Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 283,636 A→C 6.1% Q379H (CAA→CAC)  yagH → CP4‑6 prophage; putative xylosidase/arabinosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273283,6360AC 6.1% 677.1 / 7.7 262Q379H (CAA→CAC) yagHCP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (153/86);  new base C (2/21);  total (155/107)
Fisher's exact test for biased strand distribution p-value = 2.08e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

AACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGG  >  CP009273/283487‑283785
                                                                                                                                                     |                                                                                                                                                     
aaCTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCaa                                                                                                                                                       >  1:3780188/1‑150 (MQ=255)
 aCTGGCGTGAAGATTTCGACGCCATTTCGCTTGAACAGGAGCTGCAGACCCTGCGCATTCAGTTCGACGACACCCTCGGCTCGCTAACCGCGCGCCCGGGCTTCTAACGGATCTATGTCAACGACTCGATCAATTCGAACAACACCCAAt                                                                                                                                                      >  2:2031757/1‑150 (MQ=255)
  cTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATc                                                                                                                                                     >  2:605657/1‑150 (MQ=255)
  cTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATc                                                                                                                                                     <  1:2628484/150‑1 (MQ=255)
  cTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATc                                                                                                                                                     >  1:837656/1‑150 (MQ=255)
   tGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCTCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCg                                                                                                                                                    <  2:2061943/150‑1 (MQ=255)
      cGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGAcc                                                                                                                                                 <  2:1068221/150‑1 (MQ=255)
               ttCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGgcgcgc                                                                                                                                        >  1:1472341/1‑150 (MQ=255)
                 cGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCACCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGcgccg                                                                                                                                      >  2:879936/1‑150 (MQ=255)
                 cGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGcgccg                                                                                                                                      >  2:3998284/1‑150 (MQ=255)
                 cGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGcgccg                                                                                                                                      >  2:273171/1‑150 (MQ=255)
                  gACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGcgccgc                                                                                                                                     >  1:706083/1‑150 (MQ=255)
                   aCGCCTTTTCGCTTGACCCGGTGCTGCAGACCCTGCGCATTCGGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTTTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCt                                                                                                                                    <  2:1685244/150‑1 (MQ=255)
                   aCGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCt                                                                                                                                    <  2:672383/150‑1 (MQ=255)
                     gCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTCTGGCAACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTgg                                                                                                                                  >  2:3986763/1‑150 (MQ=255)
                           gcGCTGGACCCGTTGCTGCAGACCCTGCCAATACCGTTCGACCACACCCTCGGCTCGCTCACCGCGCGCCGGGGATTCTTACGGCTCTTGGCCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCAc                                                                                                                            <  2:47824/149‑1 (MQ=255)
                              cTTGACCCGGAGCTGCAGACCCTGCGCTTTCCGTTCGCCGACACCCGCGGCTCGCTCACCGCGCGCCGGGGCTTCTTCCGGCTCTAGGGCACCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTc                                                                                                                         <  2:3807416/150‑1 (MQ=255)
                              cTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTc                                                                                                                         >  1:1690835/1‑150 (MQ=255)
                              cTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTc                                                                                                                         >  2:4272253/1‑150 (MQ=255)
                                   ccgggAGCTGCAGACCGTGCGATTTCCTTCCTACGACACCCTCGGCTCGCTCACCGCGCGCCGGGGCTTCTTACGGCTCTTTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCtt                                                                                                                    <  2:3415261/147‑1 (MQ=255)
                                            gCAGACCCGGCGCATACCGTTCGACTACACCCGCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGa                                                                                                           <  2:1743432/150‑1 (MQ=255)
                                                aCCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAAcg                                                                                                       >  2:3010012/1‑150 (MQ=255)
                                                   cTGCCCATTCCGATCGACGACCCCCGCGCCTCCAACCCGCCGGGCCGGGGCTTCTCACGCCTCTATGGCACCGACGCGCTCAAGTCCACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCgg                                                                                                    <  1:2093423/150‑1 (MQ=255)
                                                    tGCGCAGTCCGTTCGACGACACCCTCGGCTCGCTCCCCGCGCGCCCGGGCTTCTTCCGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGa                                                                                                   <  2:2923212/150‑1 (MQ=255)
                                                     gcgatttCCGTTCGACGACACCACCGGCTCGCTCACCGCGCGCCCTGGCTTCTTACGGCTCTCTGGCCACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGAt                                                                                                  <  2:3655497/145‑1 (MQ=255)
                                                         ttgccGTTCGACGACCCCCGCGGCTCGCTCCCCGCGCGCCCGGGCTTCTTCCGGCTCTAGGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAg                                                                                              <  2:1213115/147‑1 (MQ=255)
                                                         aTTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAg                                                                                              >  1:3925294/1‑150 (MQ=255)
                                                           tCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTGCGCCTTCCGGGCAGAAACGCGGATGGAGtt                                                                                            >  1:3420361/1‑150 (MQ=255)
                                                            ccGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtc                                                                                           >  1:316818/1‑150 (MQ=255)
                                                                  gacgacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCg                                                                                     >  1:1600348/1‑150 (MQ=255)
                                                                    cgacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGt                                                                                   >  2:1952394/1‑150 (MQ=255)
                                                                     gacACCCGCGGCACGCACACCGCGCGCCCGGGCTTCTACCGGCACTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTg                                                                                  <  2:2850041/150‑1 (MQ=255)
                                                                       cccccggggCCCGCCCCCCGGGCGCCGGGGCTTTTTCGGGACATATGCCAACGACTCGCACAATGCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCa                                                                                <  1:2674489/143‑1 (MQ=255)
                                                                       cacCCGCGGCTCGCACCCCGCGCGCCGGGGCTTCTTACGGCTCTTGGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCa                                                                                <  1:2872619/150‑1 (MQ=255)
                                                                          ccTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACtt                                                                             >  2:1006016/1‑150 (MQ=255)
                                                                           cTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTc                                                                            >  1:491130/1‑150 (MQ=255)
                                                                            tCGGCTCGCTCACCGCGCGCCCGGGCTGCTTCCGGCTATATGGCAACGACTCGCTCATTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTcc                                                                           <  1:4096169/150‑1 (MQ=255)
                                                                            ggggCTCGCTCCCCGCGCGCCCGGGCGTTTTACGGCTTTTTGGCAAGGACTCGCTCTTGTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTcc                                                                           <  1:2144142/148‑1 (MQ=255)
                                                                              ggCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcag                                                                         >  1:3810783/1‑150 (MQ=255)
                                                                              ggCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcag                                                                         >  1:1176970/1‑150 (MQ=255)
                                                                              ggCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcag                                                                         >  1:2054232/1‑150 (MQ=255)
                                                                               gctcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagc                                                                        >  1:2360477/1‑150 (MQ=255)
                                                                                ctcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagca                                                                       <  2:2254011/150‑1 (MQ=255)
                                                                                 tcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGAAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagcag                                                                      <  2:2486067/150‑1 (MQ=255)
                                                                                 tcgaaccccGCGCGCCGGGGTTATTAAGGGATATTTGGAAACGACTCGCTCAATTCGCCCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagcag                                                                      <  1:1263868/143‑1 (MQ=255)
                                                                                     tcACCGCGCGCCCGGGCTTCTTCCGGCTCTAGGGCAACGACTCGCTCAATTCGCCCTTCACCCCCTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                  <  2:2903950/150‑1 (MQ=255)
                                                                                     tcACCGCGCGCCCGGGCTTCTTACGGCACTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                  <  2:3735011/150‑1 (MQ=255)
                                                                                       aCCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGcgcg                                                                >  1:3180875/1‑150 (MQ=255)
                                                                                                cggggCTTATTACGGATATTTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                       <  1:1002332/148‑1 (MQ=255)
                                                                                                 ggggCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                      <  1:4004817/149‑1 (MQ=255)
                                                                                                 cGGGCTTCTTACGGCTCTTTGGCAACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                      <  2:3206424/150‑1 (MQ=255)
                                                                                                 cGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                      >  2:4129968/1‑150 (MQ=255)
                                                                                                 cGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                      >  1:1723657/1‑150 (MQ=255)
                                                                                                  gggCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTg                                                     <  2:2594183/150‑1 (MQ=255)
                                                                                                  gggCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTg                                                     >  1:2580167/1‑150 (MQ=255)
                                                                                                   ggCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGc                                                    >  2:3606311/1‑150 (MQ=255)
                                                                                                     cttcttCCGGCTCTAGGGCAACGACTCGCTCAATTCGACCTGCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                  <  2:3216523/150‑1 (MQ=255)
                                                                                                     cttcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                  >  1:647290/1‑150 (MQ=255)
                                                                                                     cttcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                  >  1:280303/1‑150 (MQ=255)
                                                                                                      ttcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctac                                                 >  1:249899/1‑150 (MQ=255)
                                                                                                       tcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                <  2:4408758/150‑1 (MQ=255)
                                                                                                       tcttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                >  1:2410003/1‑150 (MQ=255)
                                                                                                       tcttACGGCTCTAGGGCAACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                <  2:3861522/150‑1 (MQ=255)
                                                                                                        cttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctacta                                               >  2:2396472/1‑150 (MQ=255)
                                                                                                          tACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTaca                                             >  2:4512914/1‑150 (MQ=255)
                                                                                                          tACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTaca                                             >  2:3311706/1‑150 (MQ=255)
                                                                                                            cGGCTCTTGGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTacaac                                           <  2:2010189/150‑1 (MQ=255)
                                                                                                            cGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTccaac                                           >  1:59179/1‑150 (MQ=255)
                                                                                                            cGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTacaac                                           <  2:110735/150‑1 (MQ=255)
                                                                                                            cGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTacaac                                           >  1:2924628/1‑150 (MQ=255)
                                                                                                               ctctATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGc                                        >  1:3262787/1‑150 (MQ=255)
                                                                                                                  tATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaa                                     >  2:572042/1‑150 (MQ=255)
                                                                                                                  tATGGAAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaa                                     <  1:2221929/150‑1 (MQ=255)
                                                                                                                   aTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaa                                    >  1:1140266/1‑150 (MQ=255)
                                                                                                                   aTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaa                                    <  2:3099800/150‑1 (MQ=255)
                                                                                                                      gCAACGACTCGCTCAATTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACt                                 <  2:1939408/150‑1 (MQ=255)
                                                                                                                       cAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTg                                >  2:1416998/1‑150 (MQ=255)
                                                                                                                         aCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGa                              <  1:3998254/150‑1 (MQ=255)
                                                                                                                         aCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGa                              >  2:1526262/1‑150 (MQ=255)
                                                                                                                          cGACTCGCTCATTTCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACACCAGCAAAAACTGGAg                             <  1:2044080/150‑1 (MQ=255)
                                                                                                                          cGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAg                             >  1:4253946/1‑150 (MQ=255)
                                                                                                                          cGACTCGATCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAg                             <  2:2154496/150‑1 (MQ=255)
                                                                                                                            aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCt                           <  2:3975690/150‑1 (MQ=255)
                                                                                                                               cGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACt                        >  1:4236484/1‑150 (MQ=255)
                                                                                                                               cGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACt                        <  2:4373686/150‑1 (MQ=255)
                                                                                                                                   cAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCtt                    <  1:99922/150‑1 (MQ=255)
                                                                                                                                   cAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCtt                    >  1:104350/1‑150 (MQ=255)
                                                                                                                                    aaTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCttt                   >  2:4297348/1‑150 (MQ=255)
                                                                                                                                      ttCGACCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtgt                 <  1:4332723/150‑1 (MQ=255)
                                                                                                                                      ttCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtgt                 <  1:1084019/150‑1 (MQ=255)
                                                                                                                                        cGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTgg               >  2:1278108/1‑150 (MQ=255)
                                                                                                                                         gACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGa              >  1:2977010/1‑150 (MQ=255)
                                                                                                                                         gACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGa              >  1:3391490/1‑150 (MQ=255)
                                                                                                                                         gACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGa              >  1:3391508/1‑150 (MQ=255)
                                                                                                                                          aCCTTCACCCACTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGAc             <  1:4055528/150‑1 (MQ=255)
                                                                                                                                          aCCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCACAAACTGGAGCTACTGCTTTGTGGAc             >  2:753489/1‑150 (MQ=255)
                                                                                                                                                 cccAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTCCgagg      >  2:2785568/1‑150 (MQ=255)
                                                                                                                                                 cccAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg      <  2:1462376/150‑1 (MQ=255)
                                                                                                                                                    aaTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGaggagg   >  1:3412282/1‑150 (MQ=255)
                                                                                                                                                     aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTCCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAggg  >  2:135924/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGG  >  CP009273/283487‑283785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑