Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,544,917 C→A 12.0% D57Y (GAT→TAT)  yfeZ ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,544,9170CA12.0% 892.5 / 72.9 262D57Y (GAT→TAT) yfeZinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (122/108);  new base A (15/17);  total (137/125)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

TTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGAT  >  CP009273/2544768‑2545066
                                                                                                                                                     |                                                                                                                                                     
ttGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATc                                                                                                                                                       >  1:3153772/1‑150 (MQ=255)
  gCCAGAAAAGTAAAACCACCTGCGCCAACATCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAACACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAgg                                                                                                                                                     <  2:343274/150‑1 (MQ=255)
   ccAGCATTGTAAAACCACCTGCGCCAACAGCAACTTAAAATTGAGTCCACGCCGGGTGCGTGGAAAGGTCGCCCGCCGCCCTATCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCggg                                                                                                                                                    <  1:1419661/150‑1 (MQ=255)
     aGCAATGTAAAACCACCTGCGCCAACAGCAACATAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGAt                                                                                                                                                  <  2:3010350/150‑1 (MQ=255)
          ttaaaacCCACCTGCGCCAACATAAACATAAAATTGCTTCCACGCCTGGTGCGTGGAAACTTAGCCCGCCGACCGCTCAAAAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAAc                                                                                                                                             <  1:252750/147‑1 (MQ=255)
          tgaaaaaCCACCGCCCCCAACAGAAAAATAAAATAGATTACACGCCTGGTGCGAGGAAACGTCGCCCGCCGACCGATCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAAc                                                                                                                                             <  1:582718/147‑1 (MQ=255)
            tAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAg                                                                                                                                           >  1:2021956/1‑150 (MQ=255)
            tAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAg                                                                                                                                           >  1:2088622/1‑150 (MQ=255)
             aaaaCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGa                                                                                                                                          >  2:1496083/1‑150 (MQ=255)
              aaaCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGAt                                                                                                                                         >  1:1356866/1‑150 (MQ=255)
               aaCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGTAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGAtt                                                                                                                                        <  2:4497943/150‑1 (MQ=255)
                 ccaccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTa                                                                                                                                      >  1:4021006/1‑150 (MQ=255)
                 ccaccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTa                                                                                                                                      >  1:1744061/1‑150 (MQ=255)
                  ccccggcgcCAACATAAAAATAAAATAGAGACCACGCCTGGTGCGTGTAAACGTAGCCCGCCGACCGATAAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTag                                                                                                                                     <  1:611566/145‑1 (MQ=255)
                  caccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTag                                                                                                                                     >  1:3193808/1‑150 (MQ=255)
                  caccTGCGCAAACAGCAACAGAAAATAGATTACCCGCCTGGTGCGAGTAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTag                                                                                                                                     <  2:2362358/150‑1 (MQ=255)
                   accTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagc                                                                                                                                    >  2:2147549/1‑150 (MQ=255)
                    ccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagca                                                                                                                                   >  2:2580277/1‑150 (MQ=255)
                    ccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagca                                                                                                                                   >  1:271792/1‑150 (MQ=255)
                    ccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagca                                                                                                                                   >  1:1351218/1‑150 (MQ=255)
                             acagcaacagAAAATAGAGAACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAAAAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTAc                                                                                                                          <  2:693849/150‑1 (MQ=255)
                              cagcaacagAAAATAGTGTACACGCCAGGTGCGTGGAAACGTAGCCCGCCGACCGCACAACAAACACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTAcc                                                                                                                         <  2:914093/150‑1 (MQ=255)
                               agcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTAccc                                                                                                                        >  1:3240427/1‑150 (MQ=255)
                                tcaaaataaaaTATAGTACACGCCTGGGGCTAGGAAACTTAGCCCGCCGACCGCTAAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCt                                                                                                                       <  1:3615914/143‑1 (MQ=255)
                                    cagAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTc                                                                                                                   >  1:3532457/1‑150 (MQ=255)
                                       aaaaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcg                                                                                                                >  2:2286570/1‑150 (MQ=255)
                                       aaaaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcg                                                                                                                >  2:1605032/1‑150 (MQ=255)
                                       aaaaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcg                                                                                                                >  1:1560877/1‑150 (MQ=255)
                                       aaaaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcg                                                                                                                >  2:2295313/1‑150 (MQ=255)
                                        aaaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcgc                                                                                                               >  1:2325266/1‑150 (MQ=255)
                                            agagTACACGCCTGGTGCGAGGAAACGTAGCCCGCCGACCGCACAACAAACACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACg                                                                                                           <  2:3297055/150‑1 (MQ=255)
                                                tACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCAcc                                                                                                       >  1:4454992/1‑150 (MQ=255)
                                                  cacGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCgg                                                                                                     >  2:2241352/1‑150 (MQ=255)
                                                   acGCCATGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACTCCAGCACCGCCGGAATGCCAGTAATAAGCCCCAGACAGAAATTATCGTGATCTGGATAAAACAGATTTAGCAGCGCAGAACCCTGCTCGCGCGACGCACCGGc                                                                                                    >  2:1251545/1‑150 (MQ=255)
                                                       cAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATg                                                                                                <  2:3586914/150‑1 (MQ=255)
                                                          gTGCGAGTAACCGTCCCCCCCCGCCCGCACAACAAACACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACa                                                                                             <  1:2299123/150‑1 (MQ=255)
                                                            gCGAGGAAACGTAGCCCGCCGCCCGCTCAACAACAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACaaa                                                                                           <  2:3760963/150‑1 (MQ=255)
                                                              gAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACa                                                                                         >  1:3789551/1‑150 (MQ=255)
                                                               aGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAg                                                                                        <  2:1199726/150‑1 (MQ=255)
                                                                ggAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGc                                                                                       <  1:2941512/150‑1 (MQ=255)
                                                                  aaaCGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCAc                                                                                     >  1:1955770/1‑150 (MQ=255)
                                                                     cGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCa                                                                                  <  2:2025822/150‑1 (MQ=255)
                                                                     cGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCa                                                                                  >  1:3143884/1‑150 (MQ=255)
                                                                       tAGCCCGCCGACCGATAAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAgg                                                                                <  1:2566611/150‑1 (MQ=255)
                                                                           ccgccgACCGCTCAAAAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTcg                                                                            <  2:412096/150‑1 (MQ=255)
                                                                            cgccgACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACACACAGCACCCAGGTTcgc                                                                           >  2:528371/1‑150 (MQ=255)
                                                                              ccgACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCgcgc                                                                         >  2:2169821/1‑150 (MQ=255)
                                                                                taCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCt                                                                       <  2:2780506/149‑1 (MQ=255)
                                                                                gACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCt                                                                       >  1:2432229/1‑150 (MQ=255)
                                                                                 aCCGCTCAACAAACACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTg                                                                      <  2:188055/150‑1 (MQ=255)
                                                                                 aCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTg                                                                      <  2:260570/150‑1 (MQ=255)
                                                                                 aCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTg                                                                      >  2:1997673/1‑150 (MQ=255)
                                                                                   cGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGaa                                                                    >  2:832114/1‑150 (MQ=255)
                                                                                    gCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAg                                                                   <  1:211166/150‑1 (MQ=255)
                                                                                     cTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGc                                                                  <  2:2750865/150‑1 (MQ=255)
                                                                                      tCAACAAACACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCa                                                                 <  1:121018/150‑1 (MQ=255)
                                                                                      tCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCa                                                                 >  2:1515112/1‑150 (MQ=255)
                                                                                       caaaaaAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCaa                                                                <  1:1403311/150‑1 (MQ=255)
                                                                                       aaaaaaacaCGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCaa                                                                <  1:1283661/146‑1 (MQ=255)
                                                                                        aacaaAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAAt                                                               <  1:2625841/150‑1 (MQ=255)
                                                                                          caaAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAg                                                             <  2:2695751/150‑1 (MQ=255)
                                                                                              aaCGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGcaca                                                         <  2:3086726/150‑1 (MQ=255)
                                                                                                   cAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCa                                                    <  1:403797/150‑1 (MQ=255)
                                                                                                       aCCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCagaag                                                >  2:1943983/1‑150 (MQ=255)
                                                                                                       aCCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCagaag                                                >  2:4220367/1‑150 (MQ=255)
                                                                                                       aCCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCagaag                                                <  2:248330/150‑1 (MQ=255)
                                                                                                        ccgccgGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCagaaga                                               <  2:4026150/150‑1 (MQ=255)
                                                                                                         cgccgGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAagag                                              <  1:760702/150‑1 (MQ=255)
                                                                                                                 tGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTa                                      <  2:3358418/150‑1 (MQ=255)
                                                                                                                 tGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTa                                      <  2:1074471/150‑1 (MQ=255)
                                                                                                                 tGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTa                                      <  1:1069489/150‑1 (MQ=255)
                                                                                                                   ccAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTaaa                                    >  1:1871978/1‑150 (MQ=255)
                                                                                                                    cAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAAc                                   <  2:2885082/150‑1 (MQ=255)
                                                                                                                     aGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACg                                  >  2:3191117/1‑150 (MQ=255)
                                                                                                                      ggAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGc                                 >  1:3763802/1‑150 (MQ=255)
                                                                                                                          tCAGCCCCAGCCAGAAATTCTCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGc                             <  1:4205085/150‑1 (MQ=255)
                                                                                                                          tCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGc                             <  1:4205053/150‑1 (MQ=255)
                                                                                                                          tCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGc                             <  1:1927730/150‑1 (MQ=255)
                                                                                                                          tCAGCCCCAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCCGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGc                             <  2:4403629/150‑1 (MQ=255)
                                                                                                                              ccccAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGAcc                         <  1:2380065/150‑1 (MQ=255)
                                                                                                                              ccccAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGAcc                         >  1:2769790/1‑150 (MQ=255)
                                                                                                                              ccccAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGAcc                         <  1:2685604/150‑1 (MQ=255)
                                                                                                                               cccAGCCAGAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCg                        >  2:3886569/1‑150 (MQ=255)
                                                                                                                                ccagccagAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCACTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGt                       >  1:3944866/1‑150 (MQ=255)
                                                                                                                                ccagccagAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGt                       <  2:246983/150‑1 (MQ=255)
                                                                                                                                    ccagAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTgcgc                   >  1:3523134/1‑150 (MQ=255)
                                                                                                                                      agAAATTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGcat                 >  1:4528514/1‑150 (MQ=255)
                                                                                                                                        aaaTTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGcatca               <  2:2171106/150‑1 (MQ=255)
                                                                                                                                        aaaTTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGcatca               >  2:1731899/1‑150 (MQ=255)
                                                                                                                                        aaaTTATCGTGATAGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGcatca               >  1:3098493/1‑150 (MQ=255)
                                                                                                                                         aaTTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCACTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGcatcat              >  2:3953922/1‑150 (MQ=255)
                                                                                                                                         aaTTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGcatcat              >  1:4241460/1‑150 (MQ=255)
                                                                                                                                          aTTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATa             >  1:3973841/1‑150 (MQ=255)
                                                                                                                                              tCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATgg         <  2:490626/150‑1 (MQ=255)
                                                                                                                                               cGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGa        <  1:2062429/150‑1 (MQ=255)
                                                                                                                                                 tGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACa      <  2:2628345/150‑1 (MQ=255)
                                                                                                                                                  gATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAg     <  2:3842224/150‑1 (MQ=255)
                                                                                                                                                   aTCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAgg    >  2:60499/1‑150 (MQ=255)
                                                                                                                                                     cGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGAt  >  2:3984927/1‑150 (MQ=255)
                                                                                                                                                     cGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGAt  <  2:3643090/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGAT  >  CP009273/2544768‑2545066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑