Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,687,651 C→A 6.4% G418C (GGC→TGC)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,687,6510CA 6.4% 828.1 / 23.4 218G418C (GGC→TGC) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (112/91);  new base A (6/9);  total (118/100)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

AAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCTT  >  CP009273/2687502‑2687800
                                                                                                                                                     |                                                                                                                                                     
aaaGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGcc                                                                                                                                                       >  2:2991303/1‑150 (MQ=255)
  aGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGa                                                                                                                                                     >  1:3051953/1‑150 (MQ=255)
    tCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATc                                                                                                                                                   <  1:2503671/150‑1 (MQ=255)
      gAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATccc                                                                                                                                                 >  1:3153250/1‑150 (MQ=255)
       aGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATcccg                                                                                                                                                <  1:3816927/150‑1 (MQ=255)
       aGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATcccg                                                                                                                                                <  1:541864/150‑1 (MQ=255)
       aGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATcccg                                                                                                                                                >  1:4346166/1‑150 (MQ=255)
              cATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCa                                                                                                                                         <  2:2356984/150‑1 (MQ=255)
                 cAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGACTGTTGCCGATCCCGCCCGCCCGCa                                                                                                                                      >  1:4129224/1‑150 (MQ=255)
                 cAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGACTGTTGCCGATCCCGCCCGCCAGCa                                                                                                                                      >  1:4129608/1‑150 (MQ=255)
                  aGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTACCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCAt                                                                                                                                     <  2:4490669/150‑1 (MQ=255)
                     cTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGAt                                                                                                                                  >  1:749542/1‑150 (MQ=255)
                      tGACCAGACGCCATAGAAGACGCTGCACCACCGCCACGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATc                                                                                                                                 <  2:680031/150‑1 (MQ=255)
                      tGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATc                                                                                                                                 >  2:2309699/1‑150 (MQ=255)
                         ccAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGACTGTTGCCGATCCCGCCCGCCAGCATGATCGGt                                                                                                                              >  2:2380839/1‑150 (MQ=255)
                         ccAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGt                                                                                                                              >  2:305894/1‑150 (MQ=255)
                            gACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGTCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTtg                                                                                                                           >  2:220762/1‑150 (MQ=255)
                            gACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTtg                                                                                                                           >  1:325528/1‑150 (MQ=255)
                               gCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGa                                                                                                                        >  1:3372457/1‑150 (MQ=255)
                                 cATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATa                                                                                                                      >  1:453095/1‑150 (MQ=255)
                                 cATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCAGTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATa                                                                                                                      <  2:2133126/150‑1 (MQ=255)
                                   tAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGACTGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAAc                                                                                                                    >  2:2602766/1‑150 (MQ=255)
                                    agaagaCGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAAcc                                                                                                                   >  2:4127691/1‑150 (MQ=255)
                                    agaagaCGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAAcc                                                                                                                   >  1:1663173/1‑150 (MQ=255)
                                    agaagaCGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAAcc                                                                                                                   >  1:2316667/1‑150 (MQ=255)
                                       agaCGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACcgcg                                                                                                                >  1:3296020/1‑150 (MQ=255)
                                          cGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAg                                                                                                             <  1:2990789/150‑1 (MQ=255)
                                          cGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAg                                                                                                             <  1:344474/150‑1 (MQ=255)
                                            cTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGct                                                                                                           >  1:3342967/1‑150 (MQ=255)
                                             tGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctc                                                                                                          <  2:3517353/150‑1 (MQ=255)
                                             tGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctc                                                                                                          <  2:2163669/150‑1 (MQ=255)
                                              gCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctct                                                                                                         <  2:2227068/150‑1 (MQ=255)
                                                  caccGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGc                                                                                                     >  2:1617481/1‑150 (MQ=255)
                                                      gCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGtt                                                                                                 >  2:1462735/1‑150 (MQ=255)
                                                       ccAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTtg                                                                                                <  2:2470560/150‑1 (MQ=255)
                                                         aaGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCGTCGCACCGACGTTGATCTCGCCTTTTTGGACGTGATCGGCGCGAATGTTGCAGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTtgtg                                                                                              <  2:4451875/150‑1 (MQ=255)
                                                            aCCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCt                                                                                           <  1:433575/150‑1 (MQ=255)
                                                             ccGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTg                                                                                          <  2:1546485/150‑1 (MQ=255)
                                                              cGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt                                                                                         <  2:235946/150‑1 (MQ=255)
                                                              cGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCAGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt                                                                                         >  1:4081858/1‑150 (MQ=255)
                                                              cGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCAGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt                                                                                         >  1:1805896/1‑150 (MQ=255)
                                                                aTGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTc                                                                                       >  2:1561222/1‑150 (MQ=255)
                                                                  gTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCAc                                                                                     <  2:936268/150‑1 (MQ=255)
                                                                   ttCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACt                                                                                    <  2:557152/150‑1 (MQ=255)
                                                                       ttGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACttttt                                                                                <  1:351839/150‑1 (MQ=255)
                                                                       ttGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACttttt                                                                                <  1:2417570/150‑1 (MQ=255)
                                                                       ttGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACttttt                                                                                <  1:449579/150‑1 (MQ=255)
                                                                        tGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttc                                                                               >  2:883562/1‑150 (MQ=255)
                                                                        tGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCAGGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttc                                                                               >  2:1313/1‑150 (MQ=255)
                                                                         gCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttct                                                                              >  1:1516802/1‑150 (MQ=255)
                                                                         gCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttct                                                                              >  1:118155/1‑150 (MQ=255)
                                                                         gCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttct                                                                              <  2:184841/150‑1 (MQ=255)
                                                                          ccGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttctt                                                                             <  2:611484/150‑1 (MQ=255)
                                                                                   cgAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGAGCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATCAGTAc                                                                    >  2:1646364/1‑150 (MQ=255)
                                                                                    gagaACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACg                                                                   >  2:3805985/1‑150 (MQ=255)
                                                                                    gagaACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACg                                                                   >  2:3317441/1‑150 (MQ=255)
                                                                                    gagaACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACg                                                                   >  2:1528290/1‑150 (MQ=255)
                                                                                    gagaACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACg                                                                   >  1:80426/1‑150 (MQ=255)
                                                                                     agaACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACgg                                                                  <  2:2914189/150‑1 (MQ=255)
                                                                                            ccAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGc                                                           >  2:1697802/1‑150 (MQ=255)
                                                                                            ccAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGc                                                           >  2:3156522/1‑150 (MQ=255)
                                                                                            ccAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGc                                                           >  2:1381008/1‑150 (MQ=255)
                                                                                               gCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGt                                                        >  1:1434254/1‑150 (MQ=255)
                                                                                               gCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGt                                                        >  2:4288867/1‑150 (MQ=255)
                                                                                                cTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGtt                                                       <  2:4068502/150‑1 (MQ=255)
                                                                                                cTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGtt                                                       <  1:1838976/150‑1 (MQ=255)
                                                                                                 ttCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTc                                                      <  1:3914217/150‑1 (MQ=255)
                                                                                                  tCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCa                                                     >  1:1636623/1‑150 (MQ=255)
                                                                                                    gCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGACTGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGt                                                   <  1:3916417/150‑1 (MQ=255)
                                                                                                     cACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCAGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTg                                                  <  2:750034/150‑1 (MQ=255)
                                                                                                      aCCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGCATGTTGCCGATCCCGCCCGCCAGCATGATCGGGTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGGTCAGTGc                                                 >  1:1971581/1‑150 (MQ=255)
                                                                                                          aCGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGga                                             >  2:1933456/1‑150 (MQ=255)
                                                                                                          aCGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGGTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGga                                             <  1:2120855/150‑1 (MQ=255)
                                                                                                            gTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGgacg                                           <  2:2832935/150‑1 (MQ=255)
                                                                                                             ttGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGgacga                                          >  1:2071833/1‑150 (MQ=255)
                                                                                                              tGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGgacgac                                         <  2:817940/150‑1 (MQ=255)
                                                                                                              tGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGgacgac                                         >  1:1126512/1‑150 (MQ=255)
                                                                                                              tGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGATGTGGCTGTTCACTTTTTCTTCATAAGTAAGGACGAATCCGATCAGTGCCGgacgac                                         >  1:3412629/1‑150 (MQ=255)
                                                                                                               gATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGAcc                                        >  2:3972195/1‑150 (MQ=255)
                                                                                                                aTCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGCTCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCa                                       <  2:3684487/150‑1 (MQ=255)
                                                                                                                aTCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCa                                       >  2:2817625/1‑150 (MQ=255)
                                                                                                                  ctcGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCaaa                                     <  1:2588794/150‑1 (MQ=255)
                                                                                                                    cGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAAtt                                   >  1:543914/1‑150 (MQ=255)
                                                                                                                       cTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCgt                                >  1:1435101/1‑150 (MQ=255)
                                                                                                                         ttttGTACGTGATCGGCGCGAATGTTGCAGATCCCGCCCGCCAGCATGACCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACCTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCgttg                              <  1:1005600/150‑1 (MQ=255)
                                                                                                                             gTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTaa                          >  1:1016667/1‑150 (MQ=255)
                                                                                                                              tACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTaaa                         >  2:3849626/1‑150 (MQ=255)
                                                                                                                                cGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAAcg                       >  2:354184/1‑150 (MQ=255)
                                                                                                                                  tGATCGGCGCGAATGTTGCAGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAAcgcc                     >  2:1193692/1‑150 (MQ=255)
                                                                                                                                   gATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAAcgccg                    >  1:2773067/1‑150 (MQ=255)
                                                                                                                                       ggCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAAcgccgcgcc                >  1:844364/1‑150 (MQ=255)
                                                                                                                                        gcgcGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAAcgccgcgccg               <  1:89740/150‑1 (MQ=255)
                                                                                                                                           cgAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGccc            >  2:3874820/1‑150 (MQ=255)
                                                                                                                                           cgAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGccc            >  2:1593137/1‑150 (MQ=255)
                                                                                                                                            gAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCa           >  1:1085476/1‑150 (MQ=255)
                                                                                                                                             aaTGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAg          <  1:3730091/150‑1 (MQ=255)
                                                                                                                                              aTGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGc         >  2:1994091/1‑150 (MQ=255)
                                                                                                                                                     cGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCtt  <  1:4357481/150‑1 (MQ=255)
                                                                                                                                                     cGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCtt  >  2:465789/1‑150 (MQ=255)
                                                                                                                                                     cGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCtg  >  1:4024198/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTTCAGTGCCGGACGACCAAATTCGTTGTTAAACGCCGCGCCGCCCAGCGGGCCTT  >  CP009273/2687502‑2687800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑