BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,610,407	C→T	10.0%	P130S (CCC→TCC)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,610,407	0	C	T	10.0%	614.6 / 42.4	190	P130S (CCC→TCC)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (90/81); new base T (11/8); total (101/89)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCC  >  CP009273/1610258‑1610556

tataCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCCGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATGTTTTTCGCTc                                                                                                                                                       >  2:3545450/1‑150 (MQ=255)

 ataCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcc                                                                                                                                                      <  2:2783250/150‑1 (MQ=255)

 ataCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcc                                                                                                                                                      <  2:398838/150‑1 (MQ=255)

  taCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTtcc                                                                                                                                                     <  2:3377224/150‑1 (MQ=255)

  taCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTccc                                                                                                                                                     >  2:3363065/1‑150 (MQ=255)

    cAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcccaa                                                                                                                                                   >  1:986285/1‑150 (MQ=255)

      ttGAAAAGGTTCAGTTTAAACTCACTTATCCAGCCAGGAATGTAAGACACAAAGAAAATATTTAATTTCAGGTTGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTtccaacc                                                                                                                                                 <  2:3423685/149‑1 (MQ=255)

      aTGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTtccaacc                                                                                                                                                 <  2:1137681/150‑1 (MQ=255)

       tGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcccaaccc                                                                                                                                                >  1:3582648/1‑150 (MQ=255)

        gAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTtccaaccca                                                                                                                                               <  1:146031/150‑1 (MQ=255)

           aaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAg                                                                                                                                            >  2:2695946/1‑150 (MQ=255)

            aGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTTCCAACCCAAAGc                                                                                                                                           >  2:3030067/1‑150 (MQ=255)

              gTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCt                                                                                                                                         <  1:1487305/150‑1 (MQ=255)

                tCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGt                                                                                                                                       <  2:4254753/150‑1 (MQ=255)

                 cAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTc                                                                                                                                      <  2:1898255/150‑1 (MQ=255)

                    tttAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACt                                                                                                                                   <  2:746081/150‑1 (MQ=255)

                      tAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATATTTTGCGCTCCCAACCCAAAGCCTGTCACtct                                                                                                                                 >  2:3633133/1‑148 (MQ=255)

                        aaCTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATgg                                                                                                                               >  1:4534434/1‑150 (MQ=255)

                          cTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATgggg                                                                                                                             <  2:2908647/150‑1 (MQ=255)

                          cTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATgggg                                                                                                                             <  1:3709873/150‑1 (MQ=255)

                           tCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGgc                                                                                                                            >  2:456155/1‑150 (MQ=255)

                               ttATCCCGCCAGTAATGGAAGACACAAAGAAAATTTTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGt                                                                                                                        <  2:584096/150‑1 (MQ=255)

                               ttATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTTCCAACCCAAAGCCTGTCACTATGGGGCGCGt                                                                                                                        >  2:1177727/1‑150 (MQ=255)

                                 aTCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTCTGGGGCGCGTAt                                                                                                                      >  1:3913293/1‑150 (MQ=255)

                                    ccGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTCTGGGGCGCGTATTTc                                                                                                                   >  2:1805114/1‑150 (MQ=255)

                                     cGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCa                                                                                                                  >  2:3873648/1‑150 (MQ=255)

                                      gCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAg                                                                                                                 >  1:2656889/1‑150 (MQ=255)

                                       ccAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGa                                                                                                                <  2:4523321/150‑1 (MQ=255)

                                       ccAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGa                                                                                                                <  2:4523167/150‑1 (MQ=255)

                                          ggAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCg                                                                                                             <  2:2080766/150‑1 (MQ=255)

                                                   gACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTTCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGc                                                                                                    >  1:563435/1‑150 (MQ=255)

                                                    acacAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCg                                                                                                   <  2:3447126/150‑1 (MQ=255)

                                                    acacAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCg                                                                                                   <  2:4208046/150‑1 (MQ=255)

                                                     cacaAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCgt                                                                                                  <  1:3438087/150‑1 (MQ=255)

                                                        aaaGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTa                                                                                               >  1:482918/1‑150 (MQ=255)

                                                          aGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTAct                                                                                             >  2:2929576/1‑150 (MQ=255)

                                                          aGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTAct                                                                                             >  1:168943/1‑150 (MQ=255)

                                                          aGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTAct                                                                                             >  2:3754428/1‑150 (MQ=255)

                                                            aaaaTATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTActct                                                                                           >  1:2102604/1‑150 (MQ=255)

                                                             aaaTATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTTCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtt                                                                                          <  2:3800975/150‑1 (MQ=255)

                                                             aaaTATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtt                                                                                          <  1:3952235/150‑1 (MQ=255)

                                                                  ttGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtttctgt                                                                                     <  2:271765/150‑1 (MQ=255)

                                                                   tGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtttctgtt                                                                                    <  1:4251035/150‑1 (MQ=255)

                                                                   tGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtttctgtt                                                                                    >  1:2817042/1‑150 (MQ=255)

                                                                     aaTTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtttctgtttc                                                                                  <  1:1512848/150‑1 (MQ=255)

                                                                     aaTTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGACTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCtttctgtttc                                                                                  >  2:4384636/1‑150 (MQ=255)

                                                                          cAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAAt                                                                             <  2:650338/150‑1 (MQ=255)

                                                                            ggTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGa                                                                           <  2:3480039/150‑1 (MQ=255)

                                                                            ggTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGa                                                                           >  1:2938021/1‑150 (MQ=255)

                                                                             gtagtaATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGAt                                                                          <  1:2672154/150‑1 (MQ=255)

                                                                             gtagtaATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGAt                                                                          <  1:3294793/150‑1 (MQ=255)

                                                                               agtaATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGAttt                                                                        >  1:1125682/1‑150 (MQ=255)

                                                                                  aataaATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTAt                                                                     >  2:3261976/1‑150 (MQ=255)

                                                                                  aataaATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTAt                                                                     <  1:3084085/150‑1 (MQ=255)

                                                                                       aTTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAAc                                                                >  1:2949261/1‑150 (MQ=255)

                                                                                          tAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAAt                                                             >  1:900634/1‑150 (MQ=255)

                                                                                           aaGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAAtt                                                            <  1:3338595/150‑1 (MQ=255)

                                                                                             gCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTc                                                          >  2:2403381/1‑150 (MQ=255)

                                                                                             gCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTc                                                          <  2:1683786/150‑1 (MQ=255)

                                                                                             gCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTc                                                          <  2:2838729/150‑1 (MQ=255)

                                                                                                 tatttattTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAAt                                                      <  2:2203571/150‑1 (MQ=255)

                                                                                                 tatttattTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAAt                                                      <  2:2204284/150‑1 (MQ=255)

                                                                                                 tatttattTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAAt                                                      <  1:1847986/150‑1 (MQ=255)

                                                                                                    ttatttaGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGAt                                                   <  1:2893952/150‑1 (MQ=255)

                                                                                                    ttatttaGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGAt                                                   <  1:2468027/150‑1 (MQ=255)

                                                                                                    ttatttaGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGAt                                                   <  1:3703280/150‑1 (MQ=255)

                                                                                                     tatttaGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATc                                                  <  1:4413484/150‑1 (MQ=255)

                                                                                                      atttaGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCa                                                 <  1:2544636/150‑1 (MQ=255)

                                                                                                         taGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAAcc                                              >  1:475076/1‑150 (MQ=255)

                                                                                                            aTAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTTCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTGCTGTTTATGAATGATTTTATTGAACAATTTCAAATGATCAACCCTg                                           >  1:41222/1‑150 (MQ=255)

                                                                                                              aaTTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGgt                                         <  1:1807064/150‑1 (MQ=255)

                                                                                                              aaTTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGgt                                         <  1:3750376/150‑1 (MQ=255)

                                                                                                                  ttCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTc                                     <  1:4513427/150‑1 (MQ=255)

                                                                                                                    cGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTccc                                   >  2:3162287/1‑150 (MQ=255)

                                                                                                                     gCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCa                                  <  2:3582648/150‑1 (MQ=255)

                                                                                                                     gCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCa                                  <  1:3168310/150‑1 (MQ=255)

                                                                                                                      ccATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCAt                                 <  1:543230/150‑1 (MQ=255)

                                                                                                                       cATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATc                                >  1:1273160/1‑150 (MQ=255)

                                                                                                                       cATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATc                                >  2:2414350/1‑150 (MQ=255)

                                                                                                                        atgatgGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCa                               <  1:1960633/150‑1 (MQ=255)

                                                                                                                            tgGAGAAATTAATATTTTTTGCGCTTCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAag                           >  2:1086197/1‑150 (MQ=255)

                                                                                                                             ggAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAaga                          >  2:1240689/1‑150 (MQ=255)

                                                                                                                             ggAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAaga                          >  2:3821500/1‑150 (MQ=255)

                                                                                                                             ggAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAaga                          >  2:1540773/1‑150 (MQ=255)

                                                                                                                                gaAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAgcg                       >  2:2392878/1‑150 (MQ=255)

                                                                                                                                gaAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAgcg                       >  2:1954865/1‑150 (MQ=255)

                                                                                                                                 aaaTTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAgcgc                      <  1:2710575/150‑1 (MQ=255)

                                                                                                                                     tAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATac                  >  2:66075/1‑150 (MQ=255)

                                                                                                                                     tAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATac                  >  1:2444384/1‑150 (MQ=255)

                                                                                                                                      aaTATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATaca                 >  1:22613/1‑150 (MQ=255)

                                                                                                                                       atatTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATacac                >  2:4157795/1‑150 (MQ=255)

                                                                                                                                         atTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATAcacca              <  1:2058000/150‑1 (MQ=255)

                                                                                                                                           tttttGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACcaca            <  1:3010193/150‑1 (MQ=255)

                                                                                                                                              ttGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACatat         <  1:1056160/150‑1 (MQ=255)

                                                                                                                                                 cgcTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGa      >  1:1636937/1‑150 (MQ=255)

                                                                                                                                                  gcTTCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGaa     >  2:4323112/1‑150 (MQ=255)

                                                                                                                                                   cTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAAc    <  2:2485920/150‑1 (MQ=255)

                                                                                                                                                     cccaacccaaAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTTCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAAccc  <  2:1457189/150‑1 (MQ=255)

                                                                                                                                                     cccaacccaaAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAAccc  <  2:3949077/150‑1 (MQ=255)

                                                                                                                                                     cccaacccaaAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAAccc  >  2:3667997/1‑150 (MQ=255)

TATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGCGCGTATTTCAGACCGGCGTCGAGCGTGTACTCTTTCTGTTTCTGAATGATTTTATTGAACAATTTCCAATGATCAACCCTGGTGTTCCCATCAAAAGAGCGCATACACCACATATTGAACCC  >  CP009273/1610258‑1610556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`