BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,784,363	C→A	13.0%	R380L (CGT→CTT)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,784,363	0	C	A	13.0%	1011.8 / 89.4	301	R380L (CGT→CTT)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand): ref base C (125/136); new base A (20/20); total (145/156)
Fisher's exact test for biased strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

GTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCT  >  CP009273/1784214‑1784510

gTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAc                                                                                                                                                     >  1:891285/1‑150 (MQ=255)

gTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAc                                                                                                                                                     >  1:3225014/1‑150 (MQ=255)

 ttGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACg                                                                                                                                                    <  2:3632899/150‑1 (MQ=255)

  tGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGt                                                                                                                                                   <  2:121252/150‑1 (MQ=255)

    cgcCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAg                                                                                                                                                 <  2:2662269/150‑1 (MQ=255)

    cgcCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAg                                                                                                                                                 <  1:1736840/150‑1 (MQ=255)

       cAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCgg                                                                                                                                              >  2:177676/1‑150 (MQ=255)

       cAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCgg                                                                                                                                              >  2:680862/1‑150 (MQ=255)

           cAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAc                                                                                                                                          >  2:2879595/1‑150 (MQ=255)

           cAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGAc                                                                                                                                          <  1:3398786/150‑1 (MQ=255)

            aGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAcg                                                                                                                                         <  1:2593952/150‑1 (MQ=255)

            aGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAAGAAACTCATCACGTAGCGGTGAcg                                                                                                                                         >  1:575806/1‑150 (MQ=255)

              ttGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGAcgcc                                                                                                                                       <  1:1421664/150‑1 (MQ=255)

                    aaTACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCtg                                                                                                                                 >  2:3491745/1‑150 (MQ=255)

                       aCCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTAACGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGtgc                                                                                                                              >  1:4485883/1‑150 (MQ=255)

                            agCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTc                                                                                                                         <  1:2455313/150‑1 (MQ=255)

                               ggggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                                                                                                      >  1:4001618/1‑150 (MQ=255)

                               ggggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                                                                                                      <  1:4198996/150‑1 (MQ=255)

                               ggggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                                                                                                      >  1:3862224/1‑150 (MQ=255)

                               ggggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                                                                                                      >  1:2438089/1‑150 (MQ=255)

                               ggggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                                                                                                      >  1:1580601/1‑150 (MQ=255)

                                  gTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAg                                                                                                                   >  2:3734471/1‑150 (MQ=255)

                                       cGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCa                                                                                                              <  2:1867547/150‑1 (MQ=255)

                                       cGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCa                                                                                                              <  2:3200648/150‑1 (MQ=255)

                                        gCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAg                                                                                                             >  1:3928086/1‑150 (MQ=255)

                                          tttGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCa                                                                                                           >  2:1412310/1‑150 (MQ=255)

                                            tGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATg                                                                                                         >  1:2950155/1‑150 (MQ=255)

                                                  aGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAt                                                                                                   >  1:3636695/1‑150 (MQ=255)

                                                   gTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtg                                                                                                  <  1:2847692/150‑1 (MQ=255)

                                                   gTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtg                                                                                                  <  1:512861/150‑1 (MQ=255)

                                                       cTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCg                                                                                              >  2:2335753/1‑150 (MQ=255)

                                                       cTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCg                                                                                              >  2:3693370/1‑150 (MQ=255)

                                                        tGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCgg                                                                                             <  2:4142289/150‑1 (MQ=255)

                                                           cGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGta                                                                                          <  2:1838776/150‑1 (MQ=255)

                                                            gCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGtat                                                                                         <  1:2044420/150‑1 (MQ=255)

                                                               cACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGACTGTGCGGGTATAAt                                                                                      >  1:2145820/1‑150 (MQ=255)

                                                               cACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAAt                                                                                      <  1:2587251/150‑1 (MQ=255)

                                                                aCTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATc                                                                                     >  1:3470437/1‑150 (MQ=255)

                                                                 cTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCg                                                                                    <  1:464600/150‑1 (MQ=255)

                                                                  tATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGACTGTGCGGGTAGAATCGc                                                                                   >  2:86337/1‑150 (MQ=255)

                                                                  tATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGc                                                                                   >  1:3298312/1‑150 (MQ=255)

                                                                  tATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGc                                                                                   >  1:2891088/1‑150 (MQ=255)

                                                                    tCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTg                                                                                 <  2:4164579/150‑1 (MQ=255)

                                                                        tAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGct                                                                             >  1:1994974/1‑150 (MQ=255)

                                                                        tAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGct                                                                             <  2:2734044/150‑1 (MQ=255)

                                                                         aaCCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGctc                                                                            >  1:4069144/1‑150 (MQ=255)

                                                                          aCCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGctct                                                                           >  1:412396/1‑150 (MQ=255)

                                                                          aCCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGctct                                                                           <  2:3237654/150‑1 (MQ=255)

                                                                            cTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg                                                                         <  1:444953/150‑1 (MQ=255)

                                                                               gTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGACTGTGCGGGTATAATCGCTGCGCTCTCgcgc                                                                      >  2:3423973/1‑150 (MQ=255)

                                                                               gTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCgcgc                                                                      >  1:230706/1‑150 (MQ=255)

                                                                                 cTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCa                                                                    <  1:1542248/150‑1 (MQ=255)

                                                                                   tgttgCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATc                                                                  >  1:817985/1‑150 (MQ=255)

                                                                                   tgttgCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATc                                                                  >  1:160903/1‑150 (MQ=255)

                                                                                    gttgCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCa                                                                 >  2:3288646/1‑150 (MQ=255)

                                                                                       gCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGAc                                                              <  2:2467161/150‑1 (MQ=255)

                                                                                        cAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACt                                                             >  1:3826471/1‑150 (MQ=255)

                                                                                         aaaCGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTg                                                            >  1:2658464/1‑150 (MQ=255)

                                                                                         aaaCGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTg                                                            <  1:1632595/150‑1 (MQ=255)

                                                                                         aaaCGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTg                                                            <  2:4485883/150‑1 (MQ=255)

                                                                                           aCGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGaa                                                          >  2:3095344/1‑150 (MQ=255)

                                                                                                 ccGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCACGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattc                                                    <  1:1874539/150‑1 (MQ=255)

                                                                                                  cGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattca                                                   >  2:2772354/1‑150 (MQ=255)

                                                                                                   gATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcat                                                  >  2:1042549/1‑150 (MQ=255)

                                                                                                   gATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcat                                                  >  1:1693824/1‑150 (MQ=255)

                                                                                                   gATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcat                                                  <  2:2002309/150‑1 (MQ=255)

                                                                                                    aTAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcatt                                                 >  2:1674145/1‑150 (MQ=255)

                                                                                                    aTAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcatt                                                 >  1:1039551/1‑150 (MQ=255)

                                                                                                     tAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcattc                                                >  2:1753126/1‑150 (MQ=255)

                                                                                                        cGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCTCGCCATCAGACTGAATAATTCATTCAGt                                             <  1:1226633/150‑1 (MQ=255)

                                                                                                         gggCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTa                                            <  1:309839/150‑1 (MQ=255)

                                                                                                              aaCCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGcgcg                                       <  2:2390539/150‑1 (MQ=255)

                                                                                                                ccAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGtt                                     <  1:928960/150‑1 (MQ=255)

                                                                                                                 cAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTa                                    >  1:2239437/1‑150 (MQ=255)

                                                                                                                 cAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTa                                    <  2:3754871/150‑1 (MQ=255)

                                                                                                                 cAGTCATCAAATGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTa                                    <  2:1472570/150‑1 (MQ=255)

                                                                                                                  aGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTAt                                   >  2:421604/1‑150 (MQ=255)

                                                                                                                   gTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATc                                  <  1:3348525/150‑1 (MQ=255)

                                                                                                                      atcaAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCctc                               >  2:2103840/1‑150 (MQ=255)

                                                                                                                         aaaTGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCttt                            >  2:4327756/1‑150 (MQ=255)

                                                                                                                         aaaTGCCGCACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCttt                            >  2:2405681/1‑150 (MQ=255)

                                                                                                                          aaTGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCtttt                           <  2:2260544/150‑1 (MQ=255)

                                                                                                                           aTGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCttttt                          <  2:589850/150‑1 (MQ=255)

                                                                                                                               cGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTc                      >  1:3771393/1‑150 (MQ=255)

                                                                                                                                gCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTcc                     <  2:1529975/150‑1 (MQ=255)

                                                                                                                                 cACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCg                    >  1:3137509/1‑150 (MQ=255)

                                                                                                                                 cACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCg                    <  1:1597480/150‑1 (MQ=255)

                                                                                                                                 cACGATCAATAAACTCATCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCg                    >  1:3525868/1‑150 (MQ=255)

                                                                                                                                    gATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCa                 <  1:2490853/150‑1 (MQ=255)

                                                                                                                                      tCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATg               <  2:3950525/150‑1 (MQ=255)

                                                                                                                                        aaTAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGaa             >  2:2138049/1‑150 (MQ=255)

                                                                                                                                          aaaaCTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGc           <  2:1800761/149‑1 (MQ=255)

                                                                                                                                           aaaCTCCTCAAGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGcc          <  1:152912/150‑1 (MQ=255)

                                                                                                                                             aCTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCa        <  2:4057884/150‑1 (MQ=255)

                                                                                                                                             aCTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCa        <  1:4365522/150‑1 (MQ=255)

                                                                                                                                              cTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAg       >  2:4402555/1‑150 (MQ=255)

                                                                                                                                                  tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTc   <  2:1018410/150‑1 (MQ=255)

                                                                                                                                                   caCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCt  <  2:3826471/150‑1 (MQ=255)

                                                                                                                                                   caCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCt  <  2:2658464/150‑1 (MQ=255)

                                                                                                                                                   caCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCt  >  2:2568837/1‑150 (MQ=255)

GTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGGCAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCT  >  CP009273/1784214‑1784510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`