Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,444,315 C→A 17.4% E80D (GAG→GAT)  yfcQ ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,444,3150CA17.4% 852.7 / 126.0 290E80D (GAG→GAT) yfcQputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (116/123);  new base A (27/24);  total (143/147)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

GCGTAAAATCAGTGGCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGA  >  CP009273/2444167‑2444464
                                                                                                                                                    |                                                                                                                                                     
    aaaaTCAGTGGCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCa                                                                                                                                                  >  2:3918997/1‑150 (MQ=255)
    aaaaTCAGTGGCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCa                                                                                                                                                  >  2:1441188/1‑150 (MQ=255)
    aaaaTCAGTGGCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCa                                                                                                                                                  <  1:3736862/150‑1 (MQ=255)
       aTCAGTGGCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATc                                                                                                                                               <  2:3855090/150‑1 (MQ=255)
            tGGCGTTGATAGCACGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAACCCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGAt                                                                                                                                          <  1:2783030/150‑1 (MQ=255)
             ggCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATg                                                                                                                                         <  2:1366679/150‑1 (MQ=255)
                    aTAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGgtc                                                                                                                                  <  2:2355787/150‑1 (MQ=255)
                    aTAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGgtc                                                                                                                                  <  2:2141915/150‑1 (MQ=255)
                       gCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGgtcgtc                                                                                                                               >  2:2315874/1‑150 (MQ=255)
                         aaGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGa                                                                                                                             >  2:703351/1‑150 (MQ=255)
                              gAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTcac                                                                                                                        >  2:1540913/1‑150 (MQ=255)
                               aGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTcaca                                                                                                                       >  1:4359002/1‑150 (MQ=255)
                               aGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTcaca                                                                                                                       <  2:4240859/150‑1 (MQ=255)
                               aGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTcaca                                                                                                                       >  1:1631465/1‑150 (MQ=255)
                                 tATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAg                                                                                                                     <  1:1494085/150‑1 (MQ=255)
                                  aTTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGt                                                                                                                    <  2:3393400/150‑1 (MQ=255)
                                    tGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGa                                                                                                                  <  2:419068/150‑1 (MQ=255)
                                       gTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGtt                                                                                                               <  1:4059670/150‑1 (MQ=255)
                                       gTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGtt                                                                                                               <  1:4399900/150‑1 (MQ=255)
                                        tttAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTc                                                                                                              <  1:3877287/150‑1 (MQ=255)
                                          tAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCat                                                                                                            <  1:469156/150‑1 (MQ=255)
                                          tAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCat                                                                                                            <  1:2620835/150‑1 (MQ=255)
                                             aaCGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATg                                                                                                         <  1:2213602/150‑1 (MQ=255)
                                                  tGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCAcc                                                                                                    <  2:3603746/150‑1 (MQ=255)
                                                     ccATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACctct                                                                                                 >  2:3873980/1‑150 (MQ=255)
                                                      cATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCtt                                                                                                >  1:316851/1‑150 (MQ=255)
                                                      cATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCtt                                                                                                <  2:4411297/150‑1 (MQ=255)
                                                      cATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCtt                                                                                                <  2:3778450/150‑1 (MQ=255)
                                                       aTCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCttt                                                                                               >  2:2021216/1‑150 (MQ=255)
                                                          gTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACg                                                                                            <  1:2528760/150‑1 (MQ=255)
                                                          gTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACg                                                                                            >  1:3150514/1‑150 (MQ=255)
                                                          gTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACg                                                                                            >  1:2278468/1‑150 (MQ=255)
                                                           tGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTGTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCAGATGGCACCTCTTTACGa                                                                                           >  2:1639272/1‑150 (MQ=255)
                                                            gCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGAc                                                                                          <  1:3915389/150‑1 (MQ=255)
                                                              tGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATCTGGCACCTCTTTACGACCa                                                                                        >  2:1839205/1‑150 (MQ=255)
                                                              tGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCa                                                                                        >  2:2696884/1‑150 (MQ=255)
                                                              tGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCa                                                                                        >  2:587835/1‑150 (MQ=255)
                                                              tGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCa                                                                                        <  2:3311993/150‑1 (MQ=255)
                                                                aaGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATa                                                                                      >  2:866132/1‑150 (MQ=255)
                                                                aaGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATa                                                                                      <  1:2798265/150‑1 (MQ=255)
                                                                 aGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAg                                                                                     >  2:3413121/1‑150 (MQ=255)
                                                                   cTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGtt                                                                                   >  1:796181/1‑150 (MQ=255)
                                                                      gATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGcc                                                                                >  1:2047545/1‑150 (MQ=255)
                                                                       aTACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCg                                                                               >  2:1704963/1‑150 (MQ=255)
                                                                          ccAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTg                                                                            <  1:2510330/150‑1 (MQ=255)
                                                                           cAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGa                                                                           >  2:1486287/1‑150 (MQ=255)
                                                                             aaaCCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATg                                                                         >  1:1029904/1‑150 (MQ=255)
                                                                             aaaCCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATg                                                                         <  2:2643139/150‑1 (MQ=255)
                                                                              aaCCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATgt                                                                        <  1:71884/150‑1 (MQ=255)
                                                                              aaCCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATgt                                                                        <  2:1575843/150‑1 (MQ=255)
                                                                              aaCCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATgt                                                                        >  2:1623583/1‑150 (MQ=255)
                                                                                 cGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATgtcgg                                                                     >  1:1314868/1‑149 (MQ=255)
                                                                                     tACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGAt                                                                 >  2:4479477/1‑150 (MQ=255)
                                                                                     tACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGACTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGAt                                                                 >  2:2822221/1‑150 (MQ=255)
                                                                                      aCATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATa                                                                <  2:4033517/150‑1 (MQ=255)
                                                                                        aTCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAAt                                                              <  2:2974597/150‑1 (MQ=255)
                                                                                         tCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATg                                                             >  2:4360575/1‑150 (MQ=255)
                                                                                            gTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATgaga                                                          <  1:2452459/150‑1 (MQ=255)
                                                                                            gTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATgaga                                                          >  1:135422/1‑150 (MQ=255)
                                                                                             tttCTATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGAt                                                         <  2:2904372/150‑1 (MQ=255)
                                                                                             tttCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGAt                                                         <  2:316851/150‑1 (MQ=255)
                                                                                               tCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATcg                                                       <  1:2952980/150‑1 (MQ=255)
                                                                                                 aaTTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATcgcg                                                     <  2:3939840/150‑1 (MQ=255)
                                                                                                    tgctgcATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGaaa                                                  >  2:531787/1‑150 (MQ=255)
                                                                                                       tgcATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTc                                               <  1:3404941/150‑1 (MQ=255)
                                                                                                         catcatTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGa                                             >  2:3667771/1‑150 (MQ=255)
                                                                                                            catTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACtt                                          <  2:2074363/150‑1 (MQ=255)
                                                                                                                 aaaCTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATg                                     <  1:3780362/150‑1 (MQ=255)
                                                                                                                  aaCTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATgg                                    >  2:4157879/1‑150 (MQ=255)
                                                                                                                   aCTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGt                                   >  2:3324283/1‑150 (MQ=255)
                                                                                                                   aCTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGt                                   <  2:116471/150‑1 (MQ=255)
                                                                                                                   aCTGGTTTGTGTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGt                                   <  2:2384138/150‑1 (MQ=255)
                                                                                                                     tGGTTTGTGTACCAGTCCCGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTAGTCGATAATGAGATCGCGAAATTCGACTTCAATGGttt                                 >  2:3991701/1‑150 (MQ=255)
                                                                                                                     tGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGttt                                 >  2:3571078/1‑150 (MQ=255)
                                                                                                                     tGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGttt                                 <  1:1639272/150‑1 (MQ=255)
                                                                                                                       gTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTa                               <  2:249302/150‑1 (MQ=255)
                                                                                                                          tgtgTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTacca                            >  1:3853567/1‑150 (MQ=255)
                                                                                                                          tgtgTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTacca                            >  1:2826856/1‑150 (MQ=255)
                                                                                                                          tgtgTACCAGTCCAGGTGAGGGTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTacca                            >  1:3194092/1‑150 (MQ=255)
                                                                                                                           gtgtACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTaccac                           <  1:4236855/150‑1 (MQ=255)
                                                                                                                           gtgtACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTaccac                           >  1:3004863/1‑150 (MQ=255)
                                                                                                                            tgtACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTaccacc                          >  1:3282596/1‑150 (MQ=255)
                                                                                                                              tACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGc                        >  2:1745037/1‑150 (MQ=255)
                                                                                                                               aCCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCt                       <  2:635144/150‑1 (MQ=255)
                                                                                                                                ccAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTg                      >  1:3033405/1‑150 (MQ=255)
                                                                                                                                 cAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGa                     <  2:2276346/150‑1 (MQ=255)
                                                                                                                                   gTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATg                   >  2:4098160/1‑150 (MQ=255)
                                                                                                                                    tCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGc                  <  1:1724377/150‑1 (MQ=255)
                                                                                                                                     ccAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCt                 <  2:444289/150‑1 (MQ=255)
                                                                                                                                      cAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTa                <  2:1904605/150‑1 (MQ=255)
                                                                                                                                        ggTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACa              <  1:1441188/150‑1 (MQ=255)
                                                                                                                                             gggTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTg         <  1:949383/150‑1 (MQ=255)
                                                                                                                                             gggTCATATCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTg         <  1:4118804/150‑1 (MQ=255)
                                                                                                                                              ggTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTgg        >  2:2916815/1‑150 (MQ=255)
                                                                                                                                               gTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGc       <  2:4271530/150‑1 (MQ=255)
                                                                                                                                                tCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCg      <  2:3536451/150‑1 (MQ=255)
                                                                                                                                                 cATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCgg     >  2:454618/1‑150 (MQ=255)
                                                                                                                                                 cATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCgg     >  1:1102084/1‑150 (MQ=255)
                                                                                                                                                  aTCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGt    >  1:2874123/1‑150 (MQ=255)
                                                                                                                                                   tctcCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGCCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTg   <  1:1154249/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGTAAAATCAGTGGCGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGGTCGTCGAGTCACAAGTGAGTTCATATGGCACCTCTTTACGACCATAGTTGCCGTTGATGTCGTCGATAATGAGATCGCGAAATTCGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGA  >  CP009273/2444167‑2444464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑