Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,733,7530CT81.2% 569.2 / 123.8 234E253K (GAG→AAG) aroF3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base C (28/16);  new base T (106/84);  total (134/100)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 254 total aligned reads displayed.

TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCG  >  CP009273/2733606‑2733901
                                                                                                                                                    |                                                                                                                                                    
tcatcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑tttt                                                                                                                                                    >  1:2662507/1‑150 (MQ=255)
 catcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑Ttttt                                                                                                                                                   >  2:858784/1‑150 (MQ=255)
   tcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑Ttttttt                                                                                                                                                 <  1:2530839/150‑1 (MQ=255)
   tcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑Ttttttt                                                                                                                                                 <  1:2062577/150‑1 (MQ=255)
   tcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACAGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑Ttttttt                                                                                                                                                 >  2:1574597/1‑150 (MQ=255)
    caGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTc                                                                                                                                                <  2:2580265/150‑1 (MQ=255)
      gACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTcac                                                                                                                                              <  1:954038/150‑1 (MQ=255)
         cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACAtt                                                                                                                                           >  1:1170118/1‑150 (MQ=255)
         cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACAtt                                                                                                                                           >  2:2233080/1‑150 (MQ=255)
          aataatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTg                                                                                                                                          <  1:3805869/150‑1 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTgcg                                                                                                                                        >  2:1940417/1‑150 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTgcg                                                                                                                                        >  1:4375055/1‑150 (MQ=255)
             aatTGAGCGATTGCCATCTTTGATTTGAGTAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTgcgc                                                                                                                                       >  1:4317922/1‑150 (MQ=255)
                tGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAAc                                                                                                                                    >  1:342235/1‑150 (MQ=255)
                      aTTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGc                                                                                                                              >  1:972646/1‑150 (MQ=255)
                        tGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTTCACATTGCGCAACATCCGCa                                                                                                                             >  1:2592827/1‑150 (MQ=255)
                            aTCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCt                                                                                                                        >  1:2234718/1‑150 (MQ=255)
                             tCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                       <  1:3326748/150‑1 (MQ=255)
                             tCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                       <  1:1727433/150‑1 (MQ=255)
                              cTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCtat                                                                                                                      <  1:863302/150‑1 (MQ=255)
                                ttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAg                                                                                                                    <  1:3024976/150‑1 (MQ=255)
                                    tttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCg                                                                                                                <  2:2340254/150‑1 (MQ=255)
                                     ttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCgg                                                                                                               <  2:2295037/150‑1 (MQ=255)
                                        aGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgcgc                                                                                                            >  2:293411/1‑150 (MQ=255)
                                        aGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGGTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgcgc                                                                                                            >  2:294276/1‑150 (MQ=255)
                                           aaCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                         <  1:3105406/150‑1 (MQ=255)
                                           aaCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                         <  1:322630/150‑1 (MQ=255)
                                             ccACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTac                                                                                                       <  2:821600/150‑1 (MQ=255)
                                                cGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccac                                                                                                    >  1:1800007/1‑150 (MQ=255)
                                                  gATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcg                                                                                                  <  2:168415/150‑1 (MQ=255)
                                                       tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCCGGGCTATAGTTCGGCGCTTTACCACCGCGCAg                                                                                             >  1:3151417/1‑150 (MQ=255)
                                                        gCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAgg                                                                                            >  1:65827/1‑150 (MQ=255)
                                                         ccACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGa                                                                                           <  1:1897037/150‑1 (MQ=255)
                                                          cACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGAt                                                                                          >  1:4124939/1‑150 (MQ=255)
                                                           aCCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATc                                                                                         <  1:3466705/150‑1 (MQ=255)
                                                           aCCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATc                                                                                         >  1:4446442/1‑150 (MQ=255)
                                                            ccGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATca                                                                                        <  2:3410844/150‑1 (MQ=255)
                                                            ccGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATca                                                                                        <  2:610107/150‑1 (MQ=255)
                                                              gCAGGCTGACGGCGATACTCTTTATTGGATTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTGTTTTCACATTCCGCAACCTCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcaca                                                                                      <  2:1334581/150‑1 (MQ=255)
                                                               cAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACAt                                                                                     <  2:3611235/150‑1 (MQ=255)
                                                               cAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACAt                                                                                     <  1:565044/150‑1 (MQ=255)
                                                               cAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACAt                                                                                     >  2:350498/1‑150 (MQ=255)
                                                                aGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                    >  1:3406988/1‑150 (MQ=255)
                                                                aGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                    >  2:698357/1‑150 (MQ=255)
                                                                aGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                    >  1:3767144/1‑150 (MQ=255)
                                                                aGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                    <  1:4015117/150‑1 (MQ=255)
                                                                  gCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGc                                                                                  >  1:195245/1‑150 (MQ=255)
                                                                    tGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGTCGCTGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGTCGCTTTACCAACGCGCAGGATCACATGGCCg                                                                                <  2:3198354/150‑1 (MQ=255)
                                                                           gATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCAAATGGCCGTCCGGAt                                                                         >  2:560182/1‑150 (MQ=255)
                                                                            aTAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGCCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGACGTCCGGAtt                                                                        >  2:255608/1‑150 (MQ=255)
                                                                             tAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTc                                                                       >  2:2910243/1‑150 (MQ=255)
                                                                             tAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTc                                                                       <  2:1977641/150‑1 (MQ=255)
                                                                             tAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTATCACATTGCGCAACCTCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAATc                                                                       >  2:3790555/1‑150 (MQ=255)
                                                                             tAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATAAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTc                                                                       <  2:2177836/150‑1 (MQ=255)
                                                                              aaTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTcc                                                                      >  1:2895673/1‑150 (MQ=255)
                                                                              aaTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTcc                                                                      >  2:221411/1‑150 (MQ=255)
                                                                               aTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTccc                                                                     >  1:4131162/1‑150 (MQ=255)
                                                                                 cTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCt                                                                   >  2:2220045/1‑150 (MQ=255)
                                                                                  tttATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTg                                                                  >  1:544313/1‑150 (MQ=255)
                                                                                  tttATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTg                                                                  <  1:3807100/150‑1 (MQ=255)
                                                                                     aTTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGt                                                               <  1:4058927/150‑1 (MQ=255)
                                                                                      ttGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGtt                                                              <  1:2269271/150‑1 (MQ=255)
                                                                                          aaTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa                                                          >  1:4490808/1‑150 (MQ=255)
                                                                                           aTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAg                                                         >  1:2321270/1‑150 (MQ=255)
                                                                                                 gTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgc                                                   <  2:2064599/150‑1 (MQ=255)
                                                                                                     cTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcc                                               <  2:1283739/150‑1 (MQ=255)
                                                                                                     cTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcc                                               >  1:3545720/1‑150 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                              >  2:1501651/1‑150 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                              <  2:806293/150‑1 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                              >  2:4308991/1‑150 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                              <  2:4229369/150‑1 (MQ=255)
                                                                                                         aaTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc                                           >  1:2203419/1‑150 (MQ=255)
                                                                                                         aaTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc                                           >  2:2748663/1‑150 (MQ=255)
                                                                                                            cTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctg                                        >  1:3618158/1‑150 (MQ=255)
                                                                                                             tACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc                                       >  2:2284553/1‑150 (MQ=255)
                                                                                                              aCCCTCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACctgccctgcc                                      <  1:2976567/150‑1 (MQ=255)
                                                                                                                  tCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGt                                  >  1:1413308/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                                >  2:3230850/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                                >  2:4458876/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                                >  1:145353/1‑150 (MQ=255)
                                                                                                                     gagaCGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTaa                               >  2:394092/1‑150 (MQ=255)
                                                                                                                     gagaCGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTaa                               >  2:2366471/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                             >  1:1083472/1‑150 (MQ=255)
                                                                                                                          gggCGCAGTCCCGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCca                          >  2:143353/1‑150 (MQ=255)
                                                                                                                                aGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                    >  2:2646244/1‑150 (MQ=255)
                                                                                                                                   cccGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg                 >  2:3294951/1‑150 (MQ=255)
                                                                                                                                   cccGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg                 >  1:363581/1‑150 (MQ=255)
                                                                                                                                     cGCCTGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTg               <  2:145353/150‑1 (MQ=255)
                                                                                                                                     cGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTg               <  1:1537629/150‑1 (MQ=255)
                                                                                                                                     cGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTg               <  1:2832339/150‑1 (MQ=255)
                                                                                                                                         tGTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGc           >  2:4026275/1‑150 (MQ=255)
                                                                                                                                          gTTCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCt          <  1:2448042/150‑1 (MQ=255)
                                                                                                                                           ttCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTg         <  2:2662507/150‑1 (MQ=255)
                                                                                                                                           ttCCATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTg         <  1:80279/150‑1 (MQ=255)
                                                                                                                                              cATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc      >  2:1374330/1‑150 (MQ=255)
                                                                                                                                              cATC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc      >  2:3524910/1‑150 (MQ=255)
                                                                                                                                              cATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc      >  1:2113407/1‑150 (MQ=255)
                                                                                                                                               aTC‑TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcg     <  1:2807032/150‑1 (MQ=255)
                                                                                                                                                tc‑ttTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcgg    >  1:1321197/1‑150 (MQ=255)
                                                                                                                                                   tctTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcggcg  <  1:2277762/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATC‑TCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCG  >  CP009273/2733606‑2733901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑