BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,301,604	A→T	18.3%	I433I (ATT→ATA)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,301,604	0	A	T	18.3%	641.2 / 131.4	246	I433I (ATT→ATA)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base A (86/115); new base T (15/30); total (101/145)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 251 total aligned reads displayed.

CTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAA  >  CP009273/1301455‑1301753

   aagaagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTAtc                                                                                                                                                    >  2:3930466/1‑150 (MQ=255)

   aagaagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTAtc                                                                                                                                                    <  2:3540970/150‑1 (MQ=255)

      aagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCg                                                                                                                                                 >  1:1412838/1‑150 (MQ=255)

      aagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCg                                                                                                                                                 <  1:2747666/150‑1 (MQ=255)

      aagTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCg                                                                                                                                                 <  1:1853274/150‑1 (MQ=255)

          aaaaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAt                                                                                                                                             >  2:110578/1‑150 (MQ=255)

           aaaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAtt                                                                                                                                            <  2:225946/150‑1 (MQ=255)

           aaaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAtt                                                                                                                                            <  1:385380/150‑1 (MQ=255)

            aaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAttt                                                                                                                                           <  1:4220072/150‑1 (MQ=255)

            aaCAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATATCGAAAttt                                                                                                                                           >  1:1973847/1‑150 (MQ=255)

              cAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAg                                                                                                                                         >  2:115092/1‑150 (MQ=255)

                gTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGcc                                                                                                                                       <  2:3198287/150‑1 (MQ=255)

                gTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGcc                                                                                                                                       <  2:305811/150‑1 (MQ=255)

                    cTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAg                                                                                                                                   <  1:3039920/150‑1 (MQ=255)

                     tCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGa                                                                                                                                  >  1:2060704/1‑150 (MQ=255)

                       ggCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACt                                                                                                                                <  1:372997/150‑1 (MQ=255)

                       ggCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACt                                                                                                                                >  1:261026/1‑150 (MQ=255)

                       ggCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACt                                                                                                                                <  1:372973/150‑1 (MQ=255)

                        gCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTa                                                                                                                               <  1:2522744/150‑1 (MQ=255)

                         cGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTAc                                                                                                                              >  1:1978026/1‑150 (MQ=255)

                          gACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACg                                                                                                                             >  2:4464337/1‑150 (MQ=255)

                           acacGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGc                                                                                                                            >  1:3173810/1‑150 (MQ=255)

                              cGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCata                                                                                                                         >  2:652470/1‑150 (MQ=255)

                               gTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAAACTCGAAATTTAGCCACAGACTACGCatat                                                                                                                        >  2:1022449/1‑150 (MQ=255)

                                     aTAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGt                                                                                                                  <  1:497777/150‑1 (MQ=255)

                                      tAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTATACACAGAAAACGCAAATAAAAGtt                                                                                                                 >  1:3107465/1‑150 (MQ=255)

                                      tAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAATGtt                                                                                                                 >  1:1452716/1‑150 (MQ=255)

                                      tAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACAACGCATATCAAGGtt                                                                                                                 >  1:2363263/1‑150 (MQ=255)

                                       aaTGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATAAAGGTTa                                                                                                                >  1:673867/1‑150 (MQ=255)

                                         tGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAAc                                                                                                              <  2:233669/150‑1 (MQ=255)

                                         tGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAAc                                                                                                              <  1:705190/150‑1 (MQ=255)

                                           gACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACtg                                                                                                            <  1:4191520/150‑1 (MQ=255)

                                            aCGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACtgt                                                                                                           <  2:4220684/150‑1 (MQ=255)

                                            aCGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACtgt                                                                                                           <  2:902443/150‑1 (MQ=255)

                                               ttttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGcc                                                                                                        <  1:4327281/150‑1 (MQ=255)

                                                tttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCa                                                                                                       <  2:582895/150‑1 (MQ=255)

                                                tttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCa                                                                                                       <  2:3778307/150‑1 (MQ=255)

                                                 ttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCaa                                                                                                      >  1:3780797/1‑150 (MQ=255)

                                                 ttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCaa                                                                                                      >  1:1499574/1‑150 (MQ=255)

                                                   aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAAcc                                                                                                    <  1:2011897/150‑1 (MQ=255)

                                                    gCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATACTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCa                                                                                                   <  2:4042818/150‑1 (MQ=255)

                                                    gCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCa                                                                                                   <  2:2323692/150‑1 (MQ=255)

                                                     ccATAAACGTGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCACGGTAATCTCGAAATTTAGCCAAAGAATACGCAAATCAAGCTTAAATGAGCCAACCAg                                                                                                  >  1:3209938/1‑150 (MQ=255)

                                                     ccATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAg                                                                                                  <  1:3641113/150‑1 (MQ=255)

                                                     ccATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAg                                                                                                  <  1:2468506/150‑1 (MQ=255)

                                                        tAATCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAATCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACt                                                                                               <  2:2388201/150‑1 (MQ=255)

                                                            cGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGt                                                                                           <  1:266400/150‑1 (MQ=255)

                                                            cGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGt                                                                                           <  2:1907714/150‑1 (MQ=255)

                                                             gggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTATCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                          >  2:4206512/1‑150 (MQ=255)

                                                             gggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                          >  1:4105886/1‑150 (MQ=255)

                                                             gggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                          <  1:3667563/150‑1 (MQ=255)

                                                                  tCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCa                                                                                     >  2:3036502/1‑150 (MQ=255)

                                                                   cGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCAt                                                                                    <  1:2134647/150‑1 (MQ=255)

                                                                        aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTATCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGAcc                                                                               >  1:1118015/1‑150 (MQ=255)

                                                                         gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCa                                                                              <  1:1178184/150‑1 (MQ=255)

                                                                          aCGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAAATTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAg                                                                             >  2:2904201/1‑150 (MQ=255)

                                                                           cGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGc                                                                            <  1:429207/150‑1 (MQ=255)

                                                                              gAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACg                                                                         <  1:1770602/150‑1 (MQ=255)

                                                                               aaCGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc                                                                        >  2:2141274/1‑150 (MQ=255)

                                                                               aaCGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc                                                                        >  2:2141792/1‑150 (MQ=255)

                                                                                 cgcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGctc                                                                      <  1:548257/150‑1 (MQ=255)

                                                                                 cgcgAAATAGAATCGTCCTGAACGGCGGCGAGGTCAGCACCAACACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGctc                                                                      <  2:3150491/150‑1 (MQ=255)

                                                                                       atatAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCCGGGTAATCTCGAAATTTAGCCCCAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCCTCGACCAGCACGCTCGTGGTa                                                                >  2:3516573/1‑150 (MQ=255)

                                                                                         ataATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg                                                              <  1:3319795/150‑1 (MQ=255)

                                                                                           aaTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCa                                                            <  1:3930490/150‑1 (MQ=255)

                                                                                           aaTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCa                                                            <  2:506226/150‑1 (MQ=255)

                                                                                            aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAg                                                           <  2:2241/150‑1 (MQ=255)

                                                                                              cGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTa                                                         <  1:3974960/150‑1 (MQ=255)

                                                                                                tCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAc                                                       <  2:2940917/150‑1 (MQ=255)

                                                                                                 ccTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAcc                                                      <  1:868023/150‑1 (MQ=255)

                                                                                                  cTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAccc                                                     <  2:2627671/150‑1 (MQ=255)

                                                                                                   tGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCg                                                    >  2:3290209/1‑150 (MQ=255)

                                                                                                      aCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                                 <  2:2593375/150‑1 (MQ=255)

                                                                                                          cggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaa                                             <  1:1597682/150‑1 (MQ=255)

                                                                                                           ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGTAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaaa                                            >  2:4093892/1‑150 (MQ=255)

                                                                                                           ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaaa                                            >  2:1574938/1‑150 (MQ=255)

                                                                                                             cgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACt                                          <  1:110500/150‑1 (MQ=255)

                                                                                                              gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                         <  2:720121/150‑1 (MQ=255)

                                                                                                                ggTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGAt                                       >  2:1502161/1‑150 (MQ=255)

                                                                                                                      cACCAAAACCTTTATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAttt                                 <  1:2702131/150‑1 (MQ=255)

                                                                                                                        ccAAAACCTTTATCGTCGATTGCCCGGGTTATCTCGAAATTTATCAACAGACTACGCATATAAAGGTTAACTTTTCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCGTCAAACTGATAAATTTTa                               >  2:2805668/1‑150 (MQ=255)

                                                                                                                        ccAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                               <  1:4210308/150‑1 (MQ=255)

                                                                                                                           aaaCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAcc                            <  2:1979142/150‑1 (MQ=255)

                                                                                                                              ccTTTAACGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCcca                         <  2:1399529/150‑1 (MQ=255)

                                                                                                                                 ttATCGTCGATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagca                      <  2:1261359/150‑1 (MQ=255)

                                                                                                                                   aTCGTCGATTGCCAGGGTAATCTCGAAATTTATCCACAGACTAAGCAAATAAAGGTTAACTGTTCCAACCAGACATAGTTCGCCATCGACCAGCACCCTCTTGGTATGCAGTAACCCGCCATAAAACTGATAAATTTTAAACccagcagc                    >  2:2674371/1‑150 (MQ=255)

                                                                                                                                    tcgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc                   <  2:3609398/150‑1 (MQ=255)

                                                                                                                                    tcgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc                   <  2:3609326/150‑1 (MQ=255)

                                                                                                                                     cgtcgATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcca                  <  1:1348679/150‑1 (MQ=255)

                                                                                                                                     cgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcca                  <  2:2363263/150‑1 (MQ=255)

                                                                                                                                        cgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagca               <  1:3550386/150‑1 (MQ=255)

                                                                                                                                         gATTGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagcag              <  1:806292/150‑1 (MQ=255)

                                                                                                                                         gATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagcag              <  1:2844371/150‑1 (MQ=255)

                                                                                                                                          aTTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGt             <  1:3458191/150‑1 (MQ=255)

                                                                                                                                           ttGCCAGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt            >  2:1205181/1‑150 (MQ=255)

                                                                                                                                           ttGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt            <  1:2238262/150‑1 (MQ=255)

                                                                                                                                               cAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt        >  1:4363863/1‑150 (MQ=255)

                                                                                                                                                aGGGTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa       >  2:3088882/1‑150 (MQ=255)

                                                                                                                                                 gggTTATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaa      <  1:3031706/150‑1 (MQ=255)

                                                                                                                                                    tAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGa   >  2:4350232/1‑150 (MQ=255)

CTGAAGAAGTAAAACAGTCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAA  >  CP009273/1301455‑1301753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`