Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,125 G→T 23.9% G218C (GGT→TGT)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,1250GT23.9% 673.6 / 181.5 279G218C (GGT→TGT) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (96/116);  new base T (34/33);  total (130/149)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

GTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCG  >  CP009273/2618976‑2619273
                                                                                                                                                     |                                                                                                                                                    
   gtgtCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTa                                                                                                                                                   >  2:2494441/1‑150 (MQ=255)
    tgtCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTAc                                                                                                                                                  >  1:2565881/1‑150 (MQ=255)
     gtCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTAcc                                                                                                                                                 >  1:1081726/1‑150 (MQ=255)
      tCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCa                                                                                                                                                >  1:3406181/1‑150 (MQ=255)
       cAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCAt                                                                                                                                               <  2:3889049/150‑1 (MQ=255)
        aGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATa                                                                                                                                              >  2:4093880/1‑150 (MQ=255)
          cTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaa                                                                                                                                            >  1:3640089/1‑150 (MQ=255)
           tttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaaa                                                                                                                                           <  2:4520854/150‑1 (MQ=255)
            ttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAg                                                                                                                                          <  1:2766428/150‑1 (MQ=255)
             tGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTCCCATAAAAgc                                                                                                                                         >  2:4416326/1‑150 (MQ=255)
               cccAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccg                                                                                                                                       <  1:1658836/150‑1 (MQ=255)
               cccAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccg                                                                                                                                       <  1:3851288/150‑1 (MQ=255)
                 cAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAgccgcc                                                                                                                                     <  1:2459150/150‑1 (MQ=255)
                   gCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCa                                                                                                                                   >  2:629575/1‑150 (MQ=255)
                    cTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCAt                                                                                                                                  >  1:51281/1‑150 (MQ=255)
                     tttATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATg                                                                                                                                 <  2:4405517/150‑1 (MQ=255)
                        aTTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAg                                                                                                                              >  1:471449/1‑150 (MQ=255)
                          tttCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAgtg                                                                                                                            <  1:1336949/150‑1 (MQ=255)
                           ttCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAgtgt                                                                                                                           <  1:2106070/150‑1 (MQ=255)
                              cTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTaa                                                                                                                        >  2:1214311/1‑150 (MQ=255)
                              cTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTaa                                                                                                                        >  2:3658734/1‑150 (MQ=255)
                              cTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTaa                                                                                                                        >  2:205247/1‑150 (MQ=255)
                               tGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCAGGAAGTGTTAAt                                                                                                                       >  2:1728082/1‑150 (MQ=255)
                               tGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCATATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAAt                                                                                                                       >  2:2182104/1‑150 (MQ=255)
                                ggCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATg                                                                                                                      <  2:1909007/150‑1 (MQ=255)
                                   ggggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGaa                                                                                                                   <  2:1392856/150‑1 (MQ=255)
                                     ggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAAt                                                                                                                 >  1:995702/1‑150 (MQ=255)
                                     ggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAAt                                                                                                                 >  1:2162460/1‑150 (MQ=255)
                                          tcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCg                                                                                                            >  1:1677904/1‑150 (MQ=255)
                                           caATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCga                                                                                                           <  2:3992977/150‑1 (MQ=255)
                                            aaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgag                                                                                                          >  2:1397803/1‑150 (MQ=255)
                                            aaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgag                                                                                                          >  2:3680673/1‑150 (MQ=255)
                                             aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                         >  1:1197325/1‑150 (MQ=255)
                                             aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                         <  1:1477509/150‑1 (MQ=255)
                                              tAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaa                                                                                                        <  2:2151172/150‑1 (MQ=255)
                                              tAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaa                                                                                                        <  2:3442960/150‑1 (MQ=255)
                                              tAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAAGTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaa                                                                                                        <  2:2151036/150‑1 (MQ=255)
                                                    gaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACgg                                                                                                  <  1:2351938/150‑1 (MQ=255)
                                                    gaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACgg                                                                                                  <  1:3254176/150‑1 (MQ=255)
                                                    gaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACgg                                                                                                  >  2:604473/1‑150 (MQ=255)
                                                      aTTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCAGGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGa                                                                                                >  2:2927555/1‑150 (MQ=255)
                                                        tttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATa                                                                                              <  2:3592313/150‑1 (MQ=255)
                                                        tttCAGCGCGCGCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATa                                                                                              <  2:827776/150‑1 (MQ=255)
                                                        tttCAGCGCGCGCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATa                                                                                              <  2:1774578/150‑1 (MQ=255)
                                                            aGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTa                                                                                          <  2:2722133/150‑1 (MQ=255)
                                                            aGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTa                                                                                          >  1:1411988/1‑150 (MQ=255)
                                                              cgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAcc                                                                                        >  2:1101921/1‑150 (MQ=255)
                                                                cgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAcccc                                                                                      <  1:4400377/150‑1 (MQ=255)
                                                                 gcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                                     >  1:4375416/1‑150 (MQ=255)
                                                                     gCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAAc                                                                                 <  1:817786/150‑1 (MQ=255)
                                                                     gCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAAc                                                                                 <  1:3552116/150‑1 (MQ=255)
                                                                      cATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                                >  2:2417003/1‑150 (MQ=255)
                                                                       aTGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGt                                                                               >  1:1717533/1‑150 (MQ=255)
                                                                         ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                             >  2:4167690/1‑150 (MQ=255)
                                                                         ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                             <  1:2073917/150‑1 (MQ=255)
                                                                          gcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTg                                                                            <  2:4053100/150‑1 (MQ=255)
                                                                            ggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAAGGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGa                                                                          <  1:409330/150‑1 (MQ=255)
                                                                              cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaa                                                                        <  1:3757459/150‑1 (MQ=255)
                                                                              cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaa                                                                        <  1:2435250/150‑1 (MQ=255)
                                                                                    aaaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTgg                                                                  <  2:2702612/150‑1 (MQ=255)
                                                                                        aCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaaa                                                              <  2:669611/150‑1 (MQ=255)
                                                                                          tGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaag                                                            <  2:3518741/150‑1 (MQ=255)
                                                                                          tGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaag                                                            <  2:2822017/150‑1 (MQ=255)
                                                                                                cTTTAACCTGGCAATTCCGTAGTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGttt                                                      <  2:1470528/150‑1 (MQ=255)
                                                                                                   tAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAc                                                   >  1:3093961/1‑150 (MQ=255)
                                                                                                    aaCCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACg                                                  >  1:4318334/1‑150 (MQ=255)
                                                                                                      ccTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGACATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTc                                                >  1:3330738/1‑150 (MQ=255)
                                                                                                      ccTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTc                                                >  1:2017308/1‑150 (MQ=255)
                                                                                                       cTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCa                                               <  1:4244647/150‑1 (MQ=255)
                                                                                                          gCAAGTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCg                                            <  2:2402044/150‑1 (MQ=255)
                                                                                                           cAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGt                                           >  2:4364052/1‑150 (MQ=255)
                                                                                                              ttCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAt                                        >  2:305232/1‑150 (MQ=255)
                                                                                                              ttCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAt                                        >  2:3275213/1‑150 (MQ=255)
                                                                                                               tCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAtt                                       <  1:2600386/150‑1 (MQ=255)
                                                                                                                  gTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCg                                    >  1:2267242/1‑150 (MQ=255)
                                                                                                                    aTTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCa                                  >  2:2325803/1‑150 (MQ=255)
                                                                                                                       cAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCg                               <  1:411090/150‑1 (MQ=255)
                                                                                                                         gggCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCt                             >  1:1297097/1‑150 (MQ=255)
                                                                                                                         gggCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCt                             >  1:2135087/1‑150 (MQ=255)
                                                                                                                         gggCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCt                             <  2:2758675/150‑1 (MQ=255)
                                                                                                                            cTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAg                          <  1:3807475/150‑1 (MQ=255)
                                                                                                                             tGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGt                         <  2:4194888/150‑1 (MQ=255)
                                                                                                                              ggAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt                        <  2:84523/150‑1 (MQ=255)
                                                                                                                              ggAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt                        >  2:3104497/1‑150 (MQ=255)
                                                                                                                              ggAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt                        <  1:2778101/150‑1 (MQ=255)
                                                                                                                               gAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGttt                       <  2:1527633/150‑1 (MQ=255)
                                                                                                                                  cGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTAcc                    <  2:1305232/150‑1 (MQ=255)
                                                                                                                                  cGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTAcc                    <  2:308457/150‑1 (MQ=255)
                                                                                                                                  cGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTAcc                    <  1:3935832/150‑1 (MQ=255)
                                                                                                                                    ttGCAATGGGCGCTTCCGGTACCATAACAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATGACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCgg                  <  1:895167/150‑1 (MQ=255)
                                                                                                                                    ttGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCgg                  <  1:196592/150‑1 (MQ=255)
                                                                                                                                      gCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGt                <  2:2912865/150‑1 (MQ=255)
                                                                                                                                        aaTGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCt              >  2:422902/1‑150 (MQ=255)
                                                                                                                                         aTGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCtt             <  2:2455982/150‑1 (MQ=255)
                                                                                                                                                cTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAag      <  2:4459844/150‑1 (MQ=255)
                                                                                                                                                  tCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAagag    >  1:2280818/1‑150 (MQ=255)
                                                                                                                                                   ccGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGc   <  2:1679897/150‑1 (MQ=255)
                                                                                                                                                    cGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCg  <  1:2947821/150‑1 (MQ=255)
                                                                                                                                                    cGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCg  <  1:2947390/150‑1 (MQ=255)
                                                                                                                                                    cGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCg  <  1:1099953/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCG  >  CP009273/2618976‑2619273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑