Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,152,278 G→T 86.5% E16* (GAA→TAA)  ycfH → putative DNase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,152,2780GT86.5% 683.7 / 102.2 259E16* (GAA→TAA) ycfHputative DNase
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base T (117/107);  total (137/122)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

AGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGG  >  CP009273/1152129‑1152426
                                                                                                                                                     |                                                                                                                                                    
   cATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAAt                                                                                                                                                   <  1:3882003/150‑1 (MQ=255)
    aTCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATc                                                                                                                                                  >  2:1196849/1‑150 (MQ=255)
     tCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCt                                                                                                                                                 >  1:2629699/1‑150 (MQ=255)
     tCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCt                                                                                                                                                 >  2:3232737/1‑150 (MQ=255)
      cAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGAGGGTCTGGATTATTAATCtt                                                                                                                                                <  2:655409/150‑1 (MQ=255)
          cgcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCa                                                                                                                                            >  2:842796/1‑150 (MQ=255)
          cgcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCa                                                                                                                                            <  1:169934/150‑1 (MQ=255)
              aGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAg                                                                                                                                        >  1:1520783/1‑150 (MQ=255)
              aGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAg                                                                                                                                        <  1:3759980/150‑1 (MQ=255)
                cTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGa                                                                                                                                      <  1:3689775/150‑1 (MQ=255)
                   ctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGt                                                                                                                                   >  1:2036027/1‑150 (MQ=255)
                      atcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTATGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGa                                                                                                                                <  2:2069231/150‑1 (MQ=255)
                       tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGAt                                                                                                                               <  1:1289622/150‑1 (MQ=255)
                       tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGAt                                                                                                                               <  1:1956192/150‑1 (MQ=255)
                        caCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATg                                                                                                                              >  2:2448244/1‑150 (MQ=255)
                         aCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGa                                                                                                                             <  2:558993/150‑1 (MQ=255)
                         aCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGa                                                                                                                             >  2:94837/1‑150 (MQ=255)
                           cGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACg                                                                                                                           <  2:2108966/150‑1 (MQ=255)
                              tCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTc                                                                                                                        >  2:344526/1‑150 (MQ=255)
                                ttttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTg                                                                                                                      <  2:4397974/150‑1 (MQ=255)
                                    aCTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCACATCTTTAAGAGAGACATCATGTTTTTAGTAGTCACACACTGCCATCTCGATGGTCTGGATTATTTATCTTTTCATAAAGACGTGGATGACGTTATTGCGa                                                                                                                  >  1:3858977/1‑150 (MQ=255)
                                     cTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAGAAGGACGTGGATGACGTTCTGGCGaa                                                                                                                 >  2:237239/1‑150 (MQ=255)
                                             tGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGccgccg                                                                                                         >  2:3007726/1‑150 (MQ=255)
                                              gAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCCTGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCcgccgc                                                                                                        >  2:1906232/1‑150 (MQ=255)
                                              gAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCcgccgc                                                                                                        >  2:560323/1‑150 (MQ=255)
                                               aGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCa                                                                                                       <  1:1851123/150‑1 (MQ=255)
                                                  aTTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAcgc                                                                                                    >  2:175258/1‑150 (MQ=255)
                                                    tACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGa                                                                                                  >  1:2469752/1‑150 (MQ=255)
                                                     aCCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAt                                                                                                 >  2:166574/1‑150 (MQ=255)
                                                      ccTGCAACCGGGCGTTGTGCGACCGGGTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtg                                                                                                <  1:1591101/150‑1 (MQ=255)
                                                          cAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaa                                                                                            >  1:563733/1‑150 (MQ=255)
                                                            aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTATTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATATTTTCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAt                                                                                          >  1:1925272/1‑150 (MQ=255)
                                                            aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAt                                                                                          <  1:4505104/150‑1 (MQ=255)
                                                            aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAt                                                                                          >  1:1782233/1‑150 (MQ=255)
                                                            aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAt                                                                                          >  2:3822479/1‑150 (MQ=255)
                                                             ccGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtt                                                                                         >  2:2396107/1‑150 (MQ=255)
                                                              cGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAttt                                                                                        >  1:2203028/1‑150 (MQ=255)
                                                              cGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAttt                                                                                        >  1:2273040/1‑150 (MQ=255)
                                                              cGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAttt                                                                                        >  1:1332406/1‑150 (MQ=255)
                                                               gggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtttt                                                                                       >  1:3268385/1‑150 (MQ=255)
                                                                ggCGTTGTGCTACCGTTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACGGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTg                                                                                      <  2:810206/150‑1 (MQ=255)
                                                                       tgCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCa                                                                               >  2:1578742/1‑150 (MQ=255)
                                                                        gCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAg                                                                              <  2:2558290/150‑1 (MQ=255)
                                                                           aCCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCg                                                                           <  1:1022274/150‑1 (MQ=255)
                                                                               gTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcac                                                                       >  1:2485090/1‑150 (MQ=255)
                                                                                   cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                   <  1:3138228/150‑1 (MQ=255)
                                                                                   cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                   <  2:1341128/150‑1 (MQ=255)
                                                                                     cATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAtt                                                                 >  2:4278141/1‑150 (MQ=255)
                                                                                      aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTa                                                                <  2:2146576/150‑1 (MQ=255)
                                                                                      aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTa                                                                <  2:2004576/150‑1 (MQ=255)
                                                                                        cTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAcc                                                              <  1:145195/150‑1 (MQ=255)
                                                                                           tAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCggg                                                           <  2:4309139/150‑1 (MQ=255)
                                                                                             agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         >  2:3170247/1‑150 (MQ=255)
                                                                                             agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         >  2:183967/1‑150 (MQ=255)
                                                                                              gagagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTa                                                        >  1:3378514/1‑150 (MQ=255)
                                                                                                gagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAtt                                                      <  1:2448244/150‑1 (MQ=255)
                                                                                                  gaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTa                                                    >  2:1634596/1‑150 (MQ=255)
                                                                                                  gaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTa                                                    <  1:781054/150‑1 (MQ=255)
                                                                                                   aCATCATTTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTAc                                                   <  2:3521153/150‑1 (MQ=255)
                                                                                                    catcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACa                                                  <  2:258791/150‑1 (MQ=255)
                                                                                                    catcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACa                                                  >  1:4200381/1‑150 (MQ=255)
                                                                                                    catcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACa                                                  >  1:2865455/1‑150 (MQ=255)
                                                                                                      tcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACata                                                >  2:1178942/1‑150 (MQ=255)
                                                                                                      tcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACata                                                <  2:3732426/150‑1 (MQ=255)
                                                                                                           tttttAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCgg                                           >  2:315775/1‑150 (MQ=255)
                                                                                                           tttttAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCgg                                           <  2:3999795/150‑1 (MQ=255)
                                                                                                             tttAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGa                                         <  1:6987/150‑1 (MQ=255)
                                                                                                               tAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATc                                       >  1:512767/1‑150 (MQ=255)
                                                                                                                aGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCt                                      >  1:4366750/1‑150 (MQ=255)
                                                                                                                 gTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTg                                     >  1:1423142/1‑150 (MQ=255)
                                                                                                                 gTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTg                                     <  1:1605874/150‑1 (MQ=255)
                                                                                                                 gTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTg                                     >  2:1752462/1‑150 (MQ=255)
                                                                                                                  tCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTgg                                    <  1:1146352/150‑1 (MQ=255)
                                                                                                                  tCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTgg                                    <  1:2717180/150‑1 (MQ=255)
                                                                                                                   cGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGt                                   >  1:2237951/1‑150 (MQ=255)
                                                                                                                    gACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                  >  1:3033129/1‑150 (MQ=255)
                                                                                                                     aCTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAg                                 >  1:3531962/1‑150 (MQ=255)
                                                                                                                     aCTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAg                                 <  2:4206758/150‑1 (MQ=255)
                                                                                                                        cacaCTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                              <  1:878589/150‑1 (MQ=255)
                                                                                                                         acacTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGa                             >  1:422592/1‑150 (MQ=255)
                                                                                                                            cTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACg                          <  1:1982357/150‑1 (MQ=255)
                                                                                                                             tGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGt                         >  1:2555082/1‑150 (MQ=255)
                                                                                                                              gCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTg                        <  2:1616564/150‑1 (MQ=255)
                                                                                                                               ccATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGa                       >  2:409839/1‑150 (MQ=255)
                                                                                                                                cATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGAc                      >  1:4203462/1‑150 (MQ=255)
                                                                                                                                 aTCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACa                     >  2:3365615/1‑150 (MQ=255)
                                                                                                                                    tcGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcg                  <  2:2893216/150‑1 (MQ=255)
                                                                                                                                    tcGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcg                  <  2:49320/150‑1 (MQ=255)
                                                                                                                                     cGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACTTATGCGGGATCTGGTAGGCGAACGTGACAAcgt                 >  1:759372/1‑150 (MQ=255)
                                                                                                                                     cGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgt                 >  1:3779448/1‑150 (MQ=255)
                                                                                                                                      gATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtc                >  1:4128070/1‑150 (MQ=255)
                                                                                                                                        tGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt              <  1:2922100/150‑1 (MQ=255)
                                                                                                                                          gTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAt            >  1:1233426/1‑150 (MQ=255)
                                                                                                                                           tCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtt           <  1:4411239/150‑1 (MQ=255)
                                                                                                                                           tCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtt           <  2:1546645/150‑1 (MQ=255)
                                                                                                                                            cTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAttt          <  2:3908482/150‑1 (MQ=255)
                                                                                                                                              ggATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTc        <  1:3362190/150‑1 (MQ=255)
                                                                                                                                                 ttattaATCTTTGCGTAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtg     <  2:2687445/150‑1 (MQ=255)
                                                                                                                                                  tATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtgt    <  1:3007726/150‑1 (MQ=255)
                                                                                                                                                  tATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtgt    <  1:550067/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGG  >  CP009273/1152129‑1152426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑