Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 14.5% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG14.5% 573.9 / 30.0 262E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (64/153);  new base G (42/3);  total (106/156)
Fisher's exact test for biased strand distribution p-value = 4.08e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCAC  >  CP009273/295547‑295844
                                                                                                                                                    |                                                                                                                                                     
cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                      >  1:2638336/1‑150 (MQ=255)
 gtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTc                                                                                                                                                     >  1:4482051/1‑150 (MQ=255)
   cgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCg                                                                                                                                                   <  2:2133469/150‑1 (MQ=255)
   cgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTccc                                                                                                                                                   >  1:3744068/1‑149 (MQ=255)
    gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACgg                                                                                                                                                  >  2:628519/1‑150 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGc                                                                                                                                                 >  2:3237232/1‑150 (MQ=255)
          gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgcc                                                                                                                                            <  1:740188/150‑1 (MQ=255)
            tttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGTgccgc                                                                                                                                          >  1:4029321/1‑150 (MQ=255)
             ttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccgcc                                                                                                                                         <  2:3570027/150‑1 (MQ=255)
               cGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCgg                                                                                                                                       <  2:880443/150‑1 (MQ=255)
               cGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCgg                                                                                                                                       <  2:3995490/150‑1 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCggg                                                                                                                                      >  2:1298548/1‑150 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCggg                                                                                                                                      <  2:3028620/150‑1 (MQ=255)
                 aGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCCGGc                                                                                                                                     >  2:3512342/1‑150 (MQ=255)
                  gcgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCa                                                                                                                                    <  1:3357526/150‑1 (MQ=255)
                   cgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAg                                                                                                                                   <  2:2928405/150‑1 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAgg                                                                                                                                  >  2:755178/1‑150 (MQ=255)
                     ccATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCCGGt                                                                                                                                 >  2:3166969/1‑150 (MQ=255)
                      cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCCCCCGGCAgggg                                                                                                                                >  2:468946/1‑148 (MQ=255)
                      cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGtt                                                                                                                                >  2:2467636/1‑150 (MQ=255)
                      cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGtt                                                                                                                                <  2:3640912/150‑1 (MQ=255)
                      cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGtt                                                                                                                                <  1:1115904/150‑1 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGttt                                                                                                                               <  2:4165368/150‑1 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTGTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGttt                                                                                                                               >  2:4481428/1‑150 (MQ=255)
                       aTCAGACAGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCacggtt                                                                                                                               >  2:1681035/1‑145 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCa                                                                                                                            <  2:3346978/150‑1 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCa                                                                                                                            <  1:1384197/150‑1 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCGCCGGGCAGGTTTCCa                                                                                                                            >  2:1879262/1‑150 (MQ=255)
                              cGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAcc                                                                                                                        <  2:3847647/150‑1 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCCCGTTTACAGAccc                                                                                                                       >  2:2382490/1‑149 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGTGCCGCCCGGGCGGGGTCCAGACCa                                                                                                                       >  1:3515057/1‑150 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAg                                                                                                                      <  1:3172747/150‑1 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCACGCGTGCCGCCCGGCAGGTTTGCAGAACac                                                                                                                      >  1:921988/1‑149 (MQ=255)
                                 gggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGc                                                                                                                     <  2:3296152/150‑1 (MQ=255)
                                    cTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCttt                                                                                                                  <  1:1903951/150‑1 (MQ=255)
                                     ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                                 <  1:2178801/150‑1 (MQ=255)
                                     ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                                 <  1:749863/150‑1 (MQ=255)
                                      tCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCa                                                                                                                <  1:3075030/150‑1 (MQ=255)
                                       cGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGGGGGGCGGCCGGCCGGGTTGCACACCAGCattgaa                                                                                                               >  1:19888/1‑147 (MQ=255)
                                        gCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtg                                                                                                              <  1:3308162/150‑1 (MQ=255)
                                         ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCGGGCAGGTTTACAGACCACCGTTCAagt                                                                                                             >  1:1025451/1‑150 (MQ=255)
                                              cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGAt                                                                                                        <  2:2639141/150‑1 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAg                                                                                                      <  2:2912570/150‑1 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGGCCCCCGGCCGGTGTGCAGAACAGCGTTAAGGTGAGATat                                                                                                      >  2:787344/1‑149 (MQ=255)
                                                 acgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGc                                                                                                     <  2:3497965/150‑1 (MQ=255)
                                                 acgcaTGGCGGTCCCTTCCGGCAACTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGc                                                                                                     <  2:4093998/150‑1 (MQ=255)
                                                  cgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCg                                                                                                    <  1:2593066/150‑1 (MQ=255)
                                                   gcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCgg                                                                                                   <  1:1496062/150‑1 (MQ=255)
                                                       ggCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAt                                                                                               <  1:3115366/150‑1 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCg                                                                                        <  1:4457343/150‑1 (MQ=255)
                                                                  ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                    <  2:147010/150‑1 (MQ=255)
                                                                   cGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTg                                                                                   <  2:2009756/150‑1 (MQ=255)
                                                                    ggCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTgg                                                                                  <  1:2440472/150‑1 (MQ=255)
                                                                      cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTc                                                                                <  1:443085/150‑1 (MQ=255)
                                                                      cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTc                                                                                <  2:4358273/150‑1 (MQ=255)
                                                                      cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTc                                                                                <  2:1265209/150‑1 (MQ=255)
                                                                      cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGCGCCGCCCGGCCGGGTTACAGAACAACTTTAAAGTGAGAAAGCCGTGATTTTACCGCTCTGGTc                                                                                >  2:3071677/1‑150 (MQ=255)
                                                                       aaaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGGGCCCCCGGGCAGGTTTCCACACCAGCGTTCCTTGGTGATAGCGGGGATTTTACCGCGCAGGTCg                                                                               >  2:4313001/1‑150 (MQ=255)
                                                                            aaGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCCGCGGGCCGCCGGGCCCGTTTGCAGACCAGCAGTAAAGTGTGATAGCGGTGTTTTTAACGCGCTGGGCGGcccc                                                                          >  1:2908193/1‑150 (MQ=255)
                                                                                        ccGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTg                                                              <  2:2306863/150‑1 (MQ=255)
                                                                                              gCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCCGGTTTGCAGACCCCCGTTAATGTGTGATAGCGGTGATTTTACCGCGCGGGTCGGCCCCGATAAGCAAGTGCTGAAg                                                        >  1:2573312/1‑150 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGt                                                     <  2:1451202/150‑1 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCACCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGt                                                     <  1:2743518/150‑1 (MQ=255)
                                                                                                   tATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                   <  1:3321601/150‑1 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAACAGCGTTCATGTGAGATAGCGGTGATTTTCCCCCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcg                                                 >  2:4045470/1‑150 (MQ=255)
                                                                                                      gACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGCTTTTCCCGCGCAGGTCGGCACCCATACGCAAGTTCAGAAGGGTggcgg                                                >  2:2113231/1‑150 (MQ=255)
                                                                                                        cGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGGGCCGCCCGGCAGGTTTCCAGACCAGCATTCATGTGAGATAGCGGTGATTTTACCCCGCAGGTCGGCCCCGATAAGCAAGGGCTGAAGGGGggcgggg                                              >  2:1344565/1‑150 (MQ=255)
                                                                                                           ttCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCGTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATAAGCAAGTGCTGAAGGGTggcggggcgg                                           >  2:294952/1‑150 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGAACACCTTTCATGGGAGATAGACGTGATTTTACCGCACAGGGAGGCAACGATACGCACTTGCTGTAGGGGggcgggggcggg                                         >  2:704471/1‑145 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCCGCGTGCCCCCGGGCAGGTTTACAGACCAGCGTTCAAGTGAGATATCGGGGAGTTTAACGCGCAGGTCGGCCCCCAGACGCAAGTGCAGAAGGGGGGCGGGGCGGTGc                                        >  1:263365/1‑150 (MQ=255)
                                                                                                               ccccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCg                                       <  1:186989/150‑1 (MQ=255)
                                                                                                               ccccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCg                                       <  1:2451602/150‑1 (MQ=255)
                                                                                                                   ggCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                   <  2:1004691/150‑1 (MQ=255)
                                                                                                                      gTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGtt                                <  2:3369458/150‑1 (MQ=255)
                                                                                                                         gagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                             <  2:1494029/150‑1 (MQ=255)
                                                                                                                         gagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                             <  1:3029829/150‑1 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCGCCCGGGCGGTGTGCAGACCAGCGTTCATGTGAGGTCGCGGGGATTTTACCGCGCTGGTCGGCCCCGATACGCACCTGGTGCGGGGGGGCGGGGCGGGGGGGGGTGGTTTTg                            >  2:913105/1‑150 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCCGCGTGCCGCCCGGCAGGTTTCCAGACCAGCGTTCATGTGAGATATCGGTGATTTTACCGCTCGGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                            >  2:4064544/1‑150 (MQ=255)
                                                                                                                            tAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTggg                          <  2:2208736/150‑1 (MQ=255)
                                                                                                                             aaTTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGa                         <  1:3628743/150‑1 (MQ=255)
                                                                                                                             aaTTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATACGCAAGTGCTGAATGGTGGCGGGGCTGTGCGGGGTGTTATTGGGa                         >  2:1801449/1‑150 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                      <  1:3965383/150‑1 (MQ=255)
                                                                                                                                 cGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATag                     <  1:80637/150‑1 (MQ=255)
                                                                                                                                  gCTTTGCTGTACCGCCGTGTACGGCGTGCCGCCGGGCAGGTTTACAGACCAGCATTAATGTGAGATATCGGTGATTTTACCGCACAGGTCGGCACCCAAAAGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGGGGGGGTATTGGGAATaga                    >  2:4468671/1‑150 (MQ=255)
                                                                                                                                   cTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATagag                   <  1:864156/150‑1 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAgg                  <  2:3451159/150‑1 (MQ=255)
                                                                                                                                     ttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAggg                 <  1:756184/150‑1 (MQ=255)
                                                                                                                                     ttGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAggg                 <  1:3237232/150‑1 (MQ=255)
                                                                                                                                        cTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTACAGACCACCTTTAATGTGAGATATCGGTGTTTTTACCGCTGTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGGGGTGTTTTTGGGAATAGAGGGGCg              >  2:1712321/1‑150 (MQ=255)
                                                                                                                                          gTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggg            <  2:797811/150‑1 (MQ=255)
                                                                                                                                          gTACCGCCGTGTGCGGCGTGCCGCCGGGCAGGTTTACAGACCAGCTTTGATGTGTGATATCGGTGTTTTTACCGCTCTGGTCGGCACCGATAAGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATATAGGGGCggg            >  2:2986924/1‑150 (MQ=255)
                                                                                                                                           tACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCgggg           <  1:4303113/150‑1 (MQ=255)
                                                                                                                                            aCCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg          <  1:138859/150‑1 (MQ=255)
                                                                                                                                              cgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAg        <  2:3850209/150‑1 (MQ=255)
                                                                                                                                               gccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGc       >  1:664447/1‑150 (MQ=255)
                                                                                                                                                ccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCa      >  2:2534707/1‑150 (MQ=255)
                                                                                                                                                 cgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGTTAGCGGTGAGTTTCCCGCTCTGGTCGGCACCGATACGCAAGTTCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTTTTGGGAATAGAGGGGCGGGGGAGCAt     <  2:4434232/150‑1 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc    >  1:2049589/1‑150 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc    <  2:1910778/150‑1 (MQ=255)
                                                                                                                                                   tttCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCa   <  1:54267/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCAC  >  CP009273/295547‑295844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑