Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,374 C→A 83.8% V142V (GTG→GTT)  ccmC ← heme exporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,3740CA83.8% 639.8 / 132.6 266V142V (GTG→GTT) ccmCheme exporter subunit
Reads supporting (aligned to +/- strand):  ref base C (16/27);  new base A (104/119);  total (120/146)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 272 total aligned reads displayed.

CTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCGC  >  CP009273/2289227‑2289520
                                                                                                                                                   |                                                                                                                                                  
ctgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATaac                                                                                                                                                  >  1:3645363/1‑150 (MQ=255)
 tgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATcaca                                                                                                                                                 >  1:1110967/1‑150 (MQ=255)
 tgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATcaca                                                                                                                                                 >  1:3396739/1‑150 (MQ=255)
   cATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAcc                                                                                                                                               <  2:2901985/150‑1 (MQ=255)
   cATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAcc                                                                                                                                               >  1:67040/1‑150 (MQ=255)
    aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACAccc                                                                                                                                              <  1:2534851/150‑1 (MQ=255)
       cgcgTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCcaca                                                                                                                                           >  2:75798/1‑150 (MQ=255)
         cgTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATa                                                                                                                                         <  1:920858/150‑1 (MQ=255)
            tGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaa                                                                                                                                      >  1:2066421/1‑150 (MQ=255)
             gATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACaaa                                                                                                                                     >  1:588786/1‑150 (MQ=255)
             gATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACaaa                                                                                                                                     >  1:3736635/1‑150 (MQ=255)
              aTCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaaa                                                                                                                                    >  1:455551/1‑150 (MQ=255)
              aTCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaaa                                                                                                                                    <  2:1656566/150‑1 (MQ=255)
                cccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAc                                                                                                                                  >  2:3373878/1‑150 (MQ=255)
                 ccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAca                                                                                                                                 <  1:713489/150‑1 (MQ=255)
                  cTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcag                                                                                                                                >  2:2254003/1‑150 (MQ=255)
                    gATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcagca                                                                                                                              <  1:809135/150‑1 (MQ=255)
                     aTGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcagcag                                                                                                                             <  1:3613051/150‑1 (MQ=255)
                       gCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAgcagca                                                                                                                           <  2:1885011/150‑1 (MQ=255)
                        cAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCAc                                                                                                                          <  2:362114/150‑1 (MQ=255)
                          gggTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCa                                                                                                                        <  2:3750843/150‑1 (MQ=255)
                          gggTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCa                                                                                                                        <  2:1386624/150‑1 (MQ=255)
                           ggTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAg                                                                                                                       <  1:1205387/150‑1 (MQ=255)
                               ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCa                                                                                                                   <  2:636453/150‑1 (MQ=255)
                                  caccacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGAAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGaa                                                                                                                <  1:22602/150‑1 (MQ=255)
                                   accacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAg                                                                                                               <  1:1023989/150‑1 (MQ=255)
                                    ccacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                              <  2:1222552/150‑1 (MQ=255)
                                      acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCa                                                                                                            <  2:2329211/150‑1 (MQ=255)
                                      acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCa                                                                                                            >  2:1748438/1‑150 (MQ=255)
                                         ccACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGAc                                                                                                         <  1:1643606/150‑1 (MQ=255)
                                         ccACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGAc                                                                                                         >  1:2771094/1‑150 (MQ=255)
                                          cACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACg                                                                                                        <  1:2300548/150‑1 (MQ=255)
                                          cACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACg                                                                                                        >  2:2747166/1‑150 (MQ=255)
                                          cACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACg                                                                                                        >  2:2612537/1‑150 (MQ=255)
                                             ggAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGc                                                                                                     <  2:1137462/150‑1 (MQ=255)
                                              gAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCa                                                                                                    <  2:2128999/150‑1 (MQ=255)
                                              gAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCa                                                                                                    <  2:4403651/150‑1 (MQ=255)
                                               aGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCAt                                                                                                   >  2:4007258/1‑150 (MQ=255)
                                                gTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTACCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATc                                                                                                  <  2:683257/150‑1 (MQ=255)
                                                   aTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCa                                                                                               <  1:1900343/150‑1 (MQ=255)
                                                    tGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCAt                                                                                              <  2:3736635/150‑1 (MQ=255)
                                                      aataatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATAc                                                                                            >  2:2210522/1‑150 (MQ=255)
                                                            cGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccacca                                                                                      >  1:1732257/1‑150 (MQ=255)
                                                             ggCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAg                                                                                     >  1:2456487/1‑150 (MQ=255)
                                                               cAGATTCACCACGCCAATCAGCACCAGGATTCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGt                                                                                   <  2:2231448/150‑1 (MQ=255)
                                                                aGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTg                                                                                  <  1:4316728/150‑1 (MQ=255)
                                                                  aTTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGcc                                                                                >  1:2593717/1‑150 (MQ=255)
                                                                  aTTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGcc                                                                                >  1:2593725/1‑150 (MQ=255)
                                                                     caccacGCCAATCAGCACCAGGAGACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACCGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCca                                                                             <  2:915643/150‑1 (MQ=255)
                                                                          cGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCg                                                                        <  1:3180933/150‑1 (MQ=255)
                                                                          cGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCAAACCCACCAGGTGCCCCACATCg                                                                        >  2:2981151/1‑150 (MQ=255)
                                                                             cAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGtt                                                                     >  2:2591790/1‑150 (MQ=255)
                                                                              aaTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGttt                                                                    >  2:822513/1‑150 (MQ=255)
                                                                              aaTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGttt                                                                    <  2:3318912/150‑1 (MQ=255)
                                                                               aTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGtttt                                                                   <  1:400634/150‑1 (MQ=255)
                                                                                tCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTc                                                                  >  1:2869373/1‑150 (MQ=255)
                                                                                   gCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTcccc                                                               <  1:4125826/150‑1 (MQ=255)
                                                                                    cACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCa                                                              >  1:1556818/1‑150 (MQ=255)
                                                                                    cACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCa                                                              >  1:3597688/1‑150 (MQ=255)
                                                                                    cACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCa                                                              <  1:1283078/150‑1 (MQ=255)
                                                                                     aCCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAt                                                             <  2:1569818/150‑1 (MQ=255)
                                                                                       cAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGc                                                           <  2:3853806/150‑1 (MQ=255)
                                                                                        aGGATTCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCa                                                          <  1:1188871/150‑1 (MQ=255)
                                                                                        aGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCa                                                          <  1:3473796/150‑1 (MQ=255)
                                                                                        aGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCa                                                          >  2:1179769/1‑150 (MQ=255)
                                                                                           aTACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                       <  2:4364435/150‑1 (MQ=255)
                                                                                           aTACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                       <  2:3190977/150‑1 (MQ=255)
                                                                                           aTACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCagag                                                       <  2:1043978/150‑1 (MQ=255)
                                                                                            tACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGc                                                      <  2:1847446/150‑1 (MQ=255)
                                                                                             aCCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGcc                                                     <  1:1428081/150‑1 (MQ=255)
                                                                                              ccTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCg                                                    <  2:1910679/150‑1 (MQ=255)
                                                                                                tGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGt                                                  <  1:1234822/150‑1 (MQ=255)
                                                                                                 gCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTa                                                 <  2:3407270/150‑1 (MQ=255)
                                                                                                      aCGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAg                                            <  2:2771094/150‑1 (MQ=255)
                                                                                                        ggCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAggg                                          <  2:1012268/150‑1 (MQ=255)
                                                                                                        ggCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAggg                                          <  2:1070443/150‑1 (MQ=255)
                                                                                                          cccGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCa                                        >  1:3833383/1‑150 (MQ=255)
                                                                                                          cccGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCa                                        <  1:3558330/150‑1 (MQ=255)
                                                                                                           ccGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCaa                                       >  1:2888183/1‑150 (MQ=255)
                                                                                                            cGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAAt                                      <  2:3906099/150‑1 (MQ=255)
                                                                                                                    ggcggTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTaa                              <  1:3277667/150‑1 (MQ=255)
                                                                                                                         tcgtcgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACg                         <  2:2998987/150‑1 (MQ=255)
                                                                                                                           gtcgAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGc                       >  2:3939185/1‑150 (MQ=255)
                                                                                                                           gtcgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGc                       >  1:3021859/1‑150 (MQ=255)
                                                                                                                            tcgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCa                      <  2:2532634/150‑1 (MQ=255)
                                                                                                                              gAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAcc                    >  2:2552478/1‑150 (MQ=255)
                                                                                                                              gAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAcc                    >  1:1437937/1‑150 (MQ=255)
                                                                                                                                 ggCGTGCCACAGGGAAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAAt                 <  1:4411599/150‑1 (MQ=255)
                                                                                                                                  gCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATg                <  1:2531983/150‑1 (MQ=255)
                                                                                                                                  gCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATg                <  2:792221/150‑1 (MQ=255)
                                                                                                                                   cGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCCGGGCACTAAAGGTAAACACGGCACCAATgg               >  2:2278277/1‑150 (MQ=255)
                                                                                                                                   cGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATgg               >  2:2928494/1‑150 (MQ=255)
                                                                                                                                   cGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATgg               >  1:4103149/1‑150 (MQ=255)
                                                                                                                                   cGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATgg               >  1:1825485/1‑150 (MQ=255)
                                                                                                                                    gTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATggg              >  1:3032864/1‑150 (MQ=255)
                                                                                                                                         acaGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCa         <  2:2676479/150‑1 (MQ=255)
                                                                                                                                         acaGGGCAATAACCCCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCCGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCa         <  1:1185357/150‑1 (MQ=255)
                                                                                                                                         acaGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCa         >  2:1435239/1‑150 (MQ=255)
                                                                                                                                             ggCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATcgc     <  2:4153634/150‑1 (MQ=255)
                                                                                                                                               cAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATcgccg   <  1:2279409/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCGC  >  CP009273/2289227‑2289520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑