Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,888,584 G→T 88.8% I185I (ATC→ATA)  ygcQ ← putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,888,5840GT88.8% 865.5 / 101.0 303I185I (ATC→ATA) ygcQputative flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (20/14);  new base T (139/129);  total (160/143)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 303 total aligned reads displayed.

TGCGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAT  >  CP009273/2888437‑2888730
                                                                                                                                                   |                                                                                                                                                  
tgcGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTAt                                                                                                                                                  <  1:515784/150‑1 (MQ=255)
       gAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTgg                                                                                                                                           <  1:3143093/150‑1 (MQ=255)
       gAAGCGCCAACAACAAGACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTgg                                                                                                                                           <  2:2891516/150‑1 (MQ=255)
        aaGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGt                                                                                                                                          <  2:3747220/150‑1 (MQ=255)
         aGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGtt                                                                                                                                         >  1:2881683/1‑150 (MQ=255)
          gcgcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTa                                                                                                                                        <  2:188484/150‑1 (MQ=255)
            gcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATc                                                                                                                                      >  2:4529922/1‑150 (MQ=255)
            gcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATc                                                                                                                                      >  2:4015769/1‑150 (MQ=255)
             ccAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCt                                                                                                                                     >  1:4511470/1‑150 (MQ=255)
               aacaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGc                                                                                                                                   <  1:3195956/150‑1 (MQ=255)
                acaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGcc                                                                                                                                  >  1:398467/1‑150 (MQ=255)
                acaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGcc                                                                                                                                  >  1:3826617/1‑150 (MQ=255)
                acaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGcc                                                                                                                                  >  2:1053915/1‑150 (MQ=255)
                acaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTGTTCTGCCAGCATAGCTATTTCCTGGTTATCTGcc                                                                                                                                  >  2:3334219/1‑150 (MQ=255)
                 caacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCct                                                                                                                                 <  2:3665718/150‑1 (MQ=255)
                  aacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCctc                                                                                                                                <  1:2335164/150‑1 (MQ=255)
                  aacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCctc                                                                                                                                <  1:66205/150‑1 (MQ=255)
                  aacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCctc                                                                                                                                >  2:4102877/1‑150 (MQ=255)
                   acaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCctct                                                                                                                               <  1:2621454/150‑1 (MQ=255)
                    caaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCtctc                                                                                                                              <  1:1050412/150‑1 (MQ=255)
                    caaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCtctc                                                                                                                              <  2:4170793/150‑1 (MQ=255)
                      aTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCg                                                                                                                            <  1:901312/150‑1 (MQ=255)
                      aTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCg                                                                                                                            <  1:899965/150‑1 (MQ=255)
                        acacacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGcc                                                                                                                          >  2:388311/1‑150 (MQ=255)
                          ccacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCt                                                                                                                        <  2:3227595/149‑1 (MQ=255)
                          acacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCt                                                                                                                        <  2:3283003/150‑1 (MQ=255)
                          acacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCt                                                                                                                        <  2:250138/150‑1 (MQ=255)
                             ccTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGcc                                                                                                                     >  1:3312975/1‑150 (MQ=255)
                              cTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGccc                                                                                                                    >  1:4265385/1‑150 (MQ=255)
                               tCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGcccc                                                                                                                   <  1:95814/150‑1 (MQ=255)
                                   gTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccacca                                                                                                               >  1:2975416/1‑150 (MQ=255)
                                    tGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTACTGGTTATCTGCCTCTCCGCCCTGCCccaccac                                                                                                              >  1:4217511/1‑150 (MQ=255)
                                       cagcagATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAg                                                                                                           >  2:343878/1‑150 (MQ=255)
                                        agcagATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGc                                                                                                          >  2:2668658/1‑150 (MQ=255)
                                            gATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAccc                                                                                                      >  1:1985624/1‑150 (MQ=255)
                                            gATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAccc                                                                                                      <  1:3044888/150‑1 (MQ=255)
                                             aTGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCCCCAGCACCCg                                                                                                     >  2:2403569/1‑150 (MQ=255)
                                             aTGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCg                                                                                                     >  2:1565827/1‑150 (MQ=255)
                                             aTGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCg                                                                                                     >  2:3173239/1‑150 (MQ=255)
                                             aTGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCg                                                                                                     >  2:3670902/1‑150 (MQ=255)
                                              tGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGt                                                                                                    >  1:572849/1‑150 (MQ=255)
                                                tCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCt                                                                                                  >  1:1394926/1‑150 (MQ=255)
                                                  cGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTgg                                                                                                <  1:754856/150‑1 (MQ=255)
                                                  cGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTgg                                                                                                <  2:791578/150‑1 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               <  1:833578/150‑1 (MQ=255)
                                                   ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCAATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               >  2:3821029/1‑150 (MQ=255)
                                                     aaaTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTCGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCt                                                                                             >  1:2854614/1‑150 (MQ=255)
                                                     aaaTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCt                                                                                             >  2:2739010/1‑150 (MQ=255)
                                                     aaaTACCAATCACTTGTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCt                                                                                             <  2:4177286/150‑1 (MQ=255)
                                                        tACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAg                                                                                          <  1:46117/150‑1 (MQ=255)
                                                        tACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAg                                                                                          >  1:2728488/1‑150 (MQ=255)
                                                           cAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTa                                                                                       <  1:2693813/150‑1 (MQ=255)
                                                            aaTCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTaa                                                                                      >  1:2163120/1‑150 (MQ=255)
                                                              tCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATg                                                                                    <  2:2355273/150‑1 (MQ=255)
                                                                 cTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTATCCCACTTCGGCTCCCACCTTGTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCTCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATgggg                                                                                 >  1:855601/1‑149 (MQ=255)
                                                                   ttttCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTcg                                                                               <  2:2048833/150‑1 (MQ=255)
                                                                     ttCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTcgcg                                                                             >  1:1706471/1‑150 (MQ=255)
                                                                       cAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCggg                                                                           >  1:2011521/1‑150 (MQ=255)
                                                                          cATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTaa                                                                        <  1:3865299/150‑1 (MQ=255)
                                                                          cATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTaa                                                                        <  2:1953416/150‑1 (MQ=255)
                                                                              aaCGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAtt                                                                    >  1:2230838/1‑150 (MQ=255)
                                                                               aCGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAttt                                                                   >  2:948382/1‑150 (MQ=255)
                                                                               aCGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAttt                                                                   >  2:1380050/1‑150 (MQ=255)
                                                                                  ccACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTc                                                                >  2:3078282/1‑150 (MQ=255)
                                                                                         tcatcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCct                                                         >  1:368898/1‑150 (MQ=255)
                                                                                          catcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctc                                                        <  2:3532128/150‑1 (MQ=255)
                                                                                           atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCTCCAGCACCCGTCTGGCCTCAGCTTATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       <  2:732723/150‑1 (MQ=255)
                                                                                           atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       <  2:3843048/150‑1 (MQ=255)
                                                                                            tcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtg                                                      >  2:4029533/1‑150 (MQ=255)
                                                                                             caCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtgt                                                     >  2:3916125/1‑150 (MQ=255)
                                                                                              aCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtgtg                                                    <  2:2221005/150‑1 (MQ=255)
                                                                                              aCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCtgtg                                                    >  2:1271313/1‑150 (MQ=255)
                                                                                                 cgcgCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTa                                                 <  2:1234843/150‑1 (MQ=255)
                                                                                                 cgcgCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTa                                                 <  2:3575465/150‑1 (MQ=255)
                                                                                                   cgcCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAc                                               <  2:1450604/150‑1 (MQ=255)
                                                                                                     cccGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAcca                                             <  2:256164/150‑1 (MQ=255)
                                                                                                      ccGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccag                                            >  2:3799553/1‑150 (MQ=255)
                                                                                                      ccGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccag                                            <  2:3962317/150‑1 (MQ=255)
                                                                                                       cGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagc                                           >  1:3508045/1‑150 (MQ=255)
                                                                                                          cTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagccag                                        <  1:4330067/150‑1 (MQ=255)
                                                                                                             tAGCACACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTcc                                     <  1:4297232/150‑1 (MQ=255)
                                                                                                                cccACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGa                                  >  2:541899/1‑150 (MQ=255)
                                                                                                                   aCTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAgc                               >  2:4336000/1‑150 (MQ=255)
                                                                                                                              cAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGAc                    >  1:194347/1‑150 (MQ=255)
                                                                                                                                 cTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAAt                 >  2:65159/1‑150 (MQ=255)
                                                                                                                                 cTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAAt                 >  2:3094277/1‑150 (MQ=255)
                                                                                                                                   tttCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGt               <  1:4116194/150‑1 (MQ=255)
                                                                                                                                    ttCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGtt              <  1:3104501/150‑1 (MQ=255)
                                                                                                                                     tCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTc             <  1:1876880/150‑1 (MQ=255)
                                                                                                                                      cTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCa            <  1:1631156/150‑1 (MQ=255)
                                                                                                                                        gCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgt          >  1:1572647/1‑150 (MQ=255)
                                                                                                                                          cAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgttg        >  2:3712607/1‑150 (MQ=255)
                                                                                                                                           aGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgttgt       >  2:867992/1‑150 (MQ=255)
                                                                                                                                           aGCATAGCTATTTACTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgttgt       <  2:4217511/150‑1 (MQ=255)
                                                                                                                                             cATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGttgttg     >  1:4375344/1‑150 (MQ=255)
                                                                                                                                              aTAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGc    <  1:3079890/150‑1 (MQ=255)
                                                                                                                                               tAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCa   >  2:1752195/1‑150 (MQ=255)
                                                                                                                                                aGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAt  <  2:1690656/150‑1 (MQ=255)
                                                                                                                                                aGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAt  <  1:4055848/150‑1 (MQ=255)
                                                                                                                                                aGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAt  <  1:1543253/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAT  >  CP009273/2888437‑2888730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑