Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,014,214 G→T 13.7% E106* (GAA→TAA)  ygfM → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,014,2140GT13.7% 857.6 / 80.2 262E106* (GAA→TAA) ygfMputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (105/120);  new base T (17/20);  total (122/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAA  >  CP009273/3014065‑3014363
                                                                                                                                                     |                                                                                                                                                     
  gTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTaa                                                                                                                                                     <  2:181417/150‑1 (MQ=255)
   ttGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGaaa                                                                                                                                                    <  2:1898805/150‑1 (MQ=255)
    tGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAAt                                                                                                                                                   <  1:2803835/150‑1 (MQ=255)
    tGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAAt                                                                                                                                                   <  2:1408957/150‑1 (MQ=255)
     gACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATc                                                                                                                                                  <  1:2408091/150‑1 (MQ=255)
      aCTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcg                                                                                                                                                 >  2:1288350/1‑150 (MQ=255)
       cTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcgc                                                                                                                                                >  2:1097894/1‑150 (MQ=255)
        tGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATcgcc                                                                                                                                               >  1:1361403/1‑150 (MQ=255)
        tGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcgcc                                                                                                                                               >  1:3449396/1‑150 (MQ=255)
              aaCGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGccgcccgc                                                                                                                                         >  1:3468900/1‑150 (MQ=255)
                cGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCAGGTTCGTAATCAGTCGACCAGTGGTGGTTAAATCGCCGCCCGCCa                                                                                                                                       >  2:3433984/1‑150 (MQ=255)
                 ggTGAACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAg                                                                                                                                      <  1:3841447/150‑1 (MQ=255)
                 ggTGAACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAg                                                                                                                                      <  1:894648/150‑1 (MQ=255)
                   tGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGa                                                                                                                                    <  2:2096024/150‑1 (MQ=255)
                     cACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAag                                                                                                                                  <  1:3791444/150‑1 (MQ=255)
                       cTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAagag                                                                                                                                >  2:4449212/1‑150 (MQ=255)
                         gCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTc                                                                                                                              <  1:1249692/150‑1 (MQ=255)
                          cGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCg                                                                                                                             <  1:1288745/150‑1 (MQ=255)
                            gATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGt                                                                                                                           >  1:3239793/1‑150 (MQ=255)
                            gATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGt                                                                                                                           <  2:1251066/150‑1 (MQ=255)
                             aTTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtg                                                                                                                          <  2:4145704/150‑1 (MQ=255)
                              ttGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtgc                                                                                                                         >  2:2700986/1‑150 (MQ=255)
                              ttGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtgc                                                                                                                         >  2:904199/1‑150 (MQ=255)
                               tGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtgct                                                                                                                        >  2:875368/1‑150 (MQ=255)
                               tGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtgct                                                                                                                        >  2:1042997/1‑150 (MQ=255)
                               tGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtgct                                                                                                                        <  1:2269306/150‑1 (MQ=255)
                               tGGAGCTATGGCTCGCTTGCAGCCAATGCGTGATGCTCGATTTATTCCTGCAGCGATGCTTGTAGCCCTCGGTTTTGTTTACTCACGCCATGTTCTTAATCAGTCGACCATTGTTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGtgct                                                                                                                        <  1:21974/150‑1 (MQ=255)
                                    cAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTc                                                                                                                   >  1:4082450/1‑150 (MQ=255)
                                     aaTGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTcc                                                                                                                  >  1:1928855/1‑150 (MQ=255)
                                     aaTGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTcc                                                                                                                  >  2:4267948/1‑150 (MQ=255)
                                      aTGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTccc                                                                                                                 <  1:1463547/150‑1 (MQ=255)
                                      aTGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTccc                                                                                                                 <  1:3032380/150‑1 (MQ=255)
                                       tGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCg                                                                                                                >  1:1604192/1‑150 (MQ=255)
                                        gTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGGGCTGCTTCCCGt                                                                                                               >  1:4292185/1‑150 (MQ=255)
                                        gTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCAGTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGt                                                                                                               >  1:4061447/1‑150 (MQ=255)
                                         tctcGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTc                                                                                                              <  1:2980978/150‑1 (MQ=255)
                                          ctcGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTcc                                                                                                             >  1:1075507/1‑150 (MQ=255)
                                           tcGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCt                                                                                                            <  1:2123840/150‑1 (MQ=255)
                                                   aGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCAc                                                                                                    >  1:2996929/1‑150 (MQ=255)
                                                    gCCTCTGCGTGTTTCTCGATTTATTCCTGCAGCTATGCGTTAAGCCATTGTTTTTGTTTACTCAAGCAATGTTCGTAATCAGTCGACCATTGGTGGTGAATTCGACGCCCGCCAGGAAGTGTCGGTGCTTCTTGCCTTCCTGTTGGctat                                                                                                   <  2:3774886/150‑4 (MQ=255)
                                                    gCCACGGCGTGATGCGCGAATTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCAGGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACt                                                                                                   <  2:2584685/150‑1 (MQ=255)
                                                     ccACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTg                                                                                                  >  1:2430813/1‑150 (MQ=255)
                                                      cACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTgg                                                                                                 >  1:3561601/1‑150 (MQ=255)
                                                        cTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGAt                                                                                               <  2:2965866/150‑1 (MQ=255)
                                                        cTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGAt                                                                                               <  2:4079347/150‑1 (MQ=255)
                                                           cGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCt                                                                                            >  1:229776/1‑150 (MQ=255)
                                                            gTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTg                                                                                           <  1:1572992/150‑1 (MQ=255)
                                                            gTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTGTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTg                                                                                           <  1:593657/150‑1 (MQ=255)
                                                              gATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGaa                                                                                         <  2:1655559/150‑1 (MQ=255)
                                                              gATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGaa                                                                                         >  2:310311/1‑150 (MQ=255)
                                                                  cgcgATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTgg                                                                                     <  2:2753602/150‑1 (MQ=255)
                                                                       tttATTCCTGCAGCGCTGCGTGAAGCCCTAGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGTTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGttttt                                                                                <  2:4160170/150‑1 (MQ=255)
                                                                        ttATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTg                                                                               <  2:3233487/150‑1 (MQ=255)
                                                                         tATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTgg                                                                              <  1:467608/150‑1 (MQ=255)
                                                                           ttCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCa                                                                            <  1:4145677/150‑1 (MQ=255)
                                                                             ccTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTGACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAAc                                                                          <  2:3638065/150‑1 (MQ=255)
                                                                              cTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACg                                                                         <  1:1101265/150‑1 (MQ=255)
                                                                              cTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACg                                                                         <  2:3971076/150‑1 (MQ=255)
                                                                               tGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACgg                                                                        <  1:1186932/150‑1 (MQ=255)
                                                                               tGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACgg                                                                        <  2:1181653/150‑1 (MQ=255)
                                                                                 cAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCg                                                                      <  1:2576409/150‑1 (MQ=255)
                                                                                   gcgcTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGaa                                                                    <  2:2435293/150‑1 (MQ=255)
                                                                                        gCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCt                                                               <  2:759085/150‑1 (MQ=255)
                                                                                          gTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGt                                                             <  1:2693035/150‑1 (MQ=255)
                                                                                            gAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCa                                                           >  2:4342643/1‑150 (MQ=255)
                                                                                                 ccTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGa                                                      >  1:487898/1‑150 (MQ=255)
                                                                                                  cTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAg                                                     >  2:1134017/1‑150 (MQ=255)
                                                                                                  cTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAg                                                     <  1:1500343/150‑1 (MQ=255)
                                                                                                  cTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAg                                                     <  1:389012/150‑1 (MQ=255)
                                                                                                     ggTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGAc                                                  >  1:3707611/1‑150 (MQ=255)
                                                                                                          tGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCt                                             >  1:1034478/1‑150 (MQ=255)
                                                                                                          tGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCt                                             >  1:4016691/1‑150 (MQ=255)
                                                                                                            tttACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTgg                                           <  2:3287879/150‑1 (MQ=255)
                                                                                                            tttACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTgg                                           <  2:1009206/150‑1 (MQ=255)
                                                                                                              tACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGcc                                         >  1:3508029/1‑150 (MQ=255)
                                                                                                              tACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGcc                                         >  1:3292949/1‑150 (MQ=255)
                                                                                                                  cACGCCATGTTCGTAATCAGTCGACCATTGGTGGTTAACTCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGcc                                     <  1:3117474/150‑1 (MQ=255)
                                                                                                                  cACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGcc                                     <  1:1745605/150‑1 (MQ=255)
                                                                                                                  cACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGcc                                     <  1:1794770/150‑1 (MQ=255)
                                                                                                                       cATGTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGc                                <  2:4380075/150‑1 (MQ=255)
                                                                                                                       cATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCCATCGAGGACTACCTGGCCTGCCCATGc                                >  1:730989/1‑150 (MQ=255)
                                                                                                                       cATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGc                                <  2:3471965/150‑1 (MQ=255)
                                                                                                                       cATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGc                                <  2:2906479/150‑1 (MQ=255)
                                                                                                                          gTTCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGAt                             <  2:623263/150‑1 (MQ=255)
                                                                                                                          gTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGAt                             >  1:3051791/1‑150 (MQ=255)
                                                                                                                           ttCGTAATCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATc                            <  1:2445802/150‑1 (MQ=255)
                                                                                                                           ttCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATc                            <  1:4492634/150‑1 (MQ=255)
                                                                                                                               tAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCt                        >  2:1364650/1‑150 (MQ=255)
                                                                                                                                  tCAGTCGACCATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATAGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCt                     >  1:2036284/1‑150 (MQ=255)
                                                                                                                                  tCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCt                     <  2:3113943/150‑1 (MQ=255)
                                                                                                                                  tCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCt                     <  2:1305788/150‑1 (MQ=255)
                                                                                                                                  tCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCAGCCCATGCGATCGCCTGCt                     >  1:1161747/1‑150 (MQ=255)
                                                                                                                                    aGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTaa                   >  1:1152465/1‑150 (MQ=255)
                                                                                                                                    aGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTaa                   >  2:2399735/1‑150 (MQ=255)
                                                                                                                                      tCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAAcc                 <  2:3042475/150‑1 (MQ=255)
                                                                                                                                       cGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCg                >  2:3833356/1‑150 (MQ=255)
                                                                                                                                           cATTGGTGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAAt            >  1:1845891/1‑150 (MQ=255)
                                                                                                                                            aTTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCAGTACTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTAAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAAtt           <  1:3397037/150‑1 (MQ=255)
                                                                                                                                             ttGGGGGTTAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCGTCCCGGCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAAGTa          <  2:3181182/150‑1 (MQ=255)
                                                                                                                                                    tgAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTaa   >  2:1551417/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAACGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAA  >  CP009273/3014065‑3014363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑