Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,101,591 A→T 16.0% F308L (TTT→TTA)  speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,101,5910AT16.0% 756.8 / 117.9 261F308L (TTT→TTA) speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (112/107);  new base T (22/20);  total (134/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

ATCTGCGACGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTG  >  CP009273/3101442‑3101740
                                                                                                                                                     |                                                                                                                                                     
aTCTGCGACGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGCCTGAGTCACAAAGAACCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCa                                                                                                                                                       <  1:1159440/150‑1 (MQ=255)
       aCGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGaa                                                                                                                                                >  1:3700863/1‑150 (MQ=255)
        cGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCCCGCGGAATCAAACAGAAt                                                                                                                                               >  1:3100012/1‑150 (MQ=255)
        cGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAAt                                                                                                                                               >  1:3099915/1‑150 (MQ=255)
        cGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAAt                                                                                                                                               >  1:280391/1‑150 (MQ=255)
         gTCTGTGATAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATg                                                                                                                                              <  2:2558128/150‑1 (MQ=255)
         gTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATg                                                                                                                                              >  2:2701124/1‑150 (MQ=255)
         gTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATg                                                                                                                                              >  2:2331352/1‑150 (MQ=255)
           cTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTa                                                                                                                                            <  1:2584455/150‑1 (MQ=255)
             gtgAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAAt                                                                                                                                          <  1:920273/150‑1 (MQ=255)
                agaATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACATAATGTAATcac                                                                                                                                       <  2:3901787/150‑1 (MQ=255)
                 gaATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATcaca                                                                                                                                      >  2:1136642/1‑150 (MQ=255)
                 gaATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAACAACCCCGGATCGTTTTCGTTAAGTTCAAACAGCAGCGGCGAGCTATCCGCCATCCTCGGGGTAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATcaca                                                                                                                                      >  1:1189633/1‑150 (MQ=255)
                  aaTCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATACGCCATCATCGGGATAAATTGTTCATAAACGACCCACGCGGAATCAAAAAGAATGTAATcacac                                                                                                                                     >  1:767889/1‑150 (MQ=255)
                   aTCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCAGGATCGTTTTAGGTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCAACATCGGGATAAATTGTTCAAAACCGACCCACGCGGAATCAAACAGAATGTAATcacaca                                                                                                                                    >  1:1329608/1‑150 (MQ=255)
                   aTCCCGCCTGCTGTTTGTGCAACGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATcacaca                                                                                                                                    <  1:4152586/150‑1 (MQ=255)
                          ctgctgTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGccc                                                                                                                             <  1:2078484/150‑1 (MQ=255)
                             ctgTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAAc                                                                                                                          <  1:2064614/150‑1 (MQ=255)
                              tgTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAACTTGTTCAAAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCACCg                                                                                                                         >  2:1428511/1‑150 (MQ=255)
                              tgTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACg                                                                                                                         >  2:2622247/1‑150 (MQ=255)
                              tgTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCAAACg                                                                                                                         >  2:358320/1‑150 (MQ=255)
                               gTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACgg                                                                                                                        >  2:4143312/1‑150 (MQ=255)
                                  tgtgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTAt                                                                                                                     <  2:689811/150‑1 (MQ=255)
                                   gtgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATc                                                                                                                    <  2:1272551/150‑1 (MQ=255)
                                    tgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCg                                                                                                                   >  1:4383378/1‑150 (MQ=255)
                                    tgCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCAGCATCGGGATAAATTGTTCATAACCGACCCCCGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCg                                                                                                                   >  1:4274435/1‑150 (MQ=255)
                                        ccGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCa                                                                                                               <  1:706954/150‑1 (MQ=255)
                                           aCTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCt                                                                                                            >  2:260766/1‑150 (MQ=255)
                                           aCTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCt                                                                                                            >  2:1557955/1‑150 (MQ=255)
                                           aCTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCAGCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCt                                                                                                            >  2:3759256/1‑150 (MQ=255)
                                                gTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACgg                                                                                                       >  1:3007832/1‑150 (MQ=255)
                                                 tCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGAGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAAACACACAGATGCCCAACCGACTCGATCACCTGACggg                                                                                                      >  1:1883213/1‑150 (MQ=255)
                                                      aaGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCGATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTta                                                                                                 <  2:557027/150‑1 (MQ=255)
                                                       aGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTtat                                                                                                >  1:2266582/1‑150 (MQ=255)
                                                       aGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTtat                                                                                                >  1:285613/1‑150 (MQ=255)
                                                          tCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCAGACGGGCGTtatata                                                                                             >  1:1056955/1‑148 (MQ=255)
                                                           ccccGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGAc                                                                                            >  2:3300999/1‑150 (MQ=255)
                                                            cccGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACa                                                                                           >  1:2257016/1‑150 (MQ=255)
                                                                 aTCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGcc                                                                                      >  1:3167503/1‑150 (MQ=255)
                                                                   cGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGt                                                                                    >  1:2946368/1‑150 (MQ=255)
                                                                   cGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGt                                                                                    >  2:1600720/1‑150 (MQ=255)
                                                                        tCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAg                                                                               <  2:2766252/150‑1 (MQ=255)
                                                                        tCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAg                                                                               <  2:1805000/150‑1 (MQ=255)
                                                                          gTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGACTGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGt                                                                             >  2:3343884/1‑150 (MQ=255)
                                                                          gTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGt                                                                             >  2:521084/1‑150 (MQ=255)
                                                                           ttAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGtt                                                                            >  2:3080171/1‑150 (MQ=255)
                                                                            tAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTc                                                                           <  2:3824392/150‑1 (MQ=255)
                                                                             aaGTTCTAACAGCAGCGGCTAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTcc                                                                          <  2:1638823/150‑1 (MQ=255)
                                                                             aaGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTcc                                                                          >  1:1198772/1‑150 (MQ=255)
                                                                             aaGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTcc                                                                          <  2:2078420/150‑1 (MQ=255)
                                                                                 tCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGc                                                                      >  1:4522265/1‑150 (MQ=255)
                                                                                  cTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCt                                                                     >  2:918283/1‑150 (MQ=255)
                                                                                   tAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTg                                                                    <  1:2682558/150‑1 (MQ=255)
                                                                                       agcagcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGaata                                                                >  1:3401633/1‑150 (MQ=255)
                                                                                          agcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATc                                                             <  2:478539/150‑1 (MQ=255)
                                                                                          agcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATc                                                             <  2:1154729/150‑1 (MQ=255)
                                                                                          agcGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATc                                                             >  2:4403705/1‑150 (MQ=255)
                                                                                           gcggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCg                                                            <  1:1518837/150‑1 (MQ=255)
                                                                                             ggcgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGcc                                                          >  2:2523414/1‑150 (MQ=255)
                                                                                               cgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCTGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAg                                                        <  2:2804219/150‑1 (MQ=255)
                                                                                               cgAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAg                                                        >  1:2652323/1‑150 (MQ=255)
                                                                                                gAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAgg                                                       <  1:1838402/150‑1 (MQ=255)
                                                                                                  gCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCg                                                     <  1:4227475/150‑1 (MQ=255)
                                                                                                   cTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGa                                                    >  1:880122/1‑150 (MQ=255)
                                                                                                   cTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGa                                                    <  2:1287672/150‑1 (MQ=255)
                                                                                                    tATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGAt                                                   <  1:2129321/150‑1 (MQ=255)
                                                                                                     aTCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATa                                                  >  2:1484791/1‑150 (MQ=255)
                                                                                                      tCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATAc                                                 <  2:2912032/150‑1 (MQ=255)
                                                                                                       ccGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACg                                                <  2:2837236/150‑1 (MQ=255)
                                                                                                        cGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACgg                                               >  1:2826037/1‑150 (MQ=255)
                                                                                                         gCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACggg                                              >  2:1517768/1‑150 (MQ=255)
                                                                                                         gCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACggg                                              >  2:3913751/1‑150 (MQ=255)
                                                                                                           catcatCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGgcg                                            <  2:2940307/150‑1 (MQ=255)
                                                                                                           catcatCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGgcg                                            <  2:2940895/150‑1 (MQ=255)
                                                                                                                tcGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCa                                       <  1:3536095/150‑1 (MQ=255)
                                                                                                                tcGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCa                                       >  2:4114156/1‑150 (MQ=255)
                                                                                                                  gggATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCTATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAgg                                     >  2:4176504/1‑150 (MQ=255)
                                                                                                                  gggATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAgg                                     <  1:3080171/150‑1 (MQ=255)
                                                                                                                   ggATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCTATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATACTCGCCAGGCGATACGGGCGCGGCAGGt                                    <  2:3554652/150‑1 (MQ=255)
                                                                                                                    gATAAATTGTTCATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTc                                   >  2:3110074/1‑150 (MQ=255)
                                                                                                                    gATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTc                                   <  2:3970189/150‑1 (MQ=255)
                                                                                                                    gATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTc                                   >  1:334387/1‑150 (MQ=255)
                                                                                                                     aTAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCg                                  <  2:1038745/150‑1 (MQ=255)
                                                                                                                        aaTTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCt                               >  1:4426841/1‑150 (MQ=255)
                                                                                                                         aTTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCtt                              >  2:3145214/1‑150 (MQ=255)
                                                                                                                            gTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCttttt                           >  1:487099/1‑150 (MQ=255)
                                                                                                                             ttCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTc                          <  2:4446926/150‑1 (MQ=255)
                                                                                                                              tCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCt                         <  2:1321843/150‑1 (MQ=255)
                                                                                                                              tCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCt                         <  1:252722/150‑1 (MQ=255)
                                                                                                                              tCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCt                         <  2:2832523/150‑1 (MQ=255)
                                                                                                                               cATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTg                        >  1:2495746/1‑150 (MQ=255)
                                                                                                                               cATAACCGACCCACGCGGAATCTAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTg                        >  1:3937694/1‑150 (MQ=255)
                                                                                                                                aTAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTgg                       >  2:773247/1‑150 (MQ=255)
                                                                                                                                  aaCCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGgcg                     <  2:2391558/150‑1 (MQ=255)
                                                                                                                                  aaCCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGgcg                     <  2:1757309/150‑1 (MQ=255)
                                                                                                                                         ccACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTc              <  1:498862/150‑1 (MQ=255)
                                                                                                                                         ccACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTc              >  1:739013/1‑150 (MQ=255)
                                                                                                                                            cgcgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTcgcg           <  2:632022/150‑1 (MQ=255)
                                                                                                                                              cgGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGaa         >  1:3852474/1‑150 (MQ=255)
                                                                                                                                                  aTCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTg     <  1:173989/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTGCGACGTCTGTGAGAATCCCGCCTGCTGTTTGTGCACCGACTGAGTCACAAAGATCCCCGGATCGTTTTCGTTAAGTTCTAACAGCAGCGGCGAGCTATCCGCCATCATCGGGATAAATTGTTCATAACCGACCCACGCGGAATCAAACAGAATGTAATCACACAGATGCCCAACGGTATCGATCACCTGACGGGCGTTATAGACAGTGCCGTCATAGGTTCCCAGCTGAATAATCGCCAGGCGATACGGGCGCGGCAGGTCGGCTTTTTCTGGCGCAACGTCGCGAATTTGCTG  >  CP009273/3101442‑3101740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑