Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,781,314 T→A 17.9% N19I (AAT→ATT)  ppsA ← phosphoenolpyruvate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,781,3140TA17.9% 663.7 / 131.9 250N19I (AAT→ATT) ppsAphosphoenolpyruvate synthase
Reads supporting (aligned to +/- strand):  ref base T (99/106);  new base A (25/19);  total (124/126)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 275 total aligned reads displayed.

TGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAAT  >  CP009273/1781165‑1781462
                                                                                                                                                     |                                                                                                                                                    
tGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTAcatcat                                                                                                                                                      <  1:4371942/150‑1 (MQ=255)
 gcgcTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACAtcatt                                                                                                                                                     <  1:1213612/150‑1 (MQ=255)
   gcTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGTAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACAtcattca                                                                                                                                                   <  2:3682463/150‑1 (MQ=255)
    cTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACAtcattcat                                                                                                                                                  <  1:278280/150‑1 (MQ=255)
    cTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACAtcattcat                                                                                                                                                  <  2:4225006/150‑1 (MQ=255)
    cTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACAtcattcat                                                                                                                                                  >  1:2939952/1‑150 (MQ=255)
      ggTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGc                                                                                                                                                >  2:4356965/1‑150 (MQ=255)
       gTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGcc                                                                                                                                               <  1:1403929/150‑1 (MQ=255)
       gTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGcc                                                                                                                                               <  1:154524/150‑1 (MQ=255)
              ccgcTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTgg                                                                                                                                        <  2:2516283/150‑1 (MQ=255)
               cgcTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGt                                                                                                                                       >  1:416912/1‑150 (MQ=255)
                gcTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGtt                                                                                                                                      >  1:3510817/1‑150 (MQ=255)
                   ttGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTtata                                                                                                                                   <  1:2025059/150‑1 (MQ=255)
                     ggTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATAcc                                                                                                                                 <  1:3160706/150‑1 (MQ=255)
                         cAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAg                                                                                                                             <  2:2216644/150‑1 (MQ=255)
                         cAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAg                                                                                                                             <  2:4491621/150‑1 (MQ=255)
                           gAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCa                                                                                                                           >  2:343821/1‑150 (MQ=255)
                             aaCTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCAcc                                                                                                                         >  2:1841108/1‑150 (MQ=255)
                                tGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGc                                                                                                                      >  1:60052/1‑150 (MQ=255)
                                tGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGc                                                                                                                      <  1:4349150/150‑1 (MQ=255)
                                tGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGc                                                                                                                      >  2:2809253/1‑150 (MQ=255)
                                   ttAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGt                                                                                                                   <  2:1016977/150‑1 (MQ=255)
                                    tAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTg                                                                                                                  <  2:440704/150‑1 (MQ=255)
                                    tAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTg                                                                                                                  >  1:4027364/1‑150 (MQ=255)
                                     aaaCGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGa                                                                                                                 >  2:2380466/1‑150 (MQ=255)
                                     aaaCGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGa                                                                                                                 <  2:3985556/150‑1 (MQ=255)
                                           gTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGcc                                                                                                           <  1:4500172/150‑1 (MQ=255)
                                            tCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCa                                                                                                          <  1:2315140/150‑1 (MQ=255)
                                             cggcggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAt                                                                                                         <  2:1566738/150‑1 (MQ=255)
                                              ggcggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAtt                                                                                                        >  2:2038224/1‑150 (MQ=255)
                                              ggcggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAtt                                                                                                        <  2:3874502/150‑1 (MQ=255)
                                              ggcggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAtt                                                                                                        >  2:3431550/1‑150 (MQ=255)
                                               gcggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttg                                                                                                       <  2:2611100/150‑1 (MQ=255)
                                                cggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgt                                                                                                      <  1:4327983/150‑1 (MQ=255)
                                                 ggTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgtt                                                                                                     <  2:1922998/150‑1 (MQ=255)
                                                  gTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgttg                                                                                                    >  1:940739/1‑150 (MQ=255)
                                                  gTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgttg                                                                                                    >  1:1543194/1‑150 (MQ=255)
                                                  gTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgttg                                                                                                    <  2:3003155/150‑1 (MQ=255)
                                                    tgtgGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGa                                                                                                  >  2:424049/1‑150 (MQ=255)
                                                      tgGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACa                                                                                                <  1:2953163/150‑1 (MQ=255)
                                                            aaCCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAAc                                                                                          <  2:1583718/150‑1 (MQ=255)
                                                             aCCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACa                                                                                         <  2:681114/150‑1 (MQ=255)
                                                              ccATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACaa                                                                                        <  1:1757852/150‑1 (MQ=255)
                                                                                cccATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGa                                                                      <  1:685375/150‑1 (MQ=255)
                                                                                 ccATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGaa                                                                     <  1:3740324/150‑1 (MQ=255)
                                                                                  cATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAAc                                                                    <  1:1042956/150‑1 (MQ=255)
                                                                                     tCCGGAAAGATTAGTAATCATTTCACCCTGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGt                                                                 >  1:766316/1‑150 (MQ=255)
                                                                                     tCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGt                                                                 <  2:1112302/150‑1 (MQ=255)
                                                                                        ggAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTg                                                              <  2:365387/150‑1 (MQ=255)
                                                                                        ggAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTg                                                              >  2:4153700/1‑150 (MQ=255)
                                                                                        ggAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTg                                                              >  2:1413007/1‑150 (MQ=255)
                                                                                         gAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTga                                                             >  2:579843/1‑150 (MQ=255)
                                                                                           aaGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTgaga                                                           >  2:2959088/1‑150 (MQ=255)
                                                                                           aaGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTgaga                                                           >  1:3966399/1‑150 (MQ=255)
                                                                                            aGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGaa                                                          >  2:4086403/1‑150 (MQ=255)
                                                                                            aGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGaa                                                          >  2:3728066/1‑150 (MQ=255)
                                                                                            aGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGaa                                                          >  2:2511721/1‑150 (MQ=255)
                                                                                              aTTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAac                                                        >  2:2896820/1‑150 (MQ=255)
                                                                                              aTTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAac                                                        >  1:3097288/1‑150 (MQ=255)
                                                                                               ttAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAaca                                                       <  2:468834/150‑1 (MQ=255)
                                                                                               ttAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAaca                                                       <  2:1134011/150‑1 (MQ=255)
                                                                                                tAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAacac                                                      >  2:3175988/1‑150 (MQ=255)
                                                                                                    aaTCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACAttt                                                  >  1:1965257/1‑150 (MQ=255)
                                                                                                       cATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTg                                               >  2:3307142/1‑150 (MQ=255)
                                                                                                       cATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTg                                               <  2:1808929/150‑1 (MQ=255)
                                                                                                        aTTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTgc                                              >  1:2891908/1‑150 (MQ=255)
                                                                                                         tttCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTgcg                                             <  2:1744689/150‑1 (MQ=255)
                                                                                                          ttCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTgcgc                                            >  1:327346/1‑150 (MQ=255)
                                                                                                              cccAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCAttt                                        <  2:3693122/150‑1 (MQ=255)
                                                                                                               ccAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTa                                       >  2:4478072/1‑150 (MQ=255)
                                                                                                               ccAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTa                                       >  1:2131077/1‑150 (MQ=255)
                                                                                                                cAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTAt                                      >  1:4080347/1‑150 (MQ=255)
                                                                                                                 aGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATc                                     >  1:3417239/1‑150 (MQ=255)
                                                                                                                  gggAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCt                                    >  2:1719102/1‑150 (MQ=255)
                                                                                                                    gAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCttt                                  <  1:352950/150‑1 (MQ=255)
                                                                                                                    gAGGCATTTTTGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCttt                                  <  2:2358990/150‑1 (MQ=255)
                                                                                                                        cATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCat                              <  1:1899525/150‑1 (MQ=255)
                                                                                                                         aTTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCata                             <  2:2082794/150‑1 (MQ=255)
                                                                                                                         aTTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCata                             >  1:3845354/1‑150 (MQ=255)
                                                                                                                             ttGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTa                         <  1:1635375/150‑1 (MQ=255)
                                                                                                                              tGCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTaa                        >  1:306227/1‑150 (MQ=255)
                                                                                                                               gCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAAc                       >  2:2631232/1‑150 (MQ=255)
                                                                                                                               gCCCCCAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAAc                       <  1:2411470/150‑1 (MQ=255)
                                                                                                                                cccccAACCCTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAAcc                      <  1:3341162/150‑1 (MQ=255)
                                                                                                                                     aaCCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATg                 >  1:2988127/1‑150 (MQ=255)
                                                                                                                                      aCCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGa                <  2:1544191/150‑1 (MQ=255)
                                                                                                                                      aCCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGa                <  1:61226/150‑1 (MQ=255)
                                                                                                                                        ccTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGaaa              <  1:2399806/150‑1 (MQ=255)
                                                                                                                                        ccTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGaaa              <  1:134368/150‑1 (MQ=255)
                                                                                                                                         cTGTCTACATCAATCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGaaaa             <  1:4114473/150‑1 (MQ=255)
                                                                                                                                          tGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGaaaaa            >  2:160988/1‑150 (MQ=255)
                                                                                                                                           gTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGaaaaaa           >  1:1634693/1‑150 (MQ=255)
                                                                                                                                                catcatTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGt      >  2:1378985/1‑150 (MQ=255)
                                                                                                                                                  tcattcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGa    >  1:10230/1‑150 (MQ=255)
                                                                                                                                                  tcattcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGa    >  1:10018/1‑150 (MQ=255)
                                                                                                                                                   cattcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGaa   <  1:276335/150‑1 (MQ=255)
                                                                                                                                                    attcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAAt  >  2:2460313/1‑150 (MQ=255)
                                                                                                                                                    attcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAAt  >  2:2292320/1‑150 (MQ=255)
                                                                                                                                                    attcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAAt  <  1:3786410/150‑1 (MQ=255)
                                                                                                                                                    attcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAAt  >  2:856201/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCATATTTAACCGGATGAAAAAAACGGTGAAT  >  CP009273/1781165‑1781462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑