Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,673,421 T→A 11.0% T246T (ACT→ACA)  ydgH → DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,673,4210TA11.0% 513.8 / 7.2 201T246T (ACT→ACA) ydgHDUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (94/72);  new base A (18/10);  total (119/82)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

GCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCA  >  CP009273/1673272‑1673569
                                                                                                                                                     |                                                                                                                                                    
gCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACt                                                                                                                                                      <  1:2011832/150‑1 (MQ=255)
 cAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTa                                                                                                                                                     >  2:1399573/1‑150 (MQ=255)
     tctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCCGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                                                                 >  2:2397469/1‑150 (MQ=255)
      ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTc                                                                                                                                                >  1:1614526/1‑150 (MQ=255)
      ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTc                                                                                                                                                <  1:2980672/150‑1 (MQ=255)
             ccGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACAAAAGTCGAAGAAc                                                                                                                                         >  1:208365/1‑150 (MQ=255)
             ccGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCATTGAAACCCACTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACGAATGTCGAAGAAc                                                                                                                                         >  2:1897915/1‑150 (MQ=255)
               ggTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTg                                                                                                                                       <  2:1873128/150‑1 (MQ=255)
                 tGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCGTCTTTGAAAACCAGTCCTCAAAAGGCGGGCGGTACACCGTCACCCTCCCCGAAGGCACAAAAGTCGCAGAACTGaa                                                                                                                                     >  1:1566260/1‑150 (MQ=255)
                  ggagAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGGTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAAc                                                                                                                                    >  2:3015523/4‑150 (MQ=255)
                    cGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTGTTTTAAAACCAGTCATAAAAATGCGGGCGTTTAAACGTCACGCAACCCGATGGCACAAAATTCGAAGAAATTAACaa                                                                                                                                  >  1:2355351/1‑150 (MQ=255)
                    cGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACAGAACaa                                                                                                                                  >  1:1760460/1‑150 (MQ=255)
                     gAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACAAAAGTCGAAGAACTGAACaaa                                                                                                                                 >  1:3608085/1‑150 (MQ=255)
                       aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGc                                                                                                                               <  2:215921/150‑1 (MQ=255)
                       aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGc                                                                                                                               <  2:1684497/150‑1 (MQ=255)
                       aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCCTCACGCTCCCGGATGGCACAAAATTCGAAGAACTGAACAAAGc                                                                                                                               >  1:4405320/1‑150 (MQ=255)
                        gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                                                              <  1:4215684/150‑1 (MQ=255)
                        gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                                                              <  1:1935454/150‑1 (MQ=255)
                        gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                                                              <  1:3765028/150‑1 (MQ=255)
                        gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                                                              <  1:3763871/150‑1 (MQ=255)
                        gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                                                              <  1:369254/150‑1 (MQ=255)
                        gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                                                              <  2:1551809/150‑1 (MQ=255)
                         ccGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGa                                                                                                                             >  1:1050933/1‑150 (MQ=255)
                         ccGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGa                                                                                                                             <  1:1161672/150‑1 (MQ=255)
                          cgcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTATTTTAAACCCACTCAACCAAAGGCGGGCGGTAAACCCTCACCCACCCGGATGGCACAAAAGTCGAAGAACAGAACaaaaccac                                                                                                                            >  1:3207973/1‑145 (MQ=255)
                                gaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTAATCAAAAGGCGGGGGTTTCACAGGCACGCTCCCGGATGGCACAAAAGTCGAAGAACAGAACAAAGCGACCCCAGc                                                                                                                      >  1:502488/1‑150 (MQ=255)
                                   tgtttataTCCGGGTTGTTGCTAATACCGAATCACCATGTTCTGATGTCGGTCGCTTATTTTAAACCCAGTCATAAAATGGGGAGGGTTACACCTTCACGCTCCGTTTTGGCAATAAAGTCTAATAACTTAACAAAGCGACCGCAGCgat                                                                                                                   <  2:474046/143‑1 (MQ=255)
                                        ttaTCCGGGGTTTTGCGACTACCGAATCACCATTTTCTGATGGCGGGCTCTTGTTTTAAACCCTGTAATAAAATGGGGGGCTTTACACCTTCCCGCGCCGGTTTGGAAATAACGTCGAAGAACTTAAAAAAGCGACCGCAGCGATGATgg                                                                                                              <  2:1124398/148‑1 (MQ=255)
                                         gaTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCATCAAAAGGCGGGCGTTAAACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAACCGACCGCAGCGATGATGGt                                                                                                             >  1:3558884/1‑150 (MQ=255)
                                         gaTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTATTTGTAAACCAGTCATAAAAAGGCGGGCGTTTCACCGGCACGCTCCCGGCTGGCCCTAAAGTGGAAGAAATGAACAAATCGACCGCCGCGCTGATGGt                                                                                                             >  1:51616/1‑150 (MQ=255)
                                          agcccGGGGGTTGCGACTACCGAATCACAATGTTCTGACGTCGGTCGATTCTTTCAAACCCCGAAAACAAACGGCGGGCGTTACACCGCCACGCGCCCGTTTGGCACTACAGTCGAAGAACTGAACACAGCGACCGCAGCGATGATGGTc                                                                                                            <  2:3060027/148‑1 (MQ=255)
                                           tCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAATTCGAATAAATGAACAAAGCGACCGCAGCGATGATGGTcc                                                                                                           >  1:1705954/1‑150 (MQ=255)
                                           tCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAATCGACCGCAGCGCTGATGGTcc                                                                                                           >  1:3984724/1‑150 (MQ=255)
                                              cGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAAACCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACGAAAGTCGAAGAACTGAACAAAGCGACCCCACCGAAGATGGTcccct                                                                                                        >  2:2684147/1‑148 (MQ=255)
                                              cGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAATGCGGGCGTTACACCGTCACGCTCCCGGATGGCACGAAAGTCGAAGAACTGAAAAAAGCGACCGCAGCGATGATGGTCCCGt                                                                                                        >  2:416046/1‑150 (MQ=255)
                                               gggTGTTGCTCCAACCCCATCACCATGTTCTTATGTCGGTCGCTTTTTTAAAACCCCCAAAACAACAGGGGGGGGTACCACCGACACGCTCCGGGTGGGAACTAACGTCCAATAACTGAAACACGCGACCGCAGCGATGATGGTCCCGtt                                                                                                       <  2:3789837/150‑1 (MQ=255)
                                               gggTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCATAAAAAGGCGGGCGGTACACCGTCACCCGCCCGGATGGCACGAAAGTCGAAGAACTGTACAAAGCGACCGCAGCGATGATGGTCCCGtt                                                                                                       >  1:2812743/1‑150 (MQ=255)
                                                ggTGTTGCTACTACCGAATCACCATTTTCGGAAGTCGGTCGATTCTTTTAAACCCAGTCATCAAAAGGGGGGCTTTACACCGTCACGCGCCCGTATGGCACAAACGTCTAACAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTc                                                                                                      <  2:3835337/150‑1 (MQ=255)
                                                 tggtTGCGACAACCGCAACACCATGTTCTGATGGCGGTCGCTTCTTTGAAACCCCGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGTTGGCACTACAGCCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCg                                                                                                     <  2:3441753/148‑1 (MQ=255)
                                                     tGCTACTACCGAATCACAATGTTCGGAAGTCGGTCGCTTCTTTCAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTTTGGCAATAAAGTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAg                                                                                                 <  2:276198/150‑1 (MQ=255)
                                                     tGCGACTACCGCATCACCATGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCAGAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGAACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAg                                                                                                 <  2:1824175/150‑1 (MQ=255)
                                                       cGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTGTTTTAAAACCACTACTCAAAAGGCGGGCGTTACACCGTCACGCGCCCCGATGGCACGAAAGTAGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCCGTCGAAAGcc                                                                                               >  1:2340947/1‑149 (MQ=255)
                                                       cGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGTAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCACCCCGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCa                                                                                               >  1:987487/1‑150 (MQ=255)
                                                        gACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCAt                                                                                              >  2:3771244/1‑150 (MQ=255)
                                                           tACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACAACGTCACGCTCCCGGATGGCACTAAAGTCGCAGAACTGAACAAAGCGACCGCCGCGATGATGGTCCCGGTCGCCAGCATCaa                                                                                           >  2:2279158/1‑150 (MQ=255)
                                                            aCCGCATCACCAAGTTCTGAAGTCGGTCGCGTCGTTTAAACCCAGTAATCAAAAGGCGGGCGTTACAACGTCACGCTCCCCGATGGCACAAAAGTCGAAGAACAGAAAAAAGCCACCGCAGCGATTATGGTCCCGGTCGACAGCATCaaa                                                                                          >  1:3235623/1‑150 (MQ=255)
                                                                 aTCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCTGTCATCAAAAGGGGGGCGTTACACCGTCACGCTCCCGGTTGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                                                                     <  2:4126512/150‑1 (MQ=255)
                                                                     ccAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAATCGACCGCAGCGATGATTGTCCCGTTCGACAGCATCAAATTCTCTTGc                                                                                 >  2:1969946/1‑150 (MQ=255)
                                                                      catgTTCTGACGTCGGTCGATTCTTTGAAACCCAGTAATAAAAAGGCGGGGGTTCCACCGTCACGCTCCCGGTTGGCACTAACGTCGAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCa                                                                                <  2:4197481/147‑1 (MQ=255)
                                                                       aaGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCaa                                                                               >  2:3277881/1‑150 (MQ=255)
                                                                         gTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACt                                                                             >  2:2980606/1‑150 (MQ=255)
                                                                                 gtgggtcgCTTCTTTTAAACCCCGCCATAAATTGGCGGGCTTAACACCGTCCCGCTCCCGGTTGGCACTAAAGCCCAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAAc                                                                     <  2:1576986/147‑1 (MQ=255)
                                                                                 gtcggtcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAATAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAAc                                                                     <  2:3396702/150‑1 (MQ=255)
                                                                                   gggtcgCTTCTTTGAAACCCAGTCAACAAATGGCGGGGGTTACACCGTCACGCTCCCGGTTGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACAt                                                                   <  2:2377131/149‑1 (MQ=255)
                                                                                   cggtcgCTTCTTTGAAACCCAGACATCAAAAGGCCGGCGTTACACCGGCACGCTCCCCGCAGGCACAAAAGTCGAAGAACAGAACAAAACGACCGCAGCGAATATGGTCCCGTTCGACAGCAACAAAATATCAGGCAACAACCGCAACaa                                                                   >  2:408975/1‑149 (MQ=255)
                                                                                     gtcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                                                                 >  2:414819/1‑150 (MQ=255)
                                                                                     gtcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                                                                 >  2:2861968/1‑150 (MQ=255)
                                                                                      tcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCTTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGACCAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                                                                <  2:3637999/150‑1 (MQ=255)
                                                                                      tcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                                                                >  1:656869/1‑150 (MQ=255)
                                                                                      tcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                                                                <  2:316318/150‑1 (MQ=255)
                                                                                        gCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCg                                                              >  2:591169/1‑150 (MQ=255)
                                                                                         cttcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGa                                                             >  2:1614574/1‑150 (MQ=255)
                                                                                         cttcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGa                                                             >  1:3705988/1‑150 (MQ=255)
                                                                                            cttTGAACCCCAGTCATCAAACGGCGGGCGTTACCCCGTCCCGCGCCCGGATGCCACAAAAGACGAATAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGt                                                          <  1:1401052/150‑1 (MQ=255)
                                                                                            atttGAAACCCAGTCATAAAATGGCGGGCTTTACACCGTCACGCTCCCGTATGGCACTAAAGTCTAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGt                                                          <  2:2669760/149‑1 (MQ=255)
                                                                                               tGAAACCCAGTCATCAAAAGGGGGGCGTTACACCGTCACGCTCCCGGTTGGCAATAAAGTCTAACAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc                                                       <  2:1928899/150‑1 (MQ=255)
                                                                                                taaaCCCAGTCATCAACCGGGGGGCGTTCCACCGACACGCGCCCGGATGGCACTAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTcc                                                      <  1:667324/149‑1 (MQ=255)
                                                                                                gAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTcc                                                      >  1:1336581/1‑150 (MQ=255)
                                                                                                 aaaCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCt                                                     >  1:2649526/1‑150 (MQ=255)
                                                                                                 aaaCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCt                                                     >  1:382007/1‑150 (MQ=255)
                                                                                                    cccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc                                                  >  1:2905935/1‑150 (MQ=255)
                                                                                                      cAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAg                                                >  2:164675/1‑150 (MQ=255)
                                                                                                          catcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTg                                            >  2:1440452/1‑150 (MQ=255)
                                                                                                              aaaaGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGaa                                        >  1:3506933/1‑150 (MQ=255)
                                                                                                               aaaGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGaaa                                       >  1:1698072/1‑150 (MQ=255)
                                                                                                               aaaGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGaaa                                       >  1:1895029/1‑150 (MQ=255)
                                                                                                                atggcgggcgTTACACCGTCACGCTCCGGGATGGCAATAACGTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTAAGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAAc                                      <  2:1246851/148‑1 (MQ=255)
                                                                                                                 cggggggcgTCACACCGACACGCGCCCGTTTGCCACAAAAGTCGAACAACTTAACACCGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACg                                     <  1:4238286/146‑1 (MQ=255)
                                                                                                                   gcgggcgTTACACCGACACGCTCCCGGCTGCCCATAAAGTCCAATAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTg                                   <  2:3033145/150‑1 (MQ=255)
                                                                                                                    cgggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGc                                  >  1:72469/1‑150 (MQ=255)
                                                                                                                    cgggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGc                                  >  1:1617056/1‑150 (MQ=255)
                                                                                                                     gggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGcc                                 >  2:1759089/1‑150 (MQ=255)
                                                                                                                     gggcgTTACACCGTCACGCGCCCGTATGGCACAAAAGTCGACTAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGcc                                 <  2:2386360/150‑1 (MQ=255)
                                                                                                                        cgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCcgcg                              >  1:2366627/1‑150 (MQ=255)
                                                                                                                           tACACCGTCACGCTCCCGGATGGCACTAACGTCGAAGAACTGAACACAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaag                           <  2:271185/150‑1 (MQ=255)
                                                                                                                            acacCGTCACGCTCCCGGATGGCACTAAAGTCTAACAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaaga                          <  1:2247816/150‑1 (MQ=255)
                                                                                                                            acacCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACTGCACCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaaga                          >  1:2406704/1‑150 (MQ=255)
                                                                                                                            acacCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaaga                          >  1:1140501/1‑150 (MQ=255)
                                                                                                                                cgccACGCGCCCGGAGGCCACTAAACCCCACCAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAgg                      <  1:212945/147‑1 (MQ=255)
                                                                                                                                 gTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGt                     >  1:2203167/1‑150 (MQ=255)
                                                                                                                                 gTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGt                     >  2:1013882/1‑150 (MQ=255)
                                                                                                                                     cGCTCCCGTATGCCAAAAAAGTCCAATAACTCAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTa                 <  1:3545386/150‑1 (MQ=255)
                                                                                                                                         cccGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTa             >  2:1858968/1‑150 (MQ=255)
                                                                                                                                            gtaTGGAACAAAAGTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTa          <  2:4031407/148‑1 (MQ=255)
                                                                                                                                             gATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTAc         >  1:2162549/1‑150 (MQ=255)
                                                                                                                                             gATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTAc         >  1:1442557/1‑150 (MQ=255)
                                                                                                                                              aTGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTAcc        >  2:1734555/1‑150 (MQ=255)
                                                                                                                                                ggCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACcac      >  1:3570394/1‑150 (MQ=255)
                                                                                                                                                  cACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACAt    >  1:4227399/1‑150 (MQ=255)
                                                                                                                                                    cTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCa  >  2:1482914/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCA  >  CP009273/1673272‑1673569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑