BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,471,682	A→C	18.3%	E119D (GAA→GAC)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,471,682	0	A	C	18.3%	638.6 / 165.3	283	E119D (GAA→GAC)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand): ref base A (107/124); new base C (28/24); total (135/148)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 289 total aligned reads displayed.

ATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGTTGCCAA  >  CP009273/2471533‑2471831

  cGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGt                                                                                                                                                     >  2:167116/1‑150 (MQ=255)

   ggCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTg                                                                                                                                                    >  1:1269716/1‑150 (MQ=255)

    gCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTgg                                                                                                                                                   >  1:2407447/1‑150 (MQ=255)

    gCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTgg                                                                                                                                                   <  2:2009630/150‑1 (MQ=255)

    gCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTgg                                                                                                                                                   <  1:1361532/150‑1 (MQ=255)

     ccTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTggg                                                                                                                                                  >  1:1504303/1‑150 (MQ=255)

      cTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGACTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGc                                                                                                                                                 <  2:2232841/150‑1 (MQ=255)

         ggCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGcgtc                                                                                                                                              >  2:3974606/1‑150 (MQ=255)

          gCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcgtcg                                                                                                                                             <  1:2963497/150‑1 (MQ=255)

          gCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGCGGCATCACGCTGTTTGTTGACGTGGGcgtcg                                                                                                                                             <  1:1126614/150‑1 (MQ=255)

            aCGATACTGGGAAAAATGATGGACGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTg                                                                                                                                           <  1:2253722/150‑1 (MQ=255)

               aTACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGACGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTa                                                                                                                                        >  1:2489484/1‑150 (MQ=255)

                  cTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTg                                                                                                                                     >  2:472129/1‑150 (MQ=255)

                   tGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGa                                                                                                                                    >  1:1336933/1‑150 (MQ=255)

                    gggAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGAt                                                                                                                                   >  2:3373691/1‑150 (MQ=255)

                        aaaaTGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCct                                                                                                                               <  2:1383695/150‑1 (MQ=255)

                         aaaTGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCctc                                                                                                                              >  2:272268/1‑150 (MQ=255)

                          aaTGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCctct                                                                                                                             <  1:1777172/150‑1 (MQ=255)

                           atgatgGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGAGTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTg                                                                                                                            >  2:3186291/1‑150 (MQ=255)

                            tgatgGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTgg                                                                                                                           <  1:2162182/150‑1 (MQ=255)

                                 gAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCtttt                                                                                                                      <  1:3908244/150‑1 (MQ=255)

                                        ccGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTg                                                                                                               >  2:4154407/1‑150 (MQ=255)

                                          ggggCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGcc                                                                                                             <  2:1262529/150‑1 (MQ=255)

                                          agccagacAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGcc                                                                                                             <  2:3671360/143‑1 (MQ=255)

                                            ggCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCaa                                                                                                           >  1:3350535/1‑150 (MQ=255)

                                             gCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCaaa                                                                                                          <  2:1295177/150‑1 (MQ=255)

                                               cGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCaaaaa                                                                                                        >  1:3968383/1‑150 (MQ=255)

                                               cGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCaaaaa                                                                                                        >  1:3015908/1‑150 (MQ=255)

                                                  agaaagaaTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAAc                                                                                                     >  1:2932230/1‑150 (MQ=255)

                                                    aaagaaTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCa                                                                                                   >  1:2798593/1‑150 (MQ=255)

                                                      agaaTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAAt                                                                                                 >  1:16329/1‑150 (MQ=255)

                                                       gaaTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATa                                                                                                >  1:199671/1‑150 (MQ=255)

                                                        aaTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATAc                                                                                               >  1:4085864/1‑150 (MQ=255)

                                                         aTTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATAcc                                                                                              <  1:1177051/150‑1 (MQ=255)

                                                          ttGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCt                                                                                             <  1:3435355/150‑1 (MQ=255)

                                                           tGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTc                                                                                            >  2:1731780/1‑150 (MQ=255)

                                                           tGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTc                                                                                            <  1:3498432/150‑1 (MQ=255)

                                                            ggTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGTTTTTTCAATTGCCAAAAAAACCAATACCTCa                                                                                           <  2:1049033/150‑1 (MQ=255)

                                                            ggTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCa                                                                                           <  2:3327922/150‑1 (MQ=255)

                                                             gTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTACTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCat                                                                                          >  1:638132/1‑150 (MQ=255)

                                                              tCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCatt                                                                                         <  2:4241795/150‑1 (MQ=255)

                                                              tCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCatt                                                                                         >  1:2458668/1‑150 (MQ=255)

                                                                tGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCattat                                                                                       <  1:2439153/150‑1 (MQ=255)

                                                                tGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCattat                                                                                       >  1:4009198/1‑150 (MQ=255)

                                                                  acacTTCAACGCTGCCGCTGGCTTTCAGTTGATGTGATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGATTGTTGAAGTGGGCGTCGTGCTATAGATACCTCTGGCTTTTTCAATTGCCACAAAAACCAATACCTCAttatta                                                                                     >  2:3478375/1‑150 (MQ=255)

                                                                  acacTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCAttatta                                                                                     >  2:3478344/1‑150 (MQ=255)

                                                                     cTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAg                                                                                  >  1:1356155/1‑150 (MQ=255)

                                                                      ttCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGc                                                                                 >  2:1790930/1‑150 (MQ=255)

                                                                         aaCGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTTGCCTGATTTGTGGCATCACGCAGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAATCTTg                                                                              >  1:3180371/1‑150 (MQ=255)

                                                                         aaCGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTg                                                                              <  2:2012552/150‑1 (MQ=255)

                                                                         aaCGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTAGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTg                                                                              <  2:3004462/150‑1 (MQ=255)

                                                                         aaCGCTGCCGCTGGCTTTCAGTTGATGTCAGTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTg                                                                              >  1:4407235/1‑150 (MQ=255)

                                                                                ccGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTcc                                                                       <  2:4195762/150‑1 (MQ=255)

                                                                                 cGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCg                                                                      >  2:3415792/1‑150 (MQ=255)

                                                                                 cGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCg                                                                      <  2:1556270/150‑1 (MQ=255)

                                                                                   cTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCt                                                                    <  2:2785855/150‑1 (MQ=255)

                                                                                    tGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGAGTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCATCTGGCTTTTTCAATTGCCAAAAAAACAAATACCTCATTATTAAAGCTTTCCATTACGCTa                                                                   >  2:812040/1‑150 (MQ=255)

                                                                                     ggCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTAt                                                                  <  1:3865373/150‑1 (MQ=255)

                                                                                      gCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATg                                                                 <  1:3737827/150‑1 (MQ=255)

                                                                                      gCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATg                                                                 <  2:561493/150‑1 (MQ=255)

                                                                                       cTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGt                                                                <  2:4346788/150‑1 (MQ=255)

                                                                                          tCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTAcc                                                             <  1:2520195/150‑1 (MQ=255)

                                                                                           cAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCg                                                            <  2:2696720/150‑1 (MQ=255)

                                                                                           cAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCg                                                            <  2:2903679/150‑1 (MQ=255)

                                                                                            aGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGc                                                           >  2:3030553/1‑150 (MQ=255)

                                                                                                  tGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGATTGCCATTCCGCTATGTACAGCATTGAt                                                     >  2:711879/1‑150 (MQ=255)

                                                                                                  tGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGAt                                                     >  2:3761153/1‑150 (MQ=255)

                                                                                                  tGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATAACTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGAt                                                     >  2:4441106/1‑150 (MQ=255)

                                                                                                  tGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTAGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGAt                                                     <  2:1930941/150‑1 (MQ=255)

                                                                                                    tCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATgg                                                   >  1:2629157/1‑150 (MQ=255)

                                                                                                      aTTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCa                                                 >  1:1184931/1‑150 (MQ=255)

                                                                                                        tATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGt                                               <  2:897555/150‑1 (MQ=255)

                                                                                                           ggTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCa                                            <  1:4507427/150‑1 (MQ=255)

                                                                                                           ggTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCa                                            >  2:3363950/1‑150 (MQ=255)

                                                                                                            gTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCAc                                           >  2:978145/1‑150 (MQ=255)

                                                                                                                 ggTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGt                                      >  2:1493307/1‑150 (MQ=255)

                                                                                                                  gTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTg                                     >  2:925972/1‑150 (MQ=255)

                                                                                                                     ggCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGtt                                  <  2:4504771/150‑1 (MQ=255)

                                                                                                                       ccTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTtcc                                <  1:3581982/150‑1 (MQ=255)

                                                                                                                        cTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATACCGCTATGTCCCGCATTGATGGCAGTGCACTGCGTGGTtcct                               >  2:2333045/1‑150 (MQ=255)

                                                                                                                          gATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTAGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTtcctcc                             >  1:3680756/1‑150 (MQ=255)

                                                                                                                           aTTTGTGGCATCACGCTGTTTGTTGTAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCca                            <  1:10091/150‑1 (MQ=255)

                                                                                                                              tgtgGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACAt                         >  1:827696/1‑150 (MQ=255)

                                                                                                                                tgGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATcc                       >  1:1744713/1‑150 (MQ=255)

                                                                                                                                tgGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATcc                       <  1:2102484/150‑1 (MQ=255)

                                                                                                                                tgGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATcc                       >  1:4175858/1‑150 (MQ=255)

                                                                                                                                 ggCATCACGCTGTTTGTTGAAGTGGGCGTAGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCg                      <  2:1847997/150‑1 (MQ=255)

                                                                                                                                  gCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCgg                     >  2:4039501/1‑150 (MQ=255)

                                                                                                                                    aTCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGgct                   <  1:786694/150‑1 (MQ=255)

                                                                                                                                      cACGCTGTTTGTTGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGgctgc                 >  1:2929070/1‑150 (MQ=255)

                                                                                                                                        cGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGAGTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCtt               <  2:2629157/150‑1 (MQ=255)

                                                                                                                                         gCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCttt              <  1:3200768/150‑1 (MQ=255)

                                                                                                                                         gCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCttt              <  1:947957/150‑1 (MQ=255)

                                                                                                                                           tgtttgttGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTtat            >  1:1843857/1‑150 (MQ=255)

                                                                                                                                             tttgttGACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTatat          >  1:4032301/1‑150 (MQ=255)

                                                                                                                                               tgttgAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGt        <  2:2350165/150‑1 (MQ=255)

                                                                                                                                                gttgACGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGtt       >  1:4383542/1‑150 (MQ=255)

                                                                                                                                                  tgAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGTTGc     <  1:2978862/150‑1 (MQ=255)

                                                                                                                                                  tgAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGTTGc     <  1:2760319/150‑1 (MQ=255)

                                                                                                                                                     aGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGTTGCCaa  <  2:279014/150‑1 (MQ=255)

ATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTATTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCACATCCGGCTGCTTTATATGTTGCCAA  >  CP009273/2471533‑2471831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`