Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,601,887 C→A 15.9% L14L (CTC→CTA)  hyfG → hydrogenase 4, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,601,8870CA15.9% 764.3 / 96.5 245L14L (CTC→CTA) hyfGhydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base C (99/107);  new base A (22/17);  total (121/124)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 264 total aligned reads displayed.

CGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGT  >  CP009273/2601738‑2602035
                                                                                                                                                     |                                                                                                                                                    
   gcgcTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTcgcc                                                                                                                                                   <  1:1628878/150‑1 (MQ=255)
    cgcTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTcgccg                                                                                                                                                  >  2:877781/1‑150 (MQ=255)
          cACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGa                                                                                                                                            >  1:2706850/1‑150 (MQ=255)
          cACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGAATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGTAGAAACACAGTTAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGa                                                                                                                                            <  1:801957/150‑1 (MQ=255)
              ataGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAAc                                                                                                                                        <  2:448421/150‑1 (MQ=255)
              ataGTCCGCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAAc                                                                                                                                        <  2:3382541/150‑1 (MQ=255)
               taGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACg                                                                                                                                       >  2:2818010/1‑150 (MQ=255)
                aGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGc                                                                                                                                      >  2:2414285/1‑150 (MQ=255)
                  tCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCGGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAg                                                                                                                                    >  1:1466471/1‑150 (MQ=255)
                   ccTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGt                                                                                                                                   >  1:2068982/1‑150 (MQ=255)
                     tctcAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTc                                                                                                                                 <  2:52304/150‑1 (MQ=255)
                      ctcAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTcc                                                                                                                                <  1:2643593/150‑1 (MQ=255)
                       tcAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTccc                                                                                                                               >  2:4380601/1‑150 (MQ=255)
                         aGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCg                                                                                                                             <  2:3564154/150‑1 (MQ=255)
                         aGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATAGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCg                                                                                                                             >  1:4460859/1‑150 (MQ=255)
                          gggACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCgg                                                                                                                            <  1:1045143/150‑1 (MQ=255)
                            gACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGgcg                                                                                                                          <  1:582568/150‑1 (MQ=255)
                            gACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGgcg                                                                                                                          <  1:582040/150‑1 (MQ=255)
                                cACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGt                                                                                                                      >  1:1856686/1‑150 (MQ=255)
                                 aCGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTg                                                                                                                     <  1:847252/150‑1 (MQ=255)
                                     tCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTgg                                                                                                                 <  2:566187/150‑1 (MQ=255)
                                        gcctgcACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATg                                                                                                              <  1:717582/150‑1 (MQ=255)
                                          ctgcACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGaa                                                                                                            >  1:741371/1‑150 (MQ=255)
                                          ctgcACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGaa                                                                                                            <  1:1230728/150‑1 (MQ=255)
                                           tgcACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAag                                                                                                           >  2:3906448/1‑150 (MQ=255)
                                             cacaACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAagag                                                                                                         >  2:1527180/1‑150 (MQ=255)
                                             cacaACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAagag                                                                                                         <  1:461882/150‑1 (MQ=255)
                                              acaACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGACAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGc                                                                                                        >  2:1938077/1‑150 (MQ=255)
                                                      gCACCTGGCATGATTTTTTGCCTTTAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACg                                                                                                <  1:1342400/150‑1 (MQ=255)
                                                       cACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGc                                                                                               >  2:2318083/1‑150 (MQ=255)
                                                        aCCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGcc                                                                                              <  1:1491789/150‑1 (MQ=255)
                                                        aCCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGcc                                                                                              >  1:920556/1‑150 (MQ=255)
                                                        aCCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGcc                                                                                              >  1:1830640/1‑150 (MQ=255)
                                                             gCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAAc                                                                                         <  2:4022843/150‑1 (MQ=255)
                                                              cATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACa                                                                                        <  1:1214404/150‑1 (MQ=255)
                                                               aTGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAg                                                                                       >  2:2842576/1‑150 (MQ=255)
                                                                tGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAgg                                                                                      >  1:4415976/1‑150 (MQ=255)
                                                                  aTTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTc                                                                                    <  2:1576389/150‑1 (MQ=255)
                                                                  aTTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTc                                                                                    <  2:1919750/150‑1 (MQ=255)
                                                                      tttGCCTTCAGGCACCGCATCTGTGTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCa                                                                                <  2:2674938/150‑1 (MQ=255)
                                                                       ttGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCAt                                                                               <  2:3581205/150‑1 (MQ=255)
                                                                         gCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGTAGAGCGACAAACGCCTGAACAGGTCAGCATTa                                                                             >  2:2792742/1‑150 (MQ=255)
                                                                          ccTTCAGGCACCGCATCGGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTAc                                                                            <  2:3851834/150‑1 (MQ=255)
                                                                           cTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACg                                                                           <  2:3773093/150‑1 (MQ=255)
                                                                            ttCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACgg                                                                          >  2:4282746/1‑150 (MQ=255)
                                                                              cAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTg                                                                        >  1:4288136/1‑150 (MQ=255)
                                                                               aGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGa                                                                       >  1:643730/1‑150 (MQ=255)
                                                                               aGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGa                                                                       >  2:399648/1‑150 (MQ=255)
                                                                                     cGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatca                                                                 <  1:3081248/150‑1 (MQ=255)
                                                                                     cGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatca                                                                 >  1:4106492/1‑150 (MQ=255)
                                                                                       cATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaat                                                               >  2:779557/1‑150 (MQ=255)
                                                                                        aTCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaatc                                                              <  1:2818010/150‑1 (MQ=255)
                                                                                         tcggTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATct                                                             <  2:3388863/147‑1 (MQ=255)
                                                                                         tCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATct                                                             >  1:3847216/1‑150 (MQ=255)
                                                                                          cTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctg                                                            >  2:2741180/1‑150 (MQ=255)
                                                                                           tGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgc                                                           >  1:1970517/1‑150 (MQ=255)
                                                                                           tGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgc                                                           >  1:3265457/1‑150 (MQ=255)
                                                                                                 ggAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTg                                                     <  2:4424425/150‑1 (MQ=255)
                                                                                                 ggAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTg                                                     <  1:4346450/150‑1 (MQ=255)
                                                                                                     aaaCACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGt                                                 >  1:1734090/1‑150 (MQ=255)
                                                                                                     aaaCACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGt                                                 >  2:3194524/1‑150 (MQ=255)
                                                                                                        cacaGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGt                                              <  1:2118571/150‑1 (MQ=255)
                                                                                                          caGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTAc                                            >  1:1108328/1‑150 (MQ=255)
                                                                                                            gTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAg                                          <  1:1694935/150‑1 (MQ=255)
                                                                                                             tGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGt                                         <  2:110622/150‑1 (MQ=255)
                                                                                                             tGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGt                                         >  1:2420016/1‑150 (MQ=255)
                                                                                                              gAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTa                                        >  2:4212260/1‑150 (MQ=255)
                                                                                                                 cGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCt                                     <  1:4197801/150‑1 (MQ=255)
                                                                                                                 cGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCt                                     <  1:2945052/150‑1 (MQ=255)
                                                                                                                 cGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCt                                     >  2:3304829/1‑150 (MQ=255)
                                                                                                                  gTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCtt                                    >  1:3155124/1‑150 (MQ=255)
                                                                                                                   ttAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCttt                                   <  2:517619/150‑1 (MQ=255)
                                                                                                                     aaTTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttat                                 <  2:3762623/150‑1 (MQ=255)
                                                                                                                      aTTCATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttatt                                >  1:140614/1‑150 (MQ=255)
                                                                                                                       ttCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttatta                               <  2:1055955/150‑1 (MQ=255)
                                                                                                                         cATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATc                             >  1:86528/1‑150 (MQ=255)
                                                                                                                         cATCGTCAAATCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATc                             <  1:368582/150‑1 (MQ=255)
                                                                                                                          aTCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCa                            >  2:4093048/1‑150 (MQ=255)
                                                                                                                            cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAAc                          >  2:2171258/1‑150 (MQ=255)
                                                                                                                             gTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACa                         <  1:98513/150‑1 (MQ=255)
                                                                                                                              tCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACat                        <  1:3998506/150‑1 (MQ=255)
                                                                                                                              tCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACat                        <  2:2277193/150‑1 (MQ=255)
                                                                                                                               cAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatg                       <  2:4092466/150‑1 (MQ=255)
                                                                                                                                  aTCGTGGCGAAGCGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatgatg                    <  2:4427612/150‑1 (MQ=255)
                                                                                                                                   tCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGAtgg                   <  2:1315940/150‑1 (MQ=255)
                                                                                                                                     gTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGAtggct                 <  1:1266908/150‑1 (MQ=255)
                                                                                                                                       ggCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGAtggctgg               <  1:1521894/150‑1 (MQ=255)
                                                                                                                                       ggCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGAtggctgg               >  2:70207/1‑150 (MQ=255)
                                                                                                                                         cGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATggctggct             <  1:2273975/150‑1 (MQ=255)
                                                                                                                                          gAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCtt            >  2:2468867/1‑150 (MQ=255)
                                                                                                                                          gAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCtt            >  2:3996671/1‑150 (MQ=255)
                                                                                                                                           aaGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTc           >  1:426305/1‑150 (MQ=255)
                                                                                                                                            aGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGACCAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGAATCTTTATTATCAACATGATGGCTGGCTTcc          >  1:3577194/1‑150 (MQ=255)
                                                                                                                                            aGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTcc          >  1:2598985/1‑150 (MQ=255)
                                                                                                                                              cGATTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCgg        >  2:316952/1‑150 (MQ=255)
                                                                                                                                                aTTCTCGCCGCCCTGAAAACGCAGGTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTc      <  2:2645192/150‑1 (MQ=255)
                                                                                                                                                aTTCTAGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTc      >  1:2399229/1‑150 (MQ=255)
                                                                                                                                                   ctcGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTg   >  1:2964092/1‑150 (MQ=255)
                                                                                                                                                   ctcGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTg   <  1:3525937/150‑1 (MQ=255)
                                                                                                                                                    tcGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGt  >  2:3096912/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGT  >  CP009273/2601738‑2602035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑