BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,329,391	G→A	14.2%	S4N (AGT→AAT)	ymiA →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,329,391	0	G	A	14.2%	593.8 / 73.9	205	S4N (AGT→AAT)	ymiA	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (102/73); new base A (14/15); total (117/88)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

TCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGG  >  CP009273/1329242‑1329539

tCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAg                                                                                                                                                      <  1:3205321/150‑1 (MQ=255)

tCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATAGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAg                                                                                                                                                      <  1:936868/150‑1 (MQ=255)

      tttCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATgg                                                                                                                                                >  2:500290/1‑150 (MQ=255)

         cAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGa                                                                                                                                             >  1:1385919/1‑150 (MQ=255)

             tatCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATtat                                                                                                                                         <  1:1106488/150‑1 (MQ=255)

                  aTTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAg                                                                                                                                    >  2:2796144/1‑150 (MQ=255)

                  aTTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTAAACCAATCGTTCAACTTCTTGATGATAAGTGATGGCGATTATATGAg                                                                                                                                    >  1:2777468/1‑150 (MQ=255)

                    ttCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGt                                                                                                                                  >  2:465988/1‑150 (MQ=255)

                    ttCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGt                                                                                                                                  >  1:3743947/1‑150 (MQ=255)

                    ttCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGt                                                                                                                                  <  1:3061401/150‑1 (MQ=255)

                       aGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAg                                                                                                                               >  2:3436238/1‑150 (MQ=255)

                       aGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGATTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATAGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAg                                                                                                                               >  1:4296515/1‑150 (MQ=255)

                        ggTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGc                                                                                                                              <  1:3025471/150‑1 (MQ=255)

                          tCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCaa                                                                                                                            >  2:107900/1‑150 (MQ=255)

                           cAAAAGATTGAATTATTCACTTGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAAt                                                                                                                           >  2:2057149/1‑150 (MQ=255)

                             aaaGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGc                                                                                                                         >  2:6664/1‑150 (MQ=255)

                              aaGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGcc                                                                                                                        >  2:3222171/1‑150 (MQ=255)

                              aaGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGcc                                                                                                                        <  2:2226784/150‑1 (MQ=255)

                              aaGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGcc                                                                                                                        <  2:736356/150‑1 (MQ=255)

                               aGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCt                                                                                                                       >  1:449032/1‑150 (MQ=255)

                                      attattCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGaa                                                                                                                >  1:2604515/1‑150 (MQ=255)

                                        tattCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAAt                                                                                                              <  1:2801678/150‑1 (MQ=255)

                                           tCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAg                                                                                                           >  2:1516627/1‑150 (MQ=255)

                                            cACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAgg                                                                                                          <  1:1313742/150‑1 (MQ=255)

                                            cACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAgg                                                                                                          >  2:4498992/1‑150 (MQ=255)

                                            cACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAgg                                                                                                          >  2:452547/1‑150 (MQ=255)

                                              cTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGaa                                                                                                        >  2:3849080/1‑150 (MQ=255)

                                              cTGGAGACGATTCGTAAATTCGCTGGA‑TTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAc                                                                                                       >  2:491534/1‑150 (MQ=255)

                                                  agaCGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAcccc                                                                                                    <  1:2194418/150‑1 (MQ=255)

                                                   gaCGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccg                                                                                                   >  1:2341327/1‑150 (MQ=255)

                                                   gaCGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccg                                                                                                   <  2:1817350/150‑1 (MQ=255)

                                                     cGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCccgcc                                                                                                 >  2:486376/1‑150 (MQ=255)

                                                           gTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATc                                                                                           <  2:1892636/150‑1 (MQ=255)

                                                                ttCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGaa                                                                                      <  1:716737/150‑1 (MQ=255)

                                                                 tCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAAt                                                                                     >  2:3039709/1‑150 (MQ=255)

                                                                 tCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAAt                                                                                     <  1:3678723/150‑1 (MQ=255)

                                                                  cGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAAtt                                                                                    <  2:2864871/150‑1 (MQ=255)

                                                                  cGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAAtt                                                                                    <  1:359990/150‑1 (MQ=255)

                                                                      ggATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTaaaa                                                                                >  1:3270409/1‑150 (MQ=255)

                                                                       gATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAAc                                                                               <  2:3287079/150‑1 (MQ=255)

                                                                          ttttGACTATAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTa                                                                            <  1:2482750/150‑1 (MQ=255)

                                                                          ttttGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTa                                                                            <  1:365439/150‑1 (MQ=255)

                                                                              gACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGc                                                                        >  2:2196136/1‑150 (MQ=255)

                                                                              gACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGc                                                                        >  2:4144787/1‑150 (MQ=255)

                                                                                cTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCct                                                                      <  1:1213759/150‑1 (MQ=255)

                                                                                     gTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCctggctg                                                                 >  1:2450005/1‑150 (MQ=255)

                                                                                         cTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCgg                                                             <  1:3251203/150‑1 (MQ=255)

                                                                                          tttAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGt                                                            <  2:1268193/150‑1 (MQ=255)

                                                                                            tAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGttt                                                          >  1:1852488/1‑150 (MQ=255)

                                                                                            tAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGttt                                                          <  1:3789584/150‑1 (MQ=255)

                                                                                            tAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGttt                                                          >  1:1852232/1‑150 (MQ=255)

                                                                                             aGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGtttt                                                         >  2:2604859/1‑150 (MQ=255)

                                                                                              ggATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGttttt                                                        <  1:3222171/150‑1 (MQ=255)

                                                                                               gATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTc                                                       <  1:389143/150‑1 (MQ=255)

                                                                                                aTTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCt                                                      >  1:1596006/1‑150 (MQ=255)

                                                                                                aTTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCt                                                      <  1:2649964/150‑1 (MQ=255)

                                                                                                aTTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCt                                                      <  1:453875/150‑1 (MQ=255)

                                                                                                aTTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCt                                                      <  1:2524642/150‑1 (MQ=255)

                                                                                                 tttGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCtt                                                     <  2:4198922/150‑1 (MQ=255)

                                                                                                   tGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTGTTgg                                                   >  1:3111770/1‑150 (MQ=255)

                                                                                                     tctcATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGtt                                                 <  1:2716098/150‑1 (MQ=255)

                                                                                                         aTCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGATGGCGGTTTTTCTTGGTTCTGc                                             >  2:1974334/1‑150 (MQ=255)

                                                                                                              aTGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACttt                                        >  1:1830465/1‑150 (MQ=255)

                                                                                                               tGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACtttt                                       >  1:3686390/1‑150 (MQ=255)

                                                                                                                  ttaattaCACCAATCGTTCCACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTg                                    <  2:2332958/150‑1 (MQ=255)

                                                                                                                  ttaattaCACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTg                                    <  2:1439256/150‑1 (MQ=255)

                                                                                                                  ttaattaCACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTg                                    <  2:2590713/150‑1 (MQ=255)

                                                                                                                  ttaattaCACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCGGGCGGTTTTTCTTGGTTCTGCACTTTTCTg                                    <  2:324873/150‑1 (MQ=255)

                                                                                                                   taattaCACCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGGTCTTGACTTTTCTgg                                   >  1:400872/1‑150 (MQ=255)

                                                                                                                    aattaCACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTggg                                  >  1:1826948/1‑150 (MQ=255)

                                                                                                                    aattaCACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTggg                                  >  1:2370082/1‑150 (MQ=255)

                                                                                                                        acacCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGggtggt                              >  2:2061245/1‑150 (MQ=255)

                                                                                                                         cacCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGtt                             >  1:762500/1‑150 (MQ=255)

                                                                                                                          acCAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTg                            <  2:3395584/150‑1 (MQ=255)

                                                                                                                            cAATCGTTCAACGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTc                          <  2:3423691/150‑1 (MQ=255)

                                                                                                                             aaTCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCg                         >  2:4034383/1‑150 (MQ=255)

                                                                                                                                 gTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACATTTATGGGTGGTTGTCGCAct                     >  1:840972/1‑150 (MQ=255)

                                                                                                                                    cAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCActgct                  <  2:2877227/150‑1 (MQ=255)

                                                                                                                                     aaCGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCActgctg                 >  2:563853/1‑150 (MQ=255)

                                                                                                                                     aaCGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCActgctg                 <  2:3270409/150‑1 (MQ=255)

                                                                                                                                     aaCGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCActgctg                 <  2:1840519/150‑1 (MQ=255)

                                                                                                                                     aaCGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTGTGGTGGTTCTGCACTTTTCTGGGTGGTTGTCGCActgctg                 >  1:2022858/1‑150 (MQ=255)

                                                                                                                                     aaCGTCTTGCTGATAAATGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCActgctg                 >  1:105414/1‑150 (MQ=255)

                                                                                                                                      aCGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGa                <  1:3362308/150‑1 (MQ=255)

                                                                                                                                        gTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGAtt              <  1:466050/150‑1 (MQ=255)

                                                                                                                                         tCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGAttt             <  1:2754121/150‑1 (MQ=255)

                                                                                                                                          cTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTg            >  2:3103969/1‑150 (MQ=255)

                                                                                                                                          cTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTg            <  1:1733959/150‑1 (MQ=255)

                                                                                                                                            tGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGa          >  1:1390119/1‑150 (MQ=255)

                                                                                                                                            tGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGa          >  1:1242992/1‑150 (MQ=255)

                                                                                                                                            tGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGa          >  1:4260975/1‑150 (MQ=255)

                                                                                                                                            tGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTATTTGTTATGCACATTTCTGGGTGGTTTTAGCAATTCTGATTTTGa          >  1:1512762/1‑150 (MQ=255)

                                                                                                                                             gCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGaa         <  1:3316440/150‑1 (MQ=255)

                                                                                                                                              cTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGaaa        >  2:3704925/1‑150 (MQ=255)

                                                                                                                                                gATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAgt      >  1:2863612/1‑150 (MQ=255)

                                                                                                                                                 aTAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTGTTGGTTCTGCACTTTTCTGGGTGGGTGTCGCACAGCGGATTTGGAAAgtg     >  1:404283/1‑150 (MQ=255)

                                                                                                                                                 aTAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAgtg     >  1:2087100/1‑150 (MQ=255)

                                                                                                                                                 aTAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAgtg     <  1:514639/150‑1 (MQ=255)

                                                                                                                                                 aTAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAgtg     <  2:1385919/150‑1 (MQ=255)

                                                                                                                                                    aGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTgg  >  1:3777227/1‑150 (MQ=255)

TCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGG  >  CP009273/1329242‑1329539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`