| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,878,668 | G→T | 85.2% | V637V (GTC→GTA) | ygcB ← | R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,878,668 | 0 | G | T | 85.2% | 646.3 / 130.2 | 279 | V637V (GTC→GTA) | ygcB | R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity |
| Reads supporting (aligned to +/- strand): ref base G (14/27); new base T (113/125); total (127/152) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01 | |||||||||||
Only 100 of 279 total aligned reads displayed.
AAAATGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAGCTCCT > CP009273/2878522‑2878816 | aTGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTgg > 2:3605740/1‑150 (MQ=255) aTGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTgg > 1:1919128/1‑150 (MQ=255) aTGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTgg > 2:3439406/1‑150 (MQ=255) aTGGTGGAAATAGGAATCTCAAACCCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTgg < 1:1135710/150‑1 (MQ=255) ggtggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATTTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGt < 2:3028733/150‑1 (MQ=255) gtggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGtt > 1:4000520/1‑150 (MQ=255) ggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGc > 2:2654469/1‑150 (MQ=255) gCAACAGGAATCTCAAAACCAGCGGGACTATTTTTGCGTTGATGGCTATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCg < 1:1984592/150‑1 (MQ=255) aaCAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGAc > 1:384741/1‑150 (MQ=255) aaCAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGAc > 1:1641945/1‑150 (MQ=255) aGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAg > 1:4052248/1‑150 (MQ=255) aTCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATc > 2:159874/1‑150 (MQ=255) aaaCCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCa > 1:364341/1‑150 (MQ=255) aaCCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCaa > 1:1656654/1‑150 (MQ=255) cAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACAt < 2:2436405/150‑1 (MQ=255) gCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACAttt < 2:851986/150‑1 (MQ=255) cGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTGCCTGGGTTGCGACAAGTATCCGTCCAACATTTc > 1:98034/1‑150 (MQ=255) ggACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGc > 1:4384308/1‑150 (MQ=255) gACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCt < 2:3009701/150‑1 (MQ=255) atatTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTccc > 1:1382166/1‑150 (MQ=255) gCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCAttttt < 2:141117/150‑1 (MQ=255) gCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAAtt < 1:1027705/150‑1 (MQ=255) gCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAAtt < 1:3836116/150‑1 (MQ=255) gCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAAtt < 1:1342898/150‑1 (MQ=255) cGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTg < 2:1714528/150‑1 (MQ=255) cGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTg < 2:1449829/150‑1 (MQ=255) gATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGc < 1:3681816/150‑1 (MQ=255) gATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGc > 1:520141/1‑150 (MQ=255) aaCGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCtaataa < 1:4526729/150‑1 (MQ=255) cGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACt > 1:2140518/1‑150 (MQ=255) ccAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGAt > 2:3548310/1‑150 (MQ=255) ccAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGAt > 1:196448/1‑150 (MQ=255) ccAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGAt < 1:3395909/150‑1 (MQ=255) tCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATtctc < 1:1011625/150‑1 (MQ=255) tCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATtctc < 1:717014/150‑1 (MQ=255) ttGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCttt < 2:599101/150‑1 (MQ=255) ggAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCttttt > 2:2411304/1‑150 (MQ=255) gAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTc > 1:3942100/1‑150 (MQ=255) aaaGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCac > 1:4141611/1‑150 (MQ=255) aaGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCacg > 1:2661233/1‑150 (MQ=255) aaGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCacg < 2:1921404/150‑1 (MQ=255) aGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTCATAACTCGATTCTCTTTTTCacga > 2:1157694/1‑150 (MQ=255) cAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCacgacg > 2:2720268/1‑150 (MQ=255) aaTCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGAt > 1:1533163/1‑150 (MQ=255) aTCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATc < 2:247326/150‑1 (MQ=255) aTCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATc < 2:2354628/150‑1 (MQ=255) aTCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATc > 2:3590915/1‑150 (MQ=255) tGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGtt > 2:2029203/1‑150 (MQ=255) tGCAGGACAATACTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATGCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGtt < 2:3127976/150‑1 (MQ=255) gCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTc < 2:1292639/150‑1 (MQ=255) cAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCa > 2:2516409/1‑150 (MQ=255) aGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAg > 1:3510555/1‑150 (MQ=255) aCAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGt < 1:443984/150‑1 (MQ=255) cAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTa < 2:1751837/150‑1 (MQ=255) tGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAg < 2:4417670/150‑1 (MQ=255) gCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgc < 2:3755907/150‑1 (MQ=255) cTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcg < 2:520141/150‑1 (MQ=255) cTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcg > 1:369251/1‑150 (MQ=255) cTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcg < 2:4018724/150‑1 (MQ=255) aGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcgcgc > 1:17735/1‑150 (MQ=255) aGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcgcgc > 1:4148184/1‑150 (MQ=255) aGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcgcgc > 1:1873846/1‑150 (MQ=255) aaTTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATg < 2:818332/150‑1 (MQ=255) aaTTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATg < 2:4344708/150‑1 (MQ=255) aaacactcataagcaaCGTCGAGGGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATACCTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAAc < 2:3738124/144‑1 (MQ=255) aaCCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAAc < 2:1068198/150‑1 (MQ=255) aaCCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAAc > 2:1992094/1‑150 (MQ=255) aaCCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAAc > 2:4231992/1‑150 (MQ=255) aCCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACa < 1:269485/150‑1 (MQ=255) aatcaaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAAt < 1:3603957/150‑1 (MQ=255) tcaaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCt < 1:2366332/150‑1 (MQ=255) caaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCta < 1:4251315/150‑1 (MQ=255) aaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCtat < 2:4138023/150‑1 (MQ=255) aaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTACAGCGCGCATGAAACAAATCtata > 2:1916054/1‑150 (MQ=255) aaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCtata < 1:200645/150‑1 (MQ=255) aatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTatat > 1:4384648/1‑150 (MQ=255) atcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATc < 1:3076467/150‑1 (MQ=255) atcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATc > 1:2949597/1‑150 (MQ=255) atcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATc < 2:1641945/150‑1 (MQ=255) atcaaCGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATc < 1:4245408/150‑1 (MQ=255) aaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTAc < 2:2074013/150‑1 (MQ=255) aaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTAc > 2:3686840/1‑150 (MQ=255) aaCGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTAc > 2:3795256/1‑150 (MQ=255) aCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACt < 1:93809/150‑1 (MQ=255) aCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACt < 1:3337012/150‑1 (MQ=255) aCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACt < 2:4000520/150‑1 (MQ=255) cGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACtt < 2:3305351/150‑1 (MQ=255) gTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTg > 1:688928/1‑150 (MQ=255) gTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTg < 1:589225/150‑1 (MQ=255) gTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTg > 1:3749715/1‑150 (MQ=255) gTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTg < 2:4448029/150‑1 (MQ=255) gATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTa > 2:4133784/1‑150 (MQ=255) aTTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTAt > 2:2363022/1‑150 (MQ=255) ttGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTAtt < 1:1807209/150‑1 (MQ=255) tGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTAttt < 1:1171406/150‑1 (MQ=255) tGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTAttt < 1:4482822/150‑1 (MQ=255) tGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTAttt < 1:2223527/150‑1 (MQ=255) ccACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAGCt < 1:2536546/150‑1 (MQ=255) ccACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAGCt < 2:135309/150‑1 (MQ=255) cGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAGCTCCt < 1:2848093/150‑1 (MQ=255) | AAAATGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAGCTCCT > CP009273/2878522‑2878816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |