Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,353,889 C→A 19.2% V335V (GTG→GTT)  rhmD ← L‑rhamnonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,353,8890CA19.2% 716.1 / 122.2 256V335V (GTG→GTT) rhmDL‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (116/90);  new base A (26/24);  total (142/114)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

AAACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGC  >  CP009273/2353740‑2354036
                                                                                                                                                     |                                                                                                                                                   
aaaCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATc                                                                                                                                                     <  2:3191371/150‑1 (MQ=255)
  aCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAAc                                                                                                                                                   <  2:2043251/150‑1 (MQ=255)
    cGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCg                                                                                                                                                 >  1:861200/1‑150 (MQ=255)
       gTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCAt                                                                                                                                              >  2:3278118/1‑150 (MQ=255)
       gTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCAt                                                                                                                                              >  2:3023606/1‑150 (MQ=255)
         ttATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCCTgg                                                                                                                                            >  1:1359536/1‑150 (MQ=255)
          tATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGt                                                                                                                                           >  2:1180873/1‑150 (MQ=255)
          tATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGt                                                                                                                                           <  2:2189706/150‑1 (MQ=255)
             cAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTgag                                                                                                                                        >  2:2119651/1‑150 (MQ=255)
               aGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGagag                                                                                                                                      <  1:3122171/150‑1 (MQ=255)
                gCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGt                                                                                                                                     <  2:1823291/150‑1 (MQ=255)
                 cACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTa                                                                                                                                    >  2:3887857/1‑150 (MQ=255)
                   cTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTaaa                                                                                                                                  >  1:3759506/1‑150 (MQ=255)
                    tGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTCTTGGTGAAGGTGATAACCGCATGGTGAGAGTAAAc                                                                                                                                 >  2:2609103/1‑150 (MQ=255)
                     gATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACa                                                                                                                                >  1:3941746/1‑150 (MQ=255)
                      aTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAg                                                                                                                               >  2:996677/1‑150 (MQ=255)
                      aTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAg                                                                                                                               <  1:3210722/150‑1 (MQ=255)
                        ttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGAc                                                                                                                             >  1:498497/1‑150 (MQ=255)
                           tGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGaa                                                                                                                          >  2:108109/1‑150 (MQ=255)
                           tGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGaa                                                                                                                          >  2:2307753/1‑150 (MQ=255)
                            gAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAAc                                                                                                                         >  2:3337529/1‑150 (MQ=255)
                             aaTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAAcc                                                                                                                        <  2:2658012/150‑1 (MQ=255)
                              aTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCg                                                                                                                       >  2:3992148/1‑150 (MQ=255)
                                  gACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCg                                                                                                                   <  2:492661/150‑1 (MQ=255)
                                   aCCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCgg                                                                                                                  >  1:2935924/1‑150 (MQ=255)
                                   aCCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCgg                                                                                                                  <  2:921429/150‑1 (MQ=255)
                                      aTTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCAGGGTGAGAGTAAACAGACGAACCGTGCGGcac                                                                                                               >  2:1010311/1‑150 (MQ=255)
                                        tCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGcacca                                                                                                             >  1:3033704/1‑150 (MQ=255)
                                         cACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGcaccac                                                                                                            >  2:705132/1‑150 (MQ=255)
                                           ccGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCAccacca                                                                                                          <  2:4478001/150‑1 (MQ=255)
                                           ccGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCAccacca                                                                                                          <  1:2591273/150‑1 (MQ=255)
                                              gAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGtt                                                                                                       <  2:1377601/150‑1 (MQ=255)
                                              gAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGtt                                                                                                       <  1:736883/150‑1 (MQ=255)
                                               aaCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTg                                                                                                      <  1:341298/150‑1 (MQ=255)
                                               aaCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTg                                                                                                      >  2:2576318/1‑150 (MQ=255)
                                               aaCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTg                                                                                                      >  2:310620/1‑150 (MQ=255)
                                               aaCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTg                                                                                                      <  2:3960899/150‑1 (MQ=255)
                                                     cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                <  1:237318/150‑1 (MQ=255)
                                                     cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                <  2:283346/150‑1 (MQ=255)
                                                      tCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTg                                                                                               >  2:1460563/1‑150 (MQ=255)
                                                       cATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTgg                                                                                              <  2:1330261/150‑1 (MQ=255)
                                                       cATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTgg                                                                                              >  2:427441/1‑150 (MQ=255)
                                                        aTTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGACCCGTGCGGCACCACCAGTTGCCCCCTGGa                                                                                             >  2:1237909/1‑150 (MQ=255)
                                                         ttGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGAt                                                                                            <  1:1152098/150‑1 (MQ=255)
                                                          tGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGAtt                                                                                           >  1:3810536/1‑150 (MQ=255)
                                                          tGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGAtt                                                                                           <  1:4012347/150‑1 (MQ=255)
                                                            aGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTg                                                                                         >  2:1978802/1‑150 (MQ=255)
                                                                 aaTCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGAt                                                                                    >  1:642606/1‑150 (MQ=255)
                                                                  ctCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGAtt                                                                                   <  1:4306491/149‑1 (MQ=255)
                                                                    cGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGc                                                                                 >  1:2247919/1‑150 (MQ=255)
                                                                     gggTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCg                                                                                >  2:637956/1‑150 (MQ=255)
                                                                      ggTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCgg                                                                               >  2:3335655/1‑150 (MQ=255)
                                                                       gTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGc                                                                              <  2:3907847/150‑1 (MQ=255)
                                                                        tCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCa                                                                             <  2:2862600/150‑1 (MQ=255)
                                                                         cAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCaa                                                                            >  2:2025655/1‑150 (MQ=255)
                                                                         cAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCaa                                                                            >  2:1698663/1‑150 (MQ=255)
                                                                            aCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAttt                                                                         <  1:2573036/150‑1 (MQ=255)
                                                                                 ggACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacc                                                                    >  2:2383182/1‑150 (MQ=255)
                                                                                  gACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacca                                                                   >  1:3210676/1‑150 (MQ=255)
                                                                                     gCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCg                                                                >  2:3880173/1‑150 (MQ=255)
                                                                                      cATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTACCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGt                                                               >  1:4445272/1‑150 (MQ=255)
                                                                                       aTCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTg                                                              <  2:1698018/150‑1 (MQ=255)
                                                                                       aTCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTg                                                              >  2:4111760/1‑150 (MQ=255)
                                                                                        tCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTgg                                                             <  1:587778/150‑1 (MQ=255)
                                                                                          gTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCTGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGtt                                                           <  1:357876/150‑1 (MQ=255)
                                                                                          gTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGtt                                                           >  1:3028344/1‑150 (MQ=255)
                                                                                          gTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGtt                                                           >  1:2610206/1‑150 (MQ=255)
                                                                                             gAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCACTTTCCACCAGCGTGGTTaaa                                                        >  2:2703030/1‑150 (MQ=255)
                                                                                                cAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAacca                                                     <  1:768543/150‑1 (MQ=255)
                                                                                                cAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAacca                                                     >  2:1310348/1‑150 (MQ=255)
                                                                                                  aTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccacc                                                   >  2:2167084/1‑150 (MQ=255)
                                                                                                     cGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCa                                                <  2:1871947/150‑1 (MQ=255)
                                                                                                         cTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAg                                            <  1:2395276/150‑1 (MQ=255)
                                                                                                                tcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACAt                                     >  2:3124704/1‑150 (MQ=255)
                                                                                                                 caGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATc                                    >  1:1580253/1‑150 (MQ=255)
                                                                                                                   ggAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCg                                  <  1:2307753/150‑1 (MQ=255)
                                                                                                                        tCGCTGAATGGCGTCTTGGTGAAGGTGATCACCGCATGGGGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGAGTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGc                             >  2:4395733/1‑150 (MQ=255)
                                                                                                                           cTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATa                          <  2:3640887/150‑1 (MQ=255)
                                                                                                                            tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATaa                         >  2:3473479/1‑150 (MQ=255)
                                                                                                                              aaTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATg                       <  1:2533420/150‑1 (MQ=255)
                                                                                                                              aaTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATg                       <  2:373630/150‑1 (MQ=255)
                                                                                                                                tGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                     >  1:3613786/1‑150 (MQ=255)
                                                                                                                                tGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                     <  1:1817622/150‑1 (MQ=255)
                                                                                                                                   cGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGAt                  <  1:2888588/150‑1 (MQ=255)
                                                                                                                                   cGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGAt                  <  1:1983468/150‑1 (MQ=255)
                                                                                                                                   cGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGAt                  >  2:2729285/1‑150 (MQ=255)
                                                                                                                                     tATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAc                >  1:112272/1‑150 (MQ=255)
                                                                                                                                     tATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAc                >  1:1752039/1‑150 (MQ=255)
                                                                                                                                      aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAcc               <  2:2935924/150‑1 (MQ=255)
                                                                                                                                      aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAcc               <  1:2692433/150‑1 (MQ=255)
                                                                                                                                      aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAcc               <  1:427441/150‑1 (MQ=255)
                                                                                                                                        tGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCgg             <  1:4078233/150‑1 (MQ=255)
                                                                                                                                            gAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTCAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTc         >  1:1844878/1‑150 (MQ=255)
                                                                                                                                            gAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTc         >  1:1844511/1‑150 (MQ=255)
                                                                                                                                            gAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTc         >  1:660910/1‑150 (MQ=255)
                                                                                                                                              aGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTg       <  2:551214/150‑1 (MQ=255)
                                                                                                                                              aGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTg       <  2:1415211/150‑1 (MQ=255)
                                                                                                                                               ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGa      >  1:143438/1‑150 (MQ=255)
                                                                                                                                                  gATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAg   >  1:4228868/1‑150 (MQ=255)
                                                                                                                                                   aTCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGc  <  1:2120529/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGC  >  CP009273/2353740‑2354036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑