Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,220,503 C→T 8.9% V83M (GTG→ATG)  yohF ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,220,5030CT 8.9% 862.6 / 52.5 259V83M (GTG→ATG) yohFputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (139/97);  new base T (12/11);  total (151/108)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 271 total aligned reads displayed.

GCCCTGCCCTTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCC  >  CP009273/2220354‑2220652
                                                                                                                                                     |                                                                                                                                                     
gccctgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGcac                                                                                                                                                       >  1:991953/1‑150 (MQ=255)
 ccctgccctTGTTTCACCATCTGACGAGCCGCATTTTGCGAGCATAAGAATGCACCATCGACATCAACGTTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGcaca                                                                                                                                                      <  2:1539562/150‑1 (MQ=255)
 ccctgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGcaca                                                                                                                                                      <  2:4490686/150‑1 (MQ=255)
   ctgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATc                                                                                                                                                    <  2:1346695/150‑1 (MQ=255)
    tgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCa                                                                                                                                                   >  2:4200013/1‑150 (MQ=255)
     gccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCaa                                                                                                                                                  >  1:3827320/1‑150 (MQ=255)
        ctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATgc                                                                                                                                               >  2:3608136/1‑150 (MQ=255)
           gTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGcc                                                                                                                                            >  2:327283/1‑150 (MQ=255)
             ttCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGcccc                                                                                                                                          >  2:1371432/1‑150 (MQ=255)
              tCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCa                                                                                                                                         <  2:279486/150‑1 (MQ=255)
                 ccATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGcc                                                                                                                                      >  2:1407158/1‑150 (MQ=255)
                  cATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCg                                                                                                                                     >  2:1531826/1‑150 (MQ=255)
                     cTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTg                                                                                                                                  >  1:1790057/1‑150 (MQ=255)
                        aCGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAAt                                                                                                                               >  1:1649032/1‑150 (MQ=255)
                         cGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATg                                                                                                                              >  1:1532408/1‑150 (MQ=255)
                           aGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAg                                                                                                                            >  2:2463896/1‑150 (MQ=255)
                           aGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAg                                                                                                                            >  2:569813/1‑150 (MQ=255)
                              cgaattttGCGTGCATAAGATTGCACCTTCGACATCACCGTTAAAAATCTTGCGCCACTCATCAAACGCCTTATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGttt                                                                                                                         <  1:3113413/145‑1 (MQ=255)
                              cGCAATTTGCTAGCATAATAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGttt                                                                                                                         <  1:1029378/150‑1 (MQ=255)
                              cGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGttt                                                                                                                         <  2:398134/150‑1 (MQ=255)
                              cGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGttt                                                                                                                         <  2:3856508/150‑1 (MQ=255)
                                   tttGCGAGCATAAGAATGCACCATCGACATCACCGGTAAAAATCTTGCGCCACTCGTCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCa                                                                                                                    <  2:1575493/150‑1 (MQ=255)
                                     tGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAgc                                                                                                                  >  1:4040730/1‑150 (MQ=255)
                                         aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGACACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCa                                                                                                              <  1:537345/150‑1 (MQ=255)
                                         aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCa                                                                                                              >  2:2927240/1‑150 (MQ=255)
                                         aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCa                                                                                                              >  1:3768121/1‑150 (MQ=255)
                                         aGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAACCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCa                                                                                                              <  1:474165/150‑1 (MQ=255)
                                           cATAAGATTGCCCCTTCGCCATAAAGTGTAAAAATCTGGCGCCACTCATAAAACGCCATATCCTGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGt                                                                                                            <  1:1910736/150‑1 (MQ=255)
                                           cATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGt                                                                                                            >  1:3248064/1‑150 (MQ=255)
                                              aaGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGcc                                                                                                         >  2:914919/1‑150 (MQ=255)
                                              aaGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGcc                                                                                                         <  1:734517/150‑1 (MQ=255)
                                                gAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGcccc                                                                                                       >  2:2178718/1‑150 (MQ=255)
                                                      aCCATCGACATCAACGGTAAAAATCTGGCGCCACTCATCCAAACCCATCTCAAGAAACGGCGCTTTGGTCTTTGCACCCGCATTATTCACCAGCACATCATTGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTgg                                                                                                 <  1:1297038/150‑1 (MQ=255)
                                                       ccATCGACATCAACGGTAAAAATCTTGCGCCACTCAACAAAAGCCATATCAAGACACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGt                                                                                                <  2:2423707/150‑1 (MQ=255)
                                                        cATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGTACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTa                                                                                               >  1:2451245/1‑150 (MQ=255)
                                                         aTCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAg                                                                                              >  1:3488515/1‑150 (MQ=255)
                                                           cGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAt                                                                                            >  1:4393072/1‑150 (MQ=255)
                                                            gACATCCACGGTAAAAAACTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAtt                                                                                           <  1:142423/150‑1 (MQ=255)
                                                            gACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAtt                                                                                           <  1:1842635/150‑1 (MQ=255)
                                                            gACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAtt                                                                                           >  1:4143121/1‑150 (MQ=255)
                                                               ataaaCTGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGACACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGcc                                                                                        <  1:2066945/147‑1 (MQ=255)
                                                                 cAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCga                                                                                      >  2:3485849/1‑150 (MQ=255)
                                                                 cAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCga                                                                                      >  1:40023/1‑150 (MQ=255)
                                                                 cAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCga                                                                                      >  1:4321035/1‑150 (MQ=255)
                                                                  aaCGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCgag                                                                                     <  2:2327323/150‑1 (MQ=255)
                                                                      gTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATcc                                                                                 <  2:1834218/150‑1 (MQ=255)
                                                                       tAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCa                                                                                >  1:2532747/1‑150 (MQ=255)
                                                                        aaaaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAg                                                                               >  1:1251863/1‑150 (MQ=255)
                                                                          aaaTCTTGCGCCACTCATCAAAAGCCATATCAAGACACGGCGCTTTGGTCAGTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCt                                                                             <  2:967491/150‑1 (MQ=255)
                                                                          aaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCt                                                                             >  1:1697418/1‑150 (MQ=255)
                                                                              cTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCAc                                                                         <  1:3201816/150‑1 (MQ=255)
                                                                              cTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAAAGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCAc                                                                         >  2:4062461/1‑150 (MQ=255)
                                                                                tGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGa                                                                       >  1:3528948/1‑150 (MQ=255)
                                                                                  cgcCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtc                                                                     >  2:1011256/1‑150 (MQ=255)
                                                                                   gcCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtct                                                                    >  2:864667/1‑150 (MQ=255)
                                                                                   gcCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtct                                                                    <  2:3149158/150‑1 (MQ=255)
                                                                                     cACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTcc                                                                  <  1:2506629/150‑1 (MQ=255)
                                                                                       cTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGc                                                                >  2:4428378/1‑150 (MQ=255)
                                                                                          atcaAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACg                                                             >  1:2860740/1‑150 (MQ=255)
                                                                                             aaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTAc                                                          >  2:657132/1‑150 (MQ=255)
                                                                                             aaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTAc                                                          >  2:786769/1‑150 (MQ=255)
                                                                                              aaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACg                                                         >  2:1128868/1‑150 (MQ=255)
                                                                                              aaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACg                                                         >  2:4271526/1‑150 (MQ=255)
                                                                                                aGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGcc                                                       >  2:2570852/1‑150 (MQ=255)
                                                                                                 gCCATATCAAGACACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCg                                                      <  1:2717824/150‑1 (MQ=255)
                                                                                                   cATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTg                                                    <  1:2078831/150‑1 (MQ=255)
                                                                                                   cATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTg                                                    <  1:2403291/150‑1 (MQ=255)
                                                                                                    atatCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTgg                                                   >  1:2347096/1‑150 (MQ=255)
                                                                                                    atatCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTgg                                                   >  2:1918457/1‑150 (MQ=255)
                                                                                                      atCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTTCCGCGATCCAGCTGCACGATCTCCCCACGTACGCCGGGGcg                                                 >  1:1142207/1‑149 (MQ=255)
                                                                                                        cAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTaa                                               <  1:2911442/150‑1 (MQ=255)
                                                                                                          aGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACt                                             >  1:704049/1‑150 (MQ=255)
                                                                                                           gacaCGGCGCGTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTa                                            <  1:842244/147‑1 (MQ=255)
                                                                                                           gAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTa                                            >  2:355577/1‑150 (MQ=255)
                                                                                                             aaCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTAcc                                          <  2:494002/150‑1 (MQ=255)
                                                                                                             aaCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTAcc                                          >  2:3065443/1‑150 (MQ=255)
                                                                                                                ggCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCa                                       >  2:3766761/1‑150 (MQ=255)
                                                                                                                  cgcTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCAcg                                     >  2:2249451/1‑150 (MQ=255)
                                                                                                                      ttGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCAcgcgcg                                 <  2:2311698/150‑1 (MQ=255)
                                                                                                                       tGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCgg                                >  2:3331995/1‑150 (MQ=255)
                                                                                                                       tGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCgg                                >  1:802025/1‑150 (MQ=255)
                                                                                                                       tGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCgg                                >  1:800209/1‑150 (MQ=255)
                                                                                                                            aTTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                           <  2:1257446/150‑1 (MQ=255)
                                                                                                                            aTTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                           >  2:2723975/1‑150 (MQ=255)
                                                                                                                               gCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCtttt                        <  1:2220656/150‑1 (MQ=255)
                                                                                                                                   ccGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGccc                    >  1:3944130/1‑150 (MQ=255)
                                                                                                                                         tattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttctt              >  1:615283/1‑150 (MQ=255)
                                                                                                                                         tattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttctt              >  1:2763135/1‑150 (MQ=255)
                                                                                                                                          attCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCttcttc             >  1:1886781/1‑150 (MQ=255)
                                                                                                                                          attCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCttcttc             <  2:3376581/150‑1 (MQ=255)
                                                                                                                                           ttCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCa            <  1:2209437/150‑1 (MQ=255)
                                                                                                                                           ttCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCa            <  1:3746733/150‑1 (MQ=255)
                                                                                                                                             cACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATc          >  1:2389263/1‑150 (MQ=255)
                                                                                                                                             cACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATc          <  2:3778480/150‑1 (MQ=255)
                                                                                                                                             cACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCGTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATc          >  1:123141/1‑150 (MQ=255)
                                                                                                                                               ccAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTg        >  1:3342053/1‑150 (MQ=255)
                                                                                                                                               ccAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTGCTGGGAGATTGCCGAGATCCAGATGCACGATCTCCGCACGTACGCCGTGGCTCACTACCTCACGCGCGGTATCTGTTGCCCCTTCTTCAGCTg        >  2:2862098/1‑150 (MQ=255)
                                                                                                                                                 aGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAg      <  1:3813678/150‑1 (MQ=255)
                                                                                                                                                  gCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGt     <  2:1627503/150‑1 (MQ=255)
                                                                                                                                                    acaTCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGc   >  2:3205538/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCCTGCCCTTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCC  >  CP009273/2220354‑2220652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑