Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,337,533 G→T 87.6% A18E (GCA→GAA)  osmB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,337,5330GT87.6% 688.6 / 89.9 248A18E (GCA→GAA) osmBlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (16/15);  new base T (103/114);  total (119/129)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

CCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCT  >  CP009273/1337386‑1337678
                                                                                                                                                   |                                                                                                                                                 
ccAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTcc                                                                                                                                                 >  1:4187247/1‑150 (MQ=255)
  aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaa                                                                                                                                               >  2:4175024/1‑150 (MQ=255)
  aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaa                                                                                                                                               >  2:1897560/1‑150 (MQ=255)
  aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaa                                                                                                                                               >  1:84382/1‑150 (MQ=255)
    tAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAg                                                                                                                                             <  2:813391/150‑1 (MQ=255)
     aaCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGt                                                                                                                                            >  2:679651/1‑150 (MQ=255)
     aaCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGt                                                                                                                                            <  2:907063/150‑1 (MQ=255)
      acacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTa                                                                                                                                           <  1:1132796/150‑1 (MQ=255)
      acacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTa                                                                                                                                           >  1:299627/1‑150 (MQ=255)
       cacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTaa                                                                                                                                          <  1:3040568/150‑1 (MQ=255)
        acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAt                                                                                                                                         >  2:2767016/1‑150 (MQ=255)
           tCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGc                                                                                                                                      >  1:1662258/1‑150 (MQ=255)
            ccGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGcc                                                                                                                                     <  1:3345904/150‑1 (MQ=255)
             cGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCa                                                                                                                                    >  1:1287840/1‑150 (MQ=255)
                  gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                               <  2:79633/150‑1 (MQ=255)
                    tGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGc                                                                                                                             >  2:3710529/1‑150 (MQ=255)
                     gCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGcc                                                                                                                            <  1:2593840/150‑1 (MQ=255)
                      caccacCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCcg                                                                                                                           <  2:1348275/150‑1 (MQ=255)
                            cTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCa                                                                                                                     <  1:574834/150‑1 (MQ=255)
                                   aCCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttttt                                                                                                              <  1:1013500/150‑1 (MQ=255)
                                      cAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCTCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCt                                                                                                           >  1:3757116/1‑150 (MQ=255)
                                          gTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGtt                                                                                                       <  2:1441054/150‑1 (MQ=255)
                                            aCTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAc                                                                                                     >  2:2988354/1‑150 (MQ=255)
                                            aCTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAc                                                                                                     >  2:725293/1‑150 (MQ=255)
                                             cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTACTTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACa                                                                                                    >  2:3087806/1‑150 (MQ=255)
                                                       tCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAc                                                                                          <  2:639044/150‑1 (MQ=255)
                                                        cAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCCGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAct                                                                                         >  2:2916521/1‑150 (MQ=255)
                                                        cAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAct                                                                                         >  1:2258/1‑150 (MQ=255)
                                                         aGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActc                                                                                        >  2:3611811/1‑150 (MQ=255)
                                                         aGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActc                                                                                        >  1:2370012/1‑150 (MQ=255)
                                                          gTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActct                                                                                       <  2:1301448/150‑1 (MQ=255)
                                                            aCTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctct                                                                                     <  1:333035/150‑1 (MQ=255)
                                                              tGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTcc                                                                                   <  2:4461897/150‑1 (MQ=255)
                                                               gCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCt                                                                                  <  1:4443001/150‑1 (MQ=255)
                                                                cACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACCGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTg                                                                                 >  1:1580824/1‑150 (MQ=255)
                                                                 aCCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGa                                                                                >  2:4338040/1‑150 (MQ=255)
                                                                      cTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTa                                                                           >  1:1959691/1‑150 (MQ=255)
                                                                       tAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAt                                                                          <  2:1663903/150‑1 (MQ=255)
                                                                       tAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAt                                                                          >  1:1044073/1‑150 (MQ=255)
                                                                        aaTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACCGCCACTCAAAGACATTGCCAAAGGAAATGCCAGACCAGCCGCGGTCATAATTTGGCTCGTTACAAAAATAATACTCTCTCCTGACTTTATg                                                                         >  1:2756685/1‑150 (MQ=255)
                                                                        aaTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATg                                                                         >  2:1078439/1‑150 (MQ=255)
                                                                        aaTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATg                                                                         <  1:2847708/150‑1 (MQ=255)
                                                                            ccccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTc                                                                     <  1:3509793/150‑1 (MQ=255)
                                                                            ccccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTc                                                                     <  1:1446210/150‑1 (MQ=255)
                                                                            ccccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTc                                                                     <  1:2439122/150‑1 (MQ=255)
                                                                             cccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCa                                                                    >  2:1774618/1‑150 (MQ=255)
                                                                             cccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCa                                                                    >  2:2188591/1‑150 (MQ=255)
                                                                             cccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCa                                                                    <  2:765498/150‑1 (MQ=255)
                                                                              ccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCAc                                                                   >  2:199374/1‑150 (MQ=255)
                                                                                 gCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGa                                                                >  1:1829479/1‑150 (MQ=255)
                                                                                  cACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGaa                                                               <  1:4503256/150‑1 (MQ=255)
                                                                                   aCCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAg                                                              <  1:4525672/150‑1 (MQ=255)
                                                                                     ccGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTa                                                            <  2:2215464/150‑1 (MQ=255)
                                                                                         gcCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGct                                                        >  2:527744/1‑150 (MQ=255)
                                                                                          ccGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctc                                                       >  2:3565778/1‑150 (MQ=255)
                                                                                           cGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                      >  1:605755/1‑150 (MQ=255)
                                                                                           cGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                      >  1:463143/1‑150 (MQ=255)
                                                                                            gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                     >  1:3009117/1‑150 (MQ=255)
                                                                                            gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                     <  2:1853945/150‑1 (MQ=255)
                                                                                            gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                     <  1:4195769/150‑1 (MQ=255)
                                                                                             aTTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctct                                                    <  1:3983661/150‑1 (MQ=255)
                                                                                               tGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctat                                                  >  2:252922/1‑150 (MQ=255)
                                                                                               tGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctat                                                  >  1:707667/1‑150 (MQ=255)
                                                                                                 cGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataa                                                <  2:1879771/150‑1 (MQ=255)
                                                                                                 cGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataa                                                <  1:1211391/150‑1 (MQ=255)
                                                                                                 cGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataa                                                <  1:3499425/150‑1 (MQ=255)
                                                                                                     gtTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataactat                                            <  1:3231059/150‑1 (MQ=255)
                                                                                                        gCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataat                                         >  1:3125671/1‑150 (MQ=255)
                                                                                                          ggTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAg                                       <  1:4349316/150‑1 (MQ=255)
                                                                                                          ggTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAg                                       >  1:3864183/1‑150 (MQ=255)
                                                                                                           gTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGc                                      <  2:1183788/150‑1 (MQ=255)
                                                                                                             cccGTTTAGAACAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTa                                    <  2:4372306/150‑1 (MQ=255)
                                                                                                               cGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAg                                  >  1:1916685/1‑150 (MQ=255)
                                                                                                               cGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAg                                  <  2:1673487/150‑1 (MQ=255)
                                                                                                               cGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAg                                  <  1:713932/150‑1 (MQ=255)
                                                                                                                gTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGa                                 <  2:242396/150‑1 (MQ=255)
                                                                                                                gTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGa                                 <  1:4324830/150‑1 (MQ=255)
                                                                                                                gTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGa                                 <  1:3738484/150‑1 (MQ=255)
                                                                                                                    aGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATaa                             >  2:1252749/1‑150 (MQ=255)
                                                                                                                    aGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATaa                             <  1:3055648/150‑1 (MQ=255)
                                                                                                                     gACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAg                            <  2:2368687/150‑1 (MQ=255)
                                                                                                                       ccAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTc                          >  1:3442247/1‑150 (MQ=255)
                                                                                                                         aGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTg                        >  1:3145114/1‑150 (MQ=255)
                                                                                                                           ttAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGt                      <  1:3856505/150‑1 (MQ=255)
                                                                                                                            tAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAATCTCTCTATAAATATAATATCTAAGAATAAGTCTGTTg                     >  2:2696743/1‑150 (MQ=255)
                                                                                                                            tAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTg                     >  1:2310449/1‑150 (MQ=255)
                                                                                                                                 cAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatt                <  1:2094530/150‑1 (MQ=255)
                                                                                                                                  aGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta               <  2:3428398/150‑1 (MQ=255)
                                                                                                                                  aGGCACTCAGAGACATGTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTGATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta               <  2:1141555/150‑1 (MQ=255)
                                                                                                                                   ggCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAattat              <  2:4064501/150‑1 (MQ=255)
                                                                                                                                     cACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTc            <  1:722112/150‑1 (MQ=255)
                                                                                                                                     cACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTc            <  1:1360246/150‑1 (MQ=255)
                                                                                                                                     cACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTc            >  1:3920110/1‑150 (MQ=255)
                                                                                                                                     cACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTc            <  2:247752/150‑1 (MQ=255)
                                                                                                                                      aCTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTcc           >  1:629416/1‑150 (MQ=255)
                                                                                                                                         cAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg        >  2:3140911/1‑150 (MQ=255)
                                                                                                                                         cAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg        >  1:1727560/1‑150 (MQ=255)
                                                                                                                                         cAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTACGTg        >  2:4471341/1‑150 (MQ=255)
                                                                                                                                          agagACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGa       <  1:2191758/150‑1 (MQ=255)
                                                                                                                                              aCATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATc   >  2:262481/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCT  >  CP009273/1337386‑1337678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑