Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,454,297 T→G 88.2% E39D (GAA→GAC)  yfcZ ← UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,454,2970TG88.2% 926.8 / 70.3 266E39D (GAA→GAC) yfcZUPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base T (15/17);  new base G (108/126);  total (123/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAG  >  CP009273/2454148‑2454442
                                                                                                                                                     |                                                                                                                                                 
acaaTCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCGGGCGCACACCGTCAGACGCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCt                                                                                                                                                   <  2:1155594/148‑1 (MQ=255)
 aaaTCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCtt                                                                                                                                                  <  2:1989664/150‑1 (MQ=255)
    tCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTg                                                                                                                                               <  2:781932/150‑1 (MQ=255)
    tCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGAGTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTg                                                                                                                                               <  2:4065169/150‑1 (MQ=255)
     cAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTgc                                                                                                                                              >  1:3043433/1‑150 (MQ=255)
       gCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTgcgc                                                                                                                                            >  2:3215055/1‑150 (MQ=255)
            tCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGttt                                                                                                                                       <  1:1101292/150‑1 (MQ=255)
             cAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTg                                                                                                                                      >  1:376904/1‑150 (MQ=255)
              aGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGc                                                                                                                                     >  2:1602348/1‑150 (MQ=255)
               gCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCa                                                                                                                                    >  2:285288/1‑150 (MQ=255)
               gCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCa                                                                                                                                    >  1:653230/1‑150 (MQ=255)
               gCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCa                                                                                                                                    >  1:694830/1‑150 (MQ=255)
                cTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCaa                                                                                                                                   >  1:4000837/1‑150 (MQ=255)
                   cGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCCGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAaca                                                                                                                                >  2:3891284/1‑150 (MQ=255)
                      aGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACAcgc                                                                                                                             >  2:4516120/1‑150 (MQ=255)
                      aGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgc                                                                                                                             >  1:1484035/1‑150 (MQ=255)
                           aaaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTg                                                                                                                        <  1:138759/150‑1 (MQ=255)
                             aGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTa                                                                                                                      >  2:237294/1‑150 (MQ=255)
                              gTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTaa                                                                                                                     <  1:1299165/150‑1 (MQ=255)
                                gAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGa                                                                                                                   >  1:260055/1‑150 (MQ=255)
                                 aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAc                                                                                                                  <  1:2151872/150‑1 (MQ=255)
                                    tCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAcgcg                                                                                                               >  1:2060812/1‑150 (MQ=255)
                                    tCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAcgcg                                                                                                               >  1:171412/1‑150 (MQ=255)
                                         atCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCa                                                                                                          >  2:607854/1‑150 (MQ=255)
                                         atCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCa                                                                                                          <  2:2716149/150‑1 (MQ=255)
                                         atCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCa                                                                                                          <  1:2470236/150‑1 (MQ=255)
                                         atCCAGGCGCACACCGTCAGAATCTTCAGTGATAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCa                                                                                                          <  1:2982056/150‑1 (MQ=255)
                                             aGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                      >  1:219111/1‑150 (MQ=255)
                                               gcgcACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCCGTCTGCGCCGCCAGCGTGTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGa                                                                                                    <  1:677723/150‑1 (MQ=255)
                                                   acacCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTg                                                                                                >  1:2718944/1‑150 (MQ=255)
                                                   acacCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTg                                                                                                >  1:798765/1‑150 (MQ=255)
                                                    cacCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGt                                                                                               <  2:1291110/150‑1 (MQ=255)
                                                     acCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTc                                                                                              <  1:2728959/150‑1 (MQ=255)
                                                         tCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATa                                                                                          <  2:2760718/150‑1 (MQ=255)
                                                         tCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATa                                                                                          >  2:3577670/1‑150 (MQ=255)
                                                          cAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATaa                                                                                         <  1:3390010/150‑1 (MQ=255)
                                                           aGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAt                                                                                        >  2:1259227/1‑150 (MQ=255)
                                                                 ttCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGcc                                                                                  <  1:2808672/150‑1 (MQ=255)
                                                                  tCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCa                                                                                 >  2:2584157/1‑150 (MQ=255)
                                                                   cAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCaa                                                                                >  2:3138488/1‑150 (MQ=255)
                                                                   cAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCaa                                                                                >  2:3136810/1‑150 (MQ=255)
                                                                      tGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACAt                                                                             <  1:3671056/150‑1 (MQ=255)
                                                                        aaaGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATcc                                                                           <  1:2272869/150‑1 (MQ=255)
                                                                        aaaGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATcc                                                                           >  2:4209740/1‑150 (MQ=255)
                                                                         aaGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCa                                                                          <  1:3842589/150‑1 (MQ=255)
                                                                          aGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAt                                                                         <  1:3116211/150‑1 (MQ=255)
                                                                          aGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAt                                                                         >  1:1243905/1‑150 (MQ=255)
                                                                          aGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAt                                                                         >  2:1228998/1‑150 (MQ=255)
                                                                           gTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATa                                                                        <  1:247462/150‑1 (MQ=255)
                                                                             cGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAca                                                                      <  1:1028630/150‑1 (MQ=255)
                                                                             cGGGGTGATTTTGCAAGGTTCAGAGTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAca                                                                      <  1:543159/150‑1 (MQ=255)
                                                                              ggggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcag                                                                     <  2:3973572/150‑1 (MQ=255)
                                                                              ggggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGACAGCGTTTTATCTGCGTCTGCGAGGTTTGAAAACTCGCGGCTGTAAGACGCGGTGTAGTCGGAGTTGTCCATAATGGTGCCAACATCCAttgag                                                                     <  2:908288/150‑5 (MQ=255)
                                                                                ggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcagca                                                                   >  1:1877817/1‑150 (MQ=255)
                                                                                 gTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcagcag                                                                  >  2:493125/1‑150 (MQ=255)
                                                                                  tGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACagcagc                                                                 >  1:4309695/1‑150 (MQ=255)
                                                                                  tGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACagcagc                                                                 <  2:2918986/150‑1 (MQ=255)
                                                                                      tttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAAc                                                             <  2:4364933/150‑1 (MQ=255)
                                                                                      tttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAAc                                                             <  2:2090417/150‑1 (MQ=255)
                                                                                       ttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAAcc                                                            >  2:653805/1‑150 (MQ=255)
                                                                                        tGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCg                                                           <  1:2160904/150‑1 (MQ=255)
                                                                                         gCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCgg                                                          >  2:3681247/1‑150 (MQ=255)
                                                                                              gTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGttt                                                     <  1:796946/150‑1 (MQ=255)
                                                                                              gTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGttt                                                     >  2:1411804/1‑150 (MQ=255)
                                                                                              gTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGttt                                                     <  1:4046706/150‑1 (MQ=255)
                                                                                               ttCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtc                                                    <  2:1003029/150‑1 (MQ=255)
                                                                                               ttCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtc                                                    <  2:696471/150‑1 (MQ=255)
                                                                                               ttCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtc                                                    <  1:3337878/150‑1 (MQ=255)
                                                                                                tCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtca                                                   <  1:1279841/150‑1 (MQ=255)
                                                                                                tCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGATTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGTTTtca                                                   <  2:2827961/150‑1 (MQ=255)
                                                                                                 cAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcat                                                  <  1:4399781/150‑1 (MQ=255)
                                                                                                  aGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                                 >  1:3653190/1‑150 (MQ=255)
                                                                                                    aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAg                                               >  2:2883774/1‑150 (MQ=255)
                                                                                                       ccACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCAc                                            >  1:1644900/1‑150 (MQ=255)
                                                                                                        cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACt                                           <  1:3249689/150‑1 (MQ=255)
                                                                                                          cGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTAc                                         >  1:1044235/1‑150 (MQ=255)
                                                                                                           gCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACa                                        <  2:2011964/150‑1 (MQ=255)
                                                                                                              aCGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAttt                                     >  2:2631467/1‑150 (MQ=255)
                                                                                                               cGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                    <  2:2403563/150‑1 (MQ=255)
                                                                                                                gggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAc                                   >  2:1835424/1‑150 (MQ=255)
                                                                                                                 ggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACt                                  <  2:3822192/150‑1 (MQ=255)
                                                                                                                  gCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTc                                 <  1:572594/150‑1 (MQ=255)
                                                                                                                  gCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTc                                 <  1:1922876/150‑1 (MQ=255)
                                                                                                                   cTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCa                                <  1:4345850/150‑1 (MQ=255)
                                                                                                                    tttttCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCAt                               <  2:1660072/150‑1 (MQ=255)
                                                                                                                      tttCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCg                             <  2:2471361/150‑1 (MQ=255)
                                                                                                                          agtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGa                         >  2:1366111/1‑150 (MQ=255)
                                                                                                                            tcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAtt                       >  1:915178/1‑150 (MQ=255)
                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                      >  2:33021/1‑150 (MQ=255)
                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                      >  2:1548000/1‑150 (MQ=255)
                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                      <  1:2797026/150‑1 (MQ=255)
                                                                                                                             cagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                      <  2:1075226/150‑1 (MQ=255)
                                                                                                                               gcgcCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTcc                    <  1:1961329/150‑1 (MQ=255)
                                                                                                                                 gccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctc                  <  1:3576650/150‑1 (MQ=255)
                                                                                                                                  ccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctct                 <  1:20984/150‑1 (MQ=255)
                                                                                                                                    gccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGt               >  2:2605529/1‑150 (MQ=255)
                                                                                                                                      cAGCGTGTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAt             <  1:1566933/150‑1 (MQ=255)
                                                                                                                                        gCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAtgt           >  1:1342902/1‑150 (MQ=255)
                                                                                                                                             tGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAcc      <  2:551859/150‑1 (MQ=255)
                                                                                                                                              gCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAccc     <  2:3043433/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAG  >  CP009273/2454148‑2454442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑