Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,516,553 T→G 15.3% E264D (GAA→GAC)  xapB ← xanthosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,516,5530TG15.3% 692.0 / 127.4 279E264D (GAA→GAC) xapBxanthosine transporter
Reads supporting (aligned to +/- strand):  ref base T (121/115);  new base G (19/24);  total (140/139)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

GACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAG  >  CP009273/2516404‑2516702
                                                                                                                                                     |                                                                                                                                                     
   agcagcagcAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCt                                                                                                                                                    >  1:891456/1‑150 (MQ=255)
     cagcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGc                                                                                                                                                  >  2:2518290/1‑150 (MQ=255)
     cagcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGc                                                                                                                                                  >  1:3601944/1‑150 (MQ=255)
      agcagctAAATAAATCCGGTTGTTGACGGATCGCCATTGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGcc                                                                                                                                                 <  1:2086682/150‑1 (MQ=255)
      agcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGcc                                                                                                                                                 <  2:1401814/150‑1 (MQ=255)
         agcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATc                                                                                                                                              >  2:1597333/1‑150 (MQ=255)
          gcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCt                                                                                                                                             <  1:1777062/150‑1 (MQ=255)
           caAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCtg                                                                                                                                            <  2:1630084/150‑1 (MQ=255)
            aaaaTAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCtgt                                                                                                                                           >  1:512290/1‑150 (MQ=255)
             aaataaatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCtgtg                                                                                                                                          <  1:1127764/150‑1 (MQ=255)
                 aaatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAAc                                                                                                                                      >  1:2557278/1‑150 (MQ=255)
                   atCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTg                                                                                                                                    <  2:666671/150‑1 (MQ=255)
                      cGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACa                                                                                                                                 <  2:3193364/150‑1 (MQ=255)
                      cGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACa                                                                                                                                 >  2:1318935/1‑150 (MQ=255)
                       ggTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAg                                                                                                                                <  2:3719039/150‑1 (MQ=255)
                        gttgttGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGt                                                                                                                               >  2:2368092/1‑150 (MQ=255)
                        ggtgttgACGGATCGCCATAGGCGACGCAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAACAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGt                                                                                                                               <  2:3100457/148‑1 (MQ=255)
                         ttgttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTa                                                                                                                              <  1:2215761/150‑1 (MQ=255)
                              gACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATg                                                                                                                         >  1:2447520/1‑150 (MQ=255)
                              gACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATg                                                                                                                         >  2:2082536/1‑150 (MQ=255)
                              gACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATg                                                                                                                         <  1:996477/150‑1 (MQ=255)
                               aCGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATgg                                                                                                                        <  2:2438154/150‑1 (MQ=255)
                                cGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGa                                                                                                                       <  2:2960693/150‑1 (MQ=255)
                                 ggATCGCCATAGGCGAAGATGCCAATGCTCTGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCATATCTCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAg                                                                                                                      <  1:4402352/150‑1 (MQ=255)
                                 ggATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAg                                                                                                                      >  1:2427805/1‑150 (MQ=255)
                                   aTCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAggg                                                                                                                    >  2:2805774/1‑150 (MQ=255)
                                      gCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGata                                                                                                                 <  2:1086805/150‑1 (MQ=255)
                                                      ccAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTg                                                                                                 <  2:1184822/150‑1 (MQ=255)
                                                       cacaGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGt                                                                                                <  2:4119987/147‑1 (MQ=255)
                                                          aGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAg                                                                                             <  1:1153354/150‑1 (MQ=255)
                                                               aGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAAc                                                                                        <  2:1004375/150‑1 (MQ=255)
                                                                 cGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTc                                                                                      >  1:1919495/1‑150 (MQ=255)
                                                                 cGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTc                                                                                      <  2:3457103/150‑1 (MQ=255)
                                                                  gTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTcc                                                                                     >  2:441288/1‑150 (MQ=255)
                                                                   tCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCg                                                                                    <  1:3851132/150‑1 (MQ=255)
                                                                    ccAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCgg                                                                                   >  2:3358923/1‑150 (MQ=255)
                                                                      aGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGt                                                                                 >  2:112797/1‑150 (MQ=255)
                                                                      aGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGt                                                                                 >  1:3799003/1‑150 (MQ=255)
                                                                      aGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGt                                                                                 >  1:2922081/1‑150 (MQ=255)
                                                                      aGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGt                                                                                 >  2:3311741/1‑150 (MQ=255)
                                                                       ggCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGtt                                                                                <  1:1610972/150‑1 (MQ=255)
                                                                       ggCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGtt                                                                                <  2:4486396/150‑1 (MQ=255)
                                                                       ggCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGtt                                                                                <  2:3793780/150‑1 (MQ=255)
                                                                        gCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTa                                                                               >  1:1476305/1‑150 (MQ=255)
                                                                        gCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTa                                                                               >  2:2240528/1‑150 (MQ=255)
                                                                         ccaccaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTAc                                                                              <  1:275767/150‑1 (MQ=255)
                                                                         ccaccaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTAc                                                                              >  2:52700/1‑150 (MQ=255)
                                                                          caccaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTACTGCCATCTGTGAAACTGACAGTAAATTGGAGGGATATTTCACAACAAAACTGTCAGCAAACTCCGTGTTACg                                                                             >  2:2862520/1‑150 (MQ=255)
                                                                           accaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACgg                                                                            <  1:125825/150‑1 (MQ=255)
                                                                           accaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACgg                                                                            >  2:1519691/1‑150 (MQ=255)
                                                                                 cTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGaaa                                                                      >  2:577433/1‑150 (MQ=255)
                                                                                    atcaGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCa                                                                   >  1:3453438/1‑150 (MQ=255)
                                                                                     tcaGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCAt                                                                  >  1:1833853/1‑150 (MQ=255)
                                                                                        gCATGACGGTTTTTATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAATTGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTa                                                               <  1:3264192/150‑1 (MQ=255)
                                                                                        gCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTa                                                               <  1:4012255/150‑1 (MQ=255)
                                                                                         cATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAg                                                              <  2:3126372/150‑1 (MQ=255)
                                                                                          aTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAgg                                                             <  1:2146200/150‑1 (MQ=255)
                                                                                          aTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACCAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAgg                                                             <  2:3336509/150‑1 (MQ=255)
                                                                                          aTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAgg                                                             <  2:2992456/150‑1 (MQ=255)
                                                                                           tGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGa                                                            >  1:70669/1‑150 (MQ=255)
                                                                                              cGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACg                                                         <  1:4238683/150‑1 (MQ=255)
                                                                                               ggTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACgg                                                        <  1:1553370/150‑1 (MQ=255)
                                                                                               ggTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACgg                                                        >  1:3738497/1‑150 (MQ=255)
                                                                                                gTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGa                                                       <  1:2404603/150‑1 (MQ=255)
                                                                                                 ttttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAt                                                      <  2:2785497/150‑1 (MQ=255)
                                                                                                 ttttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAACTGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAt                                                      <  2:361494/150‑1 (MQ=255)
                                                                                                  tttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAtt                                                     <  1:1991481/150‑1 (MQ=255)
                                                                                                  tttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAtt                                                     >  2:790221/1‑150 (MQ=255)
                                                                                                     aaTGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTAcc                                                  >  1:1038882/1‑150 (MQ=255)
                                                                                                     aaTGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTAcc                                                  <  2:2249885/150‑1 (MQ=255)
                                                                                                     aaTGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTAcc                                                  <  2:2230883/150‑1 (MQ=255)
                                                                                                      aTGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCa                                                 >  2:2597263/1‑150 (MQ=255)
                                                                                                         ccAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaaaa                                              >  1:4185548/1‑150 (MQ=255)
                                                                                                         ccAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaaaa                                              >  2:586173/1‑150 (MQ=255)
                                                                                                           aaaTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAAc                                            <  2:3078005/150‑1 (MQ=255)
                                                                                                             aTCTCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGt                                          <  2:4343192/150‑1 (MQ=255)
                                                                                                              tCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGtt                                         >  2:2763739/1‑150 (MQ=255)
                                                                                                               cGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTg                                        <  1:1907616/150‑1 (MQ=255)
                                                                                                                gCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTgg                                       >  1:4079439/1‑150 (MQ=255)
                                                                                                                     aaaaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAtt                                  >  1:4253285/1‑150 (MQ=255)
                                                                                                                     aaaaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAtt                                  <  1:2023806/150‑1 (MQ=255)
                                                                                                                     aaaaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAtt                                  >  2:1934656/1‑150 (MQ=255)
                                                                                                                       aaaGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTg                                >  2:1842968/1‑150 (MQ=255)
                                                                                                                       aaaGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTg                                >  2:1747972/1‑150 (MQ=255)
                                                                                                                       aaaGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTg                                >  2:2301838/1‑150 (MQ=255)
                                                                                                                        aaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGc                               >  2:1693838/1‑150 (MQ=255)
                                                                                                                          gAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGAAATTTGCAg                             >  1:4447885/1‑150 (MQ=255)
                                                                                                                           aaTGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGt                            >  1:3879742/1‑150 (MQ=255)
                                                                                                                             tGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTAc                          <  2:3983203/150‑1 (MQ=255)
                                                                                                                               ggATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCg                        >  2:3942324/1‑150 (MQ=255)
                                                                                                                                   aGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCAcc                    >  1:3019643/1‑150 (MQ=255)
                                                                                                                                   aGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCAcc                    <  1:166205/150‑1 (MQ=255)
                                                                                                                                         tataAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcat              >  1:2378104/1‑150 (MQ=255)
                                                                                                                                          ataAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatc             <  2:3520450/150‑1 (MQ=255)
                                                                                                                                           taAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatca            >  1:615044/1‑150 (MQ=255)
                                                                                                                                            aaaGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatcat           <  2:830186/150‑1 (MQ=255)
                                                                                                                                                 ccACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCaa      >  1:828458/1‑150 (MQ=255)
                                                                                                                                                 ccACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCaa      >  1:1024063/1‑150 (MQ=255)
                                                                                                                                                 ccACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCaa      <  2:4260757/150‑1 (MQ=255)
                                                                                                                                                     ttCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAagag  <  1:3111329/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAG  >  CP009273/2516404‑2516702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑