BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,923,478	C→A	5.5%	Q182K (CAG→AAG)	sdaB →	L‑serine dehydratase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,923,478	0	C	A	5.5%	1109.5 / 30.2	289	Q182K (CAG→AAG)	sdaB	L‑serine dehydratase 2
Reads supporting (aligned to +/- strand): ref base C (138/135); new base A (6/10); total (144/145)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 291 total aligned reads displayed.

GGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCT  >  CP009273/2923330‑2923627

  cgggcgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAgg                                                                                                                                                    <  1:3129812/150‑1 (MQ=255)

  cgggcgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAgg                                                                                                                                                    <  1:256518/150‑1 (MQ=255)

  cgggcgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAgg                                                                                                                                                    <  1:3693812/150‑1 (MQ=255)

  cgggcgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAgg                                                                                                                                                    <  1:2221265/150‑1 (MQ=255)

   gggcgATAAAGTCGTGTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGa                                                                                                                                                   <  2:4401466/150‑1 (MQ=255)

    ggcgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTACTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGaa                                                                                                                                                  >  2:4130135/1‑150 (MQ=255)

      cgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAAc                                                                                                                                                <  2:2471287/150‑1 (MQ=255)

         tAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTAAGGAAACCgg                                                                                                                                             <  1:2809107/150‑1 (MQ=255)

          aaaGTCGTTTACAGCCAGACTTACAACACTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCCGTTGTAGTTCCATATCCGTACAGTTCAGCAGCCGATCTGCAAAAAAAATGTAAGGAAACCggg                                                                                                                                            >  2:1613080/1‑150 (MQ=255)

           aaGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGc                                                                                                                                           >  2:3597275/1‑150 (MQ=255)

            aGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCACAAACATTGTCAGGAAACCGGGCt                                                                                                                                          >  2:1709356/1‑150 (MQ=255)

             gTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTg                                                                                                                                         >  2:2825684/1‑150 (MQ=255)

               cGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTc                                                                                                                                       <  1:412559/150‑1 (MQ=255)

                gTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCa                                                                                                                                      <  1:4197470/150‑1 (MQ=255)

                gTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCa                                                                                                                                      <  1:1978878/150‑1 (MQ=255)

                     cAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCActctc                                                                                                                                 <  2:2641839/150‑1 (MQ=255)

                      aGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCActctct                                                                                                                                >  2:341008/1‑150 (MQ=255)

                      aGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCActctct                                                                                                                                <  1:1667696/150‑1 (MQ=255)

                        ccAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTgg                                                                                                                              >  1:2010605/1‑150 (MQ=255)

                        ccAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTgg                                                                                                                              >  2:2400458/1‑150 (MQ=255)

                          aGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGcc                                                                                                                            >  1:1974807/1‑150 (MQ=255)

                           gACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCt                                                                                                                           <  2:2275634/150‑1 (MQ=255)

                           gACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGGCAGGAAACCGGGCTGTCACTCTCTGGCCt                                                                                                                           <  2:2021387/150‑1 (MQ=255)

                           gACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGGTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCt                                                                                                                           <  1:3262476/150‑1 (MQ=255)

                             cTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCtga                                                                                                                         >  2:3724424/1‑150 (MQ=255)

                              ttACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCtgat                                                                                                                        <  1:1750789/150‑1 (MQ=255)

                                  tactCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCtgatgatg                                                                                                                    >  1:1062638/1‑150 (MQ=255)

                                  tactCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCtgatgatg                                                                                                                    >  2:1473958/1‑150 (MQ=255)

                                   actCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCtgatgatga                                                                                                                   >  1:3228744/1‑150 (MQ=255)

                                    ctctATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGaa                                                                                                                  >  2:4012950/1‑150 (MQ=255)

                                        aTTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAAc                                                                                                              >  2:3842429/1‑150 (MQ=255)

                                          tGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGa                                                                                                            >  1:2890516/1‑150 (MQ=255)

                                           ggCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAg                                                                                                           <  1:454529/150‑1 (MQ=255)

                                            gCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGc                                                                                                          <  1:2101908/150‑1 (MQ=255)

                                                tGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGgc                                                                                                      >  2:4478047/1‑150 (MQ=255)

                                                tGGATTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGgc                                                                                                      <  2:1567669/150‑1 (MQ=255)

                                                   cTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCt                                                                                                   <  2:3497322/150‑1 (MQ=255)

                                                   cTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCt                                                                                                   <  2:1194842/150‑1 (MQ=255)

                                                     ttATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGc                                                                                                 >  1:4344012/1‑150 (MQ=255)

                                                     ttATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGc                                                                                                 >  1:8130/1‑150 (MQ=255)

                                                      tATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGca                                                                                                >  1:1318131/1‑150 (MQ=255)

                                                       aTCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGcac                                                                                               >  2:3352453/1‑150 (MQ=255)

                                                            tgatgaAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCaa                                                                                          >  2:3264284/1‑150 (MQ=255)

                                                               tgaAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAaaga                                                                                       <  1:1881920/150‑1 (MQ=255)

                                                               tgaAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAaaga                                                                                       >  1:2180134/1‑150 (MQ=255)

                                                                 aaGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAaagaag                                                                                     >  1:898828/1‑150 (MQ=255)

                                                                  agagCATTTTGGCCAGCAGGATAGCGCATCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAagaaga                                                                                    >  2:4042199/1‑150 (MQ=255)

                                                                     gCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCt                                                                                 <  1:64541/150‑1 (MQ=255)

                                                                      cATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTg                                                                                >  1:2416277/1‑150 (MQ=255)

                                                                           tGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACa                                                                           >  1:185198/1‑150 (MQ=255)

                                                                            ggCCAGCAGGATAGCGCACCGGGTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAg                                                                          >  2:1539276/1‑150 (MQ=255)

                                                                             gCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTAAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGc                                                                         <  2:3124025/150‑1 (MQ=255)

                                                                               cagcagGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCAc                                                                       >  2:3388691/1‑150 (MQ=255)

                                                                               cagcagGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCAc                                                                       >  2:1253680/1‑150 (MQ=255)

                                                                                     gATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCg                                                                 >  2:2115915/1‑150 (MQ=255)

                                                                                     gATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATATGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCg                                                                 <  1:751432/150‑1 (MQ=255)

                                                                                       tAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGaa                                                               >  2:465796/1‑150 (MQ=255)

                                                                                        aGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAAc                                                              >  2:602845/1‑150 (MQ=255)

                                                                                        aGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAAc                                                              >  1:1154295/1‑150 (MQ=255)

                                                                                        aGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAAc                                                              <  1:3810709/150‑1 (MQ=255)

                                                                                        aGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAAc                                                              <  2:1296831/150‑1 (MQ=255)

                                                                                          cgcACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGt                                                            >  1:3448847/1‑150 (MQ=255)

                                                                                           gcACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTc                                                           >  2:1922938/1‑150 (MQ=255)

                                                                                            cACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCt                                                          <  2:2702494/150‑1 (MQ=255)

                                                                                            cACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTAATGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCt                                                          <  2:4303823/150‑1 (MQ=255)

                                                                                              ccGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTgg                                                        <  1:30214/150‑1 (MQ=255)

                                                                                              ccGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTgg                                                        >  1:4098897/1‑150 (MQ=255)

                                                                                                 gTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGaa                                                     <  2:4339190/150‑1 (MQ=255)

                                                                                                      aGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCAt                                                >  2:2409636/1‑150 (MQ=255)

                                                                                                        ttCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGTAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATgc                                              <  2:2764056/150‑1 (MQ=255)

                                                                                                           cTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGcgcg                                           >  1:2915267/1‑150 (MQ=255)

                                                                                                            ttATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCgcgg                                          <  2:87083/150‑1 (MQ=255)

                                                                                                                 cGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTa                                     >  2:3056743/1‑150 (MQ=255)

                                                                                                                  gTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTAt                                    >  1:3888843/1‑150 (MQ=255)

                                                                                                                   tACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTAtt                                   <  1:510674/150‑1 (MQ=255)

                                                                                                                   tACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTAtt                                   >  1:2054249/1‑150 (MQ=255)

                                                                                                                     cAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGa                                 <  2:1784323/150‑1 (MQ=255)

                                                                                                                      aGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAg                                <  1:1286776/150‑1 (MQ=255)

                                                                                                                        ttCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAgcg                              <  1:4042199/150‑1 (MQ=255)

                                                                                                                        ttCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAgcg                              <  1:351145/150‑1 (MQ=255)

                                                                                                                              agcCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTAt                        >  1:1670251/1‑150 (MQ=255)

                                                                                                                               gcCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTAtt                       <  2:2418566/150‑1 (MQ=255)

                                                                                                                                ccGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTAttt                      >  2:2376528/1‑150 (MQ=255)

                                                                                                                                ccGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTAttt                      >  1:3405231/1‑150 (MQ=255)

                                                                                                                                  gATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTcc                    >  2:1722352/1‑150 (MQ=255)

                                                                                                                                   aTCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCa                   >  1:4228532/1‑150 (MQ=255)

                                                                                                                                   aTCTGCAAAAACATTGTAAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCa                   <  2:945714/150‑1 (MQ=255)

                                                                                                                                      tGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCg                <  2:699824/150‑1 (MQ=255)

                                                                                                                                      tGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCg                >  2:1449842/1‑150 (MQ=255)

                                                                                                                                       gCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGa               >  2:4192885/1‑150 (MQ=255)

                                                                                                                                         aaaaaCATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAg             >  2:4539368/1‑150 (MQ=255)

                                                                                                                                           aaaCATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCGGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGc           <  1:2855784/150‑1 (MQ=255)

                                                                                                                                            aaCATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCg          >  1:4451479/1‑150 (MQ=255)

                                                                                                                                             aCATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCgt         <  2:816369/150‑1 (MQ=255)

                                                                                                                                              cATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCgtg        <  1:2636961/150‑1 (MQ=255)

                                                                                                                                                ttGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGtt      <  2:1039318/150‑1 (MQ=255)

                                                                                                                                                 tGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTg     <  2:2895865/150‑1 (MQ=255)

                                                                                                                                                 tGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTg     >  2:1108214/1‑150 (MQ=255)

                                                                                                                                                 tGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTg     <  2:744104/150‑1 (MQ=255)

                                                                                                                                                    cAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCt  >  1:2471797/1‑150 (MQ=255)

GGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTCACTCTCTGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCT  >  CP009273/2923330‑2923627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`