Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,967,572 A→G 6.9% F601S (TTT→TCT)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,967,5720AG 6.9% 1020.2 / 47.4 302F601S (TTT→TCT) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base A (141/139);  new base G (8/13);  total (149/153)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 309 total aligned reads displayed.

TGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCT  >  CP009273/2967423‑2967721
                                                                                                                                                     |                                                                                                                                                     
     tCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGc                                                                                                                                                  >  1:417915/1‑150 (MQ=255)
       gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGcac                                                                                                                                                <  2:3741911/150‑1 (MQ=255)
        ctctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGcaca                                                                                                                                               <  2:2633927/150‑1 (MQ=255)
        ctctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGcaca                                                                                                                                               >  1:240525/1‑150 (MQ=255)
         tctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACaa                                                                                                                                              <  1:1058013/150‑1 (MQ=255)
          ctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAat                                                                                                                                             <  2:3809157/150‑1 (MQ=255)
          ctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAat                                                                                                                                             >  1:1835822/1‑150 (MQ=255)
          ctTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAat                                                                                                                                             <  1:2767130/150‑1 (MQ=255)
                   aGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAgt                                                                                                                                    >  2:1268997/1‑150 (MQ=255)
                        cATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAt                                                                                                                               <  1:1790871/150‑1 (MQ=255)
                        cATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAt                                                                                                                               <  2:4199090/150‑1 (MQ=255)
                            gACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCa                                                                                                                           <  2:3200091/150‑1 (MQ=255)
                            gACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCa                                                                                                                           >  1:3728286/1‑150 (MQ=255)
                                    cTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCt                                                                                                                   >  2:2705876/1‑150 (MQ=255)
                                    cTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCt                                                                                                                   >  2:3434859/1‑150 (MQ=255)
                                      ggCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTcc                                                                                                                 >  1:3594370/1‑150 (MQ=255)
                                        cGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAt                                                                                                               >  2:2825162/1‑150 (MQ=255)
                                        cGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAt                                                                                                               >  2:210260/1‑150 (MQ=255)
                                        cGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAt                                                                                                               >  2:4114998/1‑150 (MQ=255)
                                          aaaCACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAttt                                                                                                             <  2:3455334/150‑1 (MQ=255)
                                            acacCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcg                                                                                                           <  1:1379145/150‑1 (MQ=255)
                                            acacCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcg                                                                                                           >  1:2577551/1‑150 (MQ=255)
                                              acCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgcc                                                                                                         >  2:3011750/1‑150 (MQ=255)
                                               ccAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCAGTTcgccg                                                                                                        >  2:1426917/1‑150 (MQ=255)
                                                cAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTcgccgc                                                                                                       >  1:3981429/1‑150 (MQ=255)
                                                 aaGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCcgcg                                                                                                      <  1:3737368/150‑1 (MQ=255)
                                                   gTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGaa                                                                                                    <  1:702369/150‑1 (MQ=255)
                                                      ccAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACAt                                                                                                 >  2:4427230/1‑150 (MQ=255)
                                                       cAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACAtt                                                                                                >  2:3662276/1‑150 (MQ=255)
                                                       cAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACAtt                                                                                                >  1:892218/1‑150 (MQ=255)
                                                        aGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATtc                                                                                               >  2:3024585/1‑150 (MQ=255)
                                                          ttgttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATtctc                                                                                             >  1:1984655/1‑150 (MQ=255)
                                                           tgttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcg                                                                                            >  1:4328314/1‑150 (MQ=255)
                                                             ttCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTcggc                                                                                          <  1:2193032/150‑1 (MQ=255)
                                                                caccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGt                                                                                       <  1:298360/150‑1 (MQ=255)
                                                                caccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGt                                                                                       <  2:4061070/150‑1 (MQ=255)
                                                                caccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGt                                                                                       <  1:2564292/150‑1 (MQ=255)
                                                                 accaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTg                                                                                      <  1:4540764/150‑1 (MQ=255)
                                                                  ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTgg                                                                                     >  2:723517/1‑150 (MQ=255)
                                                                  ccaTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTgg                                                                                     >  2:3172821/1‑150 (MQ=255)
                                                                   caTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTggg                                                                                    <  1:521399/150‑1 (MQ=255)
                                                                    aTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGc                                                                                   <  2:4226854/150‑1 (MQ=255)
                                                                    aTTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCTATTTCGCCGCGAACATTCTCGGCGGTGGGc                                                                                   >  2:173631/1‑150 (MQ=255)
                                                                       tCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACt                                                                                >  1:3336234/1‑150 (MQ=255)
                                                                          aGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTcc                                                                             >  2:1058428/1‑150 (MQ=255)
                                                                             gACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGt                                                                          >  1:1184801/1‑150 (MQ=255)
                                                                             gACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGt                                                                          <  1:3297964/150‑1 (MQ=255)
                                                                              acacCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTa                                                                         <  1:3082098/150‑1 (MQ=255)
                                                                                  cATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCTCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTCCAcc                                                                     >  1:1742583/1‑150 (MQ=255)
                                                                                  cATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACAcc                                                                     >  2:4170240/1‑150 (MQ=255)
                                                                                    tttCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTg                                                                   <  1:2829031/150‑1 (MQ=255)
                                                                                     ttCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTgg                                                                  <  1:1919366/150‑1 (MQ=255)
                                                                                      tCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGc                                                                 >  2:3968310/1‑150 (MQ=255)
                                                                                       cGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCt                                                                >  2:4411802/1‑150 (MQ=255)
                                                                                        gCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCtt                                                               <  1:755267/150‑1 (MQ=255)
                                                                                         ccTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtc                                                              >  1:4008797/1‑150 (MQ=255)
                                                                                          cTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtct                                                             >  1:3232890/1‑150 (MQ=255)
                                                                                          cTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTtct                                                             >  1:1933267/1‑150 (MQ=255)
                                                                                            gCAATTTTGGCAATGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTcc                                                           <  1:3414157/150‑1 (MQ=255)
                                                                                                 tttGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCg                                                      <  2:2244746/150‑1 (MQ=255)
                                                                                                   tGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCa                                                    >  2:2367988/1‑150 (MQ=255)
                                                                                                    ggCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAg                                                   >  2:2547365/1‑150 (MQ=255)
                                                                                                    ggCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAg                                                   >  2:3753574/1‑150 (MQ=255)
                                                                                                      cAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGAt                                                 >  2:4488807/1‑150 (MQ=255)
                                                                                                        aaGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAg                                               >  2:550529/1‑150 (MQ=255)
                                                                                                           cGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTCCACCTGGCTTCTCCACCCGCAGATAGCCg                                            >  2:2955928/1‑150 (MQ=255)
                                                                                                           cGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCg                                            <  1:1101917/150‑1 (MQ=255)
                                                                                                               ttGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCa                                        <  1:807937/150‑1 (MQ=255)
                                                                                                                 gcgcGGCCCTGAATCTGCACAAAGCCCTGCTCGTCACAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCAtt                                      <  2:3926000/150‑1 (MQ=255)
                                                                                                                   gcgGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTAt                                    >  1:2348498/1‑150 (MQ=255)
                                                                                                                      gCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGtt                                 <  1:2870496/150‑1 (MQ=255)
                                                                                                                       cccTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTc                                <  1:1341904/150‑1 (MQ=255)
                                                                                                                       cccTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTc                                <  2:1560963/150‑1 (MQ=255)
                                                                                                                        ccTGAATCTGCACAAAGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCg                               <  2:3479336/150‑1 (MQ=255)
                                                                                                                        ccTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCg                               >  1:2437300/1‑150 (MQ=255)
                                                                                                                        ccTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCg                               <  1:3811877/150‑1 (MQ=255)
                                                                                                                         cTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCgg                              <  1:1268997/150‑1 (MQ=255)
                                                                                                                           gAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGcc                            >  1:1510167/1‑150 (MQ=255)
                                                                                                                             aTCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCt                          >  2:2237210/1‑150 (MQ=255)
                                                                                                                             aTCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCt                          <  2:4449797/150‑1 (MQ=255)
                                                                                                                             aTCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCt                          >  2:3570801/1‑150 (MQ=255)
                                                                                                                              tCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCtt                         <  1:592247/150‑1 (MQ=255)
                                                                                                                              tCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCtt                         <  1:2527614/150‑1 (MQ=255)
                                                                                                                               cTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCttt                        <  2:1985684/150‑1 (MQ=255)
                                                                                                                                  cacaAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAg                     <  2:1552917/150‑1 (MQ=255)
                                                                                                                                   acaAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGt                    <  1:721816/150‑1 (MQ=255)
                                                                                                                                   acaAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGt                    >  2:4361457/1‑150 (MQ=255)
                                                                                                                                     aaaGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTg                  >  1:2639798/1‑150 (MQ=255)
                                                                                                                                      aaGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGc                 >  1:1667306/1‑150 (MQ=255)
                                                                                                                                       aGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCa                <  2:1654433/150‑1 (MQ=255)
                                                                                                                                       aGCCCTGCTCGTCAGAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCa                <  2:1928818/150‑1 (MQ=255)
                                                                                                                                       aGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCa                <  1:2613162/150‑1 (MQ=255)
                                                                                                                                        gCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAg               <  2:827947/150‑1 (MQ=255)
                                                                                                                                         cccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAgg              <  1:1706842/150‑1 (MQ=255)
                                                                                                                                         cccTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAgg              <  1:4264563/150‑1 (MQ=255)
                                                                                                                                           cTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCg            >  2:2449566/1‑150 (MQ=255)
                                                                                                                                               tCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCg        <  2:849371/150‑1 (MQ=255)
                                                                                                                                               tCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCg        <  2:4399761/150‑1 (MQ=255)
                                                                                                                                                 gTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGcc      <  2:495436/150‑1 (MQ=255)
                                                                                                                                                    aaaaCGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCctc   <  2:2990373/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGCGACACCATTTCGCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCT  >  CP009273/2967423‑2967721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑