BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,170,302	G→T	17.5%	A107E (GCG→GAG)	ycfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,170,302	0	G	T	17.5%	781.0 / 120.9	271	A107E (GCG→GAG)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (127/96); new base T (26/21); total (153/118)
Fisher's exact test for biased strand distribution p-value = 8.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 276 total aligned reads displayed.

TACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATAC  >  CP009273/1170154‑1170449

tACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAgcgc                                                                                                                                                    >  1:3821927/1‑150 (MQ=255)

 aCCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCa                                                                                                                                                   <  1:1463589/150‑1 (MQ=255)

   cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACt                                                                                                                                                 <  2:473256/150‑1 (MQ=255)

   cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACt                                                                                                                                                 >  1:2367971/1‑150 (MQ=255)

    aCGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACtt                                                                                                                                                <  1:3384731/150‑1 (MQ=255)

     cGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTa                                                                                                                                               >  2:4322866/1‑150 (MQ=255)

     cGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTa                                                                                                                                               >  1:4329039/1‑150 (MQ=255)

      gAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTaa                                                                                                                                              >  1:3759241/1‑150 (MQ=255)

       aaaTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAAc                                                                                                                                             >  2:539809/1‑150 (MQ=255)

       aaaTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAAc                                                                                                                                             <  1:3930555/150‑1 (MQ=255)

       aaaTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAAc                                                                                                                                             >  2:3127757/1‑150 (MQ=255)

         aTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgc                                                                                                                                           >  1:469535/1‑150 (MQ=255)

           aGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgcca                                                                                                                                         >  1:4188199/1‑150 (MQ=255)

            gACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgccag                                                                                                                                        <  2:1376660/150‑1 (MQ=255)

                 ttAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACt                                                                                                                                   >  1:3097146/1‑150 (MQ=255)

                   aaCGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGc                                                                                                                                 >  1:4491774/1‑150 (MQ=255)

                     cGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcac                                                                                                                               >  2:4070938/1‑150 (MQ=255)

                        aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcaccac                                                                                                                            <  1:3780016/150‑1 (MQ=255)

                        aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcaccac                                                                                                                            >  2:4483974/1‑150 (MQ=255)

                        aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcaccac                                                                                                                            >  1:189887/1‑150 (MQ=255)

                             gATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGcccc                                                                                                                       >  2:1650574/1‑150 (MQ=255)

                              aTACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCa                                                                                                                      <  1:4451981/150‑1 (MQ=255)

                              aTACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCa                                                                                                                      >  2:2184575/1‑150 (MQ=255)

                              aTACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCa                                                                                                                      >  2:4366296/1‑150 (MQ=255)

                               tACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCaa                                                                                                                     >  2:3719041/1‑150 (MQ=255)

                                aCCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCaaa                                                                                                                    >  2:4226162/1‑150 (MQ=255)

                                 ccACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAg                                                                                                                   <  1:2817006/150‑1 (MQ=255)

                                  cacaAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAgc                                                                                                                  >  1:2411055/1‑150 (MQ=255)

                                   acaAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAgcg                                                                                                                 <  1:205190/150‑1 (MQ=255)

                                    caAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAgcgc                                                                                                                >  2:779929/1‑150 (MQ=255)

                                      aGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCa                                                                                                              >  2:1096506/1‑150 (MQ=255)

                                         tGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCCCTGCACCACGCCCCAAAGCGCCAGCa                                                                                                           >  2:1016860/1‑150 (MQ=255)

                                         tGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAGTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCa                                                                                                           >  2:1016907/1‑150 (MQ=255)

                                          ggTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCAc                                                                                                          >  2:1769309/1‑150 (MQ=255)

                                            tGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCAccc                                                                                                        >  2:3239107/1‑150 (MQ=255)

                                              cTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCag                                                                                                      >  1:2446190/1‑150 (MQ=255)

                                              cTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCag                                                                                                      <  2:2064280/150‑1 (MQ=255)

                                               tGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCaga                                                                                                     >  1:1240171/1‑150 (MQ=255)

                                               tGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCaga                                                                                                     >  2:171593/1‑150 (MQ=255)

                                                 gCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCagaag                                                                                                   >  2:11651/1‑150 (MQ=255)

                                                  cGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCagaaga                                                                                                  <  2:2501359/150‑1 (MQ=255)

                                                  cGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCagaaga                                                                                                  <  1:4032560/150‑1 (MQ=255)

                                                   ggTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGAt                                                                                                 <  1:1425597/150‑1 (MQ=255)

                                                    gTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATg                                                                                                <  1:2082453/150‑1 (MQ=255)

                                                       atcatcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTc                                                                                             <  2:2072461/150‑1 (MQ=255)

                                                        tcatcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTcc                                                                                            >  1:1037644/1‑150 (MQ=255)

                                                           tcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGa                                                                                         <  2:1340316/150‑1 (MQ=255)

                                                                  cAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGttt                                                                                  <  2:377027/150‑1 (MQ=255)

                                                                   aGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTa                                                                                 >  1:581950/1‑150 (MQ=255)

                                                                    ggAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTAt                                                                                >  2:4273367/1‑150 (MQ=255)

                                                                    ggAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTAt                                                                                <  1:3164764/150‑1 (MQ=255)

                                                                     gAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATc                                                                               >  1:1184120/1‑150 (MQ=255)

                                                                       aCTCACCGGTGGAATCGGCATACGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCga                                                                             <  1:1905082/150‑1 (MQ=255)

                                                                           acgtgTGGTATCGGCATAAGCGGCATTGTAGGCATTTCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACa                                                                         <  2:3523802/146‑1 (MQ=255)

                                                                             cGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAAt                                                                       >  1:956450/1‑150 (MQ=255)

                                                                              ggTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAACGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAAtt                                                                      <  1:1607567/150‑1 (MQ=255)

                                                                               gTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAAttt                                                                     <  1:2930610/150‑1 (MQ=255)

                                                                                tGGAATCGGAATAAGCGGAATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTc                                                                    <  1:3565029/150‑1 (MQ=255)

                                                                                 ggAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTcc                                                                   >  2:601082/1‑150 (MQ=255)

                                                                                          aTAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTAc                                                          <  2:2350828/150‑1 (MQ=255)

                                                                                          aTAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTAc                                                          <  2:2341341/150‑1 (MQ=255)

                                                                                          aTAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTAc                                                          >  1:1301175/1‑150 (MQ=255)

                                                                                           tacgCGGCATTGGAGGCTTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACg                                                         <  1:1135226/147‑1 (MQ=255)

                                                                                             aGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCt                                                       <  1:3275838/150‑1 (MQ=255)

                                                                                              gCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTg                                                      <  1:1410384/150‑1 (MQ=255)

                                                                                              gCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTg                                                      <  1:941351/150‑1 (MQ=255)

                                                                                               cGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGt                                                     >  1:808342/1‑150 (MQ=255)

                                                                                                 gCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCg                                                   >  2:1900411/1‑150 (MQ=255)

                                                                                                  cATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGa                                                  <  2:1493058/150‑1 (MQ=255)

                                                                                                   aTTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGat                                                 >  1:3659329/1‑150 (MQ=255)

                                                                                                     tGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGatat                                               >  2:3192438/1‑150 (MQ=255)

                                                                                                      ggAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGAtata                                              >  1:154663/1‑150 (MQ=255)

                                                                                                         ggCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcg                                           <  1:1725504/150‑1 (MQ=255)

                                                                                                            aTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggcg                                        >  2:3301684/1‑150 (MQ=255)

                                                                                                            aTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggcg                                        <  1:3601070/150‑1 (MQ=255)

                                                                                                            aTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggcg                                        >  1:727864/1‑150 (MQ=255)

                                                                                                            aTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggcg                                        >  1:897611/1‑150 (MQ=255)

                                                                                                              tGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggcaca                                      >  2:1791365/1‑147 (MQ=255)

                                                                                                                cTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATc                                    >  2:4056190/1‑150 (MQ=255)

                                                                                                                cTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATc                                    >  1:2095728/1‑150 (MQ=255)

                                                                                                                cTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATc                                    <  1:254637/150‑1 (MQ=255)

                                                                                                                     aTCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATa                               <  2:278643/150‑1 (MQ=255)

                                                                                                                         cgcTCAGGTGTCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCg                           >  2:1546904/1‑150 (MQ=255)

                                                                                                                              aGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGatat                      >  2:2427632/1‑150 (MQ=255)

                                                                                                                                gTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATaa                    <  2:3266929/150‑1 (MQ=255)

                                                                                                                                   ccagccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGa                 >  2:2662922/1‑150 (MQ=255)

                                                                                                                                    cagccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCGGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGaa                >  1:4229120/1‑150 (MQ=255)

                                                                                                                                      gccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGaaaa              >  2:1256165/1‑150 (MQ=255)

                                                                                                                                       ccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGaaaaa             >  2:1103158/1‑150 (MQ=255)

                                                                                                                                         aCTGGTTCAGTGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGaaaaaaa           <  1:4088034/150‑1 (MQ=255)

                                                                                                                                          cTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAAc          <  2:3853751/150‑1 (MQ=255)

                                                                                                                                           tGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAAcc         >  2:3479341/1‑150 (MQ=255)

                                                                                                                                             gTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCgg       >  2:1786675/1‑150 (MQ=255)

                                                                                                                                             gTTCAGCTCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCgg       >  1:1863603/1‑150 (MQ=255)

                                                                                                                                             gTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCgg       <  2:2382644/150‑1 (MQ=255)

                                                                                                                                              ttCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGc      <  2:3804477/150‑1 (MQ=255)

                                                                                                                                              ttCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAACTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGc      <  1:2091876/150‑1 (MQ=255)

                                                                                                                                              ttCAGCACACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGc      <  2:2292080/150‑1 (MQ=255)

                                                                                                                                                 aGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATa   <  2:3095961/150‑1 (MQ=255)

                                                                                                                                                 aGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAAAAGCCGGATATAAAGAAAAAAACCGGCATa   >  1:3047502/1‑150 (MQ=255)

TACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATAC  >  CP009273/1170154‑1170449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`