Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,835 A→C 7.3% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8350AC 7.3% 781.5 / 3.7 327W53G (TGG→GGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (200/88);  new base C (2/34);  total (202/125)
Fisher's exact test for biased strand distribution p-value = 4.39e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

GATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATA  >  CP009273/1823686‑1823984
                                                                                                                                                     |                                                                                                                                                     
    atcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcgc                                                                                                                                                   >  1:3366548/1‑150 (MQ=255)
    atcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcgc                                                                                                                                                   >  2:2150269/1‑150 (MQ=255)
     tcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGcgcg                                                                                                                                                  >  1:2395981/1‑150 (MQ=255)
      cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                 >  2:4492259/1‑150 (MQ=255)
         cGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                                                              <  1:182344/150‑1 (MQ=255)
             aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                                                          >  2:1591604/1‑150 (MQ=255)
                tCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCa                                                                                                                                       >  2:4517509/1‑150 (MQ=255)
                  ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                                                     >  1:2082034/1‑150 (MQ=255)
                   gTTGCCGGTTCCCCGCGCGGTTTCCCGTTTTCTGTGGAAATAATCGGGTTTAATTGGGCTTTTTTGTTTTCCAGCTGTGCGGAAAAGGGTTCGACACCGGATTTAGGTTCAGAAATTGAATGCCGCGCCCAGCGCGGAAACGCCGCACgg                                                                                                                                    <  2:1277632/150‑1 (MQ=255)
                   gTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACgg                                                                                                                                    >  1:790951/1‑150 (MQ=255)
                        cGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGc                                                                                                                               >  2:2709764/1‑150 (MQ=255)
                         gCTTCCCAGCGCGGGTTCCCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCTGCAGTGCGGCAAAGCGTTCGACAACGGAATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGcc                                                                                                                              <  2:3721502/150‑1 (MQ=255)
                         gCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGcc                                                                                                                              >  1:1709864/1‑150 (MQ=255)
                          cTTCCCCGCGGGGATTACCCGTTTCTCTGGAAATAATCGCGTTTAATTCGGATTTATTGCTTTCCAGCTGGGGGGAAAAGCTTGCGAAACGGGAATGACTTTCAGCAATTGAACGCCGCGCCCTGCGGGGAAACGCCGCACGGGCTGccc                                                                                                                             <  2:1944498/150‑1 (MQ=255)
                           ttCCCCGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                                                            <  2:2627020/150‑1 (MQ=255)
                           ttCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                                                            >  1:2187461/1‑150 (MQ=255)
                                   gcgGCTTACCCGTTTCTGGGGCAATAAGCGCGGTTAATTCGGCTTTTTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACACCGGAATTAGGTTCAGCAATTTAAAGCCGCGCCCAGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCt                                                                                                                    <  2:3488868/150‑1 (MQ=255)
                                    cgGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTg                                                                                                                   >  1:3425962/1‑150 (MQ=255)
                                      gCTTACCGTTTTCTCGGGCAATAATCGCGTTTATTTGGGCTTTTTTGCTTTCCAGCAGTGCGGCAACGCGTTCGACACGGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCt                                                                                                                 <  2:826604/150‑1 (MQ=255)
                                      gCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCt                                                                                                                 >  1:1370163/1‑150 (MQ=255)
                                       cTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCACATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTc                                                                                                                >  2:2125157/1‑150 (MQ=255)
                                       cTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTc                                                                                                                >  1:2535966/1‑150 (MQ=255)
                                        ttACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCTGCACGGGCTGCCCGACAAGCCTGCTCg                                                                                                               <  2:3558494/150‑1 (MQ=255)
                                        ttACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCg                                                                                                               >  1:3943160/1‑150 (MQ=255)
                                           cccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAcc                                                                                                            >  1:3579899/1‑150 (MQ=255)
                                            ctgTTTCTCTGGAAAAAACCGCGTTTAATTCGTCTTTATTGCTTCCCCGCTGTGCGGAAACGCGTTCGACACCGGCATGAGTTACAGAAAATCAAACCCGCCCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                           <  1:1034080/148‑1 (MQ=255)
                                            cggTTTCTTTGGAAATAATCGCGTTTATTTCGTATTTATTGCTTTCCAGCTGTGCGGAAAAGCGTTCTAAACCGGAATGACGTTCAGAAAATGAAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                           <  1:380574/148‑1 (MQ=255)
                                              gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                                                         >  1:210397/1‑150 (MQ=255)
                                                tcctggggCAATAATCGCGGTTAATGCGGCTTTTTTGCTTTCCAGCAGTGCGGCAATGCGTGCGACACCGGAATGACGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGc                                                                                                       <  2:3858243/144‑1 (MQ=255)
                                                 tctcTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                      >  1:2895613/1‑150 (MQ=255)
                                                 tctcTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                      >  1:822899/1‑150 (MQ=255)
                                                 tctcTGGCAATAATCGCGGTTAATTCGGATTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                      <  2:1787602/150‑1 (MQ=255)
                                                  ctctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcgg                                                                                                     >  2:369551/1‑150 (MQ=255)
                                                     tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAACCGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                                                                  >  2:2419084/1‑150 (MQ=255)
                                                     tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                                                                  >  2:2270204/1‑150 (MQ=255)
                                                         aataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                                              >  1:1148241/1‑150 (MQ=255)
                                                           taagCGCGGTTAATGCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCTTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                            <  2:2366006/150‑1 (MQ=255)
                                                             atCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                                                                          >  1:2011861/1‑150 (MQ=255)
                                                              tCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                                                         >  2:3607254/1‑150 (MQ=255)
                                                               cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAACGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                        <  2:1095234/150‑1 (MQ=255)
                                                               cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                        >  1:446757/1‑150 (MQ=255)
                                                               cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                        >  2:1364448/1‑150 (MQ=255)
                                                                  ggTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcc                                                                                     >  1:501092/1‑150 (MQ=255)
                                                                  ggTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcc                                                                                     >  1:4321241/1‑150 (MQ=255)
                                                                     tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                                                  >  1:996743/1‑150 (MQ=255)
                                                                     tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                                                  >  2:119977/1‑150 (MQ=255)
                                                                          cGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATa                                                                             >  1:752618/1‑150 (MQ=255)
                                                                              tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAacac                                                                         >  1:2456350/1‑150 (MQ=255)
                                                                               ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                                                        <  2:2583589/150‑1 (MQ=255)
                                                                               ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                                                        >  1:3631325/1‑150 (MQ=255)
                                                                                 tttGCTTTCCAGCTGTGCGGAAAAGCGTTCGACACCGGCATGACGTACAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTc                                                                      <  1:2826802/149‑1 (MQ=255)
                                                                                 aTTGCTTTCCAGCTGTGCGGAAAAGCGTTCGACACCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTc                                                                      <  1:2988982/150‑1 (MQ=255)
                                                                                  ttGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                                                                     >  2:4427283/1‑150 (MQ=255)
                                                                                       ttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGa                                                                >  2:2989697/1‑150 (MQ=255)
                                                                                        tCCAGCTGTGCGGCAAAGCGTTCGACACCGGAATGACGTCCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                                                               <  1:1875005/150‑1 (MQ=255)
                                                                                        tCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                                                               >  1:3246986/1‑150 (MQ=255)
                                                                                          ctgctgTGCGGCAACGCGTTCGACACCGGAATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                                                             <  1:4071555/145‑1 (MQ=255)
                                                                                          cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAGTGCCTTGCCATAACACCTCGCCCGATAc                                                             >  2:757746/1‑150 (MQ=255)
                                                                                           agctgTGCGGAAAAGTGTTCGACACCGGATTGAGGTTCAGCAAATCAACGCCGCGCCCCGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                            <  1:488907/150‑1 (MQ=255)
                                                                                           agccgTGCGGAAAAGCGTACGACAACGGCATGACGTACACCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                            <  1:1410249/150‑1 (MQ=255)
                                                                                             cagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg                                                          >  2:505948/1‑150 (MQ=255)
                                                                                                 gCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa                                                      <  2:3298256/150‑1 (MQ=255)
                                                                                                 gCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa                                                      <  2:2082034/150‑1 (MQ=255)
                                                                                                 gCGGAAACGCGTTCGACACCGGCATGCCGTTCACCAAATGACCGCCGCGCCCCGCGGGTACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTa                                                      <  1:3129746/150‑1 (MQ=255)
                                                                                                   ggCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACg                                                    >  2:1228140/1‑150 (MQ=255)
                                                                                                     cAAAGCTTTCGACACCGGCATGACGTTCAGCAAATTAACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                  <  2:4191840/150‑1 (MQ=255)
                                                                                                     cAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                  <  2:1447858/150‑1 (MQ=255)
                                                                                                      aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt                                                 >  2:902177/1‑150 (MQ=255)
                                                                                                        aGCGTTCGACACCGGCATGACGTTCAGCAAATTAACGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTca                                               <  2:2734384/150‑1 (MQ=255)
                                                                                                         gCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcac                                              >  2:2902149/1‑150 (MQ=255)
                                                                                                          cGTTCGACAACGGCATGACGTTCAGCAATTGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                             <  2:3047882/150‑1 (MQ=255)
                                                                                                          cGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                             >  1:902026/1‑150 (MQ=255)
                                                                                                           gTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCacac                                            >  1:3042365/1‑150 (MQ=255)
                                                                                                           gTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCacac                                            >  1:587809/1‑150 (MQ=255)
                                                                                                                 cAACGGCATGACGTTCAGCAAATTAAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                      <  1:2242362/150‑1 (MQ=255)
                                                                                                                 cAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                      >  2:1404583/1‑150 (MQ=255)
                                                                                                                  acgggCTTGACGTTCAGAAACGGACCGCCGCGCCCCGCGCGGCCACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAGTACGCTTCACACGCGATgc                                     <  2:787296/147‑1 (MQ=255)
                                                                                                                   aCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgcg                                    <  2:1647747/150‑1 (MQ=255)
                                                                                                                     ggCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCAGATACCGGATTACGCTTCACACGCGATGCGcc                                  >  2:1046493/1‑150 (MQ=255)
                                                                                                                       taTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCt                                >  2:1679007/2‑150 (MQ=255)
                                                                                                                       cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCt                                >  2:3782140/1‑150 (MQ=255)
                                                                                                                         tGACGTTCAGCAAATGAATGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTgg                              <  1:1099050/150‑1 (MQ=255)
                                                                                                                            cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGccc                           >  1:1285246/1‑150 (MQ=255)
                                                                                                                             gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCg                          >  1:1314419/1‑150 (MQ=255)
                                                                                                                             gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCg                          >  1:1936598/1‑150 (MQ=255)
                                                                                                                             gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTACACACGCGATGCGCCCTGGCCCg                          >  1:3702409/1‑150 (MQ=255)
                                                                                                                               tCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGtt                        <  2:2487359/150‑1 (MQ=255)
                                                                                                                               acAGCAAATTAACGCCGCGCCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGtt                        <  1:1639797/149‑1 (MQ=255)
                                                                                                                                cAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                       >  1:1080888/1‑150 (MQ=255)
                                                                                                                                   cAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTACCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATcc                    >  2:4129532/1‑150 (MQ=255)
                                                                                                                                   cAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTACCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATcc                    >  2:3291429/1‑150 (MQ=255)
                                                                                                                                    aattGAAAGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   <  1:155342/147‑1 (MQ=255)
                                                                                                                                     actGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                  <  2:3947622/148‑1 (MQ=255)
                                                                                                                                        gAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCa               <  2:3182474/150‑1 (MQ=255)
                                                                                                                                         aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc              >  2:685132/1‑150 (MQ=255)
                                                                                                                                         aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc              >  2:2541858/1‑150 (MQ=255)
                                                                                                                                             ccGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGtt          <  2:642597/150‑1 (MQ=255)
                                                                                                                                              cgcgCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTa         <  2:3366548/150‑1 (MQ=255)
                                                                                                                                               gcgcCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTaa        <  2:2678190/150‑1 (MQ=255)
                                                                                                                                                  cccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc     <  2:3203857/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATA  >  CP009273/1823686‑1823984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑