Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,848,731 C→A 15.4% A127A (GCG→GCT)  ydjF ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,848,7310CA15.4% 662.8 / 78.4 212A127A (GCG→GCT) ydjFputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (87/92);  new base A (21/12);  total (108/104)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TCAAGACCTTTACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTC  >  CP009273/1848582‑1848880
                                                                                                                                                     |                                                                                                                                                     
tCAAGACCTTTACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATc                                                                                                                                                       <  2:2010429/150‑1 (MQ=255)
   aGACCTTTACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCt                                                                                                                                                    <  2:3777574/150‑1 (MQ=255)
      ccTTTACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTcc                                                                                                                                                 <  1:1727926/150‑1 (MQ=255)
          tACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTccgccg                                                                                                                                             <  1:570233/150‑1 (MQ=255)
          tACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTccgccg                                                                                                                                             <  1:2432696/150‑1 (MQ=255)
          gaCAACTCATTACCATGATATCGACATGATAGCGCCTGATTATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTccgccg                                                                                                                                             <  2:4116109/149‑1 (MQ=255)
             aaCTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGt                                                                                                                                          >  2:754259/1‑150 (MQ=255)
             aaCTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGt                                                                                                                                          <  2:1707618/150‑1 (MQ=255)
              aCTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGtt                                                                                                                                         <  2:3148791/150‑1 (MQ=255)
                  aTTACCATTATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGtt                                                                                                                                     <  1:1601791/150‑1 (MQ=255)
                  aTTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGtt                                                                                                                                     >  2:3170157/1‑150 (MQ=255)
                     aCCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTTCAGTGAAAGTGTATATTTGTTTAGTTACCCACCCGTTGAAACGACTATAATTTCTGACTGAGCCAGAACATGAATCTCTTCCGCCGAGTTGGATAGc                                                                                                                                  >  1:2262886/1‑150 (MQ=255)
                     aCCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGc                                                                                                                                  >  1:2482478/1‑150 (MQ=255)
                        aTGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAAc                                                                                                                               <  1:275719/150‑1 (MQ=255)
                        aTGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAAc                                                                                                                               >  1:2135200/1‑150 (MQ=255)
                         tGATATCGACATGATCGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGGGAAAGTGTATGGTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACg                                                                                                                              >  2:18166/1‑150 (MQ=255)
                          tatatCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAATGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGt                                                                                                                             <  1:3128446/149‑1 (MQ=255)
                            tatCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCa                                                                                                                           >  1:319032/1‑150 (MQ=255)
                             atCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAg                                                                                                                          >  1:2902791/1‑150 (MQ=255)
                             atCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAg                                                                                                                          <  1:82955/150‑1 (MQ=255)
                                gACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGcc                                                                                                                       <  1:124917/150‑1 (MQ=255)
                                gACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGcc                                                                                                                       >  2:4454618/1‑150 (MQ=255)
                                  cATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCAc                                                                                                                     <  2:2863123/150‑1 (MQ=255)
                                    tGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTa                                                                                                                   >  1:1539819/1‑150 (MQ=255)
                                     gATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTAc                                                                                                                  >  1:2127889/1‑150 (MQ=255)
                                      aTAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACg                                                                                                                 <  2:1864610/150‑1 (MQ=255)
                                        aGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGt                                                                                                               >  1:4401371/1‑150 (MQ=255)
                                              tgatgaTCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTa                                                                                                         <  1:2213702/150‑1 (MQ=255)
                                                atgatCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAAc                                                                                                       >  1:1655252/1‑150 (MQ=255)
                                                 tgatCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACa                                                                                                      >  2:4357357/1‑150 (MQ=255)
                                                      tctTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTg                                                                                                 <  2:1085178/150‑1 (MQ=255)
                                                         ttAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGc                                                                                              >  2:37544/1‑150 (MQ=255)
                                                            gTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAAt                                                                                           <  1:2632498/150‑1 (MQ=255)
                                                              aaTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTc                                                                                         >  1:607826/1‑150 (MQ=255)
                                                               aTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTcc                                                                                        <  1:1754543/150‑1 (MQ=255)
                                                               aTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTcc                                                                                        <  2:1502202/150‑1 (MQ=255)
                                                               aTTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTcc                                                                                        <  1:3427810/150‑1 (MQ=255)
                                                                 tcttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCAt                                                                                      >  2:1658071/1‑150 (MQ=255)
                                                                 tcttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCAt                                                                                      <  2:827080/150‑1 (MQ=255)
                                                                 tcttcCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCAt                                                                                      >  1:4280498/1‑150 (MQ=255)
                                                                      cTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACgg                                                                                 <  2:1371066/150‑1 (MQ=255)
                                                                      cTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACgg                                                                                 >  1:507863/1‑150 (MQ=255)
                                                                      cTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACgg                                                                                 >  2:2308701/1‑150 (MQ=255)
                                                                      cTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACgg                                                                                 <  2:4510413/150‑1 (MQ=255)
                                                                      cTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACgg                                                                                 >  1:2357096/1‑150 (MQ=255)
                                                                      cTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACgg                                                                                 >  1:593100/1‑150 (MQ=255)
                                                                           aGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGtacta                                                                            >  2:3439227/1‑150 (MQ=255)
                                                                             tGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTactact                                                                          >  1:3234252/1‑150 (MQ=255)
                                                                                aaGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGa                                                                       <  2:1959841/150‑1 (MQ=255)
                                                                                   tgtATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATc                                                                    <  1:2155748/150‑1 (MQ=255)
                                                                                   tgtATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATc                                                                    <  1:3360840/150‑1 (MQ=255)
                                                                                   tgtATTTTTGTTTAGGTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATc                                                                    <  2:2724655/150‑1 (MQ=255)
                                                                                     tATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACATTTTGAGCAATTCCATTACGGTACTACTGGAATCgg                                                                  <  2:2902791/150‑1 (MQ=255)
                                                                                      aTTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGc                                                                 >  1:2588216/1‑150 (MQ=255)
                                                                                         tttGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGACTCGGCTGc                                                              >  1:3719825/1‑150 (MQ=255)
                                                                                         tttGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGc                                                              >  1:779816/1‑150 (MQ=255)
                                                                                           tGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCa                                                            >  2:3584226/1‑150 (MQ=255)
                                                                                            gTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCAt                                                           >  1:3461444/1‑150 (MQ=255)
                                                                                             tttAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCAtg                                                          >  2:673957/1‑150 (MQ=255)
                                                                                              ttAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCAtgg                                                         <  1:4005558/150‑1 (MQ=255)
                                                                                                aGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCAtggtg                                                       >  2:3938493/1‑150 (MQ=255)
                                                                                                   tCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGtt                                                    <  2:3771647/150‑1 (MQ=255)
                                                                                                     cccACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGtttt                                                  >  1:1202630/1‑150 (MQ=255)
                                                                                                      ccACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTa                                                 >  1:204927/1‑150 (MQ=255)
                                                                                                       cACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTAt                                                >  1:3490261/1‑150 (MQ=255)
                                                                                                          ccGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGt                                             <  1:1888286/150‑1 (MQ=255)
                                                                                                            gTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCg                                           >  1:1298976/1‑150 (MQ=255)
                                                                                                            gTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCg                                           <  2:1794239/150‑1 (MQ=255)
                                                                                                            gTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCg                                           <  1:2241541/150‑1 (MQ=255)
                                                                                                            gTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCg                                           <  1:3580722/150‑1 (MQ=255)
                                                                                                             ttGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGa                                          <  1:3886788/150‑1 (MQ=255)
                                                                                                             ttGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCAGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGa                                          <  2:2119466/150‑1 (MQ=255)
                                                                                                              ttaaaCGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGAt                                         <  1:410794/148‑1 (MQ=255)
                                                                                                              tGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGAt                                         <  1:3604588/150‑1 (MQ=255)
                                                                                                               gAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATa                                        >  1:2033942/1‑150 (MQ=255)
                                                                                                                 aaCGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATaaa                                      >  1:1284653/1‑150 (MQ=255)
                                                                                                                  aCGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAg                                     <  2:3157102/150‑1 (MQ=255)
                                                                                                                   cGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAgg                                    <  1:106898/150‑1 (MQ=255)
                                                                                                                   cGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAgg                                    <  1:2375824/150‑1 (MQ=255)
                                                                                                                   cGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAgg                                    <  1:4535277/150‑1 (MQ=255)
                                                                                                                    gACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAggg                                   >  1:535339/1‑150 (MQ=255)
                                                                                                                          aaTTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGc                             >  1:1444111/1‑150 (MQ=255)
                                                                                                                           aTTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGcc                            >  1:1490713/1‑150 (MQ=255)
                                                                                                                                 gactgaGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGt                      <  1:2776618/150‑1 (MQ=255)
                                                                                                                                   ctgaGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGc                    <  2:4170533/150‑1 (MQ=255)
                                                                                                                                   ctgaGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGc                    <  2:4170342/150‑1 (MQ=255)
                                                                                                                                    tgaGCCAGAACATGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCa                   <  2:296824/150‑1 (MQ=255)
                                                                                                                                      aGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAAt                 <  1:156132/150‑1 (MQ=255)
                                                                                                                                       gCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATa                <  1:3931126/150‑1 (MQ=255)
                                                                                                                                        ccAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATaa               <  2:3834388/150‑1 (MQ=255)
                                                                                                                                            aaCATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAgctg           >  1:2661938/1‑150 (MQ=255)
                                                                                                                                            aaCATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAgctg           <  1:4454636/150‑1 (MQ=255)
                                                                                                                                            aaCATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAgctg           >  1:1967014/1‑150 (MQ=255)
                                                                                                                                            aaCATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAgctg           >  1:4244968/1‑150 (MQ=255)
                                                                                                                                            aaCATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAgctg           <  1:2449700/150‑1 (MQ=255)
                                                                                                                                              cATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAgctgct         >  1:661364/1‑150 (MQ=255)
                                                                                                                                               aTGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCtt        >  1:2679807/1‑150 (MQ=255)
                                                                                                                                               aTGAATAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCtt        <  1:1345949/150‑1 (MQ=255)
                                                                                                                                                  aaTCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTtctc     >  2:558722/1‑150 (MQ=255)
                                                                                                                                                  aaTAGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTtctc     >  1:3945613/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAAGACCTTTACAACTCATTACCATGATATCGACATGATAGCGCCTGATGATCTCTTTAGTAATTCTTCCTTGCAGTGAAAGTGTATTTTTGTTTAGTTCCCCACCCGTTGAAACGACTTTAATTTCTGACTGAGCCAGAACATGAATCGCTTCCGCCGAGTTGGTTAGCAACGTCAGGCCACTACGGTCCTGTAACAATTTGAGCAATTCCATTACGGTACTACTGGAATCGGCTGCCATGGTGGTTTTATTGTCGATAAAGGGTAGTGCCTTGCGTGCAATAAGCTGCTTCTCTTC  >  CP009273/1848582‑1848880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑