Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,982 A→T 17.8% S1188S (TCA→TCT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,9820AT17.8% 579.0 / 108.4 212S1188S (TCA→TCT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base A (104/70);  new base T (16/22);  total (120/92)
Fisher's exact test for biased strand distribution p-value = 6.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

TTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCA  >  CP009273/2041833‑2042131
                                                                                                                                                     |                                                                                                                                                     
    tgGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCct                                                                                                                                                   <  1:2057898/150‑1 (MQ=255)
       cGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCtt                                                                                                                                                <  1:4480718/150‑1 (MQ=255)
         gACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCa                                                                                                                                              <  2:1665364/150‑1 (MQ=255)
           cAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAgg                                                                                                                                            <  1:2873575/150‑1 (MQ=255)
            aaaGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGa                                                                                                                                           >  1:492584/1‑150 (MQ=255)
              aGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACt                                                                                                                                         >  1:2740610/1‑150 (MQ=255)
              aGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACt                                                                                                                                         <  2:202663/150‑1 (MQ=255)
                 cTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCAc                                                                                                                                      >  1:1252157/1‑150 (MQ=255)
                   cGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCAccc                                                                                                                                    >  2:3002243/1‑150 (MQ=255)
                    ggCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCt                                                                                                                                   >  2:48907/1‑150 (MQ=255)
                        cAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGAcc                                                                                                                               >  1:3381705/1‑150 (MQ=255)
                          ggTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGAcccc                                                                                                                             <  1:1649040/150‑1 (MQ=255)
                               tCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGag                                                                                                                        <  1:1187056/150‑1 (MQ=255)
                               tCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGag                                                                                                                        <  1:2983173/150‑1 (MQ=255)
                                ccTGCAGATATCAAAAGATGAGATCACAGGTAATGGTGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagt                                                                                                                       >  2:1170405/1‑150 (MQ=255)
                                   gCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaag                                                                                                                    <  2:2818460/150‑1 (MQ=255)
                                    cAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaagt                                                                                                                   <  2:218128/150‑1 (MQ=255)
                                     aGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaagta                                                                                                                  <  2:911599/150‑1 (MQ=255)
                                       atatCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAAt                                                                                                                <  2:3354578/150‑1 (MQ=255)
                                       atatCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAAt                                                                                                                >  1:2775162/1‑150 (MQ=255)
                                       atatCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAAt                                                                                                                <  1:2331161/150‑1 (MQ=255)
                                         atCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATAc                                                                                                              >  1:3810762/1‑150 (MQ=255)
                                           cAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCa                                                                                                            >  2:844588/1‑150 (MQ=255)
                                            aaaaGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCaa                                                                                                           >  2:1752211/1‑150 (MQ=255)
                                              aaGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACg                                                                                                         <  2:4196955/150‑1 (MQ=255)
                                                gATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAg                                                                                                       >  1:4038927/1‑150 (MQ=255)
                                                 aTGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGt                                                                                                      >  1:63323/1‑150 (MQ=255)
                                                    agaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTg                                                                                                   >  2:577683/1‑150 (MQ=255)
                                                    agaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTg                                                                                                   >  2:404734/1‑150 (MQ=255)
                                                     gaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTgg                                                                                                  >  1:1951708/1‑150 (MQ=255)
                                                      aTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGc                                                                                                 <  2:1981426/150‑1 (MQ=255)
                                                      aTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGc                                                                                                 <  2:1979515/150‑1 (MQ=255)
                                                         acaGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATc                                                                                              >  2:4463183/1‑150 (MQ=255)
                                                          caGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATcg                                                                                             >  2:3993112/1‑150 (MQ=255)
                                                           aGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATcgc                                                                                            >  2:1502768/1‑150 (MQ=255)
                                                           aGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATcgc                                                                                            >  2:1342966/1‑150 (MQ=255)
                                                              tAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCa                                                                                         >  2:3487140/1‑150 (MQ=255)
                                                               aaTGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAg                                                                                        >  2:2390076/1‑150 (MQ=255)
                                                               aaTGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAg                                                                                        >  2:2719670/1‑150 (MQ=255)
                                                                aTGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAgg                                                                                       >  2:1539755/1‑150 (MQ=255)
                                                                 tGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGc                                                                                      <  1:3077094/150‑1 (MQ=255)
                                                                  ggCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGcc                                                                                     >  1:1600801/1‑150 (MQ=255)
                                                                       cGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCActct                                                                                <  1:1047130/150‑1 (MQ=255)
                                                                        gATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACtctc                                                                               >  1:494875/1‑150 (MQ=255)
                                                                        gATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACtctc                                                                               <  1:1953910/150‑1 (MQ=255)
                                                                        gATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACtctc                                                                               >  1:2760095/1‑150 (MQ=255)
                                                                        gATAGCGAAACGCTAACTGCACCGGTTAAATATCAGTTCGACATTGAGGTGAACAATCTTCCGGTAACATTCAGCTCAGACTCTACAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACtctc                                                                               <  1:4231558/150‑1 (MQ=255)
                                                                         aTAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCg                                                                              <  1:3676677/150‑1 (MQ=255)
                                                                         aTAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGTCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCg                                                                              >  1:1447278/1‑150 (MQ=255)
                                                                            gcgcAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAg                                                                           <  2:3607376/150‑1 (MQ=255)
                                                                            gcgcAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAg                                                                           <  2:2307152/150‑1 (MQ=255)
                                                                               cAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCg                                                                        >  1:3034736/1‑150 (MQ=255)
                                                                                 aCGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGtt                                                                      <  1:2504801/150‑1 (MQ=255)
                                                                                 aCGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGtt                                                                      <  2:3381705/150‑1 (MQ=255)
                                                                                     tAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCt                                                                  <  2:1380627/150‑1 (MQ=255)
                                                                                      aaCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCtt                                                                 <  2:2623280/150‑1 (MQ=255)
                                                                                      aaCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCtt                                                                 >  2:1902990/1‑150 (MQ=255)
                                                                                       aCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCttt                                                                >  1:1282956/1‑150 (MQ=255)
                                                                                         tGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTgg                                                              >  2:561400/1‑150 (MQ=255)
                                                                                         tGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTgg                                                              >  2:1292347/1‑150 (MQ=255)
                                                                                            aaCGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTga                                                           >  1:4257300/1‑150 (MQ=255)
                                                                                              cGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTgaga                                                         >  1:2916603/1‑150 (MQ=255)
                                                                                              cGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTGTGGTgaga                                                         >  2:3108626/1‑150 (MQ=255)
                                                                                               ggTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaa                                                        <  1:2701360/150‑1 (MQ=255)
                                                                                               ggTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaa                                                        >  1:825086/1‑150 (MQ=255)
                                                                                                 ttAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGagaaga                                                      <  1:2750840/150‑1 (MQ=255)
                                                                                                  tAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGAc                                                     <  1:272627/150‑1 (MQ=255)
                                                                                                     aGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGt                                                  >  2:3727337/1‑150 (MQ=255)
                                                                                                     aGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGt                                                  >  1:3559540/1‑150 (MQ=255)
                                                                                                      gATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGtt                                                 >  2:42744/1‑150 (MQ=255)
                                                                                                      gATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGtt                                                 >  2:42946/1‑150 (MQ=255)
                                                                                                         cAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACt                                              <  2:733015/150‑1 (MQ=255)
                                                                                                          aGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTg                                             <  1:2553102/150‑1 (MQ=255)
                                                                                                           gTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGc                                            >  2:3084508/1‑150 (MQ=255)
                                                                                                           gTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGc                                            >  2:546089/1‑150 (MQ=255)
                                                                                                           gTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAAAACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGc                                            >  2:822361/1‑150 (MQ=255)
                                                                                                             tCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCAt                                          <  2:1846609/150‑1 (MQ=255)
                                                                                                              cGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATc                                         <  2:3267668/150‑1 (MQ=255)
                                                                                                               gACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCa                                        <  2:3813605/150‑1 (MQ=255)
                                                                                                                  aaTGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCTGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTg                                     >  1:1076712/1‑150 (MQ=255)
                                                                                                                     gAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCt                                  >  1:2878273/1‑150 (MQ=255)
                                                                                                                         tGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCtaata                              <  2:2709322/150‑1 (MQ=255)
                                                                                                                            ataatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATg                           <  1:4445493/150‑1 (MQ=255)
                                                                                                                              aatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGt                         <  2:3700509/150‑1 (MQ=255)
                                                                                                                               atCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTg                        >  1:2395643/1‑150 (MQ=255)
                                                                                                                               atCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTg                        >  1:682854/1‑150 (MQ=255)
                                                                                                                               atCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTg                        >  1:2204678/1‑150 (MQ=255)
                                                                                                                                  ttCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCa                     <  2:1047964/150‑1 (MQ=255)
                                                                                                                                    ccGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGc                   >  1:4365348/1‑150 (MQ=255)
                                                                                                                                     cGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCg                  >  1:3871592/1‑150 (MQ=255)
                                                                                                                                       gTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGac                >  1:3645302/1‑150 (MQ=255)
                                                                                                                                       gTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGac                >  1:4494279/1‑150 (MQ=255)
                                                                                                                                       gTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGac                <  1:1539755/150‑1 (MQ=255)
                                                                                                                                        tAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTGATAATGGTGCCAGCGaca               <  2:2308779/150‑1 (MQ=255)
                                                                                                                                        tAACATTCAGCTCAGCCTCTTCAGGACTAACCATGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGATGCCTATGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGaca               >  2:1807637/1‑150 (MQ=255)
                                                                                                                                          aCATTCAGCTCAGCCTCTTCAGTACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGacaac             >  1:2029876/1‑150 (MQ=255)
                                                                                                                                                agctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACt       >  2:2229462/1‑150 (MQ=255)
                                                                                                                                                 gctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACtg      <  2:2589472/150‑1 (MQ=255)
                                                                                                                                                  ctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACtgt     <  1:2492382/150‑1 (MQ=255)
                                                                                                                                                    cagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGc   <  2:1295128/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCA  >  CP009273/2041833‑2042131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑