCTGAGCAAGGAGCCATGGCAGTGATAACAATTACAGACATTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTACAtggtg                                                                                      <  1:1665749/148‑1 (MQ=255)
                                                                 cTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtggtg                                                                                      <  1:59950/150‑1 (MQ=255)
                                                                  tGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtggtgg                                                                                     >  2:4095784/1‑150 (MQ=255)
                                                                  tGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtggtgg                                                                                     >  1:214500/1‑150 (MQ=255)
                                                                  tGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtggtgg                                                                                     <  1:465982/150‑1 (MQ=255)
                                                                  tGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCAtggtgg                                                                                     >  2:678702/1‑150 (MQ=255)
                                                                    ggTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGaa                                                                                   <  2:279071/150‑1 (MQ=255)
                                                                     gTTAATTCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAg                                                                                  <  2:1385826/150‑1 (MQ=255)
                                                                     gTTAATGCATACATTCTGACCACCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAg                                                                                  >  2:2343776/1‑150 (MQ=255)
                                                                        aaTGCATACATTCTGACCCCCGATTTTAAAAAATAGTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGtt                                                                               <  2:4285651/150‑1 (MQ=255)
                                                                         aTGCATACATTCTGACCCCCGATTTTAAAAAATAGTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTc                                                                              <  2:2303881/150‑1 (MQ=255)
                                                                           gCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCAt                                                                            <  2:2145297/150‑1 (MQ=255)
                                                                             aTACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGACTTAACTGTCAGCTCCATGGTGTAAGGTTCAtag                                                                          >  1:1170937/1‑148 (MQ=255)
                                                                             aTACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAAGTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCg                                                                          <  2:2388417/150‑1 (MQ=255)
                                                                              tACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGc                                                                         >  1:2551950/1‑150 (MQ=255)
                                                                                cATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGcac                                                                       >  2:1632642/1‑150 (MQ=255)
                                                                                  ttCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCacac                                                                     <  1:2800819/150‑1 (MQ=255)
                                                                                      gACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATg                                                                 <  2:1445477/150‑1 (MQ=255)
                                                                                       aCCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGa                                                                <  2:3133391/150‑1 (MQ=255)
                                                                                        cccccGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGAt                                                               <  2:2867518/150‑1 (MQ=255)
                                                                                        cccccGATTTTAAAAAATAGTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGAt                                                               <  2:4193890/150‑1 (MQ=255)
                                                                                         ccccGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATc                                                              >  2:2929093/1‑150 (MQ=255)
                                                                                            cgcttttAAAATATATTGCCCTGAGCAAGGAGCGAGGGCTGGGATTTCCATTTCTGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTATAGTCTTAACTTTCAGCGCGATGGGGGAAGGTTGATCGCAGACCATGATCGCt                                                           <  2:3522816/147‑1 (MQ=255)
                                                                                            cGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCt                                                           <  2:3607177/150‑1 (MQ=255)
                                                                                            cGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCt                                                           <  2:2639914/150‑1 (MQ=255)
                                                                                            cGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCt                                                           <  2:3607004/150‑1 (MQ=255)
                                                                                             gATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCtt                                                          <  1:2736302/150‑1 (MQ=255)
                                                                                                 ttAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCgg                                                      <  1:1767866/150‑1 (MQ=255)
                                                                                                   aaaaaaTATTTCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGAt                                                    <  1:1854593/150‑1 (MQ=255)
                                                                                                   aaaaaaTATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGAt                                                    >  2:889512/1‑150 (MQ=255)
                                                                                                   aaaaaaTATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGAt                                                    >  2:587853/1‑150 (MQ=255)
                                                                                                    aaaaaTATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATg                                                   >  1:3763318/1‑150 (MQ=255)
                                                                                                        atatTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGt                                               <  2:724714/150‑1 (MQ=255)
                                                                                                         tatTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGtt                                              >  2:1539361/1‑150 (MQ=255)
                                                                                                         tatTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGtt                                              <  2:4427487/150‑1 (MQ=255)
                                                                                                         tatTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGtt                                              <  1:1976518/150‑1 (MQ=255)
                                                                                                          atTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTg                                             >  2:2530929/1‑150 (MQ=255)
                                                                                                               ccTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGAc                                        <  1:1616428/150‑1 (MQ=255)
                                                                                                               ccTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGAc                                        >  1:4026414/1‑150 (MQ=255)
                                                                                                               ccTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGAc                                        >  1:522847/1‑150 (MQ=255)
                                                                                                               ccTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGAc                                        <  1:4214370/150‑1 (MQ=255)
                                                                                                                 tGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAg                                      <  1:3106348/150‑1 (MQ=255)
                                                                                                                   aGCAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCt                                    >  2:620860/1‑150 (MQ=255)
                                                                                                                   aGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCt                                    >  1:2941099/1‑150 (MQ=255)
                                                                                                                   aGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCt                                    <  1:1894691/150‑1 (MQ=255)
                                                                                                                   aGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCt                                    >  1:183584/1‑150 (MQ=255)
                                                                                                                     cAAGGAGCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGa                                  <  2:3068979/150‑1 (MQ=255)
                                                                                                                      aaGGAGCCAGGGCAGGTATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGAt                                 <  2:1824033/150‑1 (MQ=255)
                                                                                                                        ggAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATgg                               <  1:1975208/150‑1 (MQ=255)
                                                                                                                           gCCAGGGCAGGGATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTcc                            <  1:3843503/150‑1 (MQ=255)
                                                                                                                             cagggcagggATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCcac                          >  1:879708/1‑150 (MQ=255)
                                                                                                                              agggcagggATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTACAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTATAGGATGCAGTTGCAGACAGCTGATGGTCcaca                         >  1:2769523/1‑150 (MQ=255)
                                                                                                                               gggcagggATAACAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACat                        >  1:649540/1‑150 (MQ=255)
                                                                                                                               gggcagggATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACat                        >  1:3498023/1‑150 (MQ=255)
                                                                                                                                         aaCAATTACAGATCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACg              >  1:1490524/1‑150 (MQ=255)
                                                                                                                                           cAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCa            >  2:3016859/1‑150 (MQ=255)
                                                                                                                                            aaTTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCaa           >  1:755145/1‑150 (MQ=255)
                                                                                                                                                aCAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCtt       <  1:3024708/150‑1 (MQ=255)
                                                                                                                                                aCAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCtt       <  2:3303567/150‑1 (MQ=255)
                                                                                                                                                 cAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTc      <  1:2759245/150‑1 (MQ=255)
                                                                                                                                                  aGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCa     >  1:2453011/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAATGCATACATTCTGACCCCCGATTTTAAAAAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACG  >  CP009273/698125‑698423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑