Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,959,915 C→A 6.8% P178P (CCG→CCT)  flhB ← flagellin export apparatus, substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,959,9150CA 6.8% 1082.7 / 37.3 287P178P (CCG→CCT) flhBflagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (151/116);  new base A (12/8);  total (163/124)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  CP009273/1959767‑1960064
                                                                                                                                                    |                                                                                                                                                     
caCGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCg                                                                                                                                                      <  1:115427/150‑1 (MQ=255)
caCGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCg                                                                                                                                                      >  2:3223936/1‑150 (MQ=255)
  cGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGga                                                                                                                                                    <  2:1385271/150‑1 (MQ=255)
   gAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGgag                                                                                                                                                   >  1:570433/1‑150 (MQ=255)
    aaTATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGgaga                                                                                                                                                  >  2:4371444/1‑150 (MQ=255)
       atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTc                                                                                                                                               >  1:3307180/1‑150 (MQ=255)
       atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTc                                                                                                                                               >  1:1716854/1‑150 (MQ=255)
        tCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCg                                                                                                                                              <  1:3608052/150‑1 (MQ=255)
              cGTGACATACTCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATc                                                                                                                                        >  1:1968591/1‑150 (MQ=255)
              cGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATc                                                                                                                                        <  1:2263954/150‑1 (MQ=255)
                tGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCaa                                                                                                                                      >  1:1813838/1‑150 (MQ=255)
                tGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCaa                                                                                                                                      >  1:3546361/1‑150 (MQ=255)
                 gACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAg                                                                                                                                     >  1:2692540/1‑150 (MQ=255)
                    aTACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAgcgc                                                                                                                                  >  1:3079838/1‑150 (MQ=255)
                       cGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGcatc                                                                                                                               <  1:2287422/150‑1 (MQ=255)
                        gCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGcatca                                                                                                                              >  1:2511299/1‑150 (MQ=255)
                          aGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCatcatc                                                                                                                            >  1:3831086/1‑150 (MQ=255)
                           gCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCt                                                                                                                           >  2:1645472/1‑150 (MQ=255)
                            cTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTg                                                                                                                          <  2:3102199/150‑1 (MQ=255)
                             tttttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGc                                                                                                                         <  2:3670778/150‑1 (MQ=255)
                                  cAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCa                                                                                                                    >  1:3426730/1‑150 (MQ=255)
                                   aGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAg                                                                                                                   >  1:2444739/1‑150 (MQ=255)
                                    ggTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGt                                                                                                                  <  1:756571/150‑1 (MQ=255)
                                     gTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTg                                                                                                                 >  2:593433/1‑150 (MQ=255)
                                          tGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGcc                                                                                                            >  2:3361063/1‑150 (MQ=255)
                                            aaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCag                                                                                                          >  1:2905123/1‑150 (MQ=255)
                                            aaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCag                                                                                                          >  1:167885/1‑150 (MQ=255)
                                            aaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCag                                                                                                          >  1:145186/1‑150 (MQ=255)
                                            aaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCag                                                                                                          <  1:983374/150‑1 (MQ=255)
                                             atatTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCaga                                                                                                         >  1:1070842/1‑150 (MQ=255)
                                               atTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAgaga                                                                                                       <  2:2829983/150‑1 (MQ=255)
                                                  tGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaa                                                                                                    >  1:479871/1‑150 (MQ=255)
                                                     aaaaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAc                                                                                                 >  2:4406624/1‑150 (MQ=255)
                                                      aaaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAcc                                                                                                >  1:1621521/1‑150 (MQ=255)
                                                      aaaaGAAGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAAACCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAcc                                                                                                >  2:734072/1‑150 (MQ=255)
                                                        aaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAcccc                                                                                              <  2:540211/150‑1 (MQ=255)
                                                         aGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCg                                                                                             >  2:2332299/1‑150 (MQ=255)
                                                          gACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGt                                                                                            >  2:3981539/1‑150 (MQ=255)
                                                                aaaTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCt                                                                                      >  1:2103529/1‑150 (MQ=255)
                                                                aaaTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCt                                                                                      >  1:868602/1‑150 (MQ=255)
                                                                   tCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGcc                                                                                   <  1:3256508/150‑1 (MQ=255)
                                                                    cccACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCa                                                                                  <  2:248376/150‑1 (MQ=255)
                                                                    cccACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCa                                                                                  <  2:1568343/150‑1 (MQ=255)
                                                                     ccaccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCaa                                                                                 >  1:1301996/1‑150 (MQ=255)
                                                                      caccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAc                                                                                >  1:194512/1‑150 (MQ=255)
                                                                      caccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACACATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAc                                                                                >  1:1225708/1‑150 (MQ=255)
                                                                        ccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCa                                                                              <  2:1159331/150‑1 (MQ=255)
                                                                        ccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCa                                                                              >  1:4112161/1‑150 (MQ=255)
                                                                         caTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAg                                                                             >  2:554518/1‑150 (MQ=255)
                                                                         caTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAg                                                                             <  2:931462/150‑1 (MQ=255)
                                                                          aTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAgg                                                                            <  1:4189326/150‑1 (MQ=255)
                                                                            tGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAt                                                                          <  2:2959760/150‑1 (MQ=255)
                                                                               aaTGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGt                                                                       >  2:1523682/1‑150 (MQ=255)
                                                                                aTGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGtt                                                                      >  2:1220907/1‑150 (MQ=255)
                                                                                  gACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGtttt                                                                    <  1:608016/150‑1 (MQ=255)
                                                                                    cacCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCa                                                                  >  1:2727875/1‑150 (MQ=255)
                                                                                          gCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTg                                                            <  2:670164/150‑1 (MQ=255)
                                                                                            accaccAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCt                                                          <  1:3557417/150‑1 (MQ=255)
                                                                                              caccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCttt                                                        >  2:4055090/1‑150 (MQ=255)
                                                                                              caccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCttt                                                        >  2:1513547/1‑150 (MQ=255)
                                                                                               accaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTa                                                       <  1:3378448/150‑1 (MQ=255)
                                                                                               accaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTa                                                       >  1:455656/1‑150 (MQ=255)
                                                                                                  agcagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGc                                                    >  2:1990860/1‑150 (MQ=255)
                                                                                                  agcagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGc                                                    >  2:2163921/1‑150 (MQ=255)
                                                                                                   gcagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCa                                                   <  2:2878324/150‑1 (MQ=255)
                                                                                                      gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACt                                                <  1:4164544/150‑1 (MQ=255)
                                                                                                      gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACt                                                <  2:610585/150‑1 (MQ=255)
                                                                                                         cgcATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCcg                                             <  1:4466313/150‑1 (MQ=255)
                                                                                                                 ccTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTc                                     >  1:1750292/1‑150 (MQ=255)
                                                                                                                  cTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCt                                    <  2:2631006/150‑1 (MQ=255)
                                                                                                                   tACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                                   <  1:702918/150‑1 (MQ=255)
                                                                                                                   tACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                                   <  1:4063481/150‑1 (MQ=255)
                                                                                                                   tACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCAAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                                   <  2:4275288/150‑1 (MQ=255)
                                                                                                                    aCCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGa                                  >  2:2074422/1‑150 (MQ=255)
                                                                                                                     ccAAATCCATCGCATTACCCATGGGGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAg                                 <  1:651542/150‑1 (MQ=255)
                                                                                                                     ccAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAg                                 <  1:2609974/150‑1 (MQ=255)
                                                                                                                         aTCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCga                             >  2:2419472/1‑150 (MQ=255)
                                                                                                                           ccATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCgaga                           <  2:2267557/150‑1 (MQ=255)
                                                                                                                            cATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGaa                          >  1:2215453/1‑150 (MQ=255)
                                                                                                                             aTCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAc                         >  1:1008753/1‑150 (MQ=255)
                                                                                                                             aTCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGAGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAc                         >  1:3660138/1‑150 (MQ=255)
                                                                                                                              tCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACa                        <  2:3586682/150‑1 (MQ=255)
                                                                                                                              tCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACa                        >  1:1409200/1‑150 (MQ=255)
                                                                                                                               cGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt                       >  2:1699606/1‑150 (MQ=255)
                                                                                                                                gCATTACCCATGGCGGTAATCGGAGACTCGGCCAACAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGACACGCTGCCAACCAGGATGTTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATc                      <  1:4371444/150‑1 (MQ=255)
                                                                                                                                  aTTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCg                    >  1:3196193/1‑150 (MQ=255)
                                                                                                                                  aTTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCg                    <  2:3831086/150‑1 (MQ=255)
                                                                                                                                   ttACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGt                   >  2:3262297/1‑150 (MQ=255)
                                                                                                                                    tACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGtt                  >  2:499855/1‑150 (MQ=255)
                                                                                                                                      cccATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTa                <  1:3930189/150‑1 (MQ=255)
                                                                                                                                         aTGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATg             >  2:4122233/1‑150 (MQ=255)
                                                                                                                                              ggTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCgg        >  2:4207829/1‑150 (MQ=255)
                                                                                                                                              ggTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCgg        >  1:4267350/1‑150 (MQ=255)
                                                                                                                                                tAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTa      >  2:351020/1‑150 (MQ=255)
                                                                                                                                                tAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTa      <  1:1818713/150‑1 (MQ=255)
                                                                                                                                                tAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTa      >  2:351386/1‑150 (MQ=255)
                                                                                                                                                 aaTCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAg     >  1:2458395/1‑150 (MQ=255)
                                                                                                                                                  aTCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGc    >  2:3056758/1‑150 (MQ=255)
                                                                                                                                                   tCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCg   <  1:4023123/150‑1 (MQ=255)
                                                                                                                                                    cGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCgg  >  2:1894426/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  CP009273/1959767‑1960064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑