Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,220,996 G→T 87.5% R181R (CGG→AGG)  minC ← cell division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,220,9960GT87.5% 666.2 / 91.2 247R181R (CGG→AGG) minCcell division inhibitor
Reads supporting (aligned to +/- strand):  ref base G (20/11);  new base T (123/91);  total (144/103)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 247 total aligned reads displayed.

ACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACC  >  CP009273/1220849‑1221144
                                                                                                                                                   |                                                                                                                                                    
aCGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGt                                                                                                                                                    <  2:3051229/150‑1 (MQ=255)
aCGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGt                                                                                                                                                    <  1:960457/150‑1 (MQ=255)
    ttGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTcacc                                                                                                                                                <  1:4072046/150‑1 (MQ=255)
       aaCGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACt                                                                                                                                             >  1:3198895/1‑150 (MQ=255)
         cGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTg                                                                                                                                           <  2:1557923/150‑1 (MQ=255)
             cAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGcccc                                                                                                                                       >  2:3558369/1‑150 (MQ=255)
             cAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGcccc                                                                                                                                       >  2:1761259/1‑150 (MQ=255)
              aaaGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCt                                                                                                                                      >  1:2296737/1‑150 (MQ=255)
               aaGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTg                                                                                                                                     >  1:2323634/1‑150 (MQ=255)
                   gTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAg                                                                                                                                 >  1:90590/1‑150 (MQ=255)
                   gTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAg                                                                                                                                 >  1:3454861/1‑150 (MQ=255)
                   gTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAg                                                                                                                                 >  2:726525/1‑150 (MQ=255)
                         gACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACg                                                                                                                           >  1:2269703/1‑150 (MQ=255)
                         gACTAACTGCAGTCGCGCCGCTGTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACg                                                                                                                           <  2:3775833/150‑1 (MQ=255)
                             aaCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcg                                                                                                                       >  2:3118633/1‑150 (MQ=255)
                              aCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACcgc                                                                                                                      >  1:3827110/1‑150 (MQ=255)
                              aCTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGAGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCACCTGCCAGCGCACGACcgc                                                                                                                      >  2:3203953/1‑150 (MQ=255)
                                tGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCgcgc                                                                                                                    >  2:3953686/1‑150 (MQ=255)
                                 gCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGGTGGACCGCGca                                                                                                                   <  2:1214265/150‑1 (MQ=255)
                                              tttGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGAc                                                                                                      >  1:2372321/1‑150 (MQ=255)
                                              tttGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGAc                                                                                                      >  2:1737272/1‑150 (MQ=255)
                                               ttGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACa                                                                                                     <  1:3149089/150‑1 (MQ=255)
                                                 gCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                   <  2:1461285/150‑1 (MQ=255)
                                                 gCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                   >  1:3976229/1‑150 (MQ=255)
                                                 gCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATg                                                                                                   <  2:1875244/150‑1 (MQ=255)
                                                  ccATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATACACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGa                                                                                                  >  1:3625396/1‑150 (MQ=255)
                                                    aTAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAAt                                                                                                <  1:457104/150‑1 (MQ=255)
                                                       aaaTTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGtt                                                                                             <  1:3099784/150‑1 (MQ=255)
                                                            ctgctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCAt                                                                                        <  2:87767/150‑1 (MQ=255)
                                                             tgctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATc                                                                                       >  1:1646148/1‑150 (MQ=255)
                                                             tgctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATc                                                                                       >  2:1658318/1‑150 (MQ=255)
                                                               ctgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCgg                                                                                     <  1:1473040/150‑1 (MQ=255)
                                                                tgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGc                                                                                    >  1:2252965/1‑150 (MQ=255)
                                                                tgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGc                                                                                    >  1:4003581/1‑150 (MQ=255)
                                                                tgGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGc                                                                                    >  1:458594/1‑150 (MQ=255)
                                                                  ggATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                                  <  2:4001470/150‑1 (MQ=255)
                                                                  ggATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                                  <  1:2437094/150‑1 (MQ=255)
                                                                  ggATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaa                                                                                  <  1:1253876/150‑1 (MQ=255)
                                                                   gATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGcaat                                                                                 <  1:163196/150‑1 (MQ=255)
                                                                        gATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAAtt                                                                            <  1:578511/150‑1 (MQ=255)
                                                                           cACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCgg                                                                         >  1:1187696/1‑150 (MQ=255)
                                                                            aCTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGc                                                                        >  1:710969/1‑150 (MQ=255)
                                                                              tCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                      >  1:1528770/1‑150 (MQ=255)
                                                                              tCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                      >  2:2464634/1‑150 (MQ=255)
                                                                              tCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGccc                                                                      >  1:135602/1‑150 (MQ=255)
                                                                               cAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGcccc                                                                     >  1:2501869/1‑150 (MQ=255)
                                                                                aGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCa                                                                    >  1:114504/1‑150 (MQ=255)
                                                                                 gCCAGTATTCACCTGCGATGGACACCAGGTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAg                                                                   <  2:3946796/150‑1 (MQ=255)
                                                                                    aGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAgcgc                                                                >  1:3004756/1‑150 (MQ=255)
                                                                                     gTATTCACCTGCGATGGACACCAGGTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCt                                                               <  2:1071746/150‑1 (MQ=255)
                                                                                      tATTCACCTGCGATGGACACCAGATCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGACCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGAGCTa                                                              >  2:1811218/1‑150 (MQ=255)
                                                                                         tCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACg                                                           <  2:3759704/150‑1 (MQ=255)
                                                                                          cACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGt                                                          >  2:4322264/1‑150 (MQ=255)
                                                                                           aCCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTg                                                         <  2:76198/150‑1 (MQ=255)
                                                                                             cTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGc                                                       <  1:2070169/150‑1 (MQ=255)
                                                                                              tGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATTTTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCt                                                      >  2:566827/1‑150 (MQ=255)
                                                                                              tGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCt                                                      <  1:3118633/150‑1 (MQ=255)
                                                                                              tGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCt                                                      <  2:2240815/150‑1 (MQ=255)
                                                                                               gCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCtt                                                     <  2:326919/150‑1 (MQ=255)
                                                                                               gCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATAGTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCtt                                                     <  1:445830/150‑1 (MQ=255)
                                                                                                   tGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTaa                                                 >  1:3562767/1‑150 (MQ=255)
                                                                                                   tGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTaa                                                 >  1:4293456/1‑150 (MQ=255)
                                                                                                     gACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACa                                               <  2:2038599/150‑1 (MQ=255)
                                                                                                          cAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAg                                          >  1:3517974/1‑150 (MQ=255)
                                                                                                            gTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGAt                                        >  2:77488/1‑150 (MQ=255)
                                                                                                            gTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGAt                                        >  2:3845119/1‑150 (MQ=255)
                                                                                                            gTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGAt                                        <  1:3921896/150‑1 (MQ=255)
                                                                                                            gTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCTCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGAt                                        >  2:3581431/1‑150 (MQ=255)
                                                                                                            gTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGAt                                        <  1:2915159/150‑1 (MQ=255)
                                                                                                                 gCCATCAGGTTCGTACAAAATATTTGCGTTTCCCCGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACAt                                   <  2:2422412/150‑1 (MQ=255)
                                                                                                                   cATCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtg                                 >  2:2654394/1‑150 (MQ=255)
                                                                                                                     tCAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATtgtg                               >  2:2908342/1‑150 (MQ=255)
                                                                                                                      cAGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTgg                              >  1:339006/1‑150 (MQ=255)
                                                                                                                       aGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGa                             <  1:1063748/150‑1 (MQ=255)
                                                                                                                       aGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGa                             >  2:45007/1‑150 (MQ=255)
                                                                                                                       aGGTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGa                             >  2:42074/1‑150 (MQ=255)
                                                                                                                         gTTCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGc                           >  2:3395387/1‑150 (MQ=255)
                                                                                                                          ttCGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCa                          <  2:538028/150‑1 (MQ=255)
                                                                                                                            cGTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATa                        <  2:576511/150‑1 (MQ=255)
                                                                                                                             gTACAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATaa                       <  1:1975834/150‑1 (MQ=255)
                                                                                                                               aCAAAATATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAAt                     >  1:4134754/1‑150 (MQ=255)
                                                                                                                                 aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                   <  1:690441/150‑1 (MQ=255)
                                                                                                                                 aaaaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATAc                   <  2:3643189/150‑1 (MQ=255)
                                                                                                                                   aaTATTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGc                 <  1:4490737/150‑1 (MQ=255)
                                                                                                                                   aaTATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACTACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTAGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGc                 >  1:2698283/1‑150 (MQ=255)
                                                                                                                                    atatTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCt                >  1:4362918/1‑150 (MQ=255)
                                                                                                                                    atatTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCt                >  2:3003930/1‑150 (MQ=255)
                                                                                                                                     tatTTGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTg               >  1:3762727/1‑150 (MQ=255)
                                                                                                                                       tttGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAc             <  1:2824737/150‑1 (MQ=255)
                                                                                                                                       tttGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAc             <  1:3262459/150‑1 (MQ=255)
                                                                                                                                        ttGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAcc            <  1:357659/150‑1 (MQ=255)
                                                                                                                                        ttGCGTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGAcc            <  2:476772/150‑1 (MQ=255)
                                                                                                                                         tGCGTTTCCCTGTCACGACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCg           >  2:4158613/1‑150 (MQ=255)
                                                                                                                                            gTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa        >  1:2832181/1‑150 (MQ=255)
                                                                                                                                            gTTTCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa        >  1:3333995/1‑150 (MQ=255)
                                                                                                                                            gTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa        >  1:538341/1‑150 (MQ=255)
                                                                                                                                            gTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGaa        >  1:537790/1‑150 (MQ=255)
                                                                                                                                             tttCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAAc       <  1:2401789/150‑1 (MQ=255)
                                                                                                                                               tCCCTGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGc     >  2:1450042/1‑150 (MQ=255)
                                                                                                                                               tCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGc     >  2:4096283/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGGTTGAACGGTCAAAGCGTTTTCGACTAACTGCAGTCGCGCCGCTTTGCCATAAAATTCTGCTGGGATTTGATCACTCAGCCAGTATTCACCTGCGATGGACACCAGTTCCGCCATCAGGTTCGTACAAAATATTTGCGTTTCCCGGTCACCACTTGCCCCTGCCAGCGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACC  >  CP009273/1220849‑1221144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑