Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,779,067 C→A 86.1% intergenic (‑697/+54) ypjC ← / ← ileY pseudogene/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,779,0670CA86.1% 557.1 / 86.5 214intergenic (‑697/+54)ypjC/ileYpseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base C (13/17);  new base A (91/93);  total (104/110)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

CATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA  >  CP009273/2778918‑2779213
                                                                                                                                                     |                                                                                                                                                  
cATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGAcc                                                                                                                                                    <  1:3578750/150‑1 (MQ=255)
   aGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGcc                                                                                                                                                 >  1:2303921/1‑150 (MQ=255)
     cAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAt                                                                                                                                               <  2:1091688/150‑1 (MQ=255)
     cAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAt                                                                                                                                               >  2:1133869/1‑150 (MQ=255)
      aGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATc                                                                                                                                              >  1:4177617/1‑150 (MQ=255)
           ttttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTa                                                                                                                                         <  1:2745225/150‑1 (MQ=255)
           ttttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTa                                                                                                                                         >  2:812691/1‑150 (MQ=255)
           ttttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTa                                                                                                                                         <  2:4248454/150‑1 (MQ=255)
               ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcccc                                                                                                                                     >  2:2495823/1‑150 (MQ=255)
                 ccAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTCCCCCtt                                                                                                                                   >  2:2799130/1‑150 (MQ=255)
                 ccAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCtt                                                                                                                                   >  2:3699898/1‑150 (MQ=255)
                 ccAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCtt                                                                                                                                   >  2:1186187/1‑150 (MQ=255)
                  cAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTg                                                                                                                                  <  2:1107627/150‑1 (MQ=255)
                  cAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTg                                                                                                                                  >  1:4470536/1‑150 (MQ=255)
                    ggTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTa                                                                                                                                >  2:814846/1‑150 (MQ=255)
                    ggTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTa                                                                                                                                >  1:3065121/1‑150 (MQ=255)
                       aCTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTAccc                                                                                                                             <  1:1382004/150‑1 (MQ=255)
                          tttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTa                                                                                                                          <  1:34505/150‑1 (MQ=255)
                           ttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTaa                                                                                                                         >  2:2274511/1‑150 (MQ=255)
                             gAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                       <  2:2497118/150‑1 (MQ=255)
                             gAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                       >  2:2069757/1‑150 (MQ=255)
                             gAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                       <  1:2535328/150‑1 (MQ=255)
                               aTGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAActct                                                                                                                     >  1:3954660/1‑150 (MQ=255)
                                tGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCtt                                                                                                                    <  2:3545077/150‑1 (MQ=255)
                                   gTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCt                                                                                                                 <  1:3004733/150‑1 (MQ=255)
                                   gTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCt                                                                                                                 <  1:461860/150‑1 (MQ=255)
                                    tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                <  1:2490083/150‑1 (MQ=255)
                                    tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                <  2:3218155/150‑1 (MQ=255)
                                      cTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGAt                                                                                                              <  2:877566/150‑1 (MQ=255)
                                          tGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGt                                                                                                          <  2:1940773/150‑1 (MQ=255)
                                           ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTa                                                                                                         <  1:818941/150‑1 (MQ=255)
                                           ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTa                                                                                                         >  2:3611500/1‑150 (MQ=255)
                                           ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTa                                                                                                         >  2:1324483/1‑150 (MQ=255)
                                                aaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataa                                                                                                    >  1:3996747/1‑150 (MQ=255)
                                                     aTTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAtt                                                                                               >  1:4418102/1‑150 (MQ=255)
                                                      ttGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAttt                                                                                              <  2:2927129/150‑1 (MQ=255)
                                                         cAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTCAACTCTTCCTGATACGTAAATAATGATTtggt                                                                                           <  1:1593067/150‑1 (MQ=255)
                                                           gTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggtgg                                                                                         <  2:932709/150‑1 (MQ=255)
                                                            tGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGc                                                                                        >  2:1642901/1‑150 (MQ=255)
                                                             ggCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCTCCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGcc                                                                                       <  2:2524715/150‑1 (MQ=255)
                                                                gTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCtt                                                                                    >  1:3097483/1‑150 (MQ=255)
                                                                gTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCtt                                                                                    >  2:258014/1‑150 (MQ=255)
                                                                gTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCtt                                                                                    >  2:3666154/1‑150 (MQ=255)
                                                                gTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCtt                                                                                    <  1:1993559/150‑1 (MQ=255)
                                                                 tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                   <  2:20025/150‑1 (MQ=255)
                                                                 tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                   <  1:2633159/150‑1 (MQ=255)
                                                                   ccACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCt                                                                                 >  2:3023104/1‑150 (MQ=255)
                                                                    cACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTg                                                                                >  1:36341/1‑150 (MQ=255)
                                                                     aCGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTgg                                                                               <  2:4092830/150‑1 (MQ=255)
                                                                     aCGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTgg                                                                               <  2:1814798/150‑1 (MQ=255)
                                                                     aCGGACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTgg                                                                               <  2:578558/150‑1 (MQ=255)
                                                                       gTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                             >  2:3901909/1‑150 (MQ=255)
                                                                             aaaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAc                                                                       <  2:1834730/150‑1 (MQ=255)
                                                                              aaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                      >  2:3750166/1‑150 (MQ=255)
                                                                               acacCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCa                                                                     <  2:66276/150‑1 (MQ=255)
                                                                                        cTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAGTTGGTGGCCCTTGCTGGACTTGACCCAGCGACCAAg                                                            >  2:2658758/1‑150 (MQ=255)
                                                                                         tCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGc                                                           <  2:3422041/150‑1 (MQ=255)
                                                                                           aTTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGa                                                         >  2:2462306/1‑150 (MQ=255)
                                                                                            ttCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAt                                                        <  2:2107674/150‑1 (MQ=255)
                                                                                             tCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAtt                                                       >  1:2935315/1‑150 (MQ=255)
                                                                                               gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     >  1:3947072/1‑150 (MQ=255)
                                                                                               gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     <  1:38291/150‑1 (MQ=255)
                                                                                               gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     <  1:3705097/150‑1 (MQ=255)
                                                                                                aaaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATg                                                    <  2:43425/150‑1 (MQ=255)
                                                                                                   ccacccacCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGTGACCAAGCGATTATGAGt                                                 >  1:4326519/1‑150 (MQ=255)
                                                                                                     acccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                               <  1:10742/150‑1 (MQ=255)
                                                                                                     acccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                               >  1:3636671/1‑150 (MQ=255)
                                                                                                     acccaccGCACTTCGTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                               <  2:2393759/150‑1 (MQ=255)
                                                                                                        caccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCt                                            <  2:2774916/150‑1 (MQ=255)
                                                                                                         accGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTg                                           <  1:69628/150‑1 (MQ=255)
                                                                                                             cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       >  1:2279642/1‑150 (MQ=255)
                                                                                                             cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       >  2:3468751/1‑150 (MQ=255)
                                                                                                             cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       <  1:25015/150‑1 (MQ=255)
                                                                                                             cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       >  2:1365660/1‑150 (MQ=255)
                                                                                                             cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       <  1:3153792/150‑1 (MQ=255)
                                                                                                               cttcttCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTaa                                     >  2:913635/1‑150 (MQ=255)
                                                                                                                ttcttcCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAc                                    >  1:2056712/1‑150 (MQ=255)
                                                                                                                ttcttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAc                                    <  2:235290/150‑1 (MQ=255)
                                                                                                                ttcttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAc                                    >  1:2195401/1‑150 (MQ=255)
                                                                                                                 tcttcCTTGATATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATACTGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAcc                                   <  1:3582169/150‑1 (MQ=255)
                                                                                                                 tcttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAcc                                   >  2:2791666/1‑150 (MQ=255)
                                                                                                                         gAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGGACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCt                           <  1:1663868/150‑1 (MQ=255)
                                                                                                                           aaTGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTaa                         >  1:3284818/1‑150 (MQ=255)
                                                                                                                              ggCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAgg                      <  2:1769620/150‑1 (MQ=255)
                                                                                                                              ggCGGTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAgg                      >  1:2635841/1‑150 (MQ=255)
                                                                                                                                  ttAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCt                  <  1:1503810/150‑1 (MQ=255)
                                                                                                                                  ttAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCt                  <  2:4497539/150‑1 (MQ=255)
                                                                                                                                      tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAg              >  2:3055676/1‑150 (MQ=255)
                                                                                                                                      tCATGAAATATAGACAGACATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGat              <  2:2523846/150‑2 (MQ=255)
                                                                                                                                          gAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg          <  2:1173488/150‑1 (MQ=255)
                                                                                                                                          gAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg          <  2:2303891/150‑1 (MQ=255)
                                                                                                                                          gAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg          <  2:4172719/150‑1 (MQ=255)
                                                                                                                                           aaaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGc         >  2:1138549/1‑150 (MQ=255)
                                                                                                                                             atatAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCaa       >  2:2952871/1‑150 (MQ=255)
                                                                                                                                              tataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAt      >  2:1940168/1‑150 (MQ=255)
                                                                                                                                              tataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAt      >  1:2165455/1‑150 (MQ=255)
                                                                                                                                               ataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa     >  2:76709/1‑150 (MQ=255)
                                                                                                                                               ataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa     <  1:2882063/150‑1 (MQ=255)
                                                                                                                                               ataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa     >  1:161137/1‑150 (MQ=255)
                                                                                                                                                taGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATaa    <  2:1408704/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA  >  CP009273/2778918‑2779213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑