Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,078 C→G 7.4% intergenic (+34/‑171) ybhL → / → ybhM UPF0005 family inner membrane protein/UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,0780CG 7.4% 778.5 / 2.6 267intergenic (+34/‑171)ybhL/ybhMUPF0005 family inner membrane protein/UPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (102/138);  new base G (3/22);  total (107/160)
Fisher's exact test for biased strand distribution p-value = 2.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTT  >  CP009273/815929‑816227
                                                                                                                                                      |                                                                                                                                                     
aTACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTc                                                                                                                                                       >  2:4064094/1‑150 (MQ=255)
aTACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTc                                                                                                                                                       <  2:4386383/150‑1 (MQ=255)
   cccGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCggg                                                                                                                                                    <  2:3343928/150‑1 (MQ=255)
    ccGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGa                                                                                                                                                   <  1:1336534/150‑1 (MQ=255)
       cgACACGTCGAACCTGCGCAAATAGTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGAttt                                                                                                                                                <  2:3697299/150‑1 (MQ=255)
         acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGGttttt                                                                                                                                              >  2:2616925/1‑150 (MQ=255)
         acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGAttttt                                                                                                                                              <  2:120372/150‑1 (MQ=255)
             gTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTGAATTATTTTTAACA‑ATCACTAAACCCGCCATGTGTCGGGAtttttttaa                                                                                                                                          >  1:1908702/1‑148 (MQ=255)
               cGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑CTCACTATCCCGGCCATGTGTCGGGCTTTTTTTGTTa                                                                                                                                        >  1:430205/1‑150 (MQ=255)
                gAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTAc                                                                                                                                       >  1:3666611/1‑150 (MQ=255)
                gAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTAc                                                                                                                                       <  2:57674/150‑1 (MQ=255)
                 caCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACt                                                                                                                                      <  2:4255589/149‑1 (MQ=255)
                  aCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTa                                                                                                                                     <  2:1151061/150‑1 (MQ=255)
                  aCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTa                                                                                                                                     <  1:3086865/150‑1 (MQ=255)
                     tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaa                                                                                                                                  >  2:3915310/1‑150 (MQ=255)
                     tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaa                                                                                                                                  <  2:663334/150‑1 (MQ=255)
                      gcgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaaa                                                                                                                                 <  1:4066619/150‑1 (MQ=255)
                      gcgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaaa                                                                                                                                 <  1:1462336/150‑1 (MQ=255)
                       cgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAc                                                                                                                                >  2:3222955/1‑150 (MQ=255)
                       cgcAAATATTCCATTCTTGGCGCGTTAACCTTGGATCTGGACTTCATCAACCTGTTCCTGATGGTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCCTGTGTCGGGCTTTTTTTCTTACTAAAAAc                                                                                                                                >  2:2345023/1‑150 (MQ=255)
                        gcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACg                                                                                                                               >  2:2843119/1‑150 (MQ=255)
                        gcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACAAAAAACg                                                                                                                               >  2:828423/1‑150 (MQ=255)
                               ttCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTAACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTGTTAATAAAAAAGAAACATa                                                                                                                        >  1:4233499/1‑150 (MQ=255)
                                  cATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTACGGCAACCGCCGTTAATTCTTTTTAACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACa                                                                                                                     >  2:1940889/1‑150 (MQ=255)
                                   aTTCTTGGCGCTTTACCCTTGAATTTGTACTTCATCACACTGTTCCTGATGTTGTTGCGGAAATTCGGCAACCGCCGTTAATTATTTTTCACA‑ATCACTATCCCTGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACaa                                                                                                                    <  2:3897613/150‑1 (MQ=255)
                                   aTTCTTGGCGCTTTAACCTTGTATATGGACTTAATCAACCTGTTCCTTATGTTGTTGCGTATCTTCGGCAACCGCCGTTAATTATTTTACACA‑ATCACTATCCCGGCAATTTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACaa                                                                                                                    <  2:1616803/150‑1 (MQ=255)
                                    ttCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACaat                                                                                                                   >  2:557020/1‑149 (MQ=255)
                                         ggCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGTTGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCAATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCa                                                                                                              <  1:1872358/150‑1 (MQ=255)
                                         ggCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCa                                                                                                              >  1:4130599/1‑150 (MQ=255)
                                          gcgcTTTAACCTTTTTTCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCGTTAATTATTTTTAACC‑ATCAATATCCCGGCCAGGGGTCGGTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAg                                                                                                             <  1:290978/150‑1 (MQ=255)
                                           cgggTTAACCTTGTATCTGTACTACATCTCCGTTTTCCTTATGTTTTTGGGGATGTTCGGAAACCGCCTTTTATTCTTTTTAACA‑ATCACTATCCCGGCATTGTGTGGGTTTTTTTTTGTTTATAAAAACGACACTTAACAAGTACCAgg                                                                                                            <  2:4306455/147‑1 (MQ=255)
                                               ttAACCTTTTATCTGGACTTCATCAACCTGTTCCTGTTGTTGTTGCGGTTATTCGGAAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCAATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGtata                                                                                                        <  2:1573364/150‑1 (MQ=255)
                                                         aTCTGGACTTCATCACCCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGGGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCt                                                                                              <  2:3690004/150‑1 (MQ=255)
                                                              gACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATgg                                                                                         >  2:215242/1‑150 (MQ=255)
                                                               aCTTCATCAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCAGGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGa                                                                                        <  2:934137/150‑1 (MQ=255)
                                                               aCTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCTTTAATTCTTTTTCACC‑ATCACTATCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGa                                                                                        <  1:3691455/150‑1 (MQ=255)
                                                                cTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGAc                                                                                       >  1:2443676/1‑150 (MQ=255)
                                                                cTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGAc                                                                                       >  2:2875430/1‑150 (MQ=255)
                                                                   catcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTAATTTTAACC‑AACACATTCCCGGCCAAGTGTCGGGATTTTTTTGTTAATAAAAACGACAAATAACAAGTAACAGGGATAAAAAATGCCTTATTGACGAg                                                                                    >  1:16315/1‑150 (MQ=255)
                                                                   catcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAAATATTTTTAACC‑CACACAAAACCCGCCAAGTGTCGGGCTTTTTTTCTTACTAAAAAAGACACATAAAAAGTAACCGGTATAAAAAATGCCATATTGACCAg                                                                                    >  1:2283209/1‑150 (MQ=255)
                                                                    atcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGTTTTTTTTCTTACTAAAAACGAAACTTAACAAGTACCAGGTATAAAATATGCCTTATTGACGAGc                                                                                   >  2:3098142/1‑150 (MQ=255)
                                                                     tcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCACCCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCg                                                                                  <  2:1742400/150‑1 (MQ=255)
                                                                      caACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCgg                                                                                 <  2:2885952/150‑1 (MQ=255)
                                                                      caACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAAAATTCCTTATGGACGAGCgg                                                                                 >  1:3366251/1‑150 (MQ=255)
                                                                       aaCCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATACCGGCCATGTGTGGGGATTTTTTTCTTACTAAAAAAGACACATAACAAGTACCAGGTATAAAATATGCCATATGGACGAGCGGa                                                                                >  1:4395924/1‑150 (MQ=255)
                                                                       aaCCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTAACC‑ATCACAAAACCGGCCATGTGTCGGGCTTTTTTTGTTACTAAAAAAGACACTTAACAAATAACAGGTATAAAATATTCCATATGGACGAGCGGa                                                                                >  1:2779391/1‑150 (MQ=255)
                                                                        aCCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGaa                                                                               >  1:760315/1‑150 (MQ=255)
                                                                           tGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCg                                                                            >  2:2822187/1‑150 (MQ=255)
                                                                           tGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGAAACTTAACAAGTACCAGGTATAAAATATTCCTTATTGACGAGCGGAAGCg                                                                            >  2:2469846/1‑150 (MQ=255)
                                                                           tGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTAACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCg                                                                            >  1:3713571/1‑150 (MQ=255)
                                                                            gTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGt                                                                           <  2:2984937/150‑1 (MQ=255)
                                                                              tCCTGTTGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTACACC‑ATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGttt                                                                         <  1:1030984/150‑1 (MQ=255)
                                                                                cTGATGTTGTTGCGGATCTACGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTc                                                                       <  2:750486/150‑1 (MQ=255)
                                                                                 tGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCCCC‑ATCACTAGCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCt                                                                      <  2:3840817/150‑1 (MQ=255)
                                                                                  gATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATTCATTATGGACGAGCGGAAGCGTTTTCTg                                                                     >  2:135260/1‑150 (MQ=255)
                                                                                          tgCGGATCTTCGGCAACCGCCGTTAAATCTTTTTCACC‑ATCACTATCCCGGCCATGTGTAGGGATTTTTTTCTTACTAAAAACGACAATTAACAAGTAACAGGTATAAAATATTCCATATTGACGAGCGGAAGCGTTTTATTTATCCaaa                                                             >  1:2608091/1‑148 (MQ=255)
                                                                                           gCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATaa                                                            <  2:627924/150‑1 (MQ=255)
                                                                                            cGGATCTTCGGCACCCGCCTTTAATTCTTTTTCACC‑ACCACTAGCCCGGCCTTGTGTCGGTTTTTTTTACTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATaaa                                                           <  1:2432552/150‑1 (MQ=255)
                                                                                             ggATCTTCGGCAACCGCCTTTATTTTTTTTTCACC‑ATCACTAGCCCGGCCATGTGGCGGTATTTTTTTCTTAATAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAAt                                                          <  1:490841/150‑1 (MQ=255)
                                                                                             ggATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAAt                                                          <  2:2233682/150‑1 (MQ=255)
                                                                                              gATATTCGGAAACCGCCTTTAATTCTTTTTCACC‑AACAATATCCCGGCATTGGGTGGGTTTTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATg                                                         <  1:1251334/150‑1 (MQ=255)
                                                                                                     gccAACCGCCTTTAATTC‑TTTTCACCAATCACTCTCCCGGCCTTGTTTGTGTTTTTTTTTCTTAATAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGt                                                  <  1:3043894/148‑1 (MQ=255)
                                                                                                       cAACCGCCGTTAATTCTTTTTCACC‑CTCACAATCCCCGCCATGTGTCGGGATTTTTTTATTACTAAAAAAGACACATAAAAAGTACCAGGTATAAAATATTACTTATTGACGAGCGGAAGCGTTTTAAGTATACAAAAAAGCAAAAGTaa                                                >  1:544096/1‑149 (MQ=255)
                                                                                                       cAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATTGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAt                                                >  2:3607922/1‑150 (MQ=255)
                                                                                                         aCCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAttt                                              <  2:2019341/150‑1 (MQ=255)
                                                                                                          ccgccgTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAtttt                                             <  2:101020/150‑1 (MQ=255)
                                                                                                          ccgccgTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAtttt                                             <  2:2192399/150‑1 (MQ=255)
                                                                                                           cgccgTTAATTCTTTTTCACA‑ATCACTATCCCGGCCATGTGTGGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTg                                            <  1:1781306/150‑1 (MQ=255)
                                                                                                             ccgTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTa                                          <  2:1417916/150‑1 (MQ=255)
                                                                                                             ccgTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTa                                          <  2:379670/150‑1 (MQ=255)
                                                                                                              ctttAATTCTTTTACACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTAt                                         <  1:2932364/148‑1 (MQ=255)
                                                                                                              cgTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTAt                                         >  1:2214155/1‑150 (MQ=255)
                                                                                                                 tAATTCTTTTTCCCC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCCGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCg                                      <  2:376843/150‑1 (MQ=255)
                                                                                                                 tAATTCTTTTTCACC‑AACACTATCCCGGCCATGTGTGGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCg                                      <  2:1899727/150‑1 (MQ=255)
                                                                                                                 tAATACTTTTCCACC‑AACACTATCCCGCCATTGTTTCGGCATTTTTTTATTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCg                                      <  2:2650031/150‑1 (MQ=255)
                                                                                                                   aTTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTg                                    <  2:4420780/150‑1 (MQ=255)
                                                                                                                      cTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGtt                                 >  1:1463473/1‑150 (MQ=255)
                                                                                                                       tttttCACC‑ATCACTATCCCGGCCATGTGTCGGTTTTTTTTTATTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGttt                                <  1:392982/150‑1 (MQ=255)
                                                                                                                            catc‑ATCACTCGCCCGGCCTGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAt                           <  2:813593/147‑1 (MQ=255)
                                                                                                                            cACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAt                           >  1:2141508/1‑150 (MQ=255)
                                                                                                                            cACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAt                           >  1:4454838/1‑150 (MQ=255)
                                                                                                                            cACC‑AACACTATCCCGCCCAGGGGTGGGTTTTTTTTTCTAAAAAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAt                           <  1:834009/150‑1 (MQ=255)
                                                                                                                             aCC‑ATCACTAGCCCTGCCATGTGTCGTGATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAtt                          <  2:4262933/150‑1 (MQ=255)
                                                                                                                              cc‑ATCACTATCCCGGCCCTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAttt                         <  1:593139/150‑1 (MQ=255)
                                                                                                                               c‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTa                        >  2:3625476/1‑150 (MQ=255)
                                                                                                                               c‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTa                        >  1:365867/1‑150 (MQ=255)
                                                                                                                                 aTCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTTAATTTAt                       >  1:3512116/1‑150 (MQ=255)
                                                                                                                                      taccccGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATg                  <  2:3583219/147‑1 (MQ=255)
                                                                                                                                      tATCCCGGCCATGTGTCGGGATTGTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCACAAGTATTTGGTATGCGGGGTTTTAAATTTATTGATg                  >  1:651809/1‑150 (MQ=255)
                                                                                                                                      tATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATCTGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATg                  <  2:1839959/150‑1 (MQ=255)
                                                                                                                                       aTCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATgg                 <  2:2417164/150‑1 (MQ=255)
                                                                                                                                         cccGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTg               <  2:2607973/150‑1 (MQ=255)
                                                                                                                                             gCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaata           >  2:1223800/1‑150 (MQ=255)
                                                                                                                                             gCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaata           <  2:1094621/150‑1 (MQ=255)
                                                                                                                                              ccttgtgTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatat          <  2:3224181/147‑1 (MQ=255)
                                                                                                                                               cctgtgTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatt         <  1:3836476/148‑1 (MQ=255)
                                                                                                                                               cATGTGTCGGTATTTTTTTCTAACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatt         <  2:4233415/150‑1 (MQ=255)
                                                                                                                                                 tgtgTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCAGTATCCAAAAATTCAAAAGAATTTTGTTTGCGTGGTTTTAAAATTATTTATGTTGaaaaataa       >  1:2661203/1‑150 (MQ=255)
                                                                                                                                                  gggtCGGGATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaat      <  2:4486946/148‑1 (MQ=255)
                                                                                                                                                  gggtCGGGAATTTTTTCTTCCTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaat      <  2:4486845/148‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACC‑ATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATTT  >  CP009273/815929‑816227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑